Chromatium okenii
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2811 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S7XNB3|A0A2S7XNB3_9GAMM RimK family alpha-L-glutamate ligase OS=Chromatium okenii OX=61644 GN=CXB77_13135 PE=4 SV=1
MM1 pKa = 6.83 FASRR5 pKa = 11.84 AHH7 pKa = 6.18 AVLITDD13 pKa = 4.73 AQLQDD18 pKa = 4.44 LDD20 pKa = 3.85 QLLAGLDD27 pKa = 3.5 ADD29 pKa = 3.99 VEE31 pKa = 4.38 PWLVASNQDD40 pKa = 3.28 AMPLIFKK47 pKa = 10.64 ALAHH51 pKa = 6.95 PDD53 pKa = 3.39 LTQLHH58 pKa = 6.79 LLAHH62 pKa = 6.69 GAPGEE67 pKa = 3.96 IRR69 pKa = 11.84 LGEE72 pKa = 4.17 RR73 pKa = 11.84 ALTAADD79 pKa = 3.6 FRR81 pKa = 11.84 QYY83 pKa = 11.5 LNGAAMRR90 pKa = 11.84 DD91 pKa = 3.42 LSINFWSCHH100 pKa = 4.94 TGAGEE105 pKa = 3.87 MGTEE109 pKa = 4.24 FTHH112 pKa = 6.96 AVAAATGARR121 pKa = 11.84 VLAAEE126 pKa = 4.31 GLIGAANKK134 pKa = 9.52 QGSWEE139 pKa = 4.23 LNGITAPFSEE149 pKa = 4.82 TARR152 pKa = 11.84 AGYY155 pKa = 11.06 ANVLAVRR162 pKa = 11.84 DD163 pKa = 3.86 RR164 pKa = 11.84 FEE166 pKa = 6.41 DD167 pKa = 3.39 NDD169 pKa = 3.98 TAATATNLGTITSTRR184 pKa = 11.84 TEE186 pKa = 3.99 TGLSIEE192 pKa = 4.66 TNDD195 pKa = 3.85 PDD197 pKa = 3.39 WFQFTLGSTGTSASNVAIDD216 pKa = 3.96 FSNALGDD223 pKa = 4.36 LDD225 pKa = 4.21 IFLYY229 pKa = 10.76 DD230 pKa = 3.32 AAGNALQGTYY240 pKa = 10.42 GSNDD244 pKa = 3.22 HH245 pKa = 6.04 EE246 pKa = 4.61 QISLSGLAAGSYY258 pKa = 9.66 LIKK261 pKa = 10.85 VRR263 pKa = 11.84 DD264 pKa = 3.6 WTSLDD269 pKa = 3.22 GGEE272 pKa = 4.28 NPNYY276 pKa = 9.45 TLTLIGSGEE285 pKa = 4.03 VSPTVSYY292 pKa = 7.64 PTLDD296 pKa = 3.51 YY297 pKa = 10.98 MPDD300 pKa = 3.37 LTSSQMSGARR310 pKa = 11.84 SLTFAPYY317 pKa = 10.25 TSTSWDD323 pKa = 3.59 SKK325 pKa = 9.78 AGAYY329 pKa = 10.06 GSEE332 pKa = 4.46 SGASSHH338 pKa = 7.05 SIYY341 pKa = 10.43 TITATAGQVYY351 pKa = 10.44 DD352 pKa = 5.02 LYY354 pKa = 11.03 SVSYY358 pKa = 9.78 FDD360 pKa = 4.79 PYY362 pKa = 11.5 SLTVYY367 pKa = 10.59 DD368 pKa = 4.15 SLGNAIVTNAEE379 pKa = 3.69 SDD381 pKa = 3.82 DD382 pKa = 3.94 PADD385 pKa = 3.8 YY386 pKa = 11.43 NLGDD390 pKa = 3.77 AYY392 pKa = 11.47 YY393 pKa = 10.88 GVDD396 pKa = 3.93 WLNDD400 pKa = 2.87 WTAPYY405 pKa = 10.47 SGTYY409 pKa = 9.58 YY410 pKa = 10.93 VDD412 pKa = 4.35 ASWHH416 pKa = 5.08 QGSYY420 pKa = 7.75 FTFYY424 pKa = 11.62 DD425 pKa = 3.9 LMIYY429 pKa = 9.93 KK430 pKa = 10.29 NKK432 pKa = 9.06 STTVLPTLSLSSLSPSSVSEE452 pKa = 4.22 GNSGSTSMMVNVTRR466 pKa = 11.84 TGDD469 pKa = 3.45 LSSTSSASWSVVGAAATASDD489 pKa = 4.27 FLSGVLPTGTVNFAAGQASATATINIAGDD518 pKa = 3.46 TTIEE522 pKa = 3.84 SDD524 pKa = 3.76 EE525 pKa = 4.39 NFTVTLSNPVSATLDD540 pKa = 3.16 ISSATGTIVNDD551 pKa = 3.98 DD552 pKa = 3.61 VSPPVPYY559 pKa = 8.19 PTLDD563 pKa = 3.75 YY564 pKa = 11.1 IPDD567 pKa = 3.97 LKK569 pKa = 10.8 SSQMYY574 pKa = 9.81 EE575 pKa = 3.75 ADD577 pKa = 4.08 LLAFLPVTSSVYY589 pKa = 10.47 YY590 pKa = 10.92 DD591 pKa = 3.43 MEE593 pKa = 6.61 AYY595 pKa = 10.15 DD596 pKa = 4.9 YY597 pKa = 11.29 GLTSNASLHH606 pKa = 6.44 SLYY609 pKa = 10.62 QFNATTGVLYY619 pKa = 10.32 DD620 pKa = 4.35 IYY622 pKa = 10.65 SISYY626 pKa = 10.41 LDD628 pKa = 3.63 PTEE631 pKa = 3.93 LTIYY635 pKa = 10.41 DD636 pKa = 3.6 QFGNAIAINNEE647 pKa = 3.52 SDD649 pKa = 4.64 DD650 pKa = 4.42 PDD652 pKa = 3.7 NSTLSWVDD660 pKa = 3.36 DD661 pKa = 3.99 GGSYY665 pKa = 10.79 SFDD668 pKa = 5.43 AIWEE672 pKa = 3.95 WSAPYY677 pKa = 10.47 SGTFYY682 pKa = 11.24 VDD684 pKa = 3.34 SGWNQGSFFTYY695 pKa = 9.14 WDD697 pKa = 3.25 VSVYY701 pKa = 10.25 RR702 pKa = 11.84 NKK704 pKa = 9.91 STTPSLPTLSLSSLTPTSISEE725 pKa = 4.32 GNSGYY730 pKa = 9.0 TPITFTVNRR739 pKa = 11.84 DD740 pKa = 2.9 GDD742 pKa = 3.73 LSGTSRR748 pKa = 11.84 VFFAVTGTGTNPVNAADD765 pKa = 4.08 FRR767 pKa = 11.84 DD768 pKa = 4.06 GVLPSDD774 pKa = 4.47 WIDD777 pKa = 3.57 FDD779 pKa = 4.13 SGAVSATITSYY790 pKa = 11.3 VVAGDD795 pKa = 3.59 TTPEE799 pKa = 3.95 PNEE802 pKa = 4.03 NFTVTLSSPTGATLGTSTSVIGTITNDD829 pKa = 3.6 DD830 pKa = 4.35 SLPLPTISLSPLSPSIITEE849 pKa = 3.91 GHH851 pKa = 5.52 SGYY854 pKa = 8.97 TPVNIVVTRR863 pKa = 11.84 GGDD866 pKa = 3.41 LSSTSSATWTVVGTGVAPVTADD888 pKa = 3.45 DD889 pKa = 4.54 FGGFLPSGIISLAAGVATATVSINILGDD917 pKa = 3.39 TTVEE921 pKa = 4.18 SNEE924 pKa = 4.54 DD925 pKa = 3.49 FTVMLSNPSGATLIPNTVTGTITNDD950 pKa = 3.32 DD951 pKa = 4.28 VILPTLSLASLTPSIIMEE969 pKa = 4.48 GNSGFTPMTVTITRR983 pKa = 11.84 SGDD986 pKa = 3.4 LSGTSAANWSVIGAGATADD1005 pKa = 3.81 DD1006 pKa = 4.95 FIGGVLPSGTISFPAWQATTTATINIFGDD1035 pKa = 3.62 TLVEE1039 pKa = 4.38 SNEE1042 pKa = 3.95 NFSVVLAAPVGATLGTSTSAMGTINNDD1069 pKa = 3.1 DD1070 pKa = 4.1 TLPTINVAPVYY1081 pKa = 10.55 AITEE1085 pKa = 4.44 GNSGSTTVNVTVNRR1099 pKa = 11.84 TGDD1102 pKa = 3.34 LSTASSANWSVAGSGTTPAIGADD1125 pKa = 3.59 FTGGVLPTGTVNFAAGIATASIAINVAGDD1154 pKa = 3.59 TVFEE1158 pKa = 4.22 QNEE1161 pKa = 4.22 NFTVSLNTPVNATLGASVNTTGTINNDD1188 pKa = 2.92 DD1189 pKa = 4.27 TLPVLNVAPVLAITEE1204 pKa = 4.43 GNSGSTAVNITVNRR1218 pKa = 11.84 TGDD1221 pKa = 3.44 LSTASSTNWSVAGSGTNPVIGADD1244 pKa = 3.57 FTGGVLPTGTVNFAAGVATATIAINVAGDD1273 pKa = 3.43 TTFEE1277 pKa = 4.44 PNEE1280 pKa = 4.32 DD1281 pKa = 3.49 FTVSLNTPVNATLDD1295 pKa = 3.54 ASVNTTGTINNDD1307 pKa = 2.92 DD1308 pKa = 4.27 TLPVLNVAPVLAITEE1323 pKa = 4.43 GNSGSTAVNVTVNRR1337 pKa = 11.84 TGDD1340 pKa = 3.34 LSTASSANWSVAGSGTNPVIGADD1363 pKa = 3.62 FMGGVLPMGTVNFAAGLATATIAINVAGDD1392 pKa = 3.43 TTFEE1396 pKa = 4.44 PNEE1399 pKa = 4.32 DD1400 pKa = 3.49 FTVSLNTPVNATLDD1414 pKa = 3.54 ASVNTTGTINNDD1426 pKa = 2.92 DD1427 pKa = 4.27 TLPVLNVAPVLAITEE1442 pKa = 4.43 GNSGSTAVNVTVNRR1456 pKa = 11.84 TGDD1459 pKa = 3.34 LSTASSANWSVAGSGTNPAIGADD1482 pKa = 3.7 FTGGVLPTGMVNFAAGIATASIAINVAGDD1511 pKa = 3.59 TVFEE1515 pKa = 4.22 QNEE1518 pKa = 4.22 NFTVSLNTPVNATLGSSVNTTGTINNDD1545 pKa = 2.92 DD1546 pKa = 4.27 TLPVLNVAPVLAITEE1561 pKa = 4.43 GNSGSTAVNITVNRR1575 pKa = 11.84 TGDD1578 pKa = 3.36 LSTASSANWSVAGSGTNPVIGADD1601 pKa = 3.57 FTGGVLPTGTVNFAAGVATATIAINVAGDD1630 pKa = 3.43 TLFEE1634 pKa = 4.32 QNEE1637 pKa = 4.25 NFTVSLNTPVNATLGTSTSAMGTINNDD1664 pKa = 2.95 DD1665 pKa = 4.23 SIAIVGTSNNDD1676 pKa = 3.34 RR1677 pKa = 11.84 LKK1679 pKa = 10.71 GTIKK1683 pKa = 10.4 SDD1685 pKa = 3.58 QLDD1688 pKa = 3.75 GLAGDD1693 pKa = 5.21 DD1694 pKa = 3.9 YY1695 pKa = 11.65 FFGLGGNDD1703 pKa = 3.66 TLNGGAGNDD1712 pKa = 3.59 TMRR1715 pKa = 11.84 GGTGLDD1721 pKa = 3.49 SMIGGDD1727 pKa = 4.77 GNDD1730 pKa = 3.19 LYY1732 pKa = 11.37 YY1733 pKa = 10.65 VEE1735 pKa = 5.42 EE1736 pKa = 5.26 IGDD1739 pKa = 3.88 IVVEE1743 pKa = 4.27 TATGGIDD1750 pKa = 3.45 TVNSSLNNYY1759 pKa = 7.0 MLNAAVEE1766 pKa = 4.26 NGRR1769 pKa = 11.84 ISIPGTASITGNDD1782 pKa = 3.67 LDD1784 pKa = 4.91 NILFAGVGNNTIDD1797 pKa = 3.7 GGTGTDD1803 pKa = 3.15 TVVYY1807 pKa = 9.77 SALKK1811 pKa = 8.87 TSVGSTLGVKK1821 pKa = 9.92 VNLMNTAIQITGSSGSDD1838 pKa = 2.99 LLLSIEE1844 pKa = 4.53 NLTGSNKK1851 pKa = 9.84 NDD1853 pKa = 3.56 TLGGNSAANVLNGGLGNDD1871 pKa = 3.76 ILTGGAGKK1879 pKa = 10.23 DD1880 pKa = 3.11 VFVFDD1885 pKa = 3.5 TALNRR1890 pKa = 11.84 NVDD1893 pKa = 3.74 QIKK1896 pKa = 10.67 DD1897 pKa = 3.58 FSVVDD1902 pKa = 3.58 DD1903 pKa = 4.92 TIQLEE1908 pKa = 4.17 NSVFKK1913 pKa = 10.78 QLSSTGSLKK1922 pKa = 10.76 ASYY1925 pKa = 9.44 FVKK1928 pKa = 9.85 NTTGKK1933 pKa = 10.79 ANDD1936 pKa = 3.59 TDD1938 pKa = 4.54 DD1939 pKa = 5.21 FIIYY1943 pKa = 8.16 DD1944 pKa = 3.98 TDD1946 pKa = 3.63 SGALFYY1952 pKa = 11.19 DD1953 pKa = 3.96 ADD1955 pKa = 4.01 GSGAGAAVQFAIVSIGVVLTAADD1978 pKa = 4.96 FIVSS1982 pKa = 3.71
Molecular weight: 203.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.541
IPC_protein 3.617
Toseland 3.363
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.274
Solomon 3.617
Lehninger 3.567
Nozaki 3.719
DTASelect 4.024
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.732
Patrickios 0.922
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|A0A2S7XQK1|A0A2S7XQK1_9GAMM 50S ribosomal protein L14 OS=Chromatium okenii OX=61644 GN=rplN PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LKK11 pKa = 10.48 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 SATRR25 pKa = 11.84 NGLKK29 pKa = 10.3 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.29 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSPVV45 pKa = 2.69
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2811
0
2811
814741
27
2927
289.8
31.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.737 ± 0.068
1.148 ± 0.027
5.518 ± 0.039
5.656 ± 0.05
3.767 ± 0.036
6.88 ± 0.06
2.311 ± 0.028
5.922 ± 0.04
3.571 ± 0.045
10.952 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.027
3.856 ± 0.056
4.67 ± 0.051
4.471 ± 0.044
6.261 ± 0.065
5.505 ± 0.054
6.073 ± 0.087
6.686 ± 0.044
1.316 ± 0.022
2.479 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here