Staphylococcus phage vB_SauH_DELF3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Silviavirus; unclassified Silviavirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G1KWH1|A0A7G1KWH1_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SauH_DELF3 OX=2766478 PE=4 SV=1
MM1 pKa = 7.95PNTSKK6 pKa = 10.95EE7 pKa = 4.06FLQPRR12 pKa = 11.84SPSTISDD19 pKa = 3.57DD20 pKa = 3.54AFSGGEE26 pKa = 4.0SGKK29 pKa = 10.79SGEE32 pKa = 4.35LNCNKK37 pKa = 10.47DD38 pKa = 2.93IVPFFNNYY46 pKa = 8.58EE47 pKa = 3.89QDD49 pKa = 3.58FEE51 pKa = 4.5NTVVNFPGEE60 pKa = 4.08AKK62 pKa = 10.44EE63 pKa = 3.74ITLYY67 pKa = 10.8DD68 pKa = 3.8INNMEE73 pKa = 4.33TFEE76 pKa = 4.63ILNNHH81 pKa = 5.76YY82 pKa = 10.54NGEE85 pKa = 4.19LATDD89 pKa = 3.83VANIQAIPEE98 pKa = 4.22IATEE102 pKa = 3.99MAEE105 pKa = 4.02DD106 pKa = 3.8VSVDD110 pKa = 3.23EE111 pKa = 4.79WPDD114 pKa = 3.11MDD116 pKa = 4.15EE117 pKa = 5.1DD118 pKa = 3.85EE119 pKa = 5.11RR120 pKa = 11.84DD121 pKa = 3.24EE122 pKa = 5.25HH123 pKa = 8.71IYY125 pKa = 11.25DD126 pKa = 3.66SMDD129 pKa = 3.31SVEE132 pKa = 4.53YY133 pKa = 10.93DD134 pKa = 3.77CATQDD139 pKa = 3.34KK140 pKa = 10.4IAIVKK145 pKa = 9.58LAVPVVAQYY154 pKa = 11.62IMVEE158 pKa = 4.14MEE160 pKa = 4.07TLEE163 pKa = 4.53SFDD166 pKa = 3.52SVEE169 pKa = 4.18RR170 pKa = 11.84FTDD173 pKa = 2.9IDD175 pKa = 3.7EE176 pKa = 6.03DD177 pKa = 3.97INEE180 pKa = 4.24MDD182 pKa = 4.29SKK184 pKa = 10.27QTTEE188 pKa = 5.05GSDD191 pKa = 3.14KK192 pKa = 11.2DD193 pKa = 3.26AGLIMKK199 pKa = 9.41YY200 pKa = 10.75NSLPEE205 pKa = 4.28LGIDD209 pKa = 3.96YY210 pKa = 10.0DD211 pKa = 4.06ATTLGPNVIDD221 pKa = 4.04EE222 pKa = 4.85SLNKK226 pKa = 10.22

Molecular weight: 25.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G1KX03|A0A7G1KX03_9CAUD DNA helicase OS=Staphylococcus phage vB_SauH_DELF3 OX=2766478 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 4.7HH3 pKa = 5.46VQRR6 pKa = 11.84LFRR9 pKa = 11.84IGDD12 pKa = 3.64GSKK15 pKa = 9.37ATSDD19 pKa = 2.63WWKK22 pKa = 11.11KK23 pKa = 6.21NTHH26 pKa = 7.13PILIGITNSLRR37 pKa = 11.84TYY39 pKa = 10.04RR40 pKa = 11.84KK41 pKa = 9.22VVSRR45 pKa = 11.84NNN47 pKa = 3.14

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

177

0

177

36445

38

1043

205.9

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.401 ± 0.169

1.246 ± 0.089

6.766 ± 0.131

6.506 ± 0.196

3.69 ± 0.127

5.49 ± 0.222

2.085 ± 0.108

6.805 ± 0.176

8.594 ± 0.189

8.369 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.078

6.541 ± 0.178

3.564 ± 0.153

3.295 ± 0.147

4.719 ± 0.128

7.12 ± 0.143

6.473 ± 0.215

6.522 ± 0.179

0.862 ± 0.055

4.799 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski