Amino acid dipepetide frequency for Bacillus weihaiensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.601AlaAla: 4.601 ± 0.087	0.55AlaCys: 0.55 ± 0.026	2.982AlaAsp: 2.982 ± 0.064	4.196AlaGlu: 4.196 ± 0.075	3.033AlaPhe: 3.033 ± 0.055	4.431AlaGly: 4.431 ± 0.07	1.22AlaHis: 1.22 ± 0.034	5.705AlaIle: 5.705 ± 0.089	4.583AlaLys: 4.583 ± 0.057	6.594AlaLeu: 6.594 ± 0.088	1.775AlaMet: 1.775 ± 0.038	2.738AlaAsn: 2.738 ± 0.047	1.793AlaPro: 1.793 ± 0.043	1.922AlaGln: 1.922 ± 0.041	2.263AlaArg: 2.263 ± 0.047	3.991AlaSer: 3.991 ± 0.065	3.556AlaThr: 3.556 ± 0.056	4.643AlaVal: 4.643 ± 0.072	0.536AlaTrp: 0.536 ± 0.023	2.195AlaTyr: 2.195 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.395CysAla: 0.395 ± 0.02	0.101CysCys: 0.101 ± 0.012	0.372CysAsp: 0.372 ± 0.019	0.468CysGlu: 0.468 ± 0.022	0.333CysPhe: 0.333 ± 0.017	0.592CysGly: 0.592 ± 0.026	0.207CysHis: 0.207 ± 0.016	0.549CysIle: 0.549 ± 0.02	0.386CysLys: 0.386 ± 0.02	0.659CysLeu: 0.659 ± 0.028	0.205CysMet: 0.205 ± 0.014	0.297CysAsn: 0.297 ± 0.017	0.324CysPro: 0.324 ± 0.016	0.214CysGln: 0.214 ± 0.015	0.253CysArg: 0.253 ± 0.016	0.509CysSer: 0.509 ± 0.023	0.368CysThr: 0.368 ± 0.017	0.42CysVal: 0.42 ± 0.021	0.072CysTrp: 0.072 ± 0.009	0.256CysTyr: 0.256 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	2.865AspAla: 2.865 ± 0.054	0.378AspCys: 0.378 ± 0.021	2.472AspAsp: 2.472 ± 0.06	4.563AspGlu: 4.563 ± 0.065	2.335AspPhe: 2.335 ± 0.046	3.144AspGly: 3.144 ± 0.066	1.259AspHis: 1.259 ± 0.032	4.027AspIle: 4.027 ± 0.065	3.072AspLys: 3.072 ± 0.05	5.134AspLeu: 5.134 ± 0.079	1.345AspMet: 1.345 ± 0.034	1.757AspAsn: 1.757 ± 0.043	1.861AspPro: 1.861 ± 0.037	2.318AspGln: 2.318 ± 0.051	2.151AspArg: 2.151 ± 0.047	2.794AspSer: 2.794 ± 0.048	2.502AspThr: 2.502 ± 0.058	3.943AspVal: 3.943 ± 0.062	0.568AspTrp: 0.568 ± 0.023	2.181AspTyr: 2.181 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.243GluAla: 5.243 ± 0.071	0.427GluCys: 0.427 ± 0.023	4.064GluAsp: 4.064 ± 0.062	8.042GluGlu: 8.042 ± 0.123	2.767GluPhe: 2.767 ± 0.053	4.443GluGly: 4.443 ± 0.075	1.593GluHis: 1.593 ± 0.036	5.923GluIle: 5.923 ± 0.077	7.081GluLys: 7.081 ± 0.105	7.719GluLeu: 7.719 ± 0.08	2.405GluMet: 2.405 ± 0.044	3.969GluAsn: 3.969 ± 0.063	1.913GluPro: 1.913 ± 0.035	3.432GluGln: 3.432 ± 0.065	3.351GluArg: 3.351 ± 0.058	3.64GluSer: 3.64 ± 0.063	3.987GluThr: 3.987 ± 0.061	5.465GluVal: 5.465 ± 0.082	0.813GluTrp: 0.813 ± 0.029	2.504GluTyr: 2.504 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	2.712PheAla: 2.712 ± 0.051	0.347PheCys: 0.347 ± 0.019	2.312PheAsp: 2.312 ± 0.053	2.879PheGlu: 2.879 ± 0.049	2.574PhePhe: 2.574 ± 0.056	3.031PheGly: 3.031 ± 0.062	1.153PheHis: 1.153 ± 0.035	4.298PheIle: 4.298 ± 0.074	2.535PheLys: 2.535 ± 0.048	4.948PheLeu: 4.948 ± 0.076	1.122PheMet: 1.122 ± 0.035	1.846PheAsn: 1.846 ± 0.041	1.586PhePro: 1.586 ± 0.041	1.771PheGln: 1.771 ± 0.04	1.416PheArg: 1.416 ± 0.038	3.447PheSer: 3.447 ± 0.063	2.848PheThr: 2.848 ± 0.054	3.129PheVal: 3.129 ± 0.057	0.433PheTrp: 0.433 ± 0.021	1.771PheTyr: 1.771 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.41GlyAla: 4.41 ± 0.073	0.571GlyCys: 0.571 ± 0.023	3.108GlyAsp: 3.108 ± 0.052	4.629GlyGlu: 4.629 ± 0.076	3.297GlyPhe: 3.297 ± 0.058	4.411GlyGly: 4.411 ± 0.079	1.325GlyHis: 1.325 ± 0.032	5.717GlyIle: 5.717 ± 0.09	4.802GlyLys: 4.802 ± 0.07	6.263GlyLeu: 6.263 ± 0.072	1.982GlyMet: 1.982 ± 0.041	2.555GlyAsn: 2.555 ± 0.059	1.565GlyPro: 1.565 ± 0.037	2.056GlyGln: 2.056 ± 0.046	2.365GlyArg: 2.365 ± 0.048	3.807GlySer: 3.807 ± 0.067	3.808GlyThr: 3.808 ± 0.057	4.824GlyVal: 4.824 ± 0.076	0.688GlyTrp: 0.688 ± 0.027	2.747GlyTyr: 2.747 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.315HisAla: 1.315 ± 0.038	0.205HisCys: 0.205 ± 0.013	1.099HisAsp: 1.099 ± 0.037	1.565HisGlu: 1.565 ± 0.041	1.136HisPhe: 1.136 ± 0.033	1.267HisGly: 1.267 ± 0.035	0.761HisHis: 0.761 ± 0.026	1.608HisIle: 1.608 ± 0.042	1.154HisLys: 1.154 ± 0.036	2.211HisLeu: 2.211 ± 0.052	0.517HisMet: 0.517 ± 0.023	0.818HisAsn: 0.818 ± 0.026	1.114HisPro: 1.114 ± 0.027	0.91HisGln: 0.91 ± 0.027	0.781HisArg: 0.781 ± 0.028	1.407HisSer: 1.407 ± 0.037	1.188HisThr: 1.188 ± 0.033	1.548HisVal: 1.548 ± 0.04	0.194HisTrp: 0.194 ± 0.013	1.023HisTyr: 1.023 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.54IleAla: 5.54 ± 0.091	0.655IleCys: 0.655 ± 0.024	4.496IleAsp: 4.496 ± 0.068	6.213IleGlu: 6.213 ± 0.085	3.452IlePhe: 3.452 ± 0.066	6.003IleGly: 6.003 ± 0.089	1.89IleHis: 1.89 ± 0.043	6.187IleIle: 6.187 ± 0.102	4.761IleLys: 4.761 ± 0.074	7.463IleLeu: 7.463 ± 0.096	1.839IleMet: 1.839 ± 0.048	3.286IleAsn: 3.286 ± 0.062	3.384IlePro: 3.384 ± 0.056	3.13IleGln: 3.13 ± 0.057	2.996IleArg: 2.996 ± 0.056	5.465IleSer: 5.465 ± 0.071	4.564IleThr: 4.564 ± 0.066	5.712IleVal: 5.712 ± 0.085	0.638IleTrp: 0.638 ± 0.029	2.588IleTyr: 2.588 ± 0.056	0.0IleXaa: 0.0 ± 0.0
Lys	4.46LysAla: 4.46 ± 0.069	0.338LysCys: 0.338 ± 0.016	3.973LysAsp: 3.973 ± 0.054	7.566LysGlu: 7.566 ± 0.095	1.883LysPhe: 1.883 ± 0.037	4.501LysGly: 4.501 ± 0.064	1.387LysHis: 1.387 ± 0.034	4.926LysIle: 4.926 ± 0.08	6.321LysLys: 6.321 ± 0.097	6.204LysLeu: 6.204 ± 0.079	2.186LysMet: 2.186 ± 0.046	3.563LysAsn: 3.563 ± 0.059	2.19LysPro: 2.19 ± 0.048	3.435LysGln: 3.435 ± 0.063	3.237LysArg: 3.237 ± 0.055	3.687LysSer: 3.687 ± 0.061	3.686LysThr: 3.686 ± 0.066	4.945LysVal: 4.945 ± 0.066	0.809LysTrp: 0.809 ± 0.03	2.228LysTyr: 2.228 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.787LeuAla: 6.787 ± 0.094	0.661LeuCys: 0.661 ± 0.028	4.789LeuAsp: 4.789 ± 0.075	6.828LeuGlu: 6.828 ± 0.093	5.226LeuPhe: 5.226 ± 0.091	6.219LeuGly: 6.219 ± 0.085	2.218LeuHis: 2.218 ± 0.053	7.245LeuIle: 7.245 ± 0.089	6.838LeuLys: 6.838 ± 0.089	10.774LeuLeu: 10.774 ± 0.127	2.427LeuMet: 2.427 ± 0.05	4.264LeuAsn: 4.264 ± 0.069	3.812LeuPro: 3.812 ± 0.056	3.659LeuGln: 3.659 ± 0.064	3.354LeuArg: 3.354 ± 0.062	7.397LeuSer: 7.397 ± 0.084	6.109LeuThr: 6.109 ± 0.07	6.592LeuVal: 6.592 ± 0.081	0.8LeuTrp: 0.8 ± 0.026	3.266LeuTyr: 3.266 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	1.694MetAla: 1.694 ± 0.044	0.136MetCys: 0.136 ± 0.011	1.335MetAsp: 1.335 ± 0.031	1.893MetGlu: 1.893 ± 0.042	1.099MetPhe: 1.099 ± 0.036	1.649MetGly: 1.649 ± 0.04	0.379MetHis: 0.379 ± 0.02	2.323MetIle: 2.323 ± 0.051	2.711MetLys: 2.711 ± 0.046	2.426MetLeu: 2.426 ± 0.051	0.905MetMet: 0.905 ± 0.033	1.714MetAsn: 1.714 ± 0.042	0.861MetPro: 0.861 ± 0.029	0.788MetGln: 0.788 ± 0.025	0.97MetArg: 0.97 ± 0.03	1.731MetSer: 1.731 ± 0.037	1.64MetThr: 1.64 ± 0.04	1.787MetVal: 1.787 ± 0.042	0.19MetTrp: 0.19 ± 0.012	0.786MetTyr: 0.786 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.436AsnAla: 2.436 ± 0.048	0.264AsnCys: 0.264 ± 0.014	2.3AsnAsp: 2.3 ± 0.052	4.164AsnGlu: 4.164 ± 0.058	1.583AsnPhe: 1.583 ± 0.039	3.287AsnGly: 3.287 ± 0.055	1.14AsnHis: 1.14 ± 0.031	3.21AsnIle: 3.21 ± 0.056	3.191AsnLys: 3.191 ± 0.059	3.791AsnLeu: 3.791 ± 0.056	1.194AsnMet: 1.194 ± 0.029	1.918AsnAsn: 1.918 ± 0.046	2.009AsnPro: 2.009 ± 0.045	2.157AsnGln: 2.157 ± 0.05	1.965AsnArg: 1.965 ± 0.041	2.487AsnSer: 2.487 ± 0.054	2.227AsnThr: 2.227 ± 0.052	3.073AsnVal: 3.073 ± 0.053	0.488AsnTrp: 0.488 ± 0.023	1.496AsnTyr: 1.496 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	1.938ProAla: 1.938 ± 0.045	0.196ProCys: 0.196 ± 0.013	1.804ProAsp: 1.804 ± 0.039	2.652ProGlu: 2.652 ± 0.054	1.953ProPhe: 1.953 ± 0.042	2.054ProGly: 2.054 ± 0.053	0.775ProHis: 0.775 ± 0.03	3.0ProIle: 3.0 ± 0.061	2.138ProLys: 2.138 ± 0.042	3.408ProLeu: 3.408 ± 0.059	0.737ProMet: 0.737 ± 0.03	1.669ProAsn: 1.669 ± 0.039	0.965ProPro: 0.965 ± 0.027	1.014ProGln: 1.014 ± 0.033	0.988ProArg: 0.988 ± 0.027	2.291ProSer: 2.291 ± 0.046	2.222ProThr: 2.222 ± 0.043	2.466ProVal: 2.466 ± 0.045	0.342ProTrp: 0.342 ± 0.017	1.441ProTyr: 1.441 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.612GlnAla: 2.612 ± 0.047	0.193GlnCys: 0.193 ± 0.013	1.796GlnAsp: 1.796 ± 0.049	3.233GlnGlu: 3.233 ± 0.063	1.746GlnPhe: 1.746 ± 0.041	2.024GlnGly: 2.024 ± 0.042	0.851GlnHis: 0.851 ± 0.031	2.657GlnIle: 2.657 ± 0.051	2.825GlnLys: 2.825 ± 0.056	4.236GlnLeu: 4.236 ± 0.072	1.023GlnMet: 1.023 ± 0.031	1.603GlnAsn: 1.603 ± 0.044	1.166GlnPro: 1.166 ± 0.036	1.921GlnGln: 1.921 ± 0.055	1.445GlnArg: 1.445 ± 0.035	2.37GlnSer: 2.37 ± 0.046	2.097GlnThr: 2.097 ± 0.041	2.517GlnVal: 2.517 ± 0.049	0.386GlnTrp: 0.386 ± 0.015	1.368GlnTyr: 1.368 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.207ArgAla: 2.207 ± 0.052	0.224ArgCys: 0.224 ± 0.015	1.946ArgAsp: 1.946 ± 0.048	3.162ArgGlu: 3.162 ± 0.054	1.753ArgPhe: 1.753 ± 0.037	2.195ArgGly: 2.195 ± 0.051	0.745ArgHis: 0.745 ± 0.023	2.916ArgIle: 2.916 ± 0.052	3.157ArgLys: 3.157 ± 0.056	3.569ArgLeu: 3.569 ± 0.058	1.203ArgMet: 1.203 ± 0.031	1.831ArgAsn: 1.831 ± 0.045	1.165ArgPro: 1.165 ± 0.029	1.373ArgGln: 1.373 ± 0.035	1.66ArgArg: 1.66 ± 0.049	2.153ArgSer: 2.153 ± 0.046	2.007ArgThr: 2.007 ± 0.041	2.512ArgVal: 2.512 ± 0.047	0.362ArgTrp: 0.362 ± 0.019	1.424ArgTyr: 1.424 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.505SerAla: 3.505 ± 0.056	0.408SerCys: 0.408 ± 0.02	2.896SerAsp: 2.896 ± 0.047	4.336SerGlu: 4.336 ± 0.063	3.626SerPhe: 3.626 ± 0.067	4.144SerGly: 4.144 ± 0.072	1.336SerHis: 1.336 ± 0.036	5.672SerIle: 5.672 ± 0.09	4.226SerLys: 4.226 ± 0.07	6.783SerLeu: 6.783 ± 0.085	1.801SerMet: 1.801 ± 0.043	2.662SerAsn: 2.662 ± 0.054	2.052SerPro: 2.052 ± 0.043	2.123SerGln: 2.123 ± 0.052	2.208SerArg: 2.208 ± 0.04	4.465SerSer: 4.465 ± 0.082	3.598SerThr: 3.598 ± 0.062	4.222SerVal: 4.222 ± 0.06	0.598SerTrp: 0.598 ± 0.025	2.45SerTyr: 2.45 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.465ThrAla: 3.465 ± 0.059	0.393ThrCys: 0.393 ± 0.02	2.724ThrAsp: 2.724 ± 0.055	3.701ThrGlu: 3.701 ± 0.07	2.868ThrPhe: 2.868 ± 0.065	3.876ThrGly: 3.876 ± 0.057	1.142ThrHis: 1.142 ± 0.03	5.191ThrIle: 5.191 ± 0.061	3.746ThrLys: 3.746 ± 0.057	5.64ThrLeu: 5.64 ± 0.071	1.348ThrMet: 1.348 ± 0.034	2.743ThrAsn: 2.743 ± 0.048	2.215ThrPro: 2.215 ± 0.046	1.566ThrGln: 1.566 ± 0.037	1.82ThrArg: 1.82 ± 0.038	3.778ThrSer: 3.778 ± 0.071	3.308ThrThr: 3.308 ± 0.068	4.075ThrVal: 4.075 ± 0.057	0.504ThrTrp: 0.504 ± 0.023	2.332ThrTyr: 2.332 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.684ValAla: 4.684 ± 0.068	0.546ValCys: 0.546 ± 0.02	3.743ValAsp: 3.743 ± 0.059	5.305ValGlu: 5.305 ± 0.075	3.074ValPhe: 3.074 ± 0.05	4.65ValGly: 4.65 ± 0.071	1.322ValHis: 1.322 ± 0.032	5.758ValIle: 5.758 ± 0.075	5.123ValLys: 5.123 ± 0.078	6.607ValLeu: 6.607 ± 0.087	1.804ValMet: 1.804 ± 0.041	3.208ValAsn: 3.208 ± 0.053	2.474ValPro: 2.474 ± 0.054	2.344ValGln: 2.344 ± 0.046	2.469ValArg: 2.469 ± 0.051	4.717ValSer: 4.717 ± 0.064	4.266ValThr: 4.266 ± 0.061	5.134ValVal: 5.134 ± 0.079	0.586ValTrp: 0.586 ± 0.025	2.242ValTyr: 2.242 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.53TrpAla: 0.53 ± 0.021	0.081TrpCys: 0.081 ± 0.008	0.501TrpAsp: 0.501 ± 0.023	0.639TrpGlu: 0.639 ± 0.027	0.511TrpPhe: 0.511 ± 0.023	0.602TrpGly: 0.602 ± 0.024	0.189TrpHis: 0.189 ± 0.014	0.799TrpIle: 0.799 ± 0.029	0.678TrpLys: 0.678 ± 0.027	1.019TrpLeu: 1.019 ± 0.034	0.335TrpMet: 0.335 ± 0.017	0.522TrpAsn: 0.522 ± 0.019	0.223TrpPro: 0.223 ± 0.013	0.326TrpGln: 0.326 ± 0.018	0.362TrpArg: 0.362 ± 0.02	0.597TrpSer: 0.597 ± 0.023	0.492TrpThr: 0.492 ± 0.022	0.607TrpVal: 0.607 ± 0.026	0.132TrpTrp: 0.132 ± 0.012	0.35TrpTyr: 0.35 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.909TyrAla: 1.909 ± 0.043	0.308TyrCys: 0.308 ± 0.019	1.955TyrAsp: 1.955 ± 0.037	2.749TyrGlu: 2.749 ± 0.051	1.91TyrPhe: 1.91 ± 0.041	2.406TyrGly: 2.406 ± 0.056	0.932TyrHis: 0.932 ± 0.031	2.552TyrIle: 2.552 ± 0.053	2.197TyrLys: 2.197 ± 0.047	3.815TyrLeu: 3.815 ± 0.063	0.863TyrMet: 0.863 ± 0.026	1.415TyrAsn: 1.415 ± 0.038	1.38TyrPro: 1.38 ± 0.037	1.608TyrGln: 1.608 ± 0.045	1.512TyrArg: 1.512 ± 0.04	2.379TyrSer: 2.379 ± 0.047	1.96TyrThr: 1.96 ± 0.041	2.441TyrVal: 2.441 ± 0.049	0.356TyrTrp: 0.356 ± 0.017	1.487TyrTyr: 1.487 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3944 proteins (1122802 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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