Ruminococcus sp. CAG:353

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2331 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7L0T1|R7L0T1_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:353 OX=1262955 GN=BN622_02179 PE=4 SV=1
MM1 pKa = 7.66LLLCSCTAISEE12 pKa = 4.34NEE14 pKa = 4.13DD15 pKa = 3.74ALPAAATVAEE25 pKa = 4.2PQPYY29 pKa = 9.0PVEE32 pKa = 4.15EE33 pKa = 4.51GNLIFNSSPVTVGSLSPAVTEE54 pKa = 4.41MIYY57 pKa = 10.72EE58 pKa = 4.47LGYY61 pKa = 10.8GDD63 pKa = 5.46RR64 pKa = 11.84LICRR68 pKa = 11.84SSYY71 pKa = 10.88CDD73 pKa = 3.16TPEE76 pKa = 4.1AVLSLPEE83 pKa = 4.24TGSAANPDD91 pKa = 3.01VDD93 pKa = 5.6KK94 pKa = 10.84IISYY98 pKa = 10.66APEE101 pKa = 3.92LLITQSPIANKK112 pKa = 9.09DD113 pKa = 3.5TVRR116 pKa = 11.84LSEE119 pKa = 4.51AGISLLTLPAPTSLEE134 pKa = 3.94EE135 pKa = 5.34LYY137 pKa = 11.2DD138 pKa = 3.82NYY140 pKa = 11.45AALADD145 pKa = 3.84IFAGSIEE152 pKa = 4.25GNSLAEE158 pKa = 3.98NTLADD163 pKa = 3.62MKK165 pKa = 10.99SAVNEE170 pKa = 4.08AKK172 pKa = 10.4SSCEE176 pKa = 3.85SIVFIMNIDD185 pKa = 3.47GDD187 pKa = 4.61EE188 pKa = 4.13ITAGTGDD195 pKa = 4.03SFAGDD200 pKa = 4.05LFSVFGRR207 pKa = 11.84NIAEE211 pKa = 4.11NDD213 pKa = 2.96TDD215 pKa = 3.82YY216 pKa = 11.4TITAEE221 pKa = 4.25EE222 pKa = 5.06LIKK225 pKa = 10.68ADD227 pKa = 3.4PQYY230 pKa = 11.31IFLARR235 pKa = 11.84PLSASDD241 pKa = 3.77FDD243 pKa = 5.01SEE245 pKa = 5.02LAEE248 pKa = 3.95QLSAFSEE255 pKa = 4.23DD256 pKa = 3.42HH257 pKa = 6.33VFSIDD262 pKa = 3.09ASLTEE267 pKa = 4.4RR268 pKa = 11.84PTARR272 pKa = 11.84LAEE275 pKa = 4.57TIRR278 pKa = 11.84SVSEE282 pKa = 3.85VMTDD286 pKa = 3.14TNAEE290 pKa = 4.15TTEE293 pKa = 4.05FTGGYY298 pKa = 10.31AEE300 pKa = 3.81IHH302 pKa = 5.63EE303 pKa = 4.57TSEE306 pKa = 3.84QQ307 pKa = 3.36

Molecular weight: 32.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7KW74|R7KW74_9FIRM Polyribonucleotide nucleotidyltransferase OS=Ruminococcus sp. CAG:353 OX=1262955 GN=pnp PE=3 SV=1
MM1 pKa = 7.82AKK3 pKa = 9.58TSMKK7 pKa = 9.97IKK9 pKa = 9.16QQRR12 pKa = 11.84TPKK15 pKa = 10.22YY16 pKa = 8.2STRR19 pKa = 11.84AYY21 pKa = 10.04NRR23 pKa = 11.84CKK25 pKa = 10.13ICGRR29 pKa = 11.84PHH31 pKa = 7.56AYY33 pKa = 8.38MRR35 pKa = 11.84KK36 pKa = 9.44FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 4.1LAHH49 pKa = 6.87KK50 pKa = 10.13GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2331

0

2331

717514

29

3240

307.8

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.382 ± 0.052

1.814 ± 0.024

6.281 ± 0.043

6.929 ± 0.051

4.215 ± 0.036

7.068 ± 0.049

1.586 ± 0.019

7.562 ± 0.042

6.598 ± 0.043

8.111 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.083 ± 0.025

4.429 ± 0.04

3.412 ± 0.029

2.397 ± 0.023

4.229 ± 0.036

6.682 ± 0.048

5.598 ± 0.041

6.7 ± 0.038

0.789 ± 0.018

4.13 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski