Amino acid dipepetide frequency for Synechococcus sp. WH 5701

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.579AlaAla: 15.579 ± 0.175	1.438AlaCys: 1.438 ± 0.041	5.688AlaAsp: 5.688 ± 0.07	7.272AlaGlu: 7.272 ± 0.107	3.213AlaPhe: 3.213 ± 0.061	10.181AlaGly: 10.181 ± 0.12	1.842AlaHis: 1.842 ± 0.042	4.921AlaIle: 4.921 ± 0.073	2.279AlaLys: 2.279 ± 0.063	15.382AlaLeu: 15.382 ± 0.184	2.862AlaMet: 2.862 ± 0.051	2.266AlaAsn: 2.266 ± 0.055	5.987AlaPro: 5.987 ± 0.087	4.234AlaGln: 4.234 ± 0.065	7.491AlaArg: 7.491 ± 0.097	7.059AlaSer: 7.059 ± 0.099	4.929AlaThr: 4.929 ± 0.081	8.403AlaVal: 8.403 ± 0.109	1.808AlaTrp: 1.808 ± 0.055	1.942AlaTyr: 1.942 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.989CysAla: 0.989 ± 0.032	0.246CysCys: 0.246 ± 0.018	0.576CysAsp: 0.576 ± 0.027	0.549CysGlu: 0.549 ± 0.026	0.385CysPhe: 0.385 ± 0.019	1.198CysGly: 1.198 ± 0.039	0.349CysHis: 0.349 ± 0.022	0.345CysIle: 0.345 ± 0.023	0.17CysLys: 0.17 ± 0.011	1.339CysLeu: 1.339 ± 0.045	0.196CysMet: 0.196 ± 0.015	0.269CysAsn: 0.269 ± 0.016	0.736CysPro: 0.736 ± 0.027	0.438CysGln: 0.438 ± 0.025	1.118CysArg: 1.118 ± 0.039	0.859CysSer: 0.859 ± 0.033	0.471CysThr: 0.471 ± 0.02	0.656CysVal: 0.656 ± 0.026	0.275CysTrp: 0.275 ± 0.017	0.18CysTyr: 0.18 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.686AspAla: 4.686 ± 0.074	0.484AspCys: 0.484 ± 0.022	2.447AspAsp: 2.447 ± 0.058	2.404AspGlu: 2.404 ± 0.053	1.44AspPhe: 1.44 ± 0.049	4.804AspGly: 4.804 ± 0.083	1.195AspHis: 1.195 ± 0.037	1.463AspIle: 1.463 ± 0.045	0.873AspLys: 0.873 ± 0.039	7.054AspLeu: 7.054 ± 0.098	0.647AspMet: 0.647 ± 0.027	0.878AspAsn: 0.878 ± 0.034	4.43AspPro: 4.43 ± 0.075	2.551AspGln: 2.551 ± 0.057	3.756AspArg: 3.756 ± 0.061	2.352AspSer: 2.352 ± 0.053	1.971AspThr: 1.971 ± 0.051	2.826AspVal: 2.826 ± 0.062	0.978AspTrp: 0.978 ± 0.036	1.022AspTyr: 1.022 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.375GluAla: 8.375 ± 0.102	0.405GluCys: 0.405 ± 0.022	2.188GluAsp: 2.188 ± 0.056	3.35GluGlu: 3.35 ± 0.08	1.376GluPhe: 1.376 ± 0.041	4.768GluGly: 4.768 ± 0.065	1.225GluHis: 1.225 ± 0.034	2.232GluIle: 2.232 ± 0.057	1.354GluLys: 1.354 ± 0.05	8.331GluLeu: 8.331 ± 0.103	0.977GluMet: 0.977 ± 0.032	1.016GluAsn: 1.016 ± 0.036	3.82GluPro: 3.82 ± 0.079	3.471GluGln: 3.471 ± 0.073	6.022GluArg: 6.022 ± 0.1	2.83GluSer: 2.83 ± 0.059	2.361GluThr: 2.361 ± 0.055	3.877GluVal: 3.877 ± 0.073	0.767GluTrp: 0.767 ± 0.03	0.598GluTyr: 0.598 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.006PheAla: 3.006 ± 0.066	0.458PheCys: 0.458 ± 0.024	1.943PheAsp: 1.943 ± 0.044	1.838PheGlu: 1.838 ± 0.048	1.064PhePhe: 1.064 ± 0.037	2.85PheGly: 2.85 ± 0.065	0.696PheHis: 0.696 ± 0.028	1.11PheIle: 1.11 ± 0.04	0.636PheLys: 0.636 ± 0.029	3.396PheLeu: 3.396 ± 0.072	0.498PheMet: 0.498 ± 0.026	0.997PheAsn: 0.997 ± 0.035	1.493PhePro: 1.493 ± 0.038	1.192PheGln: 1.192 ± 0.037	2.206PheArg: 2.206 ± 0.054	2.379PheSer: 2.379 ± 0.065	1.575PheThr: 1.575 ± 0.041	1.819PheVal: 1.819 ± 0.049	0.581PheTrp: 0.581 ± 0.028	0.712PheTyr: 0.712 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	8.466GlyAla: 8.466 ± 0.107	1.269GlyCys: 1.269 ± 0.031	4.321GlyAsp: 4.321 ± 0.069	5.003GlyGlu: 5.003 ± 0.073	3.293GlyPhe: 3.293 ± 0.06	7.587GlyGly: 7.587 ± 0.122	1.921GlyHis: 1.921 ± 0.047	3.675GlyIle: 3.675 ± 0.072	2.129GlyLys: 2.129 ± 0.051	11.85GlyLeu: 11.85 ± 0.157	1.968GlyMet: 1.968 ± 0.053	1.82GlyAsn: 1.82 ± 0.053	4.179GlyPro: 4.179 ± 0.068	3.482GlyGln: 3.482 ± 0.067	6.599GlyArg: 6.599 ± 0.082	6.009GlySer: 6.009 ± 0.087	4.029GlyThr: 4.029 ± 0.065	6.24GlyVal: 6.24 ± 0.08	1.979GlyTrp: 1.979 ± 0.05	1.922GlyTyr: 1.922 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.665HisAla: 1.665 ± 0.044	0.371HisCys: 0.371 ± 0.024	1.057HisAsp: 1.057 ± 0.033	0.837HisGlu: 0.837 ± 0.029	0.69HisPhe: 0.69 ± 0.029	2.155HisGly: 2.155 ± 0.055	0.809HisHis: 0.809 ± 0.036	0.593HisIle: 0.593 ± 0.028	0.34HisLys: 0.34 ± 0.018	2.574HisLeu: 2.574 ± 0.059	0.265HisMet: 0.265 ± 0.016	0.461HisAsn: 0.461 ± 0.024	1.752HisPro: 1.752 ± 0.043	1.142HisGln: 1.142 ± 0.035	1.819HisArg: 1.819 ± 0.045	1.153HisSer: 1.153 ± 0.036	0.831HisThr: 0.831 ± 0.036	1.02HisVal: 1.02 ± 0.04	0.63HisTrp: 0.63 ± 0.025	0.498HisTyr: 0.498 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	4.605IleAla: 4.605 ± 0.079	0.484IleCys: 0.484 ± 0.024	2.56IleAsp: 2.56 ± 0.054	2.835IleGlu: 2.835 ± 0.058	1.05IlePhe: 1.05 ± 0.04	3.89IleGly: 3.89 ± 0.072	0.912IleHis: 0.912 ± 0.035	1.015IleIle: 1.015 ± 0.039	1.037IleLys: 1.037 ± 0.034	3.46IleLeu: 3.46 ± 0.072	0.371IleMet: 0.371 ± 0.02	1.18IleAsn: 1.18 ± 0.036	2.492IlePro: 2.492 ± 0.062	1.523IleGln: 1.523 ± 0.039	2.875IleArg: 2.875 ± 0.064	2.746IleSer: 2.746 ± 0.052	2.464IleThr: 2.464 ± 0.049	2.39IleVal: 2.39 ± 0.06	0.57IleTrp: 0.57 ± 0.024	0.79IleTyr: 0.79 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	2.839LysAla: 2.839 ± 0.066	0.124LysCys: 0.124 ± 0.013	1.053LysAsp: 1.053 ± 0.04	1.214LysGlu: 1.214 ± 0.046	0.474LysPhe: 0.474 ± 0.027	1.984LysGly: 1.984 ± 0.059	0.317LysHis: 0.317 ± 0.02	0.819LysIle: 0.819 ± 0.034	0.661LysLys: 0.661 ± 0.031	2.447LysLeu: 2.447 ± 0.059	0.329LysMet: 0.329 ± 0.02	0.48LysAsn: 0.48 ± 0.025	1.546LysPro: 1.546 ± 0.055	0.979LysGln: 0.979 ± 0.033	1.664LysArg: 1.664 ± 0.046	1.034LysSer: 1.034 ± 0.035	1.155LysThr: 1.155 ± 0.036	1.64LysVal: 1.64 ± 0.046	0.153LysTrp: 0.153 ± 0.013	0.349LysTyr: 0.349 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	16.216LeuAla: 16.216 ± 0.171	1.326LeuCys: 1.326 ± 0.039	6.243LeuAsp: 6.243 ± 0.086	9.573LeuGlu: 9.573 ± 0.126	3.469LeuPhe: 3.469 ± 0.076	10.736LeuGly: 10.736 ± 0.134	2.526LeuHis: 2.526 ± 0.058	5.465LeuIle: 5.465 ± 0.093	2.884LeuLys: 2.884 ± 0.059	17.75LeuLeu: 17.75 ± 0.245	2.455LeuMet: 2.455 ± 0.054	2.877LeuAsn: 2.877 ± 0.06	7.999LeuPro: 7.999 ± 0.132	5.861LeuGln: 5.861 ± 0.083	9.964LeuArg: 9.964 ± 0.121	7.99LeuSer: 7.99 ± 0.091	5.299LeuThr: 5.299 ± 0.078	9.8LeuVal: 9.8 ± 0.147	1.918LeuTrp: 1.918 ± 0.057	1.958LeuTyr: 1.958 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.167MetAla: 3.167 ± 0.058	0.088MetCys: 0.088 ± 0.01	0.767MetAsp: 0.767 ± 0.029	1.162MetGlu: 1.162 ± 0.044	0.379MetPhe: 0.379 ± 0.019	1.742MetGly: 1.742 ± 0.049	0.321MetHis: 0.321 ± 0.021	0.756MetIle: 0.756 ± 0.03	0.516MetLys: 0.516 ± 0.025	2.05MetLeu: 2.05 ± 0.048	0.282MetMet: 0.282 ± 0.02	0.464MetAsn: 0.464 ± 0.021	1.311MetPro: 1.311 ± 0.037	0.656MetGln: 0.656 ± 0.026	1.174MetArg: 1.174 ± 0.036	1.114MetSer: 1.114 ± 0.034	1.116MetThr: 1.116 ± 0.04	1.673MetVal: 1.673 ± 0.042	0.103MetTrp: 0.103 ± 0.01	0.204MetTyr: 0.204 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.188AsnAla: 2.188 ± 0.051	0.259AsnCys: 0.259 ± 0.018	1.128AsnAsp: 1.128 ± 0.036	0.937AsnGlu: 0.937 ± 0.033	0.69AsnPhe: 0.69 ± 0.03	2.03AsnGly: 2.03 ± 0.052	0.464AsnHis: 0.464 ± 0.024	0.722AsnIle: 0.722 ± 0.03	0.498AsnLys: 0.498 ± 0.025	2.799AsnLeu: 2.799 ± 0.06	0.306AsnMet: 0.306 ± 0.022	0.573AsnAsn: 0.573 ± 0.028	1.789AsnPro: 1.789 ± 0.043	0.914AsnGln: 0.914 ± 0.032	1.837AsnArg: 1.837 ± 0.051	1.182AsnSer: 1.182 ± 0.032	1.031AsnThr: 1.031 ± 0.039	1.296AsnVal: 1.296 ± 0.04	0.368AsnTrp: 0.368 ± 0.022	0.478AsnTyr: 0.478 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	7.597ProAla: 7.597 ± 0.115	0.65ProCys: 0.65 ± 0.028	3.267ProAsp: 3.267 ± 0.061	4.597ProGlu: 4.597 ± 0.072	1.954ProPhe: 1.954 ± 0.046	5.636ProGly: 5.636 ± 0.092	1.143ProHis: 1.143 ± 0.03	2.25ProIle: 2.25 ± 0.049	1.083ProLys: 1.083 ± 0.04	8.117ProLeu: 8.117 ± 0.134	1.352ProMet: 1.352 ± 0.038	1.068ProAsn: 1.068 ± 0.036	3.829ProPro: 3.829 ± 0.086	2.524ProGln: 2.524 ± 0.06	3.723ProArg: 3.723 ± 0.074	4.053ProSer: 4.053 ± 0.07	2.584ProThr: 2.584 ± 0.063	4.385ProVal: 4.385 ± 0.077	1.235ProTrp: 1.235 ± 0.042	1.057ProTyr: 1.057 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	5.63GlnAla: 5.63 ± 0.089	0.303GlnCys: 0.303 ± 0.017	1.603GlnAsp: 1.603 ± 0.04	2.543GlnGlu: 2.543 ± 0.063	1.056GlnPhe: 1.056 ± 0.041	3.676GlnGly: 3.676 ± 0.072	0.844GlnHis: 0.844 ± 0.035	1.61GlnIle: 1.61 ± 0.043	0.935GlnLys: 0.935 ± 0.032	6.621GlnLeu: 6.621 ± 0.096	0.672GlnMet: 0.672 ± 0.03	0.705GlnAsn: 0.705 ± 0.034	3.075GlnPro: 3.075 ± 0.057	2.761GlnGln: 2.761 ± 0.078	4.39GlnArg: 4.39 ± 0.076	1.954GlnSer: 1.954 ± 0.052	1.451GlnThr: 1.451 ± 0.037	3.318GlnVal: 3.318 ± 0.065	0.711GlnTrp: 0.711 ± 0.031	0.47GlnTyr: 0.47 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	6.639ArgAla: 6.639 ± 0.093	1.108ArgCys: 1.108 ± 0.043	3.277ArgAsp: 3.277 ± 0.061	4.056ArgGlu: 4.056 ± 0.067	2.97ArgPhe: 2.97 ± 0.06	5.263ArgGly: 5.263 ± 0.076	2.01ArgHis: 2.01 ± 0.054	3.191ArgIle: 3.191 ± 0.051	1.426ArgLys: 1.426 ± 0.044	11.374ArgLeu: 11.374 ± 0.157	1.563ArgMet: 1.563 ± 0.046	1.557ArgAsn: 1.557 ± 0.042	4.467ArgPro: 4.467 ± 0.082	4.674ArgGln: 4.674 ± 0.076	7.529ArgArg: 7.529 ± 0.125	5.004ArgSer: 5.004 ± 0.081	2.953ArgThr: 2.953 ± 0.061	4.62ArgVal: 4.62 ± 0.078	1.935ArgTrp: 1.935 ± 0.056	1.681ArgTyr: 1.681 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	6.406SerAla: 6.406 ± 0.093	0.756SerCys: 0.756 ± 0.028	2.964SerAsp: 2.964 ± 0.054	3.206SerGlu: 3.206 ± 0.06	2.127SerPhe: 2.127 ± 0.048	6.367SerGly: 6.367 ± 0.086	1.301SerHis: 1.301 ± 0.04	2.2SerIle: 2.2 ± 0.048	1.223SerLys: 1.223 ± 0.041	7.818SerLeu: 7.818 ± 0.092	1.217SerMet: 1.217 ± 0.035	1.323SerAsn: 1.323 ± 0.038	3.809SerPro: 3.809 ± 0.07	2.43SerGln: 2.43 ± 0.059	4.696SerArg: 4.696 ± 0.084	4.305SerSer: 4.305 ± 0.087	3.079SerThr: 3.079 ± 0.064	3.64SerVal: 3.64 ± 0.062	1.219SerTrp: 1.219 ± 0.036	1.161SerTyr: 1.161 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	5.446ThrAla: 5.446 ± 0.079	0.415ThrCys: 0.415 ± 0.022	2.073ThrAsp: 2.073 ± 0.058	1.935ThrGlu: 1.935 ± 0.049	1.348ThrPhe: 1.348 ± 0.042	4.1ThrGly: 4.1 ± 0.083	0.804ThrHis: 0.804 ± 0.031	2.043ThrIle: 2.043 ± 0.053	0.902ThrLys: 0.902 ± 0.03	5.542ThrLeu: 5.542 ± 0.081	0.783ThrMet: 0.783 ± 0.03	0.986ThrAsn: 0.986 ± 0.037	3.505ThrPro: 3.505 ± 0.071	1.342ThrGln: 1.342 ± 0.039	2.531ThrArg: 2.531 ± 0.053	2.826ThrSer: 2.826 ± 0.065	2.577ThrThr: 2.577 ± 0.054	3.446ThrVal: 3.446 ± 0.065	0.662ThrTrp: 0.662 ± 0.028	0.995ThrTyr: 0.995 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	8.1ValAla: 8.1 ± 0.105	0.691ValCys: 0.691 ± 0.026	3.405ValAsp: 3.405 ± 0.068	4.389ValGlu: 4.389 ± 0.076	2.27ValPhe: 2.27 ± 0.051	5.49ValGly: 5.49 ± 0.084	1.206ValHis: 1.206 ± 0.039	3.411ValIle: 3.411 ± 0.068	1.565ValLys: 1.565 ± 0.048	9.384ValLeu: 9.384 ± 0.11	1.612ValMet: 1.612 ± 0.04	1.745ValAsn: 1.745 ± 0.043	3.817ValPro: 3.817 ± 0.073	2.488ValGln: 2.488 ± 0.052	4.531ValArg: 4.531 ± 0.074	4.121ValSer: 4.121 ± 0.066	3.013ValThr: 3.013 ± 0.06	6.282ValVal: 6.282 ± 0.099	0.884ValTrp: 0.884 ± 0.03	1.079ValTyr: 1.079 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.401TrpAla: 1.401 ± 0.04	0.249TrpCys: 0.249 ± 0.019	0.696TrpAsp: 0.696 ± 0.029	0.755TrpGlu: 0.755 ± 0.031	0.617TrpPhe: 0.617 ± 0.028	1.314TrpGly: 1.314 ± 0.044	0.396TrpHis: 0.396 ± 0.023	0.819TrpIle: 0.819 ± 0.032	0.373TrpLys: 0.373 ± 0.019	2.844TrpLeu: 2.844 ± 0.061	0.42TrpMet: 0.42 ± 0.022	0.383TrpAsn: 0.383 ± 0.019	1.057TrpPro: 1.057 ± 0.039	0.987TrpGln: 0.987 ± 0.038	1.691TrpArg: 1.691 ± 0.044	1.218TrpSer: 1.218 ± 0.035	0.602TrpThr: 0.602 ± 0.03	1.019TrpVal: 1.019 ± 0.034	0.44TrpTrp: 0.44 ± 0.024	0.287TrpTyr: 0.287 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.777TyrAla: 1.777 ± 0.046	0.216TyrCys: 0.216 ± 0.015	1.005TyrAsp: 1.005 ± 0.033	0.884TyrGlu: 0.884 ± 0.029	0.583TyrPhe: 0.583 ± 0.024	1.973TyrGly: 1.973 ± 0.053	0.379TyrHis: 0.379 ± 0.022	0.608TyrIle: 0.608 ± 0.028	0.379TyrLys: 0.379 ± 0.019	2.179TyrLeu: 2.179 ± 0.053	0.268TyrMet: 0.268 ± 0.016	0.44TyrAsn: 0.44 ± 0.026	1.013TyrPro: 1.013 ± 0.038	0.679TyrGln: 0.679 ± 0.029	1.683TyrArg: 1.683 ± 0.04	1.059TyrSer: 1.059 ± 0.039	0.771TyrThr: 0.771 ± 0.029	1.134TyrVal: 1.134 ± 0.038	0.351TyrTrp: 0.351 ± 0.019	0.41TyrTyr: 0.41 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3316 proteins (883610 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---