Erwinia phage phiEaP-8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Erskinevirinae; Johnsonvirus; unclassified Johnsonvirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1QTM3|A0A3G1QTM3_9CAUD DNA primase OS=Erwinia phage phiEaP-8 OX=2178928 PE=4 SV=1
MM1 pKa = 8.22DD2 pKa = 3.83EE3 pKa = 4.57HH4 pKa = 5.86QQQVLRR10 pKa = 11.84DD11 pKa = 3.97FLAEE15 pKa = 3.86YY16 pKa = 8.14WDD18 pKa = 3.56RR19 pKa = 11.84WEE21 pKa = 3.87EE22 pKa = 3.83HH23 pKa = 6.51AFQNNEE29 pKa = 3.94SGQEE33 pKa = 3.79IYY35 pKa = 10.67EE36 pKa = 4.14LLGGEE41 pKa = 4.22PDD43 pKa = 3.79GNN45 pKa = 3.62

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1QTR7|A0A3G1QTR7_9CAUD Uncharacterized protein OS=Erwinia phage phiEaP-8 OX=2178928 PE=4 SV=1
MM1 pKa = 7.43LLSRR5 pKa = 11.84SYY7 pKa = 10.75AICEE11 pKa = 3.84ICGEE15 pKa = 4.06PRR17 pKa = 11.84SKK19 pKa = 10.24RR20 pKa = 11.84VHH22 pKa = 6.1RR23 pKa = 11.84KK24 pKa = 9.29CSRR27 pKa = 11.84EE28 pKa = 3.62LQRR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 11.16DD34 pKa = 3.51PVLNAKK40 pKa = 9.75INAGVRR46 pKa = 11.84ADD48 pKa = 3.65EE49 pKa = 5.13LKK51 pKa = 10.65ISMQRR56 pKa = 11.84EE57 pKa = 3.51LSVRR61 pKa = 11.84IKK63 pKa = 11.01GRR65 pKa = 11.84TKK67 pKa = 10.18EE68 pKa = 3.87

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

23962

45

3508

307.2

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.219 ± 0.525

0.755 ± 0.146

5.989 ± 0.134

6.118 ± 0.315

3.506 ± 0.215

6.907 ± 0.312

1.736 ± 0.161

5.471 ± 0.238

5.826 ± 0.306

8.0 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.984 ± 0.16

5.751 ± 0.317

4.132 ± 0.164

4.866 ± 0.364

4.336 ± 0.265

6.339 ± 0.286

6.51 ± 0.352

7.003 ± 0.276

1.285 ± 0.178

3.268 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski