Amino acid dipepetide frequency for Bacillus megaterium (strain ATCC 12872 / QMB1551)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.276AlaAla: 6.276 ± 0.069	0.669AlaCys: 0.669 ± 0.022	3.329AlaAsp: 3.329 ± 0.058	4.459AlaGlu: 4.459 ± 0.068	3.525AlaPhe: 3.525 ± 0.054	5.059AlaGly: 5.059 ± 0.066	1.45AlaHis: 1.45 ± 0.032	5.625AlaIle: 5.625 ± 0.064	4.912AlaLys: 4.912 ± 0.059	7.529AlaLeu: 7.529 ± 0.073	1.95AlaMet: 1.95 ± 0.038	2.762AlaAsn: 2.762 ± 0.045	2.15AlaPro: 2.15 ± 0.041	2.475AlaGln: 2.475 ± 0.054	2.475AlaArg: 2.475 ± 0.041	4.559AlaSer: 4.559 ± 0.054	3.616AlaThr: 3.616 ± 0.072	5.791AlaVal: 5.791 ± 0.069	0.608AlaTrp: 0.608 ± 0.021	2.463AlaTyr: 2.463 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.466CysAla: 0.466 ± 0.017	0.122CysCys: 0.122 ± 0.009	0.356CysAsp: 0.356 ± 0.017	0.473CysGlu: 0.473 ± 0.017	0.38CysPhe: 0.38 ± 0.014	0.679CysGly: 0.679 ± 0.023	0.199CysHis: 0.199 ± 0.01	0.576CysIle: 0.576 ± 0.018	0.411CysLys: 0.411 ± 0.018	0.782CysLeu: 0.782 ± 0.02	0.217CysMet: 0.217 ± 0.012	0.273CysAsn: 0.273 ± 0.015	0.326CysPro: 0.326 ± 0.016	0.237CysGln: 0.237 ± 0.014	0.277CysArg: 0.277 ± 0.014	0.61CysSer: 0.61 ± 0.019	0.425CysThr: 0.425 ± 0.016	0.469CysVal: 0.469 ± 0.019	0.084CysTrp: 0.084 ± 0.007	0.302CysTyr: 0.302 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.189AspAla: 3.189 ± 0.047	0.356AspCys: 0.356 ± 0.015	2.163AspAsp: 2.163 ± 0.043	4.021AspGlu: 4.021 ± 0.059	2.214AspPhe: 2.214 ± 0.038	2.881AspGly: 2.881 ± 0.048	1.124AspHis: 1.124 ± 0.033	3.955AspIle: 3.955 ± 0.056	3.033AspLys: 3.033 ± 0.054	4.665AspLeu: 4.665 ± 0.06	1.256AspMet: 1.256 ± 0.031	1.608AspAsn: 1.608 ± 0.035	1.716AspPro: 1.716 ± 0.04	1.913AspGln: 1.913 ± 0.038	1.923AspArg: 1.923 ± 0.034	2.601AspSer: 2.601 ± 0.046	2.323AspThr: 2.323 ± 0.039	3.92AspVal: 3.92 ± 0.048	0.544AspTrp: 0.544 ± 0.02	2.002AspTyr: 2.002 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	5.401GluAla: 5.401 ± 0.077	0.431GluCys: 0.431 ± 0.02	3.412GluAsp: 3.412 ± 0.054	6.719GluGlu: 6.719 ± 0.097	2.422GluPhe: 2.422 ± 0.041	4.013GluGly: 4.013 ± 0.057	1.615GluHis: 1.615 ± 0.031	4.831GluIle: 4.831 ± 0.068	6.64GluLys: 6.64 ± 0.076	6.974GluLeu: 6.974 ± 0.083	2.077GluMet: 2.077 ± 0.038	3.307GluAsn: 3.307 ± 0.048	1.78GluPro: 1.78 ± 0.034	3.498GluGln: 3.498 ± 0.065	3.37GluArg: 3.37 ± 0.048	3.328GluSer: 3.328 ± 0.051	3.708GluThr: 3.708 ± 0.062	4.985GluVal: 4.985 ± 0.065	0.753GluTrp: 0.753 ± 0.024	2.169GluTyr: 2.169 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.023PheAla: 3.023 ± 0.053	0.366PheCys: 0.366 ± 0.015	2.194PheAsp: 2.194 ± 0.041	2.663PheGlu: 2.663 ± 0.04	2.514PhePhe: 2.514 ± 0.052	3.201PheGly: 3.201 ± 0.044	1.025PheHis: 1.025 ± 0.03	3.985PheIle: 3.985 ± 0.065	2.49PheLys: 2.49 ± 0.045	4.779PheLeu: 4.779 ± 0.064	1.211PheMet: 1.211 ± 0.028	1.769PheAsn: 1.769 ± 0.035	1.661PhePro: 1.661 ± 0.035	1.719PheGln: 1.719 ± 0.035	1.405PheArg: 1.405 ± 0.034	3.494PheSer: 3.494 ± 0.054	2.647PheThr: 2.647 ± 0.04	3.301PheVal: 3.301 ± 0.053	0.494PheTrp: 0.494 ± 0.019	1.786PheTyr: 1.786 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	4.941GlyAla: 4.941 ± 0.08	0.642GlyCys: 0.642 ± 0.02	2.967GlyAsp: 2.967 ± 0.043	4.146GlyGlu: 4.146 ± 0.058	3.387GlyPhe: 3.387 ± 0.054	4.796GlyGly: 4.796 ± 0.083	1.327GlyHis: 1.327 ± 0.031	5.881GlyIle: 5.881 ± 0.083	4.832GlyLys: 4.832 ± 0.059	6.392GlyLeu: 6.392 ± 0.078	2.07GlyMet: 2.07 ± 0.043	2.569GlyAsn: 2.569 ± 0.046	1.671GlyPro: 1.671 ± 0.036	2.196GlyGln: 2.196 ± 0.034	2.318GlyArg: 2.318 ± 0.043	3.976GlySer: 3.976 ± 0.056	3.902GlyThr: 3.902 ± 0.055	5.276GlyVal: 5.276 ± 0.077	0.796GlyTrp: 0.796 ± 0.025	2.766GlyTyr: 2.766 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	1.403HisAla: 1.403 ± 0.03	0.195HisCys: 0.195 ± 0.013	1.054HisAsp: 1.054 ± 0.03	1.541HisGlu: 1.541 ± 0.035	1.082HisPhe: 1.082 ± 0.032	1.367HisGly: 1.367 ± 0.03	0.783HisHis: 0.783 ± 0.028	1.783HisIle: 1.783 ± 0.036	1.226HisLys: 1.226 ± 0.031	2.308HisLeu: 2.308 ± 0.039	0.588HisMet: 0.588 ± 0.021	0.802HisAsn: 0.802 ± 0.024	1.193HisPro: 1.193 ± 0.031	0.951HisGln: 0.951 ± 0.027	0.904HisArg: 0.904 ± 0.025	1.404HisSer: 1.404 ± 0.032	1.254HisThr: 1.254 ± 0.032	1.651HisVal: 1.651 ± 0.036	0.234HisTrp: 0.234 ± 0.012	0.973HisTyr: 0.973 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.814IleAla: 5.814 ± 0.07	0.693IleCys: 0.693 ± 0.022	3.977IleAsp: 3.977 ± 0.047	5.34IleGlu: 5.34 ± 0.07	3.181IlePhe: 3.181 ± 0.063	5.954IleGly: 5.954 ± 0.077	1.738IleHis: 1.738 ± 0.034	5.552IleIle: 5.552 ± 0.087	4.628IleLys: 4.628 ± 0.058	6.862IleLeu: 6.862 ± 0.083	1.783IleMet: 1.783 ± 0.033	3.075IleAsn: 3.075 ± 0.051	3.155IlePro: 3.155 ± 0.042	3.002IleGln: 3.002 ± 0.042	2.714IleArg: 2.714 ± 0.044	5.155IleSer: 5.155 ± 0.062	4.174IleThr: 4.174 ± 0.064	5.652IleVal: 5.652 ± 0.064	0.642IleTrp: 0.642 ± 0.023	2.416IleTyr: 2.416 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.987LysAla: 4.987 ± 0.063	0.322LysCys: 0.322 ± 0.015	3.834LysAsp: 3.834 ± 0.06	7.277LysGlu: 7.277 ± 0.082	1.893LysPhe: 1.893 ± 0.038	4.777LysGly: 4.777 ± 0.059	1.519LysHis: 1.519 ± 0.034	4.356LysIle: 4.356 ± 0.059	6.605LysLys: 6.605 ± 0.084	5.72LysLeu: 5.72 ± 0.06	2.241LysMet: 2.241 ± 0.041	3.299LysAsn: 3.299 ± 0.054	2.244LysPro: 2.244 ± 0.043	3.805LysGln: 3.805 ± 0.059	3.468LysArg: 3.468 ± 0.053	3.704LysSer: 3.704 ± 0.049	3.76LysThr: 3.76 ± 0.056	4.8LysVal: 4.8 ± 0.065	0.9LysTrp: 0.9 ± 0.028	2.088LysTyr: 2.088 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	7.335LeuAla: 7.335 ± 0.079	0.836LeuCys: 0.836 ± 0.024	4.388LeuAsp: 4.388 ± 0.057	5.998LeuGlu: 5.998 ± 0.081	5.195LeuPhe: 5.195 ± 0.068	6.391LeuGly: 6.391 ± 0.078	2.276LeuHis: 2.276 ± 0.041	7.169LeuIle: 7.169 ± 0.089	6.776LeuLys: 6.776 ± 0.07	10.581LeuLeu: 10.581 ± 0.116	2.501LeuMet: 2.501 ± 0.044	4.232LeuAsn: 4.232 ± 0.05	3.887LeuPro: 3.887 ± 0.055	3.888LeuGln: 3.888 ± 0.059	3.36LeuArg: 3.36 ± 0.055	7.23LeuSer: 7.23 ± 0.078	5.999LeuThr: 5.999 ± 0.068	6.515LeuVal: 6.515 ± 0.082	0.845LeuTrp: 0.845 ± 0.027	3.273LeuTyr: 3.273 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.867MetAla: 1.867 ± 0.038	0.149MetCys: 0.149 ± 0.01	1.238MetAsp: 1.238 ± 0.027	1.691MetGlu: 1.691 ± 0.037	1.102MetPhe: 1.102 ± 0.029	1.669MetGly: 1.669 ± 0.033	0.493MetHis: 0.493 ± 0.022	2.151MetIle: 2.151 ± 0.042	2.674MetLys: 2.674 ± 0.041	2.615MetLeu: 2.615 ± 0.038	0.921MetMet: 0.921 ± 0.027	1.65MetAsn: 1.65 ± 0.036	0.989MetPro: 0.989 ± 0.031	0.935MetGln: 0.935 ± 0.029	1.007MetArg: 1.007 ± 0.025	1.872MetSer: 1.872 ± 0.039	1.603MetThr: 1.603 ± 0.032	1.712MetVal: 1.712 ± 0.037	0.231MetTrp: 0.231 ± 0.012	0.816MetTyr: 0.816 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.684AsnAla: 2.684 ± 0.048	0.298AsnCys: 0.298 ± 0.014	2.06AsnAsp: 2.06 ± 0.045	3.44AsnGlu: 3.44 ± 0.054	1.462AsnPhe: 1.462 ± 0.033	3.015AsnGly: 3.015 ± 0.051	1.075AsnHis: 1.075 ± 0.027	3.171AsnIle: 3.171 ± 0.05	2.98AsnLys: 2.98 ± 0.05	3.689AsnLeu: 3.689 ± 0.047	1.155AsnMet: 1.155 ± 0.029	1.832AsnAsn: 1.832 ± 0.041	1.947AsnPro: 1.947 ± 0.036	2.006AsnGln: 2.006 ± 0.039	1.916AsnArg: 1.916 ± 0.032	2.338AsnSer: 2.338 ± 0.047	2.087AsnThr: 2.087 ± 0.041	3.196AsnVal: 3.196 ± 0.047	0.508AsnTrp: 0.508 ± 0.019	1.48AsnTyr: 1.48 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	2.434ProAla: 2.434 ± 0.041	0.203ProCys: 0.203 ± 0.011	1.749ProAsp: 1.749 ± 0.036	2.513ProGlu: 2.513 ± 0.046	2.075ProPhe: 2.075 ± 0.039	2.091ProGly: 2.091 ± 0.042	0.88ProHis: 0.88 ± 0.027	2.591ProIle: 2.591 ± 0.039	2.202ProLys: 2.202 ± 0.04	3.668ProLeu: 3.668 ± 0.056	0.776ProMet: 0.776 ± 0.03	1.58ProAsn: 1.58 ± 0.035	0.993ProPro: 0.993 ± 0.031	1.23ProGln: 1.23 ± 0.032	1.077ProArg: 1.077 ± 0.031	2.483ProSer: 2.483 ± 0.045	1.965ProThr: 1.965 ± 0.036	2.821ProVal: 2.821 ± 0.046	0.382ProTrp: 0.382 ± 0.017	1.494ProTyr: 1.494 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.067GlnAla: 3.067 ± 0.057	0.229GlnCys: 0.229 ± 0.014	1.707GlnAsp: 1.707 ± 0.031	2.967GlnGlu: 2.967 ± 0.05	1.658GlnPhe: 1.658 ± 0.037	2.373GlnGly: 2.373 ± 0.045	1.065GlnHis: 1.065 ± 0.028	2.444GlnIle: 2.444 ± 0.043	3.217GlnLys: 3.217 ± 0.049	4.262GlnLeu: 4.262 ± 0.053	1.117GlnMet: 1.117 ± 0.028	1.645GlnAsn: 1.645 ± 0.035	1.332GlnPro: 1.332 ± 0.03	2.289GlnGln: 2.289 ± 0.058	1.621GlnArg: 1.621 ± 0.032	2.349GlnSer: 2.349 ± 0.037	2.309GlnThr: 2.309 ± 0.045	2.524GlnVal: 2.524 ± 0.041	0.409GlnTrp: 0.409 ± 0.016	1.469GlnTyr: 1.469 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.304ArgAla: 2.304 ± 0.042	0.249ArgCys: 0.249 ± 0.011	1.901ArgAsp: 1.901 ± 0.038	2.928ArgGlu: 2.928 ± 0.049	1.806ArgPhe: 1.806 ± 0.037	2.099ArgGly: 2.099 ± 0.041	0.829ArgHis: 0.829 ± 0.025	2.865ArgIle: 2.865 ± 0.045	3.079ArgLys: 3.079 ± 0.043	3.733ArgLeu: 3.733 ± 0.053	1.19ArgMet: 1.19 ± 0.025	1.711ArgAsn: 1.711 ± 0.033	1.226ArgPro: 1.226 ± 0.031	1.492ArgGln: 1.492 ± 0.035	1.71ArgArg: 1.71 ± 0.049	2.273ArgSer: 2.273 ± 0.047	2.078ArgThr: 2.078 ± 0.044	2.533ArgVal: 2.533 ± 0.044	0.364ArgTrp: 0.364 ± 0.015	1.599ArgTyr: 1.599 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	4.185SerAla: 4.185 ± 0.048	0.483SerCys: 0.483 ± 0.017	2.694SerAsp: 2.694 ± 0.049	3.779SerGlu: 3.779 ± 0.047	3.522SerPhe: 3.522 ± 0.047	4.289SerGly: 4.289 ± 0.055	1.41SerHis: 1.41 ± 0.033	5.106SerIle: 5.106 ± 0.067	4.147SerLys: 4.147 ± 0.056	6.865SerLeu: 6.865 ± 0.071	1.851SerMet: 1.851 ± 0.036	2.539SerAsn: 2.539 ± 0.041	2.288SerPro: 2.288 ± 0.041	2.324SerGln: 2.324 ± 0.044	2.149SerArg: 2.149 ± 0.04	4.729SerSer: 4.729 ± 0.072	3.534SerThr: 3.534 ± 0.053	4.471SerVal: 4.471 ± 0.054	0.652SerTrp: 0.652 ± 0.021	2.525SerTyr: 2.525 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.1ThrAla: 4.1 ± 0.054	0.414ThrCys: 0.414 ± 0.019	2.523ThrAsp: 2.523 ± 0.044	3.338ThrGlu: 3.338 ± 0.055	2.79ThrPhe: 2.79 ± 0.042	4.196ThrGly: 4.196 ± 0.163	1.182ThrHis: 1.182 ± 0.028	4.377ThrIle: 4.377 ± 0.058	3.604ThrLys: 3.604 ± 0.052	5.573ThrLeu: 5.573 ± 0.065	1.279ThrMet: 1.279 ± 0.027	2.486ThrAsn: 2.486 ± 0.038	2.323ThrPro: 2.323 ± 0.045	1.57ThrGln: 1.57 ± 0.032	1.786ThrArg: 1.786 ± 0.04	3.604ThrSer: 3.604 ± 0.051	2.916ThrThr: 2.916 ± 0.051	4.409ThrVal: 4.409 ± 0.063	0.559ThrTrp: 0.559 ± 0.02	2.188ThrTyr: 2.188 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	5.279ValAla: 5.279 ± 0.066	0.624ValCys: 0.624 ± 0.019	3.514ValAsp: 3.514 ± 0.053	4.856ValGlu: 4.856 ± 0.069	3.173ValPhe: 3.173 ± 0.052	4.805ValGly: 4.805 ± 0.066	1.564ValHis: 1.564 ± 0.035	5.683ValIle: 5.683 ± 0.067	5.09ValLys: 5.09 ± 0.071	7.134ValLeu: 7.134 ± 0.09	1.951ValMet: 1.951 ± 0.04	3.169ValAsn: 3.169 ± 0.045	2.728ValPro: 2.728 ± 0.04	2.657ValGln: 2.657 ± 0.041	2.54ValArg: 2.54 ± 0.04	4.988ValSer: 4.988 ± 0.055	4.36ValThr: 4.36 ± 0.087	5.417ValVal: 5.417 ± 0.075	0.65ValTrp: 0.65 ± 0.02	2.504ValTyr: 2.504 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.627TrpAla: 0.627 ± 0.019	0.078TrpCys: 0.078 ± 0.008	0.457TrpAsp: 0.457 ± 0.018	0.614TrpGlu: 0.614 ± 0.02	0.572TrpPhe: 0.572 ± 0.021	0.672TrpGly: 0.672 ± 0.023	0.232TrpHis: 0.232 ± 0.014	0.836TrpIle: 0.836 ± 0.026	0.809TrpLys: 0.809 ± 0.027	1.143TrpLeu: 1.143 ± 0.029	0.34TrpMet: 0.34 ± 0.015	0.588TrpAsn: 0.588 ± 0.022	0.249TrpPro: 0.249 ± 0.014	0.312TrpGln: 0.312 ± 0.013	0.374TrpArg: 0.374 ± 0.018	0.673TrpSer: 0.673 ± 0.019	0.52TrpThr: 0.52 ± 0.018	0.661TrpVal: 0.661 ± 0.024	0.153TrpTrp: 0.153 ± 0.01	0.32TrpTyr: 0.32 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.3TyrAla: 2.3 ± 0.039	0.307TyrCys: 0.307 ± 0.014	1.889TyrAsp: 1.889 ± 0.037	2.669TyrGlu: 2.669 ± 0.05	1.774TyrPhe: 1.774 ± 0.038	2.527TyrGly: 2.527 ± 0.045	0.909TyrHis: 0.909 ± 0.025	2.571TyrIle: 2.571 ± 0.047	2.244TyrLys: 2.244 ± 0.037	3.4TyrLeu: 3.4 ± 0.057	0.912TyrMet: 0.912 ± 0.022	1.445TyrAsn: 1.445 ± 0.033	1.348TyrPro: 1.348 ± 0.03	1.554TyrGln: 1.554 ± 0.033	1.575TyrArg: 1.575 ± 0.031	2.173TyrSer: 2.173 ± 0.037	2.036TyrThr: 2.036 ± 0.035	2.581TyrVal: 2.581 ± 0.045	0.422TyrTrp: 0.422 ± 0.015	1.498TyrTyr: 1.498 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5606 proteins (1495755 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---