Phaeocystis globosa virus 12T

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prymnesiovirus; unclassified Prymnesiovirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 439 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8DH94|G8DH94_9PHYC DNA polymerase III OS=Phaeocystis globosa virus 12T OX=755273 GN=PGAG_00400 PE=4 SV=1
MM1 pKa = 6.91TTMTNAQITSLKK13 pKa = 10.21NRR15 pKa = 11.84LRR17 pKa = 11.84THH19 pKa = 6.74FDD21 pKa = 2.87MDD23 pKa = 3.6RR24 pKa = 11.84VLEE27 pKa = 4.29TFSEE31 pKa = 4.71EE32 pKa = 3.57VWEE35 pKa = 4.1NNNYY39 pKa = 9.76GEE41 pKa = 4.47AYY43 pKa = 9.77EE44 pKa = 4.47NLTEE48 pKa = 4.18EE49 pKa = 4.88KK50 pKa = 10.28FMKK53 pKa = 10.49YY54 pKa = 9.35FAEE57 pKa = 4.35AMLNPMEE64 pKa = 5.09EE65 pKa = 3.95EE66 pKa = 4.65DD67 pKa = 4.15EE68 pKa = 4.93DD69 pKa = 4.69VEE71 pKa = 6.51KK72 pKa = 10.73PMCWEE77 pKa = 4.6CGVSRR82 pKa = 11.84CDD84 pKa = 4.44DD85 pKa = 3.52IEE87 pKa = 4.63FSICEE92 pKa = 3.95CCMNKK97 pKa = 9.81PCIDD101 pKa = 4.53DD102 pKa = 5.58DD103 pKa = 4.28EE104 pKa = 5.21CCPEE108 pKa = 3.86VNMDD112 pKa = 4.68DD113 pKa = 4.2EE114 pKa = 4.57EE115 pKa = 4.25

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8DI06|G8DI06_9PHYC Uncharacterized protein OS=Phaeocystis globosa virus 12T OX=755273 GN=PGAG_00227 PE=4 SV=1
MM1 pKa = 7.06TLAMTNLQNGGRR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84SSKK19 pKa = 10.66AQRR22 pKa = 11.84KK23 pKa = 6.0SQRR26 pKa = 11.84RR27 pKa = 11.84GQKK30 pKa = 8.94KK31 pKa = 9.64GGMAGIVTTANAVPASLLLLNQYY54 pKa = 10.66LKK56 pKa = 10.9ARR58 pKa = 11.84KK59 pKa = 8.79SRR61 pKa = 11.84KK62 pKa = 9.18ASKK65 pKa = 10.44KK66 pKa = 8.67SAKK69 pKa = 8.71KK70 pKa = 7.47TAKK73 pKa = 9.76KK74 pKa = 10.06SRR76 pKa = 11.84RR77 pKa = 11.84SSRR80 pKa = 11.84KK81 pKa = 8.81

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

439

0

439

137610

49

3739

313.5

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.586 ± 0.126

1.495 ± 0.075

6.624 ± 0.117

6.16 ± 0.163

4.307 ± 0.097

5.072 ± 0.227

1.886 ± 0.074

8.6 ± 0.122

9.15 ± 0.293

8.119 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.082

8.602 ± 0.157

3.172 ± 0.07

2.814 ± 0.065

3.002 ± 0.084

6.678 ± 0.147

6.295 ± 0.129

5.327 ± 0.122

0.762 ± 0.044

4.822 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski