Amino acid dipepetide frequency for Ruminococcus sp. CAG:353

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.454AlaAla: 10.454 ± 0.188	1.293AlaCys: 1.293 ± 0.05	6.238AlaAsp: 6.238 ± 0.107	7.324AlaGlu: 7.324 ± 0.129	3.098AlaPhe: 3.098 ± 0.074	5.828AlaGly: 5.828 ± 0.107	1.197AlaHis: 1.197 ± 0.046	4.589AlaIle: 4.589 ± 0.082	5.051AlaLys: 5.051 ± 0.104	7.026AlaLeu: 7.026 ± 0.104	2.53AlaMet: 2.53 ± 0.064	2.663AlaAsn: 2.663 ± 0.054	2.587AlaPro: 2.587 ± 0.078	2.457AlaGln: 2.457 ± 0.071	2.811AlaArg: 2.811 ± 0.057	4.34AlaSer: 4.34 ± 0.083	2.721AlaThr: 2.721 ± 0.081	8.141AlaVal: 8.141 ± 0.122	0.517AlaTrp: 0.517 ± 0.031	2.948AlaTyr: 2.948 ± 0.068	0.011AlaXaa: 0.011 ± 0.004
Cys	1.555CysAla: 1.555 ± 0.059	0.435CysCys: 0.435 ± 0.026	1.128CysAsp: 1.128 ± 0.037	1.098CysGlu: 1.098 ± 0.038	0.797CysPhe: 0.797 ± 0.033	2.096CysGly: 2.096 ± 0.056	0.347CysHis: 0.347 ± 0.023	1.24CysIle: 1.24 ± 0.044	0.916CysLys: 0.916 ± 0.037	1.14CysLeu: 1.14 ± 0.043	0.428CysMet: 0.428 ± 0.026	0.608CysAsn: 0.608 ± 0.033	0.804CysPro: 0.804 ± 0.042	0.284CysGln: 0.284 ± 0.019	0.949CysArg: 0.949 ± 0.04	1.282CysSer: 1.282 ± 0.046	1.088CysThr: 1.088 ± 0.047	1.164CysVal: 1.164 ± 0.04	0.13CysTrp: 0.13 ± 0.013	0.654CysTyr: 0.654 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	3.962AspAla: 3.962 ± 0.086	1.042AspCys: 1.042 ± 0.042	4.008AspAsp: 4.008 ± 0.091	4.995AspGlu: 4.995 ± 0.103	3.01AspPhe: 3.01 ± 0.073	4.864AspGly: 4.864 ± 0.092	0.833AspHis: 0.833 ± 0.038	6.05AspIle: 6.05 ± 0.095	4.516AspLys: 4.516 ± 0.086	4.012AspLeu: 4.012 ± 0.062	2.309AspMet: 2.309 ± 0.057	2.996AspAsn: 2.996 ± 0.068	2.084AspPro: 2.084 ± 0.053	0.826AspGln: 0.826 ± 0.034	2.64AspArg: 2.64 ± 0.056	3.883AspSer: 3.883 ± 0.095	3.497AspThr: 3.497 ± 0.073	3.808AspVal: 3.808 ± 0.073	0.544AspTrp: 0.544 ± 0.029	2.925AspTyr: 2.925 ± 0.074	0.0AspXaa: 0.0 ± 0.0
Glu	5.469GluAla: 5.469 ± 0.102	0.932GluCys: 0.932 ± 0.036	3.67GluAsp: 3.67 ± 0.076	5.242GluGlu: 5.242 ± 0.099	2.523GluPhe: 2.523 ± 0.062	3.894GluGly: 3.894 ± 0.079	1.279GluHis: 1.279 ± 0.039	5.25GluIle: 5.25 ± 0.087	6.291GluLys: 6.291 ± 0.11	6.461GluLeu: 6.461 ± 0.112	2.319GluMet: 2.319 ± 0.062	4.393GluAsn: 4.393 ± 0.081	1.855GluPro: 1.855 ± 0.05	2.24GluGln: 2.24 ± 0.066	3.073GluArg: 3.073 ± 0.061	3.371GluSer: 3.371 ± 0.061	3.805GluThr: 3.805 ± 0.086	3.483GluVal: 3.483 ± 0.077	0.51GluTrp: 0.51 ± 0.026	3.231GluTyr: 3.231 ± 0.073	0.0GluXaa: 0.0 ± 0.0
Phe	3.44PheAla: 3.44 ± 0.077	0.842PheCys: 0.842 ± 0.033	2.998PheAsp: 2.998 ± 0.066	2.818PheGlu: 2.818 ± 0.069	1.881PhePhe: 1.881 ± 0.06	3.388PheGly: 3.388 ± 0.072	0.59PheHis: 0.59 ± 0.031	3.238PheIle: 3.238 ± 0.079	2.142PheLys: 2.142 ± 0.053	3.065PheLeu: 3.065 ± 0.068	1.215PheMet: 1.215 ± 0.041	1.674PheAsn: 1.674 ± 0.048	1.37PhePro: 1.37 ± 0.045	0.715PheGln: 0.715 ± 0.029	1.919PheArg: 1.919 ± 0.054	3.41PheSer: 3.41 ± 0.077	2.663PheThr: 2.663 ± 0.071	2.723PheVal: 2.723 ± 0.067	0.332PheTrp: 0.332 ± 0.025	1.724PheTyr: 1.724 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	5.364GlyAla: 5.364 ± 0.096	1.572GlyCys: 1.572 ± 0.051	4.043GlyAsp: 4.043 ± 0.083	4.545GlyGlu: 4.545 ± 0.095	3.3GlyPhe: 3.3 ± 0.074	5.29GlyGly: 5.29 ± 0.112	1.196GlyHis: 1.196 ± 0.042	6.029GlyIle: 6.029 ± 0.102	5.197GlyLys: 5.197 ± 0.083	5.423GlyLeu: 5.423 ± 0.09	2.364GlyMet: 2.364 ± 0.061	2.992GlyAsn: 2.992 ± 0.076	1.008GlyPro: 1.008 ± 0.037	1.599GlyGln: 1.599 ± 0.049	3.165GlyArg: 3.165 ± 0.079	4.55GlySer: 4.55 ± 0.08	4.261GlyThr: 4.261 ± 0.076	4.846GlyVal: 4.846 ± 0.076	0.637GlyTrp: 0.637 ± 0.035	3.302GlyTyr: 3.302 ± 0.07	0.001GlyXaa: 0.001 ± 0.001
His	0.964HisAla: 0.964 ± 0.032	0.348HisCys: 0.348 ± 0.023	0.992HisAsp: 0.992 ± 0.038	0.952HisGlu: 0.952 ± 0.037	0.824HisPhe: 0.824 ± 0.034	1.275HisGly: 1.275 ± 0.044	0.309HisHis: 0.309 ± 0.024	1.318HisIle: 1.318 ± 0.044	0.935HisLys: 0.935 ± 0.035	1.213HisLeu: 1.213 ± 0.041	0.443HisMet: 0.443 ± 0.024	0.722HisAsn: 0.722 ± 0.03	0.709HisPro: 0.709 ± 0.033	0.392HisGln: 0.392 ± 0.022	0.728HisArg: 0.728 ± 0.034	1.072HisSer: 1.072 ± 0.043	1.01HisThr: 1.01 ± 0.041	0.8HisVal: 0.8 ± 0.034	0.146HisTrp: 0.146 ± 0.014	0.709HisTyr: 0.709 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.167IleAla: 6.167 ± 0.101	1.744IleCys: 1.744 ± 0.052	4.596IleAsp: 4.596 ± 0.079	4.365IleGlu: 4.365 ± 0.083	3.21IlePhe: 3.21 ± 0.074	4.93IleGly: 4.93 ± 0.089	1.104IleHis: 1.104 ± 0.04	6.286IleIle: 6.286 ± 0.115	4.556IleLys: 4.556 ± 0.082	5.626IleLeu: 5.626 ± 0.11	2.206IleMet: 2.206 ± 0.056	3.345IleAsn: 3.345 ± 0.074	3.298IlePro: 3.298 ± 0.061	1.357IleGln: 1.357 ± 0.044	3.271IleArg: 3.271 ± 0.069	6.333IleSer: 6.333 ± 0.103	4.992IleThr: 4.992 ± 0.081	4.776IleVal: 4.776 ± 0.083	0.532IleTrp: 0.532 ± 0.026	2.924IleTyr: 2.924 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	5.674LysAla: 5.674 ± 0.09	1.02LysCys: 1.02 ± 0.041	3.49LysAsp: 3.49 ± 0.057	4.541LysGlu: 4.541 ± 0.08	2.287LysPhe: 2.287 ± 0.056	4.226LysGly: 4.226 ± 0.076	0.946LysHis: 0.946 ± 0.039	4.879LysIle: 4.879 ± 0.097	5.364LysLys: 5.364 ± 0.086	5.989LysLeu: 5.989 ± 0.083	2.259LysMet: 2.259 ± 0.053	3.475LysAsn: 3.475 ± 0.079	2.223LysPro: 2.223 ± 0.062	1.824LysGln: 1.824 ± 0.057	2.938LysArg: 2.938 ± 0.067	3.965LysSer: 3.965 ± 0.078	3.541LysThr: 3.541 ± 0.073	3.706LysVal: 3.706 ± 0.082	0.576LysTrp: 0.576 ± 0.031	3.052LysTyr: 3.052 ± 0.067	0.0LysXaa: 0.0 ± 0.0
Leu	6.733LeuAla: 6.733 ± 0.112	1.757LeuCys: 1.757 ± 0.054	4.932LeuAsp: 4.932 ± 0.093	4.891LeuGlu: 4.891 ± 0.088	3.434LeuPhe: 3.434 ± 0.081	5.357LeuGly: 5.357 ± 0.09	1.261LeuHis: 1.261 ± 0.041	6.28LeuIle: 6.28 ± 0.114	5.303LeuLys: 5.303 ± 0.085	6.773LeuLeu: 6.773 ± 0.139	2.559LeuMet: 2.559 ± 0.062	3.526LeuAsn: 3.526 ± 0.075	3.288LeuPro: 3.288 ± 0.073	1.567LeuGln: 1.567 ± 0.05	3.631LeuArg: 3.631 ± 0.077	6.428LeuSer: 6.428 ± 0.112	5.284LeuThr: 5.284 ± 0.085	4.407LeuVal: 4.407 ± 0.081	0.638LeuTrp: 0.638 ± 0.034	3.063LeuTyr: 3.063 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	2.454MetAla: 2.454 ± 0.063	0.492MetCys: 0.492 ± 0.03	1.639MetAsp: 1.639 ± 0.044	1.799MetGlu: 1.799 ± 0.051	1.167MetPhe: 1.167 ± 0.044	2.36MetGly: 2.36 ± 0.067	0.438MetHis: 0.438 ± 0.027	2.102MetIle: 2.102 ± 0.062	2.392MetLys: 2.392 ± 0.056	2.927MetLeu: 2.927 ± 0.074	0.905MetMet: 0.905 ± 0.038	1.691MetAsn: 1.691 ± 0.049	1.3MetPro: 1.3 ± 0.047	0.884MetGln: 0.884 ± 0.031	1.325MetArg: 1.325 ± 0.046	2.169MetSer: 2.169 ± 0.059	1.894MetThr: 1.894 ± 0.055	1.647MetVal: 1.647 ± 0.052	0.243MetTrp: 0.243 ± 0.021	1.009MetTyr: 1.009 ± 0.041	0.0MetXaa: 0.0 ± 0.0
Asn	3.649AsnAla: 3.649 ± 0.065	0.715AsnCys: 0.715 ± 0.034	2.772AsnAsp: 2.772 ± 0.077	2.97AsnGlu: 2.97 ± 0.066	1.717AsnPhe: 1.717 ± 0.055	4.003AsnGly: 4.003 ± 0.1	0.585AsnHis: 0.585 ± 0.033	3.98AsnIle: 3.98 ± 0.077	2.583AsnLys: 2.583 ± 0.06	2.923AsnLeu: 2.923 ± 0.06	1.356AsnMet: 1.356 ± 0.041	1.955AsnAsn: 1.955 ± 0.066	1.792AsnPro: 1.792 ± 0.052	0.886AsnGln: 0.886 ± 0.04	1.792AsnArg: 1.792 ± 0.051	2.899AsnSer: 2.899 ± 0.08	2.484AsnThr: 2.484 ± 0.06	3.079AsnVal: 3.079 ± 0.068	0.329AsnTrp: 0.329 ± 0.021	1.824AsnTyr: 1.824 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	3.14ProAla: 3.14 ± 0.079	0.56ProCys: 0.56 ± 0.029	2.87ProAsp: 2.87 ± 0.063	3.462ProGlu: 3.462 ± 0.078	1.525ProPhe: 1.525 ± 0.045	1.406ProGly: 1.406 ± 0.05	0.612ProHis: 0.612 ± 0.033	1.922ProIle: 1.922 ± 0.049	2.026ProLys: 2.026 ± 0.058	2.737ProLeu: 2.737 ± 0.061	1.026ProMet: 1.026 ± 0.036	1.292ProAsn: 1.292 ± 0.047	0.9ProPro: 0.9 ± 0.035	1.146ProGln: 1.146 ± 0.037	1.076ProArg: 1.076 ± 0.039	2.067ProSer: 2.067 ± 0.06	1.582ProThr: 1.582 ± 0.047	3.076ProVal: 3.076 ± 0.07	0.273ProTrp: 0.273 ± 0.023	1.417ProTyr: 1.417 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	1.999GlnAla: 1.999 ± 0.06	0.334GlnCys: 0.334 ± 0.022	1.019GlnAsp: 1.019 ± 0.036	1.21GlnGlu: 1.21 ± 0.043	0.877GlnPhe: 0.877 ± 0.035	1.468GlnGly: 1.468 ± 0.043	0.385GlnHis: 0.385 ± 0.024	1.883GlnIle: 1.883 ± 0.053	1.82GlnLys: 1.82 ± 0.061	2.327GlnLeu: 2.327 ± 0.052	0.949GlnMet: 0.949 ± 0.039	1.33GlnAsn: 1.33 ± 0.054	0.769GlnPro: 0.769 ± 0.037	0.859GlnGln: 0.859 ± 0.044	1.147GlnArg: 1.147 ± 0.042	1.564GlnSer: 1.564 ± 0.048	1.384GlnThr: 1.384 ± 0.043	1.391GlnVal: 1.391 ± 0.047	0.245GlnTrp: 0.245 ± 0.022	1.008GlnTyr: 1.008 ± 0.043	0.001GlnXaa: 0.001 ± 0.001
Arg	2.786ArgAla: 2.786 ± 0.065	0.757ArgCys: 0.757 ± 0.037	2.261ArgAsp: 2.261 ± 0.057	3.083ArgGlu: 3.083 ± 0.078	1.908ArgPhe: 1.908 ± 0.053	2.226ArgGly: 2.226 ± 0.067	0.79ArgHis: 0.79 ± 0.036	3.518ArgIle: 3.518 ± 0.071	3.1ArgLys: 3.1 ± 0.065	4.121ArgLeu: 4.121 ± 0.091	1.316ArgMet: 1.316 ± 0.048	1.829ArgAsn: 1.829 ± 0.048	1.25ArgPro: 1.25 ± 0.04	1.387ArgGln: 1.387 ± 0.044	2.275ArgArg: 2.275 ± 0.062	2.794ArgSer: 2.794 ± 0.068	2.371ArgThr: 2.371 ± 0.059	2.365ArgVal: 2.365 ± 0.053	0.343ArgTrp: 0.343 ± 0.022	1.808ArgTyr: 1.808 ± 0.053	0.001ArgXaa: 0.001 ± 0.001
Ser	6.199SerAla: 6.199 ± 0.102	1.154SerCys: 1.154 ± 0.038	4.95SerAsp: 4.95 ± 0.098	5.173SerGlu: 5.173 ± 0.106	3.019SerPhe: 3.019 ± 0.063	5.766SerGly: 5.766 ± 0.109	1.073SerHis: 1.073 ± 0.034	3.941SerIle: 3.941 ± 0.075	3.621SerLys: 3.621 ± 0.073	5.281SerLeu: 5.281 ± 0.088	1.803SerMet: 1.803 ± 0.043	2.273SerAsn: 2.273 ± 0.067	2.293SerPro: 2.293 ± 0.055	1.677SerGln: 1.677 ± 0.051	2.803SerArg: 2.803 ± 0.063	4.464SerSer: 4.464 ± 0.102	3.107SerThr: 3.107 ± 0.072	5.105SerVal: 5.105 ± 0.076	0.52SerTrp: 0.52 ± 0.028	2.598SerTyr: 2.598 ± 0.068	0.001SerXaa: 0.001 ± 0.002
Thr	6.063ThrAla: 6.063 ± 0.111	0.769ThrCys: 0.769 ± 0.031	4.156ThrAsp: 4.156 ± 0.075	4.228ThrGlu: 4.228 ± 0.085	2.188ThrPhe: 2.188 ± 0.065	4.578ThrGly: 4.578 ± 0.101	0.886ThrHis: 0.886 ± 0.033	3.547ThrIle: 3.547 ± 0.075	2.707ThrLys: 2.707 ± 0.06	4.332ThrLeu: 4.332 ± 0.08	1.331ThrMet: 1.331 ± 0.044	1.893ThrAsn: 1.893 ± 0.063	2.301ThrPro: 2.301 ± 0.059	1.189ThrGln: 1.189 ± 0.042	1.774ThrArg: 1.774 ± 0.048	3.316ThrSer: 3.316 ± 0.078	2.786ThrThr: 2.786 ± 0.1	5.472ThrVal: 5.472 ± 0.096	0.393ThrTrp: 0.393 ± 0.026	2.071ThrTyr: 2.071 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.164ValAla: 4.164 ± 0.096	1.483ValCys: 1.483 ± 0.05	3.468ValAsp: 3.468 ± 0.074	3.527ValGlu: 3.527 ± 0.086	3.154ValPhe: 3.154 ± 0.058	4.031ValGly: 4.031 ± 0.072	1.048ValHis: 1.048 ± 0.039	5.87ValIle: 5.87 ± 0.089	4.633ValLys: 4.633 ± 0.097	5.946ValLeu: 5.946 ± 0.094	2.231ValMet: 2.231 ± 0.061	3.077ValAsn: 3.077 ± 0.07	2.644ValPro: 2.644 ± 0.053	1.526ValGln: 1.526 ± 0.046	2.899ValArg: 2.899 ± 0.066	5.303ValSer: 5.303 ± 0.097	4.687ValThr: 4.687 ± 0.1	4.026ValVal: 4.026 ± 0.087	0.576ValTrp: 0.576 ± 0.026	2.707ValTyr: 2.707 ± 0.071	0.001ValXaa: 0.001 ± 0.001
Trp	0.52TrpAla: 0.52 ± 0.029	0.19TrpCys: 0.19 ± 0.016	0.52TrpAsp: 0.52 ± 0.03	0.488TrpGlu: 0.488 ± 0.027	0.36TrpPhe: 0.36 ± 0.025	0.63TrpGly: 0.63 ± 0.032	0.209TrpHis: 0.209 ± 0.018	0.507TrpIle: 0.507 ± 0.031	0.475TrpLys: 0.475 ± 0.026	0.786TrpLeu: 0.786 ± 0.032	0.192TrpMet: 0.192 ± 0.016	0.499TrpAsn: 0.499 ± 0.029	0.127TrpPro: 0.127 ± 0.016	0.322TrpGln: 0.322 ± 0.018	0.297TrpArg: 0.297 ± 0.021	0.514TrpSer: 0.514 ± 0.03	0.4TrpThr: 0.4 ± 0.029	0.407TrpVal: 0.407 ± 0.024	0.1TrpTrp: 0.1 ± 0.012	0.347TrpTyr: 0.347 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.066TyrAla: 3.066 ± 0.079	0.702TyrCys: 0.702 ± 0.031	3.056TyrAsp: 3.056 ± 0.072	2.58TyrGlu: 2.58 ± 0.063	1.868TyrPhe: 1.868 ± 0.056	3.069TyrGly: 3.069 ± 0.071	0.774TyrHis: 0.774 ± 0.035	3.186TyrIle: 3.186 ± 0.066	2.348TyrLys: 2.348 ± 0.068	3.002TyrLeu: 3.002 ± 0.068	1.091TyrMet: 1.091 ± 0.039	2.063TyrAsn: 2.063 ± 0.054	1.512TyrPro: 1.512 ± 0.049	0.831TyrGln: 0.831 ± 0.036	1.77TyrArg: 1.77 ± 0.046	3.097TyrSer: 3.097 ± 0.073	2.422TyrThr: 2.422 ± 0.071	2.58TyrVal: 2.58 ± 0.065	0.307TyrTrp: 0.307 ± 0.019	1.979TyrTyr: 1.979 ± 0.074	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.004XaaArg: 0.004 ± 0.003	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.003XaaTyr: 0.003 ± 0.002	0.028XaaXaa: 0.028 ± 0.01

Statistics based on 2331 proteins (717514 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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