Rhizobium etli (strain CFN 42 / ATCC 51251)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; Rhizobium etli

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2K4Q3|Q2K4Q3_RHIEC Hypothetical conserved protein OS=Rhizobium etli (strain CFN 42 / ATCC 51251) OX=347834 GN=RHE_CH03426 PE=3 SV=1
MM1 pKa = 7.67ANHH4 pKa = 6.68KK5 pKa = 9.75PVAADD10 pKa = 3.67VYY12 pKa = 11.13QPIFDD17 pKa = 3.54IDD19 pKa = 3.77KK20 pKa = 10.39PVDD23 pKa = 3.55GNILDD28 pKa = 4.37GSTDD32 pKa = 3.44ADD34 pKa = 3.62GDD36 pKa = 4.08LVRR39 pKa = 11.84LNFVNGQRR47 pKa = 11.84IPQPANPDD55 pKa = 3.27GPATTTTIEE64 pKa = 4.22GKK66 pKa = 10.54YY67 pKa = 9.18GTLTVYY73 pKa = 11.16SNGYY77 pKa = 6.85YY78 pKa = 9.97TYY80 pKa = 10.68EE81 pKa = 4.52LDD83 pKa = 3.24HH84 pKa = 6.82SNPVVSALGPGDD96 pKa = 3.64QLLDD100 pKa = 3.27QFNFKK105 pKa = 10.45ISDD108 pKa = 3.65GKK110 pKa = 10.95GATDD114 pKa = 4.75FGLLNIAVDD123 pKa = 4.0LPEE126 pKa = 6.29RR127 pKa = 11.84GDD129 pKa = 3.2IFVNFEE135 pKa = 4.25DD136 pKa = 3.29VGKK139 pKa = 10.75YY140 pKa = 9.88DD141 pKa = 4.19FPTGYY146 pKa = 10.64KK147 pKa = 10.54GFDD150 pKa = 2.61WGAWHH155 pKa = 7.52DD156 pKa = 4.11GDD158 pKa = 5.13DD159 pKa = 4.37AAIQKK164 pKa = 9.68EE165 pKa = 3.94ADD167 pKa = 3.51GNHH170 pKa = 5.84YY171 pKa = 10.76LSGGVFWTPIQAADD185 pKa = 3.31GGTFQIEE192 pKa = 4.34QFSVANGTSDD202 pKa = 3.6YY203 pKa = 11.55DD204 pKa = 3.55NVLTIEE210 pKa = 4.24GRR212 pKa = 11.84LNGDD216 pKa = 3.39TVFLVTVNVTADD228 pKa = 3.94SIHH231 pKa = 7.19DD232 pKa = 3.78PQAIDD237 pKa = 3.74LSAYY241 pKa = 9.45GQIDD245 pKa = 4.39YY246 pKa = 10.93LVLDD250 pKa = 4.21TEE252 pKa = 4.46PVITEE257 pKa = 3.99TSGPDD262 pKa = 3.43YY263 pKa = 11.38YY264 pKa = 11.19GAQYY268 pKa = 11.44DD269 pKa = 3.41NFYY272 pKa = 11.06FVVV275 pKa = 3.42

Molecular weight: 29.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2KDF8|Q2KDF8_RHIEC Endolytic peptidoglycan transglycosylase RlpA OS=Rhizobium etli (strain CFN 42 / ATCC 51251) OX=347834 GN=rlpA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.17GGRR28 pKa = 11.84KK29 pKa = 9.04VIAARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.21RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5918

3

5921

1851644

32

2825

312.7

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.897 ± 0.038

0.848 ± 0.01

5.646 ± 0.026

5.804 ± 0.029

3.931 ± 0.02

8.244 ± 0.025

2.066 ± 0.016

5.73 ± 0.025

3.682 ± 0.024

9.936 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.013

2.865 ± 0.02

4.892 ± 0.021

3.101 ± 0.019

6.901 ± 0.032

5.887 ± 0.026

5.226 ± 0.022

7.183 ± 0.024

1.296 ± 0.014

2.337 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski