Thiohalocapsa sp. PB-PSB1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Thiohalocapsa; unclassified Thiohalocapsa

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4JJW4|V4JJW4_9GAMM Uncharacterized protein (Fragment) OS=Thiohalocapsa sp. PB-PSB1 OX=1385625 GN=N838_30920 PE=4 SV=1
MM1 pKa = 5.93THH3 pKa = 7.01KK4 pKa = 9.97RR5 pKa = 11.84TAWWNRR11 pKa = 11.84LALSAIVGLLAAIGTLTAAPSRR33 pKa = 11.84AEE35 pKa = 4.14TIAYY39 pKa = 9.61GYY41 pKa = 10.97DD42 pKa = 3.03PGNRR46 pKa = 11.84LEE48 pKa = 4.16SVEE51 pKa = 4.15YY52 pKa = 10.7DD53 pKa = 3.2DD54 pKa = 4.96GQSIRR59 pKa = 11.84YY60 pKa = 9.58VYY62 pKa = 11.01DD63 pKa = 3.12NLGNQLVRR71 pKa = 11.84LTLAAPEE78 pKa = 4.39ANNPPGTVTPGVADD92 pKa = 3.91GTEE95 pKa = 3.9LTYY98 pKa = 10.29TAVTLDD104 pKa = 3.19WSAAVDD110 pKa = 4.01PDD112 pKa = 3.64AGDD115 pKa = 3.21AVVYY119 pKa = 9.5YY120 pKa = 9.77LHH122 pKa = 7.27LGTEE126 pKa = 4.36ADD128 pKa = 3.78PPLVYY133 pKa = 10.68SGWEE137 pKa = 3.56RR138 pKa = 11.84SYY140 pKa = 10.94TPPYY144 pKa = 10.25SLQPLTTYY152 pKa = 9.75FWSVVARR159 pKa = 11.84DD160 pKa = 3.77SQGAEE165 pKa = 4.1SVSGPWTFITGNEE178 pKa = 4.25PPTPIIDD185 pKa = 4.08ASPTEE190 pKa = 4.66AIVPYY195 pKa = 9.17TANLTDD201 pKa = 4.3ASTSTDD207 pKa = 3.24DD208 pKa = 5.62AIASRR213 pKa = 11.84AWDD216 pKa = 4.07FLCDD220 pKa = 3.85GSIDD224 pKa = 3.81ATTSTASYY232 pKa = 9.19YY233 pKa = 10.89VSAAGDD239 pKa = 3.8YY240 pKa = 7.74EE241 pKa = 3.98ICLAVTDD248 pKa = 4.12EE249 pKa = 4.55AGATEE254 pKa = 4.38STSVTLYY261 pKa = 11.09GRR263 pKa = 11.84IDD265 pKa = 3.53TDD267 pKa = 3.4GDD269 pKa = 4.21GVFDD273 pKa = 5.04IADD276 pKa = 3.9NCPADD281 pKa = 4.37ANADD285 pKa = 3.77QYY287 pKa = 12.23NLDD290 pKa = 4.16GDD292 pKa = 4.45DD293 pKa = 5.34LGDD296 pKa = 3.59VCDD299 pKa = 5.55DD300 pKa = 4.36DD301 pKa = 6.37RR302 pKa = 11.84DD303 pKa = 4.01GDD305 pKa = 4.31GVTNAADD312 pKa = 4.29PFPDD316 pKa = 3.82DD317 pKa = 3.81ARR319 pKa = 11.84YY320 pKa = 7.47TADD323 pKa = 3.73SDD325 pKa = 3.88GDD327 pKa = 4.22GIGDD331 pKa = 3.24SWEE334 pKa = 3.75IAEE337 pKa = 4.8FGDD340 pKa = 6.22LITADD345 pKa = 4.12DD346 pKa = 4.0TTDD349 pKa = 3.62FDD351 pKa = 5.44GDD353 pKa = 3.72GSSDD357 pKa = 3.61LDD359 pKa = 3.53EE360 pKa = 4.87FRR362 pKa = 11.84YY363 pKa = 10.78DD364 pKa = 4.21SDD366 pKa = 3.63PQYY369 pKa = 11.55APPFVDD375 pKa = 3.76TAPIAAGGDD384 pKa = 3.19HH385 pKa = 6.92SLAQRR390 pKa = 11.84TDD392 pKa = 2.59GRR394 pKa = 11.84LYY396 pKa = 10.04AWGYY400 pKa = 9.58NGNGQLGDD408 pKa = 3.47GSYY411 pKa = 11.06TNRR414 pKa = 11.84NYY416 pKa = 10.41PAFVRR421 pKa = 11.84DD422 pKa = 3.47DD423 pKa = 3.49TGAFVYY429 pKa = 10.22DD430 pKa = 3.74IQDD433 pKa = 3.01IAAGGDD439 pKa = 3.14HH440 pKa = 6.61SLAVLGDD447 pKa = 3.61GSLISWGYY455 pKa = 9.77NGYY458 pKa = 9.23GQLGDD463 pKa = 3.62GTNTRR468 pKa = 11.84RR469 pKa = 11.84ADD471 pKa = 3.59PVAVIDD477 pKa = 4.75GYY479 pKa = 11.46GDD481 pKa = 3.67PVIDD485 pKa = 4.13AVSVAAGANHH495 pKa = 6.58SLALLDD501 pKa = 5.54DD502 pKa = 4.75GSLLAWGYY510 pKa = 10.91NGYY513 pKa = 9.24GQLGDD518 pKa = 3.55GTTTSKK524 pKa = 9.36STPVRR529 pKa = 11.84VRR531 pKa = 11.84DD532 pKa = 3.75AVGDD536 pKa = 3.83TLTGIIAVAAGQNFSLALRR555 pKa = 11.84SDD557 pKa = 3.53GTVWAWGRR565 pKa = 11.84NYY567 pKa = 10.07QGQLGDD573 pKa = 3.85GTNSNSASAQRR584 pKa = 11.84VLDD587 pKa = 3.79ARR589 pKa = 11.84GMPINGVVAVAAGFRR604 pKa = 11.84HH605 pKa = 5.91GLALSEE611 pKa = 4.84AGTAVAWGDD620 pKa = 3.21NDD622 pKa = 3.67YY623 pKa = 11.45GQLGDD628 pKa = 4.05GFTVDD633 pKa = 3.04RR634 pKa = 11.84RR635 pKa = 11.84VPVQVIDD642 pKa = 4.02AFGTPVDD649 pKa = 4.11GMSALAAGEE658 pKa = 4.09WHH660 pKa = 6.52SLALASGGDD669 pKa = 3.51VLAWGRR675 pKa = 11.84NNLGQLGEE683 pKa = 4.43GTLSDD688 pKa = 3.7SSIPTSLGLTGITGIDD704 pKa = 3.28AGSEE708 pKa = 3.77HH709 pKa = 6.75SLAVTDD715 pKa = 5.35DD716 pKa = 3.54LTAFAWGRR724 pKa = 11.84NALFQLGDD732 pKa = 3.35GTATDD737 pKa = 3.34RR738 pKa = 11.84RR739 pKa = 11.84SPVVVIDD746 pKa = 4.06NNLAPIFPVGKK757 pKa = 9.65PSTDD761 pKa = 3.32PAADD765 pKa = 3.41SDD767 pKa = 4.47GDD769 pKa = 4.19GEE771 pKa = 4.63PDD773 pKa = 3.34STDD776 pKa = 2.99AFPYY780 pKa = 10.48DD781 pKa = 3.09PAYY784 pKa = 10.87RR785 pKa = 11.84LDD787 pKa = 3.85ADD789 pKa = 3.93GDD791 pKa = 4.34GIADD795 pKa = 3.54EE796 pKa = 4.54WEE798 pKa = 3.97LANFGDD804 pKa = 5.35LLTAGIGTDD813 pKa = 3.58YY814 pKa = 11.47DD815 pKa = 4.17GDD817 pKa = 3.92GLLDD821 pKa = 3.65SEE823 pKa = 4.55EE824 pKa = 4.53FEE826 pKa = 4.8HH827 pKa = 8.0GSDD830 pKa = 3.09ATVAPEE836 pKa = 3.79MVEE839 pKa = 4.63DD840 pKa = 4.46VVATGANHH848 pKa = 6.1TLALRR853 pKa = 11.84TDD855 pKa = 3.45GRR857 pKa = 11.84VLAWGYY863 pKa = 10.87NGYY866 pKa = 9.22GQLGNGTTQNQSSVSYY882 pKa = 8.34THH884 pKa = 6.94

Molecular weight: 92.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4JDW3|V4JDW3_9GAMM Oxidoreductase OS=Thiohalocapsa sp. PB-PSB1 OX=1385625 GN=N838_02070 PE=4 SV=1
KKK2 pKa = 9.53WRR4 pKa = 11.84RR5 pKa = 11.84ALYYY9 pKa = 10.1QLRR12 pKa = 11.84RR13 pKa = 11.84HHH15 pKa = 6.08GGVFRR20 pKa = 11.84EEE22 pKa = 4.33ARR24 pKa = 11.84HHH26 pKa = 5.39KK27 pKa = 10.49DD28 pKa = 3.2RR29 pKa = 11.84IEEE32 pKa = 3.94EE33 pKa = 4.17HHH35 pKa = 5.89MPDDD39 pKa = 3.4HH40 pKa = 5.82HHH42 pKa = 5.8PISIPPKKK50 pKa = 8.98DD51 pKa = 3.59VSQVVGYYY59 pKa = 10.2RR60 pKa = 11.84GKKK63 pKa = 10.03AIHHH67 pKa = 6.48ARR69 pKa = 11.84VYYY72 pKa = 10.38EEE74 pKa = 4.06KKK76 pKa = 9.25NFAGQHHH83 pKa = 4.7RR84 pKa = 11.84ARR86 pKa = 11.84GCFVSTVGRR95 pKa = 11.84DDD97 pKa = 2.94EE98 pKa = 4.21TRR100 pKa = 11.84SEEE103 pKa = 4.42TSSTSNVKKK112 pKa = 10.19KKK114 pKa = 10.92DD115 pKa = 3.86IN

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6200

0

6200

1699814

29

3305

274.2

30.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.996 ± 0.041

1.105 ± 0.014

6.178 ± 0.029

5.759 ± 0.033

3.583 ± 0.02

7.706 ± 0.031

2.323 ± 0.016

5.139 ± 0.024

2.863 ± 0.024

11.208 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.016

2.888 ± 0.022

5.213 ± 0.026

4.109 ± 0.024

7.521 ± 0.04

5.654 ± 0.028

4.935 ± 0.025

6.576 ± 0.03

1.491 ± 0.016

2.483 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski