Switchgrass mosaic-associated virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Switchgrass mosaic-associated virus

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0QTH0|A0A0A0QTH0_9GEMI Movement protein OS=Switchgrass mosaic-associated virus 1 OX=1571533 PE=3 SV=1
MM1 pKa = 7.36SFSSDD6 pKa = 3.48SADD9 pKa = 2.86GGIPNEE15 pKa = 4.0RR16 pKa = 11.84RR17 pKa = 11.84IHH19 pKa = 6.05PGSNFKK25 pKa = 10.53FDD27 pKa = 3.97SNEE30 pKa = 3.36IFLTYY35 pKa = 9.2PRR37 pKa = 11.84CSAEE41 pKa = 3.98PMDD44 pKa = 3.81VGNYY48 pKa = 8.66LWTLLLTLSPKK59 pKa = 10.34YY60 pKa = 10.16VLCTRR65 pKa = 11.84EE66 pKa = 3.53RR67 pKa = 11.84HH68 pKa = 6.01ADD70 pKa = 3.5GTFHH74 pKa = 7.1LHH76 pKa = 7.08AFVQLGYY83 pKa = 11.21NLVTANPHH91 pKa = 5.63HH92 pKa = 7.14LDD94 pKa = 3.17YY95 pKa = 10.84RR96 pKa = 11.84QYY98 pKa = 10.6HH99 pKa = 6.73PNVQPVRR106 pKa = 11.84SSRR109 pKa = 11.84SVRR112 pKa = 11.84DD113 pKa = 3.4YY114 pKa = 11.22CLKK117 pKa = 10.96NPIDD121 pKa = 3.4QYY123 pKa = 10.6TRR125 pKa = 11.84GRR127 pKa = 11.84YY128 pKa = 7.41SARR131 pKa = 11.84TGVRR135 pKa = 11.84NGSTSRR141 pKa = 11.84MGEE144 pKa = 4.0SAADD148 pKa = 3.51WEE150 pKa = 4.89SKK152 pKa = 7.32NQKK155 pKa = 8.57MATIFATATSRR166 pKa = 11.84DD167 pKa = 3.75EE168 pKa = 4.01YY169 pKa = 11.34LSLVKK174 pKa = 8.64TTFPFDD180 pKa = 3.11WATKK184 pKa = 9.22LQAFEE189 pKa = 4.31YY190 pKa = 9.83SASKK194 pKa = 10.44LFPEE198 pKa = 4.59VEE200 pKa = 4.32PEE202 pKa = 4.03YY203 pKa = 9.51EE204 pKa = 4.2TPDD207 pKa = 2.88WATQLRR213 pKa = 11.84CPEE216 pKa = 5.3RR217 pKa = 11.84IADD220 pKa = 3.83WANHH224 pKa = 5.44NVFQTALYY232 pKa = 10.23NIIDD236 pKa = 5.12DD237 pKa = 4.0IPFKK241 pKa = 10.77FCHH244 pKa = 5.82CWKK247 pKa = 10.61SLVGSQKK254 pKa = 11.11DD255 pKa = 3.66YY256 pKa = 11.23IVNPKK261 pKa = 9.59YY262 pKa = 10.75GKK264 pKa = 9.55KK265 pKa = 10.04KK266 pKa = 10.16KK267 pKa = 9.65IAGGIPSIIIVNEE280 pKa = 4.11DD281 pKa = 3.63EE282 pKa = 4.69DD283 pKa = 4.34WLPKK287 pKa = 8.07MTAAQRR293 pKa = 11.84SYY295 pKa = 11.4FEE297 pKa = 4.87ANCEE301 pKa = 3.67VHH303 pKa = 6.36YY304 pKa = 10.4LYY306 pKa = 11.05EE307 pKa = 4.96GDD309 pKa = 4.24SFFAVPEE316 pKa = 3.99QASPDD321 pKa = 3.59LL322 pKa = 4.18

Molecular weight: 36.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0QVW5|A0A0A0QVW5_9GEMI Capsid protein OS=Switchgrass mosaic-associated virus 1 OX=1571533 PE=3 SV=1
MM1 pKa = 7.91PSRR4 pKa = 11.84SRR6 pKa = 11.84YY7 pKa = 6.05TYY9 pKa = 9.99KK10 pKa = 10.48RR11 pKa = 11.84KK12 pKa = 10.07RR13 pKa = 11.84PAKK16 pKa = 9.08RR17 pKa = 11.84ARR19 pKa = 11.84VEE21 pKa = 3.69PWPPEE26 pKa = 3.98LTRR29 pKa = 11.84LGYY32 pKa = 8.39TPRR35 pKa = 11.84DD36 pKa = 3.18TVLRR40 pKa = 11.84GTRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.32GRR48 pKa = 11.84PSLQVINYY56 pKa = 5.56TWKK59 pKa = 9.93NDD61 pKa = 3.0GSAVTIGNGGGVYY74 pKa = 10.79LLTEE78 pKa = 4.87FPRR81 pKa = 11.84GDD83 pKa = 4.24AEE85 pKa = 4.32DD86 pKa = 3.6QRR88 pKa = 11.84HH89 pKa = 4.86TGEE92 pKa = 3.91TLTYY96 pKa = 9.87KK97 pKa = 10.65VSLNLQFAVQEE108 pKa = 4.51DD109 pKa = 3.81YY110 pKa = 11.24RR111 pKa = 11.84KK112 pKa = 10.09YY113 pKa = 10.83CNRR116 pKa = 11.84AQNMVFLVYY125 pKa = 10.29DD126 pKa = 4.17AQPTGTNPKK135 pKa = 8.42ITDD138 pKa = 3.35IFHH141 pKa = 6.72IAASQFFPIPATWMVSRR158 pKa = 11.84EE159 pKa = 3.73NSHH162 pKa = 6.52RR163 pKa = 11.84FVIKK167 pKa = 10.14RR168 pKa = 11.84KK169 pKa = 8.39WMFITEE175 pKa = 4.31VNGSGTAKK183 pKa = 10.55DD184 pKa = 3.89YY185 pKa = 11.55TNAPCYY191 pKa = 8.33PAHH194 pKa = 6.89FSLIFKK200 pKa = 10.46RR201 pKa = 11.84FVQRR205 pKa = 11.84LGVRR209 pKa = 11.84TEE211 pKa = 4.02WKK213 pKa = 10.08NSTEE217 pKa = 4.02GGIGAISKK225 pKa = 10.27GAMYY229 pKa = 10.15LIIAPGNGMPLEE241 pKa = 4.12ARR243 pKa = 11.84GNIRR247 pKa = 11.84LYY249 pKa = 10.5FKK251 pKa = 11.01SIGNQQ256 pKa = 3.0

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

957

99

322

239.3

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.15 ± 0.745

1.567 ± 0.3

4.702 ± 0.657

5.225 ± 0.617

4.598 ± 0.31

6.688 ± 1.235

2.508 ± 0.529

4.493 ± 0.682

4.284 ± 0.665

7.001 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.224

4.807 ± 0.254

6.479 ± 0.39

3.553 ± 0.3

7.315 ± 0.886

7.732 ± 0.871

7.001 ± 0.482

5.016 ± 0.155

2.09 ± 0.047

4.911 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski