Lactococcus phage 38502
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8BKA8|A0A1P8BKA8_9CAUD Uncharacterized protein OS=Lactococcus phage 38502 OX=1868846 GN=DS38502_24 PE=4 SV=1
MM1 pKa = 7.23 KK2 pKa = 10.11 KK3 pKa = 8.64 YY4 pKa = 9.83 WVVEE8 pKa = 3.9 DD9 pKa = 3.74 HH10 pKa = 6.88 LGGGFYY16 pKa = 10.8 LMPEE20 pKa = 4.1 DD21 pKa = 4.04 TPEE24 pKa = 3.89 EE25 pKa = 3.99 EE26 pKa = 4.03 LRR28 pKa = 11.84 EE29 pKa = 4.04 VEE31 pKa = 5.18 VYY33 pKa = 10.96 CDD35 pKa = 3.23 TCGDD39 pKa = 3.54 NDD41 pKa = 4.7 SIIGQFSNWNQLKK54 pKa = 10.45 KK55 pKa = 11.08 EE56 pKa = 4.06 MTDD59 pKa = 3.5 DD60 pKa = 4.56 EE61 pKa = 5.2 GWCPYY66 pKa = 10.28 SDD68 pKa = 5.14 EE69 pKa = 4.2 YY70 pKa = 10.46 LQSVFEE76 pKa = 4.68 EE77 pKa = 4.75 DD78 pKa = 3.42 NQQ80 pKa = 3.87
Molecular weight: 9.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.834
IPC_protein 3.757
Toseland 3.567
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.617
EMBOSS 3.656
Sillero 3.872
Patrickios 0.54
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A1P8BKB6|A0A1P8BKB6_9CAUD Portal protein OS=Lactococcus phage 38502 OX=1868846 GN=DS38502_38 PE=4 SV=1
MM1 pKa = 7.27 KK2 pKa = 10.49 SSWKK6 pKa = 9.83 KK7 pKa = 9.38 QRR9 pKa = 11.84 QAAKK13 pKa = 9.81 KK14 pKa = 10.21 RR15 pKa = 11.84 KK16 pKa = 8.9 IKK18 pKa = 10.22 CLRR21 pKa = 11.84 IKK23 pKa = 10.43 HH24 pKa = 5.93 RR25 pKa = 11.84 LIKK28 pKa = 10.26 RR29 pKa = 11.84 YY30 pKa = 9.72 APNIEE35 pKa = 4.12 TFIKK39 pKa = 10.16 LFNGIKK45 pKa = 10.05 HH46 pKa = 5.55 INGYY50 pKa = 9.49 RR51 pKa = 11.84 KK52 pKa = 9.5 SFYY55 pKa = 10.43 RR56 pKa = 11.84 YY57 pKa = 6.53 WQKK60 pKa = 9.72 LTSIKK65 pKa = 10.69 YY66 pKa = 10.26 NSFSYY71 pKa = 11.04
Molecular weight: 8.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 10.438
IPC_protein 10.965
Toseland 11.096
ProMoST 10.745
Dawson 11.169
Bjellqvist 10.847
Wikipedia 11.359
Rodwell 11.637
Grimsley 11.213
Solomon 11.272
Lehninger 11.242
Nozaki 11.067
DTASelect 10.847
Thurlkill 11.082
EMBOSS 11.491
Sillero 11.096
Patrickios 11.359
IPC_peptide 11.286
IPC2_peptide 9.692
IPC2.peptide.svr19 7.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
10388
43
864
173.1
19.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.122 ± 0.441
0.741 ± 0.125
5.968 ± 0.283
6.681 ± 0.501
4.39 ± 0.18
6.575 ± 0.55
1.454 ± 0.131
7.21 ± 0.308
9.136 ± 0.61
7.942 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.174
6.18 ± 0.397
3.052 ± 0.174
4.245 ± 0.378
3.639 ± 0.318
7.018 ± 0.365
6.257 ± 0.504
5.824 ± 0.249
1.357 ± 0.141
3.677 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here