Setosphaeria turcica (strain 28A) (Northern leaf blight fungus) (Exserohilum turcicum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Exserohilum; Exserohilum turcicum

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R0IUY1|R0IUY1_SETT2 Uncharacterized protein OS=Setosphaeria turcica (strain 28A) OX=671987 GN=SETTUDRAFT_27257 PE=4 SV=1
MM1 pKa = 7.45AWSSKK6 pKa = 8.22QASKK10 pKa = 11.01PCMQSSLSGNWGLGNVDD27 pKa = 3.91GKK29 pKa = 10.48QASTCAWALFGGGNFFGGLQDD50 pKa = 3.78AGEE53 pKa = 5.29CISQAASHH61 pKa = 7.46DD62 pKa = 4.27DD63 pKa = 3.89DD64 pKa = 7.59DD65 pKa = 7.06DD66 pKa = 7.34DD67 pKa = 4.32EE68 pKa = 4.98GQWGRR73 pKa = 11.84SVQFGKK79 pKa = 10.69AIGCMHH85 pKa = 7.28ACICSLPAVLSGVGSSSPDD104 pKa = 4.07DD105 pKa = 5.74DD106 pKa = 6.94DD107 pKa = 7.52DD108 pKa = 7.47DD109 pKa = 6.93DD110 pKa = 6.57DD111 pKa = 5.13EE112 pKa = 6.04EE113 pKa = 4.33EE114 pKa = 4.22ASS116 pKa = 3.6

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R0IYI7|R0IYI7_SETT2 Uncharacterized protein OS=Setosphaeria turcica (strain 28A) OX=671987 GN=SETTUDRAFT_147049 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11687

0

11687

5320234

49

7200

455.2

50.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.166 ± 0.018

1.264 ± 0.008

5.673 ± 0.015

6.038 ± 0.022

3.605 ± 0.013

6.714 ± 0.024

2.558 ± 0.01

4.714 ± 0.015

5.019 ± 0.019

8.618 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.009

3.663 ± 0.013

6.179 ± 0.029

4.19 ± 0.016

6.055 ± 0.021

7.976 ± 0.025

5.993 ± 0.013

6.031 ± 0.017

1.457 ± 0.008

2.801 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski