Neorhizobium sp. JUb45

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Neorhizobium; unclassified Neorhizobium

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3ADZ1|A0A4R3ADZ1_9RHIZ 4-phosphoerythronate dehydrogenase (FAD-dependent) OS=Neorhizobium sp. JUb45 OX=2485113 GN=EDF70_102297 PE=4 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.83TCLKK58 pKa = 9.4IGGYY62 pKa = 9.81VRR64 pKa = 11.84TQIGGRR70 pKa = 11.84SDD72 pKa = 3.77FDD74 pKa = 3.63IDD76 pKa = 3.88GVGRR80 pKa = 11.84VQDD83 pKa = 3.51GGYY86 pKa = 7.86ITNTRR91 pKa = 11.84GYY93 pKa = 8.78LTISAKK99 pKa = 10.43SDD101 pKa = 3.59TEE103 pKa = 4.28LGTLGSSISMEE114 pKa = 4.06ADD116 pKa = 3.29TNGGSQDD123 pKa = 3.51FFVDD127 pKa = 3.58EE128 pKa = 5.27AIITLGGLQVGYY140 pKa = 9.82FYY142 pKa = 11.29NYY144 pKa = 9.56FDD146 pKa = 4.02NGLPGEE152 pKa = 4.55TVLSDD157 pKa = 3.5GGPIAGANYY166 pKa = 9.98IGGDD170 pKa = 3.56TLNNSIRR177 pKa = 11.84YY178 pKa = 6.42TFDD181 pKa = 2.88GGAFQAYY188 pKa = 8.59IQGDD192 pKa = 3.88FFDD195 pKa = 5.4SQDD198 pKa = 3.04GWKK201 pKa = 9.16TDD203 pKa = 3.49VNDD206 pKa = 3.6EE207 pKa = 4.4EE208 pKa = 4.82IGINAMIAGKK218 pKa = 10.15FGGVAIDD225 pKa = 4.15LFGAYY230 pKa = 9.64EE231 pKa = 4.17FDD233 pKa = 4.19NEE235 pKa = 4.57DD236 pKa = 3.19GVIASTLAAEE246 pKa = 4.96IGPGTLGVFGVYY258 pKa = 10.21SFGPSRR264 pKa = 11.84YY265 pKa = 7.94WDD267 pKa = 3.78FSEE270 pKa = 3.92WSIGAEE276 pKa = 3.84YY277 pKa = 10.27AIAVSDD283 pKa = 3.98KK284 pKa = 11.45LKK286 pKa = 9.26ITPGAQYY293 pKa = 9.74WDD295 pKa = 4.22NILPIGSDD303 pKa = 3.85YY304 pKa = 11.46EE305 pKa = 4.47GGSAWRR311 pKa = 11.84VGVTADD317 pKa = 3.48YY318 pKa = 9.67TITTNLTAKK327 pKa = 9.65ATVNYY332 pKa = 9.57TDD334 pKa = 3.23VDD336 pKa = 3.64VDD338 pKa = 4.01FGGVGGGDD346 pKa = 2.73RR347 pKa = 11.84WDD349 pKa = 3.45GFVRR353 pKa = 11.84LQRR356 pKa = 11.84SFF358 pKa = 3.42

Molecular weight: 37.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3A5P7|A0A4R3A5P7_9RHIZ Ammonium transporter OS=Neorhizobium sp. JUb45 OX=2485113 GN=EDF70_105288 PE=3 SV=1
MM1 pKa = 7.56TSLINQIVSGVVEE14 pKa = 4.42SVVKK18 pKa = 10.27EE19 pKa = 3.99LLGKK23 pKa = 9.37SHH25 pKa = 6.35GRR27 pKa = 11.84TASKK31 pKa = 10.08RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.74RR35 pKa = 11.84QAKK38 pKa = 8.99AATRR42 pKa = 11.84STTTGRR48 pKa = 11.84FTRR51 pKa = 11.84KK52 pKa = 5.19TTKK55 pKa = 10.27KK56 pKa = 9.33AAKK59 pKa = 8.75PARR62 pKa = 11.84KK63 pKa = 8.73QVSRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84TAVSRR74 pKa = 11.84SKK76 pKa = 10.59QRR78 pKa = 11.84SRR80 pKa = 3.52

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5878

0

5878

1828676

27

9415

311.1

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.784 ± 0.043

0.743 ± 0.011

5.784 ± 0.032

5.677 ± 0.038

3.901 ± 0.021

8.351 ± 0.04

1.981 ± 0.019

5.767 ± 0.021

3.753 ± 0.032

9.812 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.021

2.947 ± 0.026

4.839 ± 0.026

3.196 ± 0.02

6.397 ± 0.039

5.916 ± 0.03

5.628 ± 0.054

7.328 ± 0.035

1.251 ± 0.015

2.292 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski