Amino acid dipepetide frequency for Spirobacillus cienkowskii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.614AlaAla: 4.614 ± 0.107	0.736AlaCys: 0.736 ± 0.034	2.501AlaAsp: 2.501 ± 0.057	3.674AlaGlu: 3.674 ± 0.065	2.913AlaPhe: 2.913 ± 0.069	3.475AlaGly: 3.475 ± 0.078	1.276AlaHis: 1.276 ± 0.044	5.222AlaIle: 5.222 ± 0.087	5.068AlaLys: 5.068 ± 0.082	6.265AlaLeu: 6.265 ± 0.113	1.396AlaMet: 1.396 ± 0.045	3.193AlaAsn: 3.193 ± 0.065	1.9AlaPro: 1.9 ± 0.059	2.619AlaGln: 2.619 ± 0.058	2.44AlaArg: 2.44 ± 0.057	4.157AlaSer: 4.157 ± 0.083	3.254AlaThr: 3.254 ± 0.093	3.543AlaVal: 3.543 ± 0.082	0.58AlaTrp: 0.58 ± 0.026	1.764AlaTyr: 1.764 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.824CysAla: 0.824 ± 0.038	0.15CysCys: 0.15 ± 0.013	0.635CysAsp: 0.635 ± 0.031	0.733CysGlu: 0.733 ± 0.033	0.762CysPhe: 0.762 ± 0.028	0.793CysGly: 0.793 ± 0.03	0.239CysHis: 0.239 ± 0.017	0.988CysIle: 0.988 ± 0.035	0.819CysLys: 0.819 ± 0.033	1.239CysLeu: 1.239 ± 0.044	0.222CysMet: 0.222 ± 0.015	0.658CysAsn: 0.658 ± 0.029	0.453CysPro: 0.453 ± 0.025	0.414CysGln: 0.414 ± 0.022	0.4CysArg: 0.4 ± 0.021	0.904CysSer: 0.904 ± 0.034	0.541CysThr: 0.541 ± 0.035	0.721CysVal: 0.721 ± 0.028	0.11CysTrp: 0.11 ± 0.017	0.493CysTyr: 0.493 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	2.77AspAla: 2.77 ± 0.068	0.501AspCys: 0.501 ± 0.024	2.22AspAsp: 2.22 ± 0.067	3.036AspGlu: 3.036 ± 0.069	3.137AspPhe: 3.137 ± 0.074	2.376AspGly: 2.376 ± 0.064	0.875AspHis: 0.875 ± 0.032	4.056AspIle: 4.056 ± 0.074	3.702AspLys: 3.702 ± 0.068	4.771AspLeu: 4.771 ± 0.08	0.82AspMet: 0.82 ± 0.031	2.692AspAsn: 2.692 ± 0.058	1.724AspPro: 1.724 ± 0.052	1.188AspGln: 1.188 ± 0.042	1.53AspArg: 1.53 ± 0.05	3.279AspSer: 3.279 ± 0.066	2.037AspThr: 2.037 ± 0.052	2.704AspVal: 2.704 ± 0.057	0.518AspTrp: 0.518 ± 0.028	1.85AspTyr: 1.85 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	3.486GluAla: 3.486 ± 0.072	0.671GluCys: 0.671 ± 0.026	2.521GluAsp: 2.521 ± 0.057	3.897GluGlu: 3.897 ± 0.087	3.367GluPhe: 3.367 ± 0.066	2.693GluGly: 2.693 ± 0.07	1.318GluHis: 1.318 ± 0.04	6.258GluIle: 6.258 ± 0.099	6.639GluLys: 6.639 ± 0.104	6.051GluLeu: 6.051 ± 0.089	1.353GluMet: 1.353 ± 0.037	5.369GluAsn: 5.369 ± 0.091	1.557GluPro: 1.557 ± 0.055	2.283GluGln: 2.283 ± 0.054	2.473GluArg: 2.473 ± 0.066	3.846GluSer: 3.846 ± 0.065	3.059GluThr: 3.059 ± 0.061	3.531GluVal: 3.531 ± 0.064	0.528GluTrp: 0.528 ± 0.029	2.103GluTyr: 2.103 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.333PheAla: 3.333 ± 0.06	0.831PheCys: 0.831 ± 0.03	2.943PheAsp: 2.943 ± 0.064	3.393PheGlu: 3.393 ± 0.073	3.48PhePhe: 3.48 ± 0.086	3.148PheGly: 3.148 ± 0.071	1.011PheHis: 1.011 ± 0.033	4.971PheIle: 4.971 ± 0.097	3.992PheLys: 3.992 ± 0.076	6.06PheLeu: 6.06 ± 0.107	0.974PheMet: 0.974 ± 0.037	3.444PheAsn: 3.444 ± 0.079	1.97PhePro: 1.97 ± 0.049	1.89PheGln: 1.89 ± 0.055	1.631PheArg: 1.631 ± 0.045	5.12PheSer: 5.12 ± 0.101	2.418PheThr: 2.418 ± 0.057	3.178PheVal: 3.178 ± 0.066	0.59PheTrp: 0.59 ± 0.03	2.368PheTyr: 2.368 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	3.636GlyAla: 3.636 ± 0.083	0.718GlyCys: 0.718 ± 0.032	2.351GlyAsp: 2.351 ± 0.065	2.955GlyGlu: 2.955 ± 0.075	3.259GlyPhe: 3.259 ± 0.068	3.426GlyGly: 3.426 ± 0.105	1.037GlyHis: 1.037 ± 0.04	4.871GlyIle: 4.871 ± 0.094	4.334GlyLys: 4.334 ± 0.08	4.979GlyLeu: 4.979 ± 0.085	1.273GlyMet: 1.273 ± 0.047	2.812GlyAsn: 2.812 ± 0.075	1.1GlyPro: 1.1 ± 0.039	1.595GlyGln: 1.595 ± 0.052	1.991GlyArg: 1.991 ± 0.064	3.575GlySer: 3.575 ± 0.073	2.816GlyThr: 2.816 ± 0.067	3.676GlyVal: 3.676 ± 0.083	0.568GlyTrp: 0.568 ± 0.03	1.912GlyTyr: 1.912 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.261HisAla: 1.261 ± 0.04	0.278HisCys: 0.278 ± 0.021	0.958HisAsp: 0.958 ± 0.036	1.292HisGlu: 1.292 ± 0.042	1.194HisPhe: 1.194 ± 0.038	1.119HisGly: 1.119 ± 0.037	0.539HisHis: 0.539 ± 0.029	1.502HisIle: 1.502 ± 0.042	1.353HisLys: 1.353 ± 0.041	1.883HisLeu: 1.883 ± 0.055	0.346HisMet: 0.346 ± 0.021	1.177HisAsn: 1.177 ± 0.04	0.869HisPro: 0.869 ± 0.034	0.648HisGln: 0.648 ± 0.033	0.69HisArg: 0.69 ± 0.031	1.495HisSer: 1.495 ± 0.044	0.838HisThr: 0.838 ± 0.031	1.075HisVal: 1.075 ± 0.041	0.19HisTrp: 0.19 ± 0.014	0.791HisTyr: 0.791 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.768IleAla: 5.768 ± 0.108	1.165IleCys: 1.165 ± 0.04	4.481IleAsp: 4.481 ± 0.079	6.024IleGlu: 6.024 ± 0.088	5.261IlePhe: 5.261 ± 0.104	4.576IleGly: 4.576 ± 0.093	1.609IleHis: 1.609 ± 0.049	7.52IleIle: 7.52 ± 0.127	6.797IleLys: 6.797 ± 0.088	8.857IleLeu: 8.857 ± 0.121	1.467IleMet: 1.467 ± 0.042	5.678IleAsn: 5.678 ± 0.109	3.506IlePro: 3.506 ± 0.083	3.185IleGln: 3.185 ± 0.06	2.764IleArg: 2.764 ± 0.053	6.905IleSer: 6.905 ± 0.114	3.97IleThr: 3.97 ± 0.097	4.769IleVal: 4.769 ± 0.078	0.719IleTrp: 0.719 ± 0.034	3.034IleTyr: 3.034 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	3.776LysAla: 3.776 ± 0.08	0.699LysCys: 0.699 ± 0.031	3.733LysAsp: 3.733 ± 0.077	5.596LysGlu: 5.596 ± 0.086	3.966LysPhe: 3.966 ± 0.073	3.168LysGly: 3.168 ± 0.071	1.392LysHis: 1.392 ± 0.043	9.15LysIle: 9.15 ± 0.136	8.21LysLys: 8.21 ± 0.119	7.438LysLeu: 7.438 ± 0.094	1.827LysMet: 1.827 ± 0.051	8.183LysAsn: 8.183 ± 0.128	2.228LysPro: 2.228 ± 0.056	2.822LysGln: 2.822 ± 0.071	2.659LysArg: 2.659 ± 0.059	5.448LysSer: 5.448 ± 0.084	4.334LysThr: 4.334 ± 0.077	4.309LysVal: 4.309 ± 0.077	0.689LysTrp: 0.689 ± 0.03	3.053LysTyr: 3.053 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	6.259LeuAla: 6.259 ± 0.094	1.23LeuCys: 1.23 ± 0.04	4.581LeuAsp: 4.581 ± 0.073	6.332LeuGlu: 6.332 ± 0.095	5.488LeuPhe: 5.488 ± 0.108	5.509LeuGly: 5.509 ± 0.081	1.817LeuHis: 1.817 ± 0.048	7.847LeuIle: 7.847 ± 0.131	8.721LeuLys: 8.721 ± 0.115	9.394LeuLeu: 9.394 ± 0.124	1.845LeuMet: 1.845 ± 0.048	7.248LeuAsn: 7.248 ± 0.118	3.903LeuPro: 3.903 ± 0.083	3.765LeuGln: 3.765 ± 0.063	3.56LeuArg: 3.56 ± 0.074	8.415LeuSer: 8.415 ± 0.121	5.033LeuThr: 5.033 ± 0.095	4.968LeuVal: 4.968 ± 0.088	0.801LeuTrp: 0.801 ± 0.031	3.0LeuTyr: 3.0 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.25MetAla: 1.25 ± 0.044	0.238MetCys: 0.238 ± 0.017	0.803MetAsp: 0.803 ± 0.033	1.022MetGlu: 1.022 ± 0.037	0.865MetPhe: 0.865 ± 0.032	1.174MetGly: 1.174 ± 0.043	0.446MetHis: 0.446 ± 0.026	1.494MetIle: 1.494 ± 0.049	1.568MetLys: 1.568 ± 0.045	1.918MetLeu: 1.918 ± 0.054	0.465MetMet: 0.465 ± 0.025	1.154MetAsn: 1.154 ± 0.037	0.901MetPro: 0.901 ± 0.036	0.968MetGln: 0.968 ± 0.039	0.944MetArg: 0.944 ± 0.038	1.668MetSer: 1.668 ± 0.043	1.053MetThr: 1.053 ± 0.037	1.032MetVal: 1.032 ± 0.039	0.125MetTrp: 0.125 ± 0.014	0.522MetTyr: 0.522 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.487AsnAla: 3.487 ± 0.071	0.774AsnCys: 0.774 ± 0.035	2.829AsnAsp: 2.829 ± 0.062	3.862AsnGlu: 3.862 ± 0.085	4.205AsnPhe: 4.205 ± 0.102	2.894AsnGly: 2.894 ± 0.067	1.295AsnHis: 1.295 ± 0.039	6.401AsnIle: 6.401 ± 0.106	5.936AsnLys: 5.936 ± 0.109	7.004AsnLeu: 7.004 ± 0.145	1.281AsnMet: 1.281 ± 0.036	5.574AsnAsn: 5.574 ± 0.147	2.548AsnPro: 2.548 ± 0.056	2.497AsnGln: 2.497 ± 0.065	1.921AsnArg: 1.921 ± 0.046	5.532AsnSer: 5.532 ± 0.117	3.207AsnThr: 3.207 ± 0.082	2.969AsnVal: 2.969 ± 0.061	0.713AsnTrp: 0.713 ± 0.032	3.049AsnTyr: 3.049 ± 0.085	0.0AsnXaa: 0.0 ± 0.0
Pro	1.915ProAla: 1.915 ± 0.057	0.369ProCys: 0.369 ± 0.02	1.606ProAsp: 1.606 ± 0.048	2.636ProGlu: 2.636 ± 0.057	2.038ProPhe: 2.038 ± 0.051	1.76ProGly: 1.76 ± 0.051	0.779ProHis: 0.779 ± 0.033	2.928ProIle: 2.928 ± 0.055	2.707ProLys: 2.707 ± 0.061	3.273ProLeu: 3.273 ± 0.061	0.57ProMet: 0.57 ± 0.029	2.179ProAsn: 2.179 ± 0.057	1.142ProPro: 1.142 ± 0.045	1.415ProGln: 1.415 ± 0.038	1.051ProArg: 1.051 ± 0.042	2.547ProSer: 2.547 ± 0.067	1.798ProThr: 1.798 ± 0.061	2.048ProVal: 2.048 ± 0.055	0.352ProTrp: 0.352 ± 0.018	1.201ProTyr: 1.201 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.078GlnAla: 2.078 ± 0.051	0.358GlnCys: 0.358 ± 0.021	1.667GlnAsp: 1.667 ± 0.043	2.684GlnGlu: 2.684 ± 0.053	1.85GlnPhe: 1.85 ± 0.052	1.712GlnGly: 1.712 ± 0.049	0.688GlnHis: 0.688 ± 0.032	3.258GlnIle: 3.258 ± 0.06	3.586GlnLys: 3.586 ± 0.065	3.34GlnLeu: 3.34 ± 0.065	0.724GlnMet: 0.724 ± 0.03	3.108GlnAsn: 3.108 ± 0.063	1.009GlnPro: 1.009 ± 0.034	1.538GlnGln: 1.538 ± 0.056	1.35GlnArg: 1.35 ± 0.048	2.572GlnSer: 2.572 ± 0.063	1.912GlnThr: 1.912 ± 0.053	1.866GlnVal: 1.866 ± 0.053	0.322GlnTrp: 0.322 ± 0.02	1.131GlnTyr: 1.131 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.26ArgAla: 2.26 ± 0.052	0.406ArgCys: 0.406 ± 0.022	1.659ArgAsp: 1.659 ± 0.052	2.295ArgGlu: 2.295 ± 0.06	1.883ArgPhe: 1.883 ± 0.056	1.8ArgGly: 1.8 ± 0.051	0.643ArgHis: 0.643 ± 0.031	3.146ArgIle: 3.146 ± 0.067	2.746ArgLys: 2.746 ± 0.063	3.364ArgLeu: 3.364 ± 0.07	0.844ArgMet: 0.844 ± 0.034	2.178ArgAsn: 2.178 ± 0.045	1.121ArgPro: 1.121 ± 0.041	1.13ArgGln: 1.13 ± 0.041	1.534ArgArg: 1.534 ± 0.051	2.486ArgSer: 2.486 ± 0.057	1.634ArgThr: 1.634 ± 0.048	2.237ArgVal: 2.237 ± 0.064	0.346ArgTrp: 0.346 ± 0.02	1.303ArgTyr: 1.303 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	4.309SerAla: 4.309 ± 0.093	0.912SerCys: 0.912 ± 0.035	3.379SerAsp: 3.379 ± 0.066	4.767SerGlu: 4.767 ± 0.085	4.353SerPhe: 4.353 ± 0.077	4.471SerGly: 4.471 ± 0.077	1.546SerHis: 1.546 ± 0.04	6.564SerIle: 6.564 ± 0.103	5.648SerLys: 5.648 ± 0.093	8.431SerLeu: 8.431 ± 0.102	1.495SerMet: 1.495 ± 0.044	4.505SerAsn: 4.505 ± 0.099	2.482SerPro: 2.482 ± 0.058	3.108SerGln: 3.108 ± 0.064	2.639SerArg: 2.639 ± 0.058	6.0SerSer: 6.0 ± 0.12	3.252SerThr: 3.252 ± 0.085	4.175SerVal: 4.175 ± 0.083	0.769SerTrp: 0.769 ± 0.037	2.695SerTyr: 2.695 ± 0.074	0.0SerXaa: 0.0 ± 0.0
Thr	3.173ThrAla: 3.173 ± 0.084	0.573ThrCys: 0.573 ± 0.031	2.053ThrAsp: 2.053 ± 0.058	2.998ThrGlu: 2.998 ± 0.062	2.403ThrPhe: 2.403 ± 0.052	3.061ThrGly: 3.061 ± 0.073	1.046ThrHis: 1.046 ± 0.035	4.052ThrIle: 4.052 ± 0.084	3.636ThrLys: 3.636 ± 0.078	5.053ThrLeu: 5.053 ± 0.087	0.912ThrMet: 0.912 ± 0.032	2.942ThrAsn: 2.942 ± 0.07	2.2ThrPro: 2.2 ± 0.059	2.01ThrGln: 2.01 ± 0.049	1.742ThrArg: 1.742 ± 0.048	3.702ThrSer: 3.702 ± 0.092	2.774ThrThr: 2.774 ± 0.069	2.792ThrVal: 2.792 ± 0.072	0.525ThrTrp: 0.525 ± 0.029	1.582ThrTyr: 1.582 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	3.959ValAla: 3.959 ± 0.091	0.762ValCys: 0.762 ± 0.031	2.699ValAsp: 2.699 ± 0.053	3.25ValGlu: 3.25 ± 0.073	3.248ValPhe: 3.248 ± 0.068	3.288ValGly: 3.288 ± 0.075	1.048ValHis: 1.048 ± 0.033	4.363ValIle: 4.363 ± 0.09	3.876ValLys: 3.876 ± 0.063	5.435ValLeu: 5.435 ± 0.083	1.032ValMet: 1.032 ± 0.038	3.051ValAsn: 3.051 ± 0.071	2.072ValPro: 2.072 ± 0.047	1.853ValGln: 1.853 ± 0.048	2.042ValArg: 2.042 ± 0.064	4.346ValSer: 4.346 ± 0.084	3.255ValThr: 3.255 ± 0.079	3.582ValVal: 3.582 ± 0.077	0.479ValTrp: 0.479 ± 0.026	1.882ValTyr: 1.882 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.457TrpAla: 0.457 ± 0.029	0.144TrpCys: 0.144 ± 0.013	0.471TrpAsp: 0.471 ± 0.026	0.53TrpGlu: 0.53 ± 0.026	0.511TrpPhe: 0.511 ± 0.027	0.529TrpGly: 0.529 ± 0.03	0.209TrpHis: 0.209 ± 0.016	0.802TrpIle: 0.802 ± 0.03	0.724TrpLys: 0.724 ± 0.028	1.144TrpLeu: 1.144 ± 0.043	0.215TrpMet: 0.215 ± 0.016	0.613TrpAsn: 0.613 ± 0.024	0.324TrpPro: 0.324 ± 0.019	0.438TrpGln: 0.438 ± 0.022	0.383TrpArg: 0.383 ± 0.02	0.615TrpSer: 0.615 ± 0.029	0.396TrpThr: 0.396 ± 0.022	0.523TrpVal: 0.523 ± 0.024	0.124TrpTrp: 0.124 ± 0.013	0.294TrpTyr: 0.294 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.976TyrAla: 1.976 ± 0.053	0.58TyrCys: 0.58 ± 0.029	1.695TyrAsp: 1.695 ± 0.052	2.024TyrGlu: 2.024 ± 0.058	2.564TyrPhe: 2.564 ± 0.067	1.913TyrGly: 1.913 ± 0.05	0.685TyrHis: 0.685 ± 0.03	2.662TyrIle: 2.662 ± 0.058	2.619TyrLys: 2.619 ± 0.065	3.817TyrLeu: 3.817 ± 0.093	0.546TyrMet: 0.546 ± 0.025	2.219TyrAsn: 2.219 ± 0.069	1.304TyrPro: 1.304 ± 0.038	1.388TyrGln: 1.388 ± 0.046	1.273TyrArg: 1.273 ± 0.044	2.888TyrSer: 2.888 ± 0.07	1.65TyrThr: 1.65 ± 0.049	1.826TyrVal: 1.826 ± 0.043	0.397TyrTrp: 0.397 ± 0.022	1.555TyrTyr: 1.555 ± 0.066	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2415 proteins (824229 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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