Rhizorhabdus dicambivorans
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4856 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A4FZA5|A0A2A4FZA5_9SPHN TonB-dependent receptor OS=Rhizorhabdus dicambivorans OX=1850238 GN=COO09_07955 PE=3 SV=1
MM1 pKa = 7.19 STPTSNSVTAEE12 pKa = 4.28 RR13 pKa = 11.84 ITLPADD19 pKa = 3.88 PDD21 pKa = 4.02 LSSLTHH27 pKa = 5.92 GSRR30 pKa = 11.84 WVATGATTSLTYY42 pKa = 10.58 SFPINAGNWANPYY55 pKa = 10.45 SPLNEE60 pKa = 4.17 PSTGGVIPLDD70 pKa = 3.43 AAAATGAVTALQLWSRR86 pKa = 11.84 YY87 pKa = 10.03 ANISFAATSDD97 pKa = 3.07 TGANVGDD104 pKa = 3.33 IRR106 pKa = 11.84 FAYY109 pKa = 9.08 TRR111 pKa = 11.84 DD112 pKa = 3.19 EE113 pKa = 4.3 DD114 pKa = 4.3 AAAHH118 pKa = 6.76 AYY120 pKa = 10.15 LPSSGPAAGDD130 pKa = 2.75 IWLDD134 pKa = 3.36 STTDD138 pKa = 3.55 FGGFEE143 pKa = 4.08 RR144 pKa = 11.84 GSFGLFTLLHH154 pKa = 5.89 EE155 pKa = 4.85 VGHH158 pKa = 6.45 ALGLKK163 pKa = 10.15 HH164 pKa = 6.45 PFEE167 pKa = 5.32 SSTLNEE173 pKa = 4.17 QILDD177 pKa = 3.89 PEE179 pKa = 4.67 LDD181 pKa = 4.0 SVNSTVMSYY190 pKa = 11.21 NLYY193 pKa = 10.65 SDD195 pKa = 5.69 LPASDD200 pKa = 4.44 YY201 pKa = 11.23 GISYY205 pKa = 10.56 LPTTPMQLDD214 pKa = 3.88 IEE216 pKa = 4.54 AVQALYY222 pKa = 10.47 GARR225 pKa = 11.84 PFNTDD230 pKa = 2.89 DD231 pKa = 3.46 NEE233 pKa = 4.42 YY234 pKa = 10.38 VFNSGSQYY242 pKa = 10.75 FEE244 pKa = 4.52 TIYY247 pKa = 11.11 DD248 pKa = 3.51 SGGNDD253 pKa = 3.4 TIIWNSSTEE262 pKa = 3.85 EE263 pKa = 3.75 AVIDD267 pKa = 4.33 LEE269 pKa = 4.46 SGSWSALGDD278 pKa = 3.66 PLITFDD284 pKa = 4.18 RR285 pKa = 11.84 QGEE288 pKa = 4.6 VVKK291 pKa = 10.89 VDD293 pKa = 3.68 YY294 pKa = 8.52 FTVAIFSEE302 pKa = 4.37 TMIEE306 pKa = 4.04 NATGGGGDD314 pKa = 3.85 DD315 pKa = 5.31 LIYY318 pKa = 11.14 GNAVANRR325 pKa = 11.84 LLGSDD330 pKa = 3.21 GHH332 pKa = 7.84 DD333 pKa = 4.56 DD334 pKa = 3.19 IFGYY338 pKa = 10.57 EE339 pKa = 4.16 GADD342 pKa = 3.49 TLVGGAGNDD351 pKa = 3.54 HH352 pKa = 7.08 LYY354 pKa = 10.95 GRR356 pKa = 11.84 AASGGADD363 pKa = 3.77 GEE365 pKa = 4.72 DD366 pKa = 4.05 SLSGGAGSDD375 pKa = 3.62 YY376 pKa = 11.17 LQGNAGNDD384 pKa = 3.44 MLDD387 pKa = 3.51 GGEE390 pKa = 4.11 GSDD393 pKa = 4.35 RR394 pKa = 11.84 INGGGSDD401 pKa = 3.74 DD402 pKa = 5.18 SIFGGAGNDD411 pKa = 3.61 TVNGNLGSDD420 pKa = 4.16 VISGGDD426 pKa = 3.35 GDD428 pKa = 4.34 DD429 pKa = 3.89 SLRR432 pKa = 11.84 GGQGADD438 pKa = 3.71 SISGGTGNDD447 pKa = 3.2 ILSGDD452 pKa = 4.03 LGEE455 pKa = 6.02 DD456 pKa = 3.1 ILSGGDD462 pKa = 3.52 GNDD465 pKa = 3.26 LFVVGGSGSPLAQPDD480 pKa = 4.16 RR481 pKa = 11.84 ILDD484 pKa = 3.87 FTDD487 pKa = 3.48 GTDD490 pKa = 2.86 WVAIGFAPAAILTGAAQANAGAAATTAQQLLDD522 pKa = 3.54 NRR524 pKa = 11.84 EE525 pKa = 4.17 GNGEE529 pKa = 4.01 VAALAVGADD538 pKa = 3.34 TYY540 pKa = 11.59 LFYY543 pKa = 11.08 ASNGGGMIDD552 pKa = 3.41 SAIVVVGIAPQAFGVADD569 pKa = 4.7 FGG571 pKa = 4.47
Molecular weight: 58.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.554
IPC_protein 3.605
Toseland 3.363
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.274
Solomon 3.605
Lehninger 3.567
Nozaki 3.719
DTASelect 4.012
Thurlkill 3.427
EMBOSS 3.567
Sillero 3.732
Patrickios 0.896
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.685
Protein with the highest isoelectric point:
>tr|A0A2A4FRD8|A0A2A4FRD8_9SPHN MFS transporter OS=Rhizorhabdus dicambivorans OX=1850238 GN=COO09_17390 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.61 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 SATPGGRR28 pKa = 11.84 KK29 pKa = 9.04 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4856
0
4856
1542366
22
4544
317.6
34.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.227 ± 0.052
0.825 ± 0.011
5.958 ± 0.027
5.444 ± 0.036
3.529 ± 0.018
9.002 ± 0.057
2.049 ± 0.019
5.297 ± 0.02
2.892 ± 0.031
9.945 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.016
2.454 ± 0.025
5.35 ± 0.025
3.038 ± 0.018
7.712 ± 0.041
5.3 ± 0.026
4.984 ± 0.029
6.924 ± 0.03
1.398 ± 0.016
2.227 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here