Tropicibacter naphthalenivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Tropicibacter

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7M0G0|A0A0N7M0G0_9RHOB Uncharacterized protein OS=Tropicibacter naphthalenivorans OX=441103 GN=TRN7648_02949 PE=4 SV=1
MM1 pKa = 7.34PAIYY5 pKa = 8.7TWQHH9 pKa = 4.91VPTHH13 pKa = 6.64KK14 pKa = 10.54YY15 pKa = 9.36VPAYY19 pKa = 9.36DD20 pKa = 2.83QWYY23 pKa = 8.43YY24 pKa = 11.63DD25 pKa = 4.41KK26 pKa = 11.18IHH28 pKa = 6.15ITGPGAIAYY37 pKa = 8.78GGYY40 pKa = 10.58GDD42 pKa = 5.19DD43 pKa = 3.33VYY45 pKa = 11.69YY46 pKa = 10.6HH47 pKa = 6.12YY48 pKa = 11.05GVRR51 pKa = 11.84SYY53 pKa = 11.41SSPRR57 pKa = 11.84DD58 pKa = 3.42KK59 pKa = 11.24VIVEE63 pKa = 4.59FADD66 pKa = 3.07QGYY69 pKa = 10.03DD70 pKa = 3.08IVHH73 pKa = 6.6VDD75 pKa = 3.71PLADD79 pKa = 3.59IVAQLYY85 pKa = 9.17FVEE88 pKa = 5.66EE89 pKa = 3.86IHH91 pKa = 6.68LGCSSLSRR99 pKa = 11.84EE100 pKa = 5.27IYY102 pKa = 8.93GTWQAQAFYY111 pKa = 10.31GLHH114 pKa = 6.59RR115 pKa = 11.84GDD117 pKa = 3.25TLYY120 pKa = 10.94AGEE123 pKa = 5.15GDD125 pKa = 3.52DD126 pKa = 3.79TLYY129 pKa = 10.96TRR131 pKa = 11.84VDD133 pKa = 2.79VDD135 pKa = 3.65LRR137 pKa = 11.84KK138 pKa = 10.2EE139 pKa = 3.9NAEE142 pKa = 4.02FEE144 pKa = 4.73HH145 pKa = 7.72VIATYY150 pKa = 10.58DD151 pKa = 3.75GNSEE155 pKa = 3.87NSNLNFQNLGWRR167 pKa = 11.84FVRR170 pKa = 11.84GEE172 pKa = 3.84PWTVHH177 pKa = 4.34GSEE180 pKa = 4.47RR181 pKa = 11.84GDD183 pKa = 4.14DD184 pKa = 3.52IVGGGSLDD192 pKa = 3.22KK193 pKa = 10.99LYY195 pKa = 10.86GYY197 pKa = 10.7GGDD200 pKa = 4.21DD201 pKa = 3.6SLVGAGLNDD210 pKa = 3.7YY211 pKa = 11.16LSGGEE216 pKa = 4.67GNDD219 pKa = 3.42SLYY222 pKa = 11.38GGVDD226 pKa = 3.11NDD228 pKa = 4.19TLYY231 pKa = 11.64GDD233 pKa = 4.83AGDD236 pKa = 5.5DD237 pKa = 3.82YY238 pKa = 11.34LEE240 pKa = 4.69SGAGHH245 pKa = 6.97DD246 pKa = 3.87EE247 pKa = 4.63VYY249 pKa = 10.9GGTGDD254 pKa = 3.65DD255 pKa = 3.9TLIGSDD261 pKa = 4.8GNDD264 pKa = 3.16TLAGGDD270 pKa = 3.46GDD272 pKa = 4.81DD273 pKa = 4.57FYY275 pKa = 11.86RR276 pKa = 11.84DD277 pKa = 3.27VSLGSVIIEE286 pKa = 4.38DD287 pKa = 4.02ALGGDD292 pKa = 4.0DD293 pKa = 4.13TQIITQGATRR303 pKa = 11.84QGEE306 pKa = 4.23NVEE309 pKa = 3.82RR310 pKa = 11.84LIYY313 pKa = 9.65EE314 pKa = 4.18RR315 pKa = 11.84SYY317 pKa = 10.93VLGVEE322 pKa = 4.47VRR324 pKa = 11.84QDD326 pKa = 3.42LYY328 pKa = 11.88VEE330 pKa = 4.67GNSVGNSLKK339 pKa = 10.2TGIGDD344 pKa = 3.42DD345 pKa = 4.1TLRR348 pKa = 11.84GMAGDD353 pKa = 3.97DD354 pKa = 3.42TLMGGSGADD363 pKa = 3.7LYY365 pKa = 11.21EE366 pKa = 5.17GGAGRR371 pKa = 11.84DD372 pKa = 4.04FIEE375 pKa = 4.82DD376 pKa = 3.12RR377 pKa = 11.84GTRR380 pKa = 11.84TVTDD384 pKa = 4.43ADD386 pKa = 3.08TGMQVASGLDD396 pKa = 3.41DD397 pKa = 3.81TLDD400 pKa = 3.93GGLGADD406 pKa = 4.65FMRR409 pKa = 11.84GGLGSDD415 pKa = 4.4LYY417 pKa = 10.77IVDD420 pKa = 3.68NAGDD424 pKa = 3.65VALDD428 pKa = 3.57SAEE431 pKa = 4.15DD432 pKa = 3.42AGAEE436 pKa = 4.02DD437 pKa = 4.71TVWFHH442 pKa = 7.28ASEE445 pKa = 4.89GDD447 pKa = 3.34LTYY450 pKa = 10.9NASVMNMRR458 pKa = 11.84EE459 pKa = 4.09VEE461 pKa = 4.17TLRR464 pKa = 11.84IDD466 pKa = 3.89LSAAGQVSLSTDD478 pKa = 2.55ARR480 pKa = 11.84ADD482 pKa = 3.87HH483 pKa = 7.04IIGNAASDD491 pKa = 4.02TIDD494 pKa = 3.38GGFGNDD500 pKa = 3.38LNLGGRR506 pKa = 11.84GTLGRR511 pKa = 11.84DD512 pKa = 3.53GQSLSDD518 pKa = 3.6VLGEE522 pKa = 4.19EE523 pKa = 4.5VAGDD527 pKa = 3.66DD528 pKa = 5.05ARR530 pKa = 11.84THH532 pKa = 6.92DD533 pKa = 4.17VLKK536 pKa = 11.02GGAGDD541 pKa = 3.74DD542 pKa = 4.07TIFASVGDD550 pKa = 4.38SVDD553 pKa = 3.26GGAGQDD559 pKa = 3.42EE560 pKa = 5.29LFVNAAVGPDD570 pKa = 3.16QITNVEE576 pKa = 3.9RR577 pKa = 11.84TVFLEE582 pKa = 4.0ADD584 pKa = 3.5TEE586 pKa = 4.5LFVGGTGTTVITQNDD601 pKa = 3.91SFVQGNAFALTGQGVQLLAQGGDD624 pKa = 3.59DD625 pKa = 3.69SVTGTGSVFVDD636 pKa = 4.08LGTGNDD642 pKa = 3.67SDD644 pKa = 3.95THH646 pKa = 7.47LLGTGGTTRR655 pKa = 11.84TVIVGGEE662 pKa = 4.16GGDD665 pKa = 3.8TVTLPVDD672 pKa = 4.38YY673 pKa = 10.13EE674 pKa = 4.44VLWITEE680 pKa = 3.96ATATEE685 pKa = 4.68TVTRR689 pKa = 11.84WDD691 pKa = 4.0ADD693 pKa = 3.69SQSWIVAEE701 pKa = 3.9YY702 pKa = 10.46DD703 pKa = 3.74LPVLQMVGQAEE714 pKa = 4.36VAPGQIVDD722 pKa = 3.54YY723 pKa = 9.1TFAVHH728 pKa = 6.21GVEE731 pKa = 5.08HH732 pKa = 7.18IEE734 pKa = 4.0LNYY737 pKa = 10.62QGVQVSATPVEE748 pKa = 4.45LLPVAPEE755 pKa = 3.94EE756 pKa = 3.96LTPDD760 pKa = 3.48SFTATHH766 pKa = 5.91ATIEE770 pKa = 4.13AALLGDD776 pKa = 4.35PAALGYY782 pKa = 8.4GTGAQLVRR790 pKa = 11.84YY791 pKa = 8.41VISLDD796 pKa = 3.44TSVGVPVFSVMTTDD810 pKa = 3.34NNVPFDD816 pKa = 4.27GEE818 pKa = 3.81PMYY821 pKa = 10.88RR822 pKa = 11.84FVDD825 pKa = 4.04APDD828 pKa = 3.83GTPALAGLTNLSAAEE843 pKa = 3.91ATALFSFVTDD853 pKa = 3.38GDD855 pKa = 3.93GTTRR859 pKa = 11.84PGTLTLNSLPEE870 pKa = 4.61AGSDD874 pKa = 4.68LIQDD878 pKa = 4.93LSTTDD883 pKa = 3.56NPEE886 pKa = 4.26DD887 pKa = 3.87PPASVGVIWVEE898 pKa = 3.97AYY900 pKa = 10.32DD901 pKa = 3.31ITGRR905 pKa = 11.84STGAYY910 pKa = 7.72PLEE913 pKa = 4.42VNFAQIPGQWIAHH926 pKa = 5.64TSDD929 pKa = 4.66DD930 pKa = 4.07RR931 pKa = 11.84VTDD934 pKa = 4.36LSQSPASRR942 pKa = 11.84DD943 pKa = 3.75TIIGGVWNDD952 pKa = 4.13TILSGDD958 pKa = 4.04GGDD961 pKa = 3.91EE962 pKa = 4.13IYY964 pKa = 11.07GGGSDD969 pKa = 4.4HH970 pKa = 7.4SGTEE974 pKa = 3.94QIDD977 pKa = 3.72AGWGNDD983 pKa = 3.28FVQVISNGLTYY994 pKa = 10.65HH995 pKa = 6.76FDD997 pKa = 3.14INYY1000 pKa = 9.96EE1001 pKa = 3.98IVPWDD1006 pKa = 3.9AGSTPTT1012 pKa = 5.02

Molecular weight: 106.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1GTW0|A0A0P1GTW0_9RHOB Hydroxyacylglutathione hydrolase OS=Tropicibacter naphthalenivorans OX=441103 GN=TRN7648_01509 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4206

0

4206

1340933

29

9577

318.8

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.321 ± 0.057

0.884 ± 0.012

6.478 ± 0.07

5.779 ± 0.037

3.659 ± 0.023

8.883 ± 0.066

2.028 ± 0.022

4.977 ± 0.025

3.197 ± 0.034

9.951 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.024

2.627 ± 0.021

5.038 ± 0.036

3.455 ± 0.024

6.366 ± 0.051

4.984 ± 0.025

5.757 ± 0.042

7.204 ± 0.029

1.389 ± 0.016

2.233 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski