uncultured phage_MedDCM-OCT-S46-C10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Foussvirus; Foussvirus S46C10

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PDZ4|A0A6S4PDZ4_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S46-C10 OX=2741074 PE=4 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 7.35TGNGNTTAYY17 pKa = 10.02SIPYY21 pKa = 9.41SYY23 pKa = 10.68RR24 pKa = 11.84DD25 pKa = 3.51PADD28 pKa = 4.47LIVTINGVATTSYY41 pKa = 9.57TLNSAGSTLTFDD53 pKa = 3.73TAPANSSAIEE63 pKa = 3.91IRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 8.21TSQTSRR73 pKa = 11.84LTDD76 pKa = 3.47YY77 pKa = 11.3AAGSVLTEE85 pKa = 4.12NDD87 pKa = 3.84LDD89 pKa = 4.08TDD91 pKa = 3.79STQAFFMSQEE101 pKa = 4.56AIDD104 pKa = 4.32DD105 pKa = 4.19AGDD108 pKa = 3.7VIKK111 pKa = 10.95LSNTNFQWDD120 pKa = 4.08TQNKK124 pKa = 9.3RR125 pKa = 11.84LTNVADD131 pKa = 3.91PVNNTDD137 pKa = 3.4GVNKK141 pKa = 9.92QFISTNLPNITTVSGISSDD160 pKa = 3.52VTTVAGIASNVTAVASDD177 pKa = 3.27ATDD180 pKa = 2.91IGTVATNIASVNTVATDD197 pKa = 3.25IAKK200 pKa = 10.57VIVVANDD207 pKa = 3.31LNEE210 pKa = 4.25TVSEE214 pKa = 4.29IEE216 pKa = 3.98TAALDD221 pKa = 3.94LQEE224 pKa = 4.36TTSEE228 pKa = 3.89IDD230 pKa = 3.42TVSNNIANVNTVGTNITNVNTVAGVSANVTTVAGINTDD268 pKa = 3.39VTSVAGISSAVSAVNSNSTNINAVNANSANINTVAGIDD306 pKa = 3.58SDD308 pKa = 3.66ITSVANISSDD318 pKa = 3.3VAAVEE323 pKa = 4.42NIAANVTTVAGNNANITTVAGANSNITAVAGAITNVNNVGGAIANVNNVGGSIANVNTVATNLASVNNFAEE394 pKa = 4.46QYY396 pKa = 9.61RR397 pKa = 11.84IASSAPTSSLNVGDD411 pKa = 5.36LYY413 pKa = 11.47FDD415 pKa = 3.51TTANEE420 pKa = 3.92LKK422 pKa = 10.5VYY424 pKa = 10.44KK425 pKa = 10.46SSGWAAAGSTVNGTSQRR442 pKa = 11.84FTYY445 pKa = 9.36TISGTPSSVTGSDD458 pKa = 3.39ANGNTLAYY466 pKa = 9.92DD467 pKa = 3.42AGFADD472 pKa = 4.41VYY474 pKa = 11.19LNGVRR479 pKa = 11.84LSSADD484 pKa = 3.13ITITSGTSVVFASALANGDD503 pKa = 3.63VVDD506 pKa = 4.41VVAYY510 pKa = 7.85GTFNVASINAANISSGTVNNARR532 pKa = 11.84LTGSGAITINGSSVSLGGNITVGEE556 pKa = 4.34TKK558 pKa = 9.09PTIGSISPSTITNAQTSITITGTNFVSVPQVEE590 pKa = 4.8ALNQSTGIWYY600 pKa = 7.87TADD603 pKa = 3.45TISFTNATTLVATFTLSVDD622 pKa = 3.09AQYY625 pKa = 11.81KK626 pKa = 10.17LRR628 pKa = 11.84IEE630 pKa = 4.49NPDD633 pKa = 3.08GNAVLSSSNILTVSDD648 pKa = 3.74APTWSTSAGSLGVFAGNFSGTLATISASSDD678 pKa = 3.18STVAYY683 pKa = 10.72SEE685 pKa = 4.44TTSVLTGAGVTLNTSTGALTTSDD708 pKa = 4.38FGASSTTPTTYY719 pKa = 11.01NFTIRR724 pKa = 11.84ATDD727 pKa = 3.85GEE729 pKa = 4.7GQTTDD734 pKa = 3.29RR735 pKa = 11.84SFSMTSSFGSTGGGQFNN752 pKa = 4.02

Molecular weight: 76.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PIL5|A0A6S4PIL5_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S46-C10 OX=2741074 PE=4 SV=1
MM1 pKa = 7.35YY2 pKa = 10.03MNFILFKK9 pKa = 9.81VTIEE13 pKa = 3.97RR14 pKa = 11.84WSKK17 pKa = 10.33RR18 pKa = 11.84SNLKK22 pKa = 7.48VTKK25 pKa = 10.32RR26 pKa = 11.84NGEE29 pKa = 4.27TIVDD33 pKa = 3.45VAYY36 pKa = 9.78WRR38 pKa = 11.84IYY40 pKa = 9.57LQQ42 pKa = 3.45

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12493

38

832

245.0

27.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.148 ± 0.523

0.985 ± 0.175

5.771 ± 0.199

6.404 ± 0.443

3.882 ± 0.244

6.115 ± 0.296

1.673 ± 0.212

6.604 ± 0.232

8.333 ± 0.693

8.149 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.292

6.388 ± 0.449

2.962 ± 0.208

3.818 ± 0.26

4.09 ± 0.33

7.268 ± 0.459

7.244 ± 0.635

6.107 ± 0.357

1.257 ± 0.136

3.306 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski