Flavobacterium sp. TAB 87
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3323 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A105TSB4|A0A105TSB4_9FLAO Catalase OS=Flavobacterium sp. TAB 87 OX=1729581 GN=katE_1 PE=3 SV=1
MM1 pKa = 6.91 YY2 pKa = 10.36 FSVKK6 pKa = 10.3 KK7 pKa = 10.83 LFLHH11 pKa = 6.5 KK12 pKa = 10.52 LNRR15 pKa = 11.84 LNMYY19 pKa = 7.13 WTLEE23 pKa = 4.01 LASYY27 pKa = 10.74 LSDD30 pKa = 4.59 APWPANKK37 pKa = 10.23 DD38 pKa = 3.49 EE39 pKa = 5.48 LIDD42 pKa = 3.63 YY43 pKa = 8.89 AIRR46 pKa = 11.84 AGAPLEE52 pKa = 4.15 VVEE55 pKa = 4.43 NLQSIEE61 pKa = 4.26 DD62 pKa = 3.48 EE63 pKa = 4.53 GEE65 pKa = 3.82 IYY67 pKa = 10.83 EE68 pKa = 4.34 SMEE71 pKa = 4.81 EE72 pKa = 3.72 IWPDD76 pKa = 3.41 YY77 pKa = 8.92 PTDD80 pKa = 4.86 EE81 pKa = 5.08 DD82 pKa = 4.52 YY83 pKa = 11.55 LWNEE87 pKa = 4.3 DD88 pKa = 3.47 EE89 pKa = 4.46 YY90 pKa = 11.97
Molecular weight: 10.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.973
IPC_protein 3.884
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.745
EMBOSS 3.757
Sillero 3.999
Patrickios 0.896
IPC_peptide 3.834
IPC2_peptide 3.986
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|A0A0X7BCZ7|A0A0X7BCZ7_9FLAO Uncharacterized protein OS=Flavobacterium sp. TAB 87 OX=1729581 GN=AP058_01236 PE=4 SV=1
MM1 pKa = 6.93 VKK3 pKa = 10.11 FWIISIVIYY12 pKa = 10.42 IISTYY17 pKa = 10.62 LLSKK21 pKa = 9.6 FTLKK25 pKa = 10.73 ANRR28 pKa = 11.84 RR29 pKa = 11.84 EE30 pKa = 3.88 VGEE33 pKa = 4.29 RR34 pKa = 11.84 IWRR37 pKa = 11.84 FGSGRR42 pKa = 11.84 STYY45 pKa = 9.31 WRR47 pKa = 11.84 ILTLCSFGITVVVMLVLHH65 pKa = 7.02 WIGIVITT72 pKa = 4.16
Molecular weight: 8.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 10.028
IPC_protein 10.847
Toseland 10.716
ProMoST 10.496
Dawson 10.847
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.038
Grimsley 10.921
Solomon 10.965
Lehninger 10.921
Nozaki 10.701
DTASelect 10.599
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.774
Patrickios 10.833
IPC_peptide 10.965
IPC2_peptide 9.721
IPC2.peptide.svr19 8.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3323
0
3323
1112781
29
5371
334.9
37.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.791 ± 0.051
0.765 ± 0.014
5.315 ± 0.027
6.391 ± 0.045
5.293 ± 0.034
6.111 ± 0.046
1.685 ± 0.021
8.083 ± 0.039
7.96 ± 0.043
9.357 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.24 ± 0.021
6.125 ± 0.034
3.254 ± 0.022
3.608 ± 0.025
3.269 ± 0.027
6.597 ± 0.031
5.87 ± 0.051
6.278 ± 0.034
0.999 ± 0.016
4.01 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here