Amino acid dipepetide frequency for Psychrobacillus phage Perkons

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.751AlaAla: 0.751 ± 0.214
0.325AlaCys: 0.325 ± 0.09
2.502AlaAsp: 2.502 ± 0.234
2.627AlaGlu: 2.627 ± 0.27
2.052AlaPhe: 2.052 ± 0.273
2.377AlaGly: 2.377 ± 0.309
0.726AlaHis: 0.726 ± 0.144
3.528AlaIle: 3.528 ± 0.282
4.654AlaLys: 4.654 ± 0.449
3.778AlaLeu: 3.778 ± 0.487
0.901AlaMet: 0.901 ± 0.197
2.752AlaAsn: 2.752 ± 0.239
0.55AlaPro: 0.55 ± 0.109
1.501AlaGln: 1.501 ± 0.265
1.526AlaArg: 1.526 ± 0.201
3.053AlaSer: 3.053 ± 0.4
2.752AlaThr: 2.752 ± 0.397
2.077AlaVal: 2.077 ± 0.187
0.525AlaTrp: 0.525 ± 0.114
2.077AlaTyr: 2.077 ± 0.253
0.0AlaXaa: 0.0 ± 0.0
Cys
0.425CysAla: 0.425 ± 0.142
0.025CysCys: 0.025 ± 0.03
0.676CysAsp: 0.676 ± 0.15
0.926CysGlu: 0.926 ± 0.184
0.225CysPhe: 0.225 ± 0.072
0.751CysGly: 0.751 ± 0.144
0.2CysHis: 0.2 ± 0.064
0.701CysIle: 0.701 ± 0.139
0.751CysLys: 0.751 ± 0.138
0.626CysLeu: 0.626 ± 0.141
0.375CysMet: 0.375 ± 0.114
0.801CysAsn: 0.801 ± 0.141
0.25CysPro: 0.25 ± 0.094
0.35CysGln: 0.35 ± 0.087
0.225CysArg: 0.225 ± 0.075
0.575CysSer: 0.575 ± 0.103
0.5CysThr: 0.5 ± 0.123
0.425CysVal: 0.425 ± 0.097
0.1CysTrp: 0.1 ± 0.054
0.275CysTyr: 0.275 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
2.602AspAla: 2.602 ± 0.352
0.525AspCys: 0.525 ± 0.126
4.228AspAsp: 4.228 ± 0.309
6.455AspGlu: 6.455 ± 0.361
3.653AspPhe: 3.653 ± 0.313
4.203AspGly: 4.203 ± 0.324
0.801AspHis: 0.801 ± 0.134
6.455AspIle: 6.455 ± 0.488
5.855AspLys: 5.855 ± 0.419
5.079AspLeu: 5.079 ± 0.306
1.827AspMet: 1.827 ± 0.214
4.354AspAsn: 4.354 ± 0.284
0.801AspPro: 0.801 ± 0.157
1.051AspGln: 1.051 ± 0.18
2.252AspArg: 2.252 ± 0.217
4.003AspSer: 4.003 ± 0.382
3.403AspThr: 3.403 ± 0.33
4.153AspVal: 4.153 ± 0.327
0.901AspTrp: 0.901 ± 0.165
3.403AspTyr: 3.403 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
3.027GluAla: 3.027 ± 0.337
0.626GluCys: 0.626 ± 0.12
5.63GluAsp: 5.63 ± 0.435
6.731GluGlu: 6.731 ± 0.585
3.878GluPhe: 3.878 ± 0.336
3.403GluGly: 3.403 ± 0.354
1.451GluHis: 1.451 ± 0.182
7.231GluIle: 7.231 ± 0.63
7.006GluLys: 7.006 ± 0.418
9.433GluLeu: 9.433 ± 0.485
2.452GluMet: 2.452 ± 0.253
5.254GluAsn: 5.254 ± 0.369
1.176GluPro: 1.176 ± 0.175
3.027GluGln: 3.027 ± 0.263
3.153GluArg: 3.153 ± 0.287
4.829GluSer: 4.829 ± 0.362
3.653GluThr: 3.653 ± 0.367
6.03GluVal: 6.03 ± 0.454
1.276GluTrp: 1.276 ± 0.186
4.554GluTyr: 4.554 ± 0.371
0.0GluXaa: 0.0 ± 0.0
Phe
1.601PheAla: 1.601 ± 0.227
0.5PheCys: 0.5 ± 0.112
3.428PheAsp: 3.428 ± 0.261
4.053PheGlu: 4.053 ± 0.31
1.301PhePhe: 1.301 ± 0.193
2.352PheGly: 2.352 ± 0.25
0.876PheHis: 0.876 ± 0.155
4.279PheIle: 4.279 ± 0.377
4.028PheLys: 4.028 ± 0.318
3.128PheLeu: 3.128 ± 0.31
0.901PheMet: 0.901 ± 0.155
3.228PheAsn: 3.228 ± 0.217
1.001PhePro: 1.001 ± 0.169
1.301PheGln: 1.301 ± 0.172
1.601PheArg: 1.601 ± 0.255
2.302PheSer: 2.302 ± 0.218
2.777PheThr: 2.777 ± 0.285
2.527PheVal: 2.527 ± 0.223
0.275PheTrp: 0.275 ± 0.084
2.302PheTyr: 2.302 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
1.877GlyAla: 1.877 ± 0.313
0.425GlyCys: 0.425 ± 0.102
3.027GlyAsp: 3.027 ± 0.209
4.203GlyGlu: 4.203 ± 0.319
2.902GlyPhe: 2.902 ± 0.306
3.153GlyGly: 3.153 ± 0.293
0.826GlyHis: 0.826 ± 0.138
4.178GlyIle: 4.178 ± 0.316
4.579GlyLys: 4.579 ± 0.375
4.754GlyLeu: 4.754 ± 0.297
1.827GlyMet: 1.827 ± 0.237
3.528GlyAsn: 3.528 ± 0.308
0.025GlyPro: 0.025 ± 0.028
1.451GlyGln: 1.451 ± 0.174
1.701GlyArg: 1.701 ± 0.224
3.828GlySer: 3.828 ± 0.368
3.228GlyThr: 3.228 ± 0.292
3.503GlyVal: 3.503 ± 0.262
0.851GlyTrp: 0.851 ± 0.166
3.103GlyTyr: 3.103 ± 0.253
0.0GlyXaa: 0.0 ± 0.0
His
0.726HisAla: 0.726 ± 0.135
0.35HisCys: 0.35 ± 0.092
1.176HisAsp: 1.176 ± 0.152
1.551HisGlu: 1.551 ± 0.207
0.976HisPhe: 0.976 ± 0.144
0.776HisGly: 0.776 ± 0.128
0.425HisHis: 0.425 ± 0.112
1.576HisIle: 1.576 ± 0.202
1.451HisLys: 1.451 ± 0.193
1.426HisLeu: 1.426 ± 0.171
0.425HisMet: 0.425 ± 0.099
1.226HisAsn: 1.226 ± 0.155
0.425HisPro: 0.425 ± 0.101
0.525HisGln: 0.525 ± 0.108
0.475HisArg: 0.475 ± 0.108
1.226HisSer: 1.226 ± 0.185
0.976HisThr: 0.976 ± 0.145
0.951HisVal: 0.951 ± 0.171
0.25HisTrp: 0.25 ± 0.1
1.076HisTyr: 1.076 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
3.203IleAla: 3.203 ± 0.338
0.751IleCys: 0.751 ± 0.156
6.055IleAsp: 6.055 ± 0.422
7.907IleGlu: 7.907 ± 0.49
3.002IlePhe: 3.002 ± 0.291
4.504IleGly: 4.504 ± 0.362
1.676IleHis: 1.676 ± 0.194
6.03IleIle: 6.03 ± 0.439
8.432IleLys: 8.432 ± 0.412
5.354IleLeu: 5.354 ± 0.407
1.776IleMet: 1.776 ± 0.266
7.156IleAsn: 7.156 ± 0.465
2.302IlePro: 2.302 ± 0.267
2.402IleGln: 2.402 ± 0.276
2.977IleArg: 2.977 ± 0.265
5.054IleSer: 5.054 ± 0.373
5.129IleThr: 5.129 ± 0.457
4.679IleVal: 4.679 ± 0.416
0.5IleTrp: 0.5 ± 0.11
3.228IleTyr: 3.228 ± 0.289
0.0IleXaa: 0.0 ± 0.0
Lys
4.153LysAla: 4.153 ± 0.358
0.776LysCys: 0.776 ± 0.159
5.63LysAsp: 5.63 ± 0.342
7.831LysGlu: 7.831 ± 0.455
3.678LysPhe: 3.678 ± 0.308
4.829LysGly: 4.829 ± 0.386
1.726LysHis: 1.726 ± 0.217
6.831LysIle: 6.831 ± 0.401
6.831LysLys: 6.831 ± 0.477
9.082LysLeu: 9.082 ± 0.548
3.002LysMet: 3.002 ± 0.25
5.68LysAsn: 5.68 ± 0.327
1.977LysPro: 1.977 ± 0.256
3.678LysGln: 3.678 ± 0.369
3.002LysArg: 3.002 ± 0.281
5.329LysSer: 5.329 ± 0.447
3.953LysThr: 3.953 ± 0.255
6.43LysVal: 6.43 ± 0.407
1.026LysTrp: 1.026 ± 0.155
4.704LysTyr: 4.704 ± 0.352
0.0LysXaa: 0.0 ± 0.0
Leu
3.953LeuAla: 3.953 ± 0.358
0.776LeuCys: 0.776 ± 0.138
5.454LeuAsp: 5.454 ± 0.367
7.381LeuGlu: 7.381 ± 0.392
3.678LeuPhe: 3.678 ± 0.348
4.178LeuGly: 4.178 ± 0.305
1.651LeuHis: 1.651 ± 0.214
7.131LeuIle: 7.131 ± 0.439
6.931LeuLys: 6.931 ± 0.458
6.455LeuLeu: 6.455 ± 0.386
2.002LeuMet: 2.002 ± 0.182
8.007LeuAsn: 8.007 ± 0.708
2.027LeuPro: 2.027 ± 0.214
2.752LeuGln: 2.752 ± 0.253
2.927LeuArg: 2.927 ± 0.297
5.605LeuSer: 5.605 ± 0.407
5.68LeuThr: 5.68 ± 0.44
4.379LeuVal: 4.379 ± 0.387
0.801LeuTrp: 0.801 ± 0.129
4.003LeuTyr: 4.003 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
1.226MetAla: 1.226 ± 0.178
0.325MetCys: 0.325 ± 0.106
1.751MetAsp: 1.751 ± 0.207
2.452MetGlu: 2.452 ± 0.246
0.751MetPhe: 0.751 ± 0.126
0.901MetGly: 0.901 ± 0.156
0.4MetHis: 0.4 ± 0.092
2.027MetIle: 2.027 ± 0.218
3.228MetLys: 3.228 ± 0.286
1.852MetLeu: 1.852 ± 0.236
0.626MetMet: 0.626 ± 0.137
2.277MetAsn: 2.277 ± 0.245
0.325MetPro: 0.325 ± 0.094
0.726MetGln: 0.726 ± 0.117
0.951MetArg: 0.951 ± 0.144
2.227MetSer: 2.227 ± 0.226
1.927MetThr: 1.927 ± 0.175
1.551MetVal: 1.551 ± 0.201
0.35MetTrp: 0.35 ± 0.099
0.926MetTyr: 0.926 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
3.128AsnAla: 3.128 ± 0.455
0.45AsnCys: 0.45 ± 0.111
5.029AsnAsp: 5.029 ± 0.356
6.03AsnGlu: 6.03 ± 0.459
2.977AsnPhe: 2.977 ± 0.237
5.079AsnGly: 5.079 ± 0.351
1.401AsnHis: 1.401 ± 0.164
5.004AsnIle: 5.004 ± 0.361
6.355AsnLys: 6.355 ± 0.396
5.204AsnLeu: 5.204 ± 0.388
1.877AsnMet: 1.877 ± 0.21
4.829AsnAsn: 4.829 ± 0.31
1.726AsnPro: 1.726 ± 0.195
2.027AsnGln: 2.027 ± 0.213
2.927AsnArg: 2.927 ± 0.257
4.203AsnSer: 4.203 ± 0.384
4.354AsnThr: 4.354 ± 0.331
4.654AsnVal: 4.654 ± 0.33
0.776AsnTrp: 0.776 ± 0.139
3.378AsnTyr: 3.378 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
0.751ProAla: 0.751 ± 0.141
0.275ProCys: 0.275 ± 0.084
0.901ProAsp: 0.901 ± 0.158
1.276ProGlu: 1.276 ± 0.187
1.001ProPhe: 1.001 ± 0.174
0.0ProGly: 0.0 ± 0.0
0.375ProHis: 0.375 ± 0.117
1.952ProIle: 1.952 ± 0.245
1.977ProLys: 1.977 ± 0.257
1.576ProLeu: 1.576 ± 0.218
0.5ProMet: 0.5 ± 0.096
1.751ProAsn: 1.751 ± 0.196
0.375ProPro: 0.375 ± 0.119
0.701ProGln: 0.701 ± 0.127
0.5ProArg: 0.5 ± 0.134
1.401ProSer: 1.401 ± 0.153
1.801ProThr: 1.801 ± 0.223
1.351ProVal: 1.351 ± 0.174
0.15ProTrp: 0.15 ± 0.061
0.876ProTyr: 0.876 ± 0.166
0.0ProXaa: 0.0 ± 0.0
Gln
1.827GlnAla: 1.827 ± 0.287
0.325GlnCys: 0.325 ± 0.075
1.551GlnAsp: 1.551 ± 0.222
2.152GlnGlu: 2.152 ± 0.217
1.501GlnPhe: 1.501 ± 0.221
1.301GlnGly: 1.301 ± 0.177
0.35GlnHis: 0.35 ± 0.095
2.452GlnIle: 2.452 ± 0.255
2.502GlnLys: 2.502 ± 0.284
3.828GlnLeu: 3.828 ± 0.306
1.026GlnMet: 1.026 ± 0.15
1.827GlnAsn: 1.827 ± 0.215
0.776GlnPro: 0.776 ± 0.117
1.251GlnGln: 1.251 ± 0.209
1.326GlnArg: 1.326 ± 0.202
2.202GlnSer: 2.202 ± 0.292
1.701GlnThr: 1.701 ± 0.209
2.377GlnVal: 2.377 ± 0.225
0.3GlnTrp: 0.3 ± 0.068
1.526GlnTyr: 1.526 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
1.276ArgAla: 1.276 ± 0.184
0.4ArgCys: 0.4 ± 0.118
2.252ArgAsp: 2.252 ± 0.255
2.702ArgGlu: 2.702 ± 0.306
1.551ArgPhe: 1.551 ± 0.207
1.927ArgGly: 1.927 ± 0.229
0.626ArgHis: 0.626 ± 0.149
3.128ArgIle: 3.128 ± 0.31
3.278ArgLys: 3.278 ± 0.301
3.478ArgLeu: 3.478 ± 0.303
1.126ArgMet: 1.126 ± 0.185
2.377ArgAsn: 2.377 ± 0.261
0.55ArgPro: 0.55 ± 0.106
1.051ArgGln: 1.051 ± 0.188
1.126ArgArg: 1.126 ± 0.191
2.252ArgSer: 2.252 ± 0.247
1.952ArgThr: 1.952 ± 0.24
1.776ArgVal: 1.776 ± 0.22
0.525ArgTrp: 0.525 ± 0.114
1.701ArgTyr: 1.701 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
2.727SerAla: 2.727 ± 0.315
0.6SerCys: 0.6 ± 0.12
4.128SerAsp: 4.128 ± 0.342
5.404SerGlu: 5.404 ± 0.332
2.952SerPhe: 2.952 ± 0.331
3.628SerGly: 3.628 ± 0.353
1.326SerHis: 1.326 ± 0.187
5.379SerIle: 5.379 ± 0.384
5.88SerLys: 5.88 ± 0.36
5.655SerLeu: 5.655 ± 0.429
1.501SerMet: 1.501 ± 0.175
4.604SerAsn: 4.604 ± 0.335
1.476SerPro: 1.476 ± 0.184
2.002SerGln: 2.002 ± 0.208
2.227SerArg: 2.227 ± 0.237
4.354SerSer: 4.354 ± 0.432
3.403SerThr: 3.403 ± 0.327
3.803SerVal: 3.803 ± 0.347
0.701SerTrp: 0.701 ± 0.169
3.228SerTyr: 3.228 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
2.177ThrAla: 2.177 ± 0.311
0.4ThrCys: 0.4 ± 0.099
3.753ThrAsp: 3.753 ± 0.294
4.804ThrGlu: 4.804 ± 0.719
2.527ThrPhe: 2.527 ± 0.198
3.328ThrGly: 3.328 ± 0.371
1.026ThrHis: 1.026 ± 0.156
4.504ThrIle: 4.504 ± 0.364
5.029ThrLys: 5.029 ± 0.345
5.229ThrLeu: 5.229 ± 0.341
1.326ThrMet: 1.326 ± 0.177
3.328ThrAsn: 3.328 ± 0.29
1.476ThrPro: 1.476 ± 0.238
1.877ThrGln: 1.877 ± 0.207
1.601ThrArg: 1.601 ± 0.24
4.304ThrSer: 4.304 ± 0.386
2.852ThrThr: 2.852 ± 0.331
3.503ThrVal: 3.503 ± 0.316
0.475ThrTrp: 0.475 ± 0.118
3.027ThrTyr: 3.027 ± 0.252
0.0ThrXaa: 0.0 ± 0.0
Val
3.228ValAla: 3.228 ± 0.331
0.525ValCys: 0.525 ± 0.122
4.279ValAsp: 4.279 ± 0.31
4.654ValGlu: 4.654 ± 0.363
2.402ValPhe: 2.402 ± 0.297
3.378ValGly: 3.378 ± 0.273
0.951ValHis: 0.951 ± 0.155
4.854ValIle: 4.854 ± 0.364
5.98ValLys: 5.98 ± 0.387
4.629ValLeu: 4.629 ± 0.32
1.676ValMet: 1.676 ± 0.192
4.279ValAsn: 4.279 ± 0.313
1.251ValPro: 1.251 ± 0.165
2.202ValGln: 2.202 ± 0.231
2.427ValArg: 2.427 ± 0.273
4.379ValSer: 4.379 ± 0.383
3.328ValThr: 3.328 ± 0.312
3.678ValVal: 3.678 ± 0.385
0.575ValTrp: 0.575 ± 0.116
2.452ValTyr: 2.452 ± 0.25
0.0ValXaa: 0.0 ± 0.0
Trp
0.55TrpAla: 0.55 ± 0.128
0.075TrpCys: 0.075 ± 0.045
0.826TrpAsp: 0.826 ± 0.165
0.926TrpGlu: 0.926 ± 0.141
0.525TrpPhe: 0.525 ± 0.113
0.4TrpGly: 0.4 ± 0.106
0.15TrpHis: 0.15 ± 0.057
1.001TrpIle: 1.001 ± 0.171
1.176TrpLys: 1.176 ± 0.175
0.926TrpLeu: 0.926 ± 0.163
0.35TrpMet: 0.35 ± 0.084
1.001TrpAsn: 1.001 ± 0.185
0.0TrpPro: 0.0 ± 0.0
0.25TrpGln: 0.25 ± 0.079
0.425TrpArg: 0.425 ± 0.1
0.676TrpSer: 0.676 ± 0.141
0.701TrpThr: 0.701 ± 0.151
0.55TrpVal: 0.55 ± 0.108
0.1TrpTrp: 0.1 ± 0.05
0.375TrpTyr: 0.375 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.827TyrAla: 1.827 ± 0.214
0.726TyrCys: 0.726 ± 0.135
3.728TyrAsp: 3.728 ± 0.303
3.778TyrGlu: 3.778 ± 0.302
2.227TyrPhe: 2.227 ± 0.252
2.502TyrGly: 2.502 ± 0.278
1.026TyrHis: 1.026 ± 0.154
3.878TyrIle: 3.878 ± 0.358
4.228TyrLys: 4.228 ± 0.313
4.279TyrLeu: 4.279 ± 0.344
1.276TyrMet: 1.276 ± 0.209
3.153TyrAsn: 3.153 ± 0.275
1.001TyrPro: 1.001 ± 0.188
1.977TyrGln: 1.977 ± 0.195
1.701TyrArg: 1.701 ± 0.202
3.178TyrSer: 3.178 ± 0.263
2.477TyrThr: 2.477 ± 0.248
2.727TyrVal: 2.727 ± 0.299
0.525TyrTrp: 0.525 ± 0.107
2.502TyrTyr: 2.502 ± 0.283
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 217 proteins (39968 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski