Paenibacillus sp. yr247

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0QEE7|A0A1H0QEE7_9BACL D-aminopeptidase OS=Paenibacillus sp. yr247 OX=1761880 GN=SAMN04487897_14023 PE=3 SV=1
MM1 pKa = 7.46EE2 pKa = 5.25NYY4 pKa = 10.29RR5 pKa = 11.84LFSNWLNQILEE16 pKa = 3.83QDD18 pKa = 3.19IPIGIKK24 pKa = 10.16AFNFNLYY31 pKa = 9.81EE32 pKa = 4.61GSDD35 pKa = 3.71DD36 pKa = 3.94TYY38 pKa = 11.24DD39 pKa = 3.31IQLIGSDD46 pKa = 3.95EE47 pKa = 4.03FDD49 pKa = 4.3EE50 pKa = 5.43NDD52 pKa = 4.18SDD54 pKa = 4.4WACTDD59 pKa = 3.41YY60 pKa = 11.15FNSEE64 pKa = 3.68EE65 pKa = 4.44DD66 pKa = 2.61ICYY69 pKa = 10.08IKK71 pKa = 10.12RR72 pKa = 11.84TEE74 pKa = 5.6DD75 pKa = 3.21IQHH78 pKa = 6.42WEE80 pKa = 3.63QGLNHH85 pKa = 6.0ITMLVEE91 pKa = 4.81RR92 pKa = 11.84YY93 pKa = 9.54LKK95 pKa = 10.44EE96 pKa = 3.89GEE98 pKa = 4.12YY99 pKa = 11.38ANILKK104 pKa = 10.19SASAIGIGFVDD115 pKa = 4.27GDD117 pKa = 3.05IDD119 pKa = 4.46IIFRR123 pKa = 11.84AA124 pKa = 3.91

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0KCQ4|A0A1H0KCQ4_9BACL DUF4357 domain-containing protein OS=Paenibacillus sp. yr247 OX=1761880 GN=SAMN04487897_11656 PE=4 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.8VLQARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6029

0

6029

1757981

12

3124

291.6

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.643 ± 0.033

0.812 ± 0.01

5.015 ± 0.023

6.417 ± 0.038

4.324 ± 0.026

7.121 ± 0.028

2.246 ± 0.017

7.234 ± 0.034

5.866 ± 0.032

10.12 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.852 ± 0.016

4.073 ± 0.022

3.994 ± 0.022

4.02 ± 0.021

4.677 ± 0.026

6.422 ± 0.029

5.469 ± 0.027

7.024 ± 0.026

1.279 ± 0.014

3.392 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski