Bacillus caseinilyticus

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3GMA5|A0A1H3GMA5_9BACI Uncharacterized protein OS=Bacillus caseinilyticus OX=1503961 GN=SAMN05421736_101218 PE=4 SV=1
MM1 pKa = 7.84PIKK4 pKa = 10.26KK5 pKa = 9.81RR6 pKa = 11.84YY7 pKa = 9.4LFLASLFMTALLFGCSDD24 pKa = 3.42NSNNADD30 pKa = 4.36DD31 pKa = 4.57DD32 pKa = 4.25TGSQEE37 pKa = 4.01PVADD41 pKa = 4.36EE42 pKa = 4.85SSDD45 pKa = 3.98SADD48 pKa = 3.33SDD50 pKa = 3.7SGRR53 pKa = 11.84VLTDD57 pKa = 3.33AVGHH61 pKa = 5.98EE62 pKa = 4.48VTIPDD67 pKa = 3.9APEE70 pKa = 4.52KK71 pKa = 10.55IIASYY76 pKa = 11.13LEE78 pKa = 4.9DD79 pKa = 3.81YY80 pKa = 10.96LVALDD85 pKa = 3.99VTPAAQWSILDD96 pKa = 3.81GQGLQDD102 pKa = 3.76YY103 pKa = 10.07LQDD106 pKa = 3.72YY107 pKa = 8.65LQAVPPIPYY116 pKa = 8.51EE117 pKa = 3.82LPYY120 pKa = 10.91EE121 pKa = 4.42SVTSFSPDD129 pKa = 3.12LLLISSAEE137 pKa = 3.89AVDD140 pKa = 3.76DD141 pKa = 4.48GKK143 pKa = 11.29YY144 pKa = 9.51EE145 pKa = 4.1QYY147 pKa = 11.45SKK149 pKa = 10.72IAPTYY154 pKa = 10.76VVGADD159 pKa = 3.61NNSDD163 pKa = 2.81WRR165 pKa = 11.84EE166 pKa = 3.86NLLTIAEE173 pKa = 4.25VLDD176 pKa = 4.45LEE178 pKa = 5.25NKK180 pKa = 9.34AQQLLASYY188 pKa = 10.16DD189 pKa = 3.75EE190 pKa = 4.49KK191 pKa = 11.42AQAAKK196 pKa = 10.83EE197 pKa = 4.06EE198 pKa = 4.08IQAAIGTDD206 pKa = 3.2ATAAALWLVADD217 pKa = 3.9SFFIVSEE224 pKa = 4.23NVSSGAVLYY233 pKa = 9.84EE234 pKa = 4.11DD235 pKa = 5.33LGIAVPDD242 pKa = 3.69VVKK245 pKa = 10.67EE246 pKa = 4.0ISGDD250 pKa = 3.57AAANWSPISLEE261 pKa = 4.15EE262 pKa = 4.03IATLEE267 pKa = 4.12ADD269 pKa = 3.8HH270 pKa = 6.91LFLINSDD277 pKa = 3.71NASGAEE283 pKa = 3.95MLNDD287 pKa = 4.31PVWQSIPAVKK297 pKa = 10.27NGQVYY302 pKa = 9.94EE303 pKa = 4.19YY304 pKa = 10.89GPEE307 pKa = 4.16TSWLYY312 pKa = 9.9TGPIASAQMIDD323 pKa = 3.74HH324 pKa = 5.86VVEE327 pKa = 4.42SLVKK331 pKa = 10.51

Molecular weight: 35.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3TLQ3|A0A1H3TLQ3_9BACI Uncharacterized protein OS=Bacillus caseinilyticus OX=1503961 GN=SAMN05421736_11530 PE=4 SV=1
MM1 pKa = 7.2ITQTTVPDD9 pKa = 3.51VCFVRR14 pKa = 11.84PRR16 pKa = 11.84LTYY19 pKa = 10.48KK20 pKa = 10.76RR21 pKa = 11.84IAGCLLLAAVPIRR34 pKa = 11.84LVPLLLGSGTSFFPLKK50 pKa = 10.52QSS52 pKa = 3.18

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5197

0

5197

1567055

25

3823

301.5

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.698 ± 0.035

0.786 ± 0.012

5.112 ± 0.028

7.512 ± 0.042

4.576 ± 0.028

6.898 ± 0.029

2.198 ± 0.019

7.309 ± 0.032

6.122 ± 0.033

9.797 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.018

4.116 ± 0.025

3.697 ± 0.022

3.844 ± 0.022

4.479 ± 0.029

5.845 ± 0.022

5.414 ± 0.023

7.065 ± 0.027

1.136 ± 0.014

3.616 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski