Rhizoctonia oryzae-sativae mitovirus 1
Average proteome isoelectric point is 8.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166H5D4|A0A166H5D4_9VIRU Putative RNA-dependent RNA polymerase OS=Rhizoctonia oryzae-sativae mitovirus 1 OX=1837089 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.24 IINTTINNKK11 pKa = 8.87 TNTNFLSFSSGYY23 pKa = 9.77 SFSSFSPNATNSTTVKK39 pKa = 10.44 DD40 pKa = 3.89 LRR42 pKa = 11.84 QSIVPGINVVRR53 pKa = 11.84 NSTRR57 pKa = 11.84 LARR60 pKa = 11.84 SIALLTAYY68 pKa = 9.65 IEE70 pKa = 4.1 IQLRR74 pKa = 11.84 DD75 pKa = 3.73 LDD77 pKa = 3.94 DD78 pKa = 3.63 TSRR81 pKa = 11.84 MVLKK85 pKa = 10.75 QFMTEE90 pKa = 4.16 LEE92 pKa = 4.38 SMYY95 pKa = 9.51 HH96 pKa = 5.39 TNSSNFVAASKK107 pKa = 10.81 QLGNWLKK114 pKa = 10.8 VNLFKK119 pKa = 10.78 KK120 pKa = 10.46 VRR122 pKa = 11.84 LQGDD126 pKa = 3.72 VKK128 pKa = 10.62 DD129 pKa = 4.17 VHH131 pKa = 6.48 WNAHH135 pKa = 4.61 VSCPNQLVGVIAEE148 pKa = 4.04 FHH150 pKa = 6.57 RR151 pKa = 11.84 IEE153 pKa = 4.6 KK154 pKa = 8.92 GTLKK158 pKa = 9.22 TQYY161 pKa = 10.69 LRR163 pKa = 11.84 ALLSILNLYY172 pKa = 10.17 EE173 pKa = 5.06 SITIPTNPSLDD184 pKa = 4.13 TITKK188 pKa = 9.18 PSNVDD193 pKa = 2.94 QTQISSQEE201 pKa = 3.61 ILTALEE207 pKa = 3.91 TLGIDD212 pKa = 3.64 VAAVTNDD219 pKa = 3.42 FKK221 pKa = 11.55 FMNQSPTSHH230 pKa = 6.61 SSSSAGPNGQAVWYY244 pKa = 9.61 SHH246 pKa = 7.04 LDD248 pKa = 4.03 AKK250 pKa = 11.02 ALWQDD255 pKa = 3.2 EE256 pKa = 4.37 TLKK259 pKa = 10.26 MRR261 pKa = 11.84 FNSLATILEE270 pKa = 4.21 RR271 pKa = 11.84 EE272 pKa = 4.24 DD273 pKa = 4.87 LKK275 pKa = 10.62 TLLKK279 pKa = 10.62 EE280 pKa = 4.25 SAGLPKK286 pKa = 10.24 LQKK289 pKa = 10.74 ANRR292 pKa = 11.84 NKK294 pKa = 9.87 PNQPKK299 pKa = 7.62 QHH301 pKa = 6.72 KK302 pKa = 9.8 IYY304 pKa = 10.8 EE305 pKa = 4.55 KK306 pKa = 10.38 GNKK309 pKa = 7.49 TRR311 pKa = 11.84 VIAILDD317 pKa = 3.79 YY318 pKa = 8.79 FTQEE322 pKa = 3.55 ILTPFHH328 pKa = 7.19 DD329 pKa = 4.69 LVASILKK336 pKa = 9.65 KK337 pKa = 10.22 IEE339 pKa = 3.64 MDD341 pKa = 3.48 GTFDD345 pKa = 3.33 QDD347 pKa = 4.9 KK348 pKa = 9.12 IARR351 pKa = 11.84 WVKK354 pKa = 10.41 SKK356 pKa = 8.99 TAQSCVSLYY365 pKa = 10.91 SYY367 pKa = 11.43 DD368 pKa = 3.31 LTAATDD374 pKa = 4.0 RR375 pKa = 11.84 LPVWLQRR382 pKa = 11.84 RR383 pKa = 11.84 IIEE386 pKa = 4.17 CLIKK390 pKa = 10.01 IDD392 pKa = 4.24 NFGLNWQLLLTDD404 pKa = 4.36 RR405 pKa = 11.84 AFDD408 pKa = 3.74 NPIGEE413 pKa = 4.23 PVKK416 pKa = 10.86 YY417 pKa = 10.31 CVGQPMGAKK426 pKa = 9.91 SSFPMLGLTHH436 pKa = 6.91 HH437 pKa = 7.24 IIVQIAAKK445 pKa = 8.89 RR446 pKa = 11.84 TGFQGLFTEE455 pKa = 4.55 YY456 pKa = 10.75 VILGDD461 pKa = 5.11 DD462 pKa = 3.2 ICIADD467 pKa = 4.55 DD468 pKa = 4.45 LVAAKK473 pKa = 9.65 YY474 pKa = 10.3 KK475 pKa = 11.09 EE476 pKa = 3.88 MMIEE480 pKa = 4.14 LGLTISEE487 pKa = 4.52 HH488 pKa = 5.04 KK489 pKa = 9.66 TIVSTVEE496 pKa = 3.65 TSIDD500 pKa = 3.54 PVAEE504 pKa = 3.41 ICKK507 pKa = 9.69 RR508 pKa = 11.84 VFIGGVEE515 pKa = 4.09 VTPLPMKK522 pKa = 10.45 LAANVTEE529 pKa = 4.13 NNDD532 pKa = 3.06 LFFQFRR538 pKa = 11.84 EE539 pKa = 4.16 KK540 pKa = 10.72 LSEE543 pKa = 4.11 RR544 pKa = 11.84 GLIYY548 pKa = 10.97 NPADD552 pKa = 2.84 WKK554 pKa = 11.05 YY555 pKa = 10.29 FVAACVSNHH564 pKa = 6.15 RR565 pKa = 11.84 SLEE568 pKa = 4.11 KK569 pKa = 10.16 IGLYY573 pKa = 10.76 NSMPLWLSGFKK584 pKa = 10.16 QSLPIGTVGNNNFEE598 pKa = 4.53 DD599 pKa = 3.63 WLKK602 pKa = 11.45 AGMTEE607 pKa = 4.31 DD608 pKa = 3.71 YY609 pKa = 10.79 LRR611 pKa = 11.84 EE612 pKa = 3.84 VWTFTVLQEE621 pKa = 3.84 QFKK624 pKa = 10.71 RR625 pKa = 11.84 ISTLIQSAQDD635 pKa = 3.38 TFQTISKK642 pKa = 9.8 GIRR645 pKa = 11.84 LGDD648 pKa = 3.41 AVYY651 pKa = 10.1 IVPDD655 pKa = 3.55 TGIEE659 pKa = 4.09 LPVTKK664 pKa = 10.31 FDD666 pKa = 3.5 IQKK669 pKa = 8.83 MNEE672 pKa = 3.69 WNILNIPHH680 pKa = 7.67 PARR683 pKa = 11.84 FVMLGEE689 pKa = 4.25 VNRR692 pKa = 11.84 VATLFSQLSSIRR704 pKa = 11.84 GPSLMTKK711 pKa = 8.85 LTHH714 pKa = 7.19 NIVDD718 pKa = 3.59 NLKK721 pKa = 10.45 FSVLEE726 pKa = 4.62 MINDD730 pKa = 3.32 MDD732 pKa = 4.05 NYY734 pKa = 9.42 KK735 pKa = 10.89 ARR737 pKa = 11.84 VDD739 pKa = 3.65 RR740 pKa = 11.84 QLLEE744 pKa = 4.33 KK745 pKa = 9.62 MISNIRR751 pKa = 11.84 MIIRR755 pKa = 11.84 AEE757 pKa = 4.1 NKK759 pKa = 8.45 TVTYY763 pKa = 8.42 TVKK766 pKa = 9.76 LTPLGIVWVLRR777 pKa = 11.84 LSHH780 pKa = 6.98 LGSCTLFRR788 pKa = 11.84 STSAIPTTLRR798 pKa = 11.84 DD799 pKa = 3.44 AEE801 pKa = 4.41 LKK803 pKa = 9.79 FKK805 pKa = 10.88 RR806 pKa = 11.84 MNSGNSQIASIFDD819 pKa = 3.48 TT820 pKa = 4.34
Molecular weight: 92.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.172
IPC2_protein 8.185
IPC_protein 8.039
Toseland 8.639
ProMoST 8.624
Dawson 9.033
Bjellqvist 8.96
Wikipedia 9.121
Rodwell 9.224
Grimsley 8.916
Solomon 9.092
Lehninger 9.063
Nozaki 9.121
DTASelect 8.829
Thurlkill 8.975
EMBOSS 9.194
Sillero 9.151
Patrickios 4.787
IPC_peptide 9.092
IPC2_peptide 7.571
IPC2.peptide.svr19 7.616
Protein with the highest isoelectric point:
>tr|A0A166H5D4|A0A166H5D4_9VIRU Putative RNA-dependent RNA polymerase OS=Rhizoctonia oryzae-sativae mitovirus 1 OX=1837089 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.24 IINTTINNKK11 pKa = 8.87 TNTNFLSFSSGYY23 pKa = 9.77 SFSSFSPNATNSTTVKK39 pKa = 10.44 DD40 pKa = 3.89 LRR42 pKa = 11.84 QSIVPGINVVRR53 pKa = 11.84 NSTRR57 pKa = 11.84 LARR60 pKa = 11.84 SIALLTAYY68 pKa = 9.65 IEE70 pKa = 4.1 IQLRR74 pKa = 11.84 DD75 pKa = 3.73 LDD77 pKa = 3.94 DD78 pKa = 3.63 TSRR81 pKa = 11.84 MVLKK85 pKa = 10.75 QFMTEE90 pKa = 4.16 LEE92 pKa = 4.38 SMYY95 pKa = 9.51 HH96 pKa = 5.39 TNSSNFVAASKK107 pKa = 10.81 QLGNWLKK114 pKa = 10.8 VNLFKK119 pKa = 10.78 KK120 pKa = 10.46 VRR122 pKa = 11.84 LQGDD126 pKa = 3.72 VKK128 pKa = 10.62 DD129 pKa = 4.17 VHH131 pKa = 6.48 WNAHH135 pKa = 4.61 VSCPNQLVGVIAEE148 pKa = 4.04 FHH150 pKa = 6.57 RR151 pKa = 11.84 IEE153 pKa = 4.6 KK154 pKa = 8.92 GTLKK158 pKa = 9.22 TQYY161 pKa = 10.69 LRR163 pKa = 11.84 ALLSILNLYY172 pKa = 10.17 EE173 pKa = 5.06 SITIPTNPSLDD184 pKa = 4.13 TITKK188 pKa = 9.18 PSNVDD193 pKa = 2.94 QTQISSQEE201 pKa = 3.61 ILTALEE207 pKa = 3.91 TLGIDD212 pKa = 3.64 VAAVTNDD219 pKa = 3.42 FKK221 pKa = 11.55 FMNQSPTSHH230 pKa = 6.61 SSSSAGPNGQAVWYY244 pKa = 9.61 SHH246 pKa = 7.04 LDD248 pKa = 4.03 AKK250 pKa = 11.02 ALWQDD255 pKa = 3.2 EE256 pKa = 4.37 TLKK259 pKa = 10.26 MRR261 pKa = 11.84 FNSLATILEE270 pKa = 4.21 RR271 pKa = 11.84 EE272 pKa = 4.24 DD273 pKa = 4.87 LKK275 pKa = 10.62 TLLKK279 pKa = 10.62 EE280 pKa = 4.25 SAGLPKK286 pKa = 10.24 LQKK289 pKa = 10.74 ANRR292 pKa = 11.84 NKK294 pKa = 9.87 PNQPKK299 pKa = 7.62 QHH301 pKa = 6.72 KK302 pKa = 9.8 IYY304 pKa = 10.8 EE305 pKa = 4.55 KK306 pKa = 10.38 GNKK309 pKa = 7.49 TRR311 pKa = 11.84 VIAILDD317 pKa = 3.79 YY318 pKa = 8.79 FTQEE322 pKa = 3.55 ILTPFHH328 pKa = 7.19 DD329 pKa = 4.69 LVASILKK336 pKa = 9.65 KK337 pKa = 10.22 IEE339 pKa = 3.64 MDD341 pKa = 3.48 GTFDD345 pKa = 3.33 QDD347 pKa = 4.9 KK348 pKa = 9.12 IARR351 pKa = 11.84 WVKK354 pKa = 10.41 SKK356 pKa = 8.99 TAQSCVSLYY365 pKa = 10.91 SYY367 pKa = 11.43 DD368 pKa = 3.31 LTAATDD374 pKa = 4.0 RR375 pKa = 11.84 LPVWLQRR382 pKa = 11.84 RR383 pKa = 11.84 IIEE386 pKa = 4.17 CLIKK390 pKa = 10.01 IDD392 pKa = 4.24 NFGLNWQLLLTDD404 pKa = 4.36 RR405 pKa = 11.84 AFDD408 pKa = 3.74 NPIGEE413 pKa = 4.23 PVKK416 pKa = 10.86 YY417 pKa = 10.31 CVGQPMGAKK426 pKa = 9.91 SSFPMLGLTHH436 pKa = 6.91 HH437 pKa = 7.24 IIVQIAAKK445 pKa = 8.89 RR446 pKa = 11.84 TGFQGLFTEE455 pKa = 4.55 YY456 pKa = 10.75 VILGDD461 pKa = 5.11 DD462 pKa = 3.2 ICIADD467 pKa = 4.55 DD468 pKa = 4.45 LVAAKK473 pKa = 9.65 YY474 pKa = 10.3 KK475 pKa = 11.09 EE476 pKa = 3.88 MMIEE480 pKa = 4.14 LGLTISEE487 pKa = 4.52 HH488 pKa = 5.04 KK489 pKa = 9.66 TIVSTVEE496 pKa = 3.65 TSIDD500 pKa = 3.54 PVAEE504 pKa = 3.41 ICKK507 pKa = 9.69 RR508 pKa = 11.84 VFIGGVEE515 pKa = 4.09 VTPLPMKK522 pKa = 10.45 LAANVTEE529 pKa = 4.13 NNDD532 pKa = 3.06 LFFQFRR538 pKa = 11.84 EE539 pKa = 4.16 KK540 pKa = 10.72 LSEE543 pKa = 4.11 RR544 pKa = 11.84 GLIYY548 pKa = 10.97 NPADD552 pKa = 2.84 WKK554 pKa = 11.05 YY555 pKa = 10.29 FVAACVSNHH564 pKa = 6.15 RR565 pKa = 11.84 SLEE568 pKa = 4.11 KK569 pKa = 10.16 IGLYY573 pKa = 10.76 NSMPLWLSGFKK584 pKa = 10.16 QSLPIGTVGNNNFEE598 pKa = 4.53 DD599 pKa = 3.63 WLKK602 pKa = 11.45 AGMTEE607 pKa = 4.31 DD608 pKa = 3.71 YY609 pKa = 10.79 LRR611 pKa = 11.84 EE612 pKa = 3.84 VWTFTVLQEE621 pKa = 3.84 QFKK624 pKa = 10.71 RR625 pKa = 11.84 ISTLIQSAQDD635 pKa = 3.38 TFQTISKK642 pKa = 9.8 GIRR645 pKa = 11.84 LGDD648 pKa = 3.41 AVYY651 pKa = 10.1 IVPDD655 pKa = 3.55 TGIEE659 pKa = 4.09 LPVTKK664 pKa = 10.31 FDD666 pKa = 3.5 IQKK669 pKa = 8.83 MNEE672 pKa = 3.69 WNILNIPHH680 pKa = 7.67 PARR683 pKa = 11.84 FVMLGEE689 pKa = 4.25 VNRR692 pKa = 11.84 VATLFSQLSSIRR704 pKa = 11.84 GPSLMTKK711 pKa = 8.85 LTHH714 pKa = 7.19 NIVDD718 pKa = 3.59 NLKK721 pKa = 10.45 FSVLEE726 pKa = 4.62 MINDD730 pKa = 3.32 MDD732 pKa = 4.05 NYY734 pKa = 9.42 KK735 pKa = 10.89 ARR737 pKa = 11.84 VDD739 pKa = 3.65 RR740 pKa = 11.84 QLLEE744 pKa = 4.33 KK745 pKa = 9.62 MISNIRR751 pKa = 11.84 MIIRR755 pKa = 11.84 AEE757 pKa = 4.1 NKK759 pKa = 8.45 TVTYY763 pKa = 8.42 TVKK766 pKa = 9.76 LTPLGIVWVLRR777 pKa = 11.84 LSHH780 pKa = 6.98 LGSCTLFRR788 pKa = 11.84 STSAIPTTLRR798 pKa = 11.84 DD799 pKa = 3.44 AEE801 pKa = 4.41 LKK803 pKa = 9.79 FKK805 pKa = 10.88 RR806 pKa = 11.84 MNSGNSQIASIFDD819 pKa = 3.48 TT820 pKa = 4.34
Molecular weight: 92.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.172
IPC2_protein 8.185
IPC_protein 8.039
Toseland 8.639
ProMoST 8.624
Dawson 9.033
Bjellqvist 8.96
Wikipedia 9.121
Rodwell 9.224
Grimsley 8.916
Solomon 9.092
Lehninger 9.063
Nozaki 9.121
DTASelect 8.829
Thurlkill 8.975
EMBOSS 9.194
Sillero 9.151
Patrickios 4.787
IPC_peptide 9.092
IPC2_peptide 7.571
IPC2.peptide.svr19 7.616
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
820
820
820
820.0
92.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.854 ± 0.0
0.976 ± 0.0
5.122 ± 0.0
4.756 ± 0.0
4.39 ± 0.0
4.512 ± 0.0
1.829 ± 0.0
7.927 ± 0.0
6.707 ± 0.0
10.732 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.683 ± 0.0
6.098 ± 0.0
3.659 ± 0.0
4.268 ± 0.0
4.512 ± 0.0
7.805 ± 0.0
7.805 ± 0.0
6.341 ± 0.0
1.585 ± 0.0
2.439 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here