Amino acid dipepetide frequency for Clostridium sp. (strain SY8519)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.137AlaAla: 10.137 ± 0.205	1.367AlaCys: 1.367 ± 0.046	5.491AlaAsp: 5.491 ± 0.086	6.773AlaGlu: 6.773 ± 0.121	3.069AlaPhe: 3.069 ± 0.067	7.488AlaGly: 7.488 ± 0.122	1.4AlaHis: 1.4 ± 0.042	4.731AlaIle: 4.731 ± 0.088	4.551AlaLys: 4.551 ± 0.073	7.319AlaLeu: 7.319 ± 0.103	2.503AlaMet: 2.503 ± 0.061	2.354AlaAsn: 2.354 ± 0.048	2.367AlaPro: 2.367 ± 0.054	2.646AlaGln: 2.646 ± 0.059	4.225AlaArg: 4.225 ± 0.096	4.719AlaSer: 4.719 ± 0.094	2.961AlaThr: 2.961 ± 0.063	7.305AlaVal: 7.305 ± 0.103	0.771AlaTrp: 0.771 ± 0.035	2.945AlaTyr: 2.945 ± 0.065	0.0AlaXaa: 0.0 ± 0.0
Cys	1.214CysAla: 1.214 ± 0.042	0.306CysCys: 0.306 ± 0.02	0.789CysAsp: 0.789 ± 0.033	0.85CysGlu: 0.85 ± 0.036	0.618CysPhe: 0.618 ± 0.028	1.686CysGly: 1.686 ± 0.059	0.34CysHis: 0.34 ± 0.02	1.032CysIle: 1.032 ± 0.038	0.614CysLys: 0.614 ± 0.025	1.157CysLeu: 1.157 ± 0.039	0.519CysMet: 0.519 ± 0.027	0.471CysAsn: 0.471 ± 0.021	0.779CysPro: 0.779 ± 0.033	0.418CysGln: 0.418 ± 0.023	1.098CysArg: 1.098 ± 0.039	0.99CysSer: 0.99 ± 0.035	0.831CysThr: 0.831 ± 0.035	1.079CysVal: 1.079 ± 0.034	0.123CysTrp: 0.123 ± 0.011	0.58CysTyr: 0.58 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.815AspAla: 4.815 ± 0.08	0.909AspCys: 0.909 ± 0.035	2.594AspAsp: 2.594 ± 0.065	3.726AspGlu: 3.726 ± 0.081	2.401AspPhe: 2.401 ± 0.048	4.41AspGly: 4.41 ± 0.08	1.122AspHis: 1.122 ± 0.041	3.868AspIle: 3.868 ± 0.07	2.547AspLys: 2.547 ± 0.071	5.304AspLeu: 5.304 ± 0.084	1.546AspMet: 1.546 ± 0.043	1.711AspAsn: 1.711 ± 0.053	2.467AspPro: 2.467 ± 0.058	1.754AspGln: 1.754 ± 0.053	3.472AspArg: 3.472 ± 0.073	3.333AspSer: 3.333 ± 0.075	3.405AspThr: 3.405 ± 0.064	3.54AspVal: 3.54 ± 0.067	0.572AspTrp: 0.572 ± 0.027	2.6AspTyr: 2.6 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	6.195GluAla: 6.195 ± 0.122	0.713GluCys: 0.713 ± 0.031	4.076GluAsp: 4.076 ± 0.082	6.987GluGlu: 6.987 ± 0.139	2.398GluPhe: 2.398 ± 0.058	4.284GluGly: 4.284 ± 0.092	1.494GluHis: 1.494 ± 0.042	5.227GluIle: 5.227 ± 0.092	5.673GluLys: 5.673 ± 0.1	6.525GluLeu: 6.525 ± 0.11	2.28GluMet: 2.28 ± 0.06	3.398GluAsn: 3.398 ± 0.058	1.962GluPro: 1.962 ± 0.053	3.243GluGln: 3.243 ± 0.069	3.48GluArg: 3.48 ± 0.069	3.185GluSer: 3.185 ± 0.053	3.915GluThr: 3.915 ± 0.073	4.041GluVal: 4.041 ± 0.083	0.584GluTrp: 0.584 ± 0.03	2.711GluTyr: 2.711 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	3.184PheAla: 3.184 ± 0.071	0.769PheCys: 0.769 ± 0.028	2.361PheAsp: 2.361 ± 0.056	2.267PheGlu: 2.267 ± 0.056	1.682PhePhe: 1.682 ± 0.056	2.989PheGly: 2.989 ± 0.069	1.044PheHis: 1.044 ± 0.038	2.164PheIle: 2.164 ± 0.055	1.429PheLys: 1.429 ± 0.044	3.791PheLeu: 3.791 ± 0.087	0.931PheMet: 0.931 ± 0.032	1.157PheAsn: 1.157 ± 0.04	1.517PhePro: 1.517 ± 0.04	1.273PheGln: 1.273 ± 0.045	2.271PheArg: 2.271 ± 0.06	2.693PheSer: 2.693 ± 0.063	2.08PheThr: 2.08 ± 0.05	2.496PheVal: 2.496 ± 0.059	0.391PheTrp: 0.391 ± 0.023	1.476PheTyr: 1.476 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.791GlyAla: 5.791 ± 0.106	1.421GlyCys: 1.421 ± 0.051	3.508GlyAsp: 3.508 ± 0.067	4.396GlyGlu: 4.396 ± 0.084	3.013GlyPhe: 3.013 ± 0.069	5.306GlyGly: 5.306 ± 0.107	1.468GlyHis: 1.468 ± 0.047	6.291GlyIle: 6.291 ± 0.095	5.309GlyLys: 5.309 ± 0.085	5.946GlyLeu: 5.946 ± 0.086	2.523GlyMet: 2.523 ± 0.068	2.821GlyAsn: 2.821 ± 0.065	1.649GlyPro: 1.649 ± 0.05	2.241GlyGln: 2.241 ± 0.052	4.306GlyArg: 4.306 ± 0.079	4.71GlySer: 4.71 ± 0.092	5.086GlyThr: 5.086 ± 0.105	4.959GlyVal: 4.959 ± 0.093	0.777GlyTrp: 0.777 ± 0.034	3.06GlyTyr: 3.06 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.51HisAla: 1.51 ± 0.05	0.4HisCys: 0.4 ± 0.022	0.9HisAsp: 0.9 ± 0.036	1.12HisGlu: 1.12 ± 0.044	0.832HisPhe: 0.832 ± 0.03	1.518HisGly: 1.518 ± 0.046	0.499HisHis: 0.499 ± 0.029	1.326HisIle: 1.326 ± 0.041	0.885HisLys: 0.885 ± 0.035	1.777HisLeu: 1.777 ± 0.045	0.737HisMet: 0.737 ± 0.034	0.645HisAsn: 0.645 ± 0.029	1.231HisPro: 1.231 ± 0.04	0.661HisGln: 0.661 ± 0.025	1.187HisArg: 1.187 ± 0.044	1.148HisSer: 1.148 ± 0.039	1.31HisThr: 1.31 ± 0.043	1.319HisVal: 1.319 ± 0.04	0.19HisTrp: 0.19 ± 0.015	0.763HisTyr: 0.763 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.94IleAla: 5.94 ± 0.092	1.326IleCys: 1.326 ± 0.045	3.708IleAsp: 3.708 ± 0.071	3.802IleGlu: 3.802 ± 0.077	2.582IlePhe: 2.582 ± 0.062	5.118IleGly: 5.118 ± 0.085	1.603IleHis: 1.603 ± 0.048	4.254IleIle: 4.254 ± 0.084	2.865IleLys: 2.865 ± 0.064	6.559IleLeu: 6.559 ± 0.105	1.732IleMet: 1.732 ± 0.049	2.352IleAsn: 2.352 ± 0.054	3.245IlePro: 3.245 ± 0.069	2.192IleGln: 2.192 ± 0.05	5.157IleArg: 5.157 ± 0.094	4.437IleSer: 4.437 ± 0.081	3.87IleThr: 3.87 ± 0.08	4.229IleVal: 4.229 ± 0.081	0.55IleTrp: 0.55 ± 0.023	2.449IleTyr: 2.449 ± 0.056	0.0IleXaa: 0.0 ± 0.0
Lys	4.931LysAla: 4.931 ± 0.1	0.606LysCys: 0.606 ± 0.029	3.564LysAsp: 3.564 ± 0.08	5.472LysGlu: 5.472 ± 0.103	1.532LysPhe: 1.532 ± 0.043	3.87LysGly: 3.87 ± 0.074	0.925LysHis: 0.925 ± 0.037	4.003LysIle: 4.003 ± 0.077	5.692LysLys: 5.692 ± 0.116	4.395LysLeu: 4.395 ± 0.073	1.691LysMet: 1.691 ± 0.046	2.96LysAsn: 2.96 ± 0.066	2.029LysPro: 2.029 ± 0.052	2.23LysGln: 2.23 ± 0.056	2.871LysArg: 2.871 ± 0.066	2.982LysSer: 2.982 ± 0.069	3.839LysThr: 3.839 ± 0.088	3.676LysVal: 3.676 ± 0.078	0.454LysTrp: 0.454 ± 0.027	2.638LysTyr: 2.638 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	7.371LeuAla: 7.371 ± 0.117	1.398LeuCys: 1.398 ± 0.042	4.909LeuAsp: 4.909 ± 0.083	5.802LeuGlu: 5.802 ± 0.117	3.495LeuPhe: 3.495 ± 0.081	5.667LeuGly: 5.667 ± 0.096	1.723LeuHis: 1.723 ± 0.043	5.731LeuIle: 5.731 ± 0.087	5.613LeuLys: 5.613 ± 0.102	8.259LeuLeu: 8.259 ± 0.151	2.51LeuMet: 2.51 ± 0.053	3.342LeuAsn: 3.342 ± 0.065	3.823LeuPro: 3.823 ± 0.072	3.147LeuGln: 3.147 ± 0.057	4.362LeuArg: 4.362 ± 0.093	5.778LeuSer: 5.778 ± 0.091	5.537LeuThr: 5.537 ± 0.089	5.208LeuVal: 5.208 ± 0.087	0.643LeuTrp: 0.643 ± 0.034	3.035LeuTyr: 3.035 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	2.413MetAla: 2.413 ± 0.062	0.374MetCys: 0.374 ± 0.02	1.83MetAsp: 1.83 ± 0.05	2.385MetGlu: 2.385 ± 0.06	0.906MetPhe: 0.906 ± 0.031	2.076MetGly: 2.076 ± 0.048	0.586MetHis: 0.586 ± 0.03	2.007MetIle: 2.007 ± 0.05	2.34MetLys: 2.34 ± 0.049	2.565MetLeu: 2.565 ± 0.059	0.859MetMet: 0.859 ± 0.036	1.412MetAsn: 1.412 ± 0.041	1.162MetPro: 1.162 ± 0.04	1.057MetGln: 1.057 ± 0.036	1.48MetArg: 1.48 ± 0.048	1.638MetSer: 1.638 ± 0.044	1.83MetThr: 1.83 ± 0.046	1.663MetVal: 1.663 ± 0.051	0.171MetTrp: 0.171 ± 0.013	0.86MetTyr: 0.86 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	2.823AsnAla: 2.823 ± 0.062	0.603AsnCys: 0.603 ± 0.028	1.811AsnAsp: 1.811 ± 0.053	2.118AsnGlu: 2.118 ± 0.047	1.301AsnPhe: 1.301 ± 0.043	3.271AsnGly: 3.271 ± 0.063	0.886AsnHis: 0.886 ± 0.039	2.752AsnIle: 2.752 ± 0.056	1.874AsnLys: 1.874 ± 0.05	3.234AsnLeu: 3.234 ± 0.065	1.098AsnMet: 1.098 ± 0.032	1.394AsnAsn: 1.394 ± 0.051	1.995AsnPro: 1.995 ± 0.053	1.402AsnGln: 1.402 ± 0.05	2.4AsnArg: 2.4 ± 0.051	1.978AsnSer: 1.978 ± 0.051	2.14AsnThr: 2.14 ± 0.06	2.324AsnVal: 2.324 ± 0.053	0.42AsnTrp: 0.42 ± 0.024	1.65AsnTyr: 1.65 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	3.322ProAla: 3.322 ± 0.068	0.489ProCys: 0.489 ± 0.029	2.805ProAsp: 2.805 ± 0.063	4.127ProGlu: 4.127 ± 0.082	1.512ProPhe: 1.512 ± 0.039	2.965ProGly: 2.965 ± 0.059	0.648ProHis: 0.648 ± 0.023	2.045ProIle: 2.045 ± 0.052	2.031ProLys: 2.031 ± 0.05	2.841ProLeu: 2.841 ± 0.058	0.94ProMet: 0.94 ± 0.035	1.24ProAsn: 1.24 ± 0.041	0.891ProPro: 0.891 ± 0.037	1.136ProGln: 1.136 ± 0.039	1.364ProArg: 1.364 ± 0.038	2.027ProSer: 2.027 ± 0.055	1.478ProThr: 1.478 ± 0.038	3.233ProVal: 3.233 ± 0.059	0.328ProTrp: 0.328 ± 0.019	1.411ProTyr: 1.411 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	3.101GlnAla: 3.101 ± 0.071	0.341GlnCys: 0.341 ± 0.019	1.636GlnAsp: 1.636 ± 0.048	2.791GlnGlu: 2.791 ± 0.068	1.111GlnPhe: 1.111 ± 0.037	2.001GlnGly: 2.001 ± 0.056	0.586GlnHis: 0.586 ± 0.027	2.724GlnIle: 2.724 ± 0.048	2.72GlnLys: 2.72 ± 0.061	2.957GlnLeu: 2.957 ± 0.068	1.316GlnMet: 1.316 ± 0.041	1.476GlnAsn: 1.476 ± 0.036	1.095GlnPro: 1.095 ± 0.036	1.38GlnGln: 1.38 ± 0.045	1.579GlnArg: 1.579 ± 0.049	1.615GlnSer: 1.615 ± 0.046	2.076GlnThr: 2.076 ± 0.058	2.219GlnVal: 2.219 ± 0.048	0.302GlnTrp: 0.302 ± 0.018	1.28GlnTyr: 1.28 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	3.653ArgAla: 3.653 ± 0.072	0.82ArgCys: 0.82 ± 0.034	2.737ArgAsp: 2.737 ± 0.061	4.729ArgGlu: 4.729 ± 0.091	2.172ArgPhe: 2.172 ± 0.056	3.255ArgGly: 3.255 ± 0.066	1.111ArgHis: 1.111 ± 0.042	4.348ArgIle: 4.348 ± 0.078	4.158ArgLys: 4.158 ± 0.079	4.665ArgLeu: 4.665 ± 0.077	1.95ArgMet: 1.95 ± 0.053	2.317ArgAsn: 2.317 ± 0.062	1.931ArgPro: 1.931 ± 0.059	2.512ArgGln: 2.512 ± 0.064	3.733ArgArg: 3.733 ± 0.096	3.036ArgSer: 3.036 ± 0.065	2.942ArgThr: 2.942 ± 0.067	3.172ArgVal: 3.172 ± 0.064	0.517ArgTrp: 0.517 ± 0.028	2.18ArgTyr: 2.18 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	5.214SerAla: 5.214 ± 0.1	0.93SerCys: 0.93 ± 0.033	3.31SerAsp: 3.31 ± 0.068	3.82SerGlu: 3.82 ± 0.062	2.332SerPhe: 2.332 ± 0.055	5.78SerGly: 5.78 ± 0.1	1.138SerHis: 1.138 ± 0.039	3.541SerIle: 3.541 ± 0.072	2.901SerLys: 2.901 ± 0.07	4.872SerLeu: 4.872 ± 0.074	1.818SerMet: 1.818 ± 0.051	2.016SerAsn: 2.016 ± 0.054	1.859SerPro: 1.859 ± 0.055	1.671SerGln: 1.671 ± 0.041	3.693SerArg: 3.693 ± 0.07	4.06SerSer: 4.06 ± 0.121	2.656SerThr: 2.656 ± 0.069	4.325SerVal: 4.325 ± 0.072	0.556SerTrp: 0.556 ± 0.028	2.298SerTyr: 2.298 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	5.549ThrAla: 5.549 ± 0.102	0.743ThrCys: 0.743 ± 0.03	3.657ThrAsp: 3.657 ± 0.079	4.144ThrGlu: 4.144 ± 0.082	2.042ThrPhe: 2.042 ± 0.056	5.332ThrGly: 5.332 ± 0.09	0.996ThrHis: 0.996 ± 0.036	3.667ThrIle: 3.667 ± 0.067	2.673ThrLys: 2.673 ± 0.071	4.493ThrLeu: 4.493 ± 0.085	1.41ThrMet: 1.41 ± 0.039	1.885ThrAsn: 1.885 ± 0.056	2.281ThrPro: 2.281 ± 0.053	1.405ThrGln: 1.405 ± 0.037	2.536ThrArg: 2.536 ± 0.057	2.942ThrSer: 2.942 ± 0.074	2.51ThrThr: 2.51 ± 0.067	4.75ThrVal: 4.75 ± 0.098	0.495ThrTrp: 0.495 ± 0.022	2.117ThrTyr: 2.117 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	4.522ValAla: 4.522 ± 0.08	1.272ValCys: 1.272 ± 0.037	3.492ValAsp: 3.492 ± 0.066	3.879ValGlu: 3.879 ± 0.073	2.992ValPhe: 2.992 ± 0.069	3.902ValGly: 3.902 ± 0.079	1.255ValHis: 1.255 ± 0.04	5.289ValIle: 5.289 ± 0.087	3.892ValLys: 3.892 ± 0.087	6.252ValLeu: 6.252 ± 0.111	2.004ValMet: 2.004 ± 0.05	2.553ValAsn: 2.553 ± 0.056	2.772ValPro: 2.772 ± 0.058	2.035ValGln: 2.035 ± 0.048	3.821ValArg: 3.821 ± 0.064	4.783ValSer: 4.783 ± 0.089	4.464ValThr: 4.464 ± 0.101	4.18ValVal: 4.18 ± 0.084	0.588ValTrp: 0.588 ± 0.027	2.613ValTyr: 2.613 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.566TrpAla: 0.566 ± 0.027	0.12TrpCys: 0.12 ± 0.013	0.503TrpAsp: 0.503 ± 0.025	0.613TrpGlu: 0.613 ± 0.03	0.419TrpPhe: 0.419 ± 0.024	0.598TrpGly: 0.598 ± 0.031	0.193TrpHis: 0.193 ± 0.017	0.661TrpIle: 0.661 ± 0.028	0.802TrpLys: 0.802 ± 0.036	0.789TrpLeu: 0.789 ± 0.031	0.301TrpMet: 0.301 ± 0.02	0.509TrpAsn: 0.509 ± 0.028	0.214TrpPro: 0.214 ± 0.016	0.323TrpGln: 0.323 ± 0.019	0.382TrpArg: 0.382 ± 0.02	0.48TrpSer: 0.48 ± 0.027	0.426TrpThr: 0.426 ± 0.027	0.495TrpVal: 0.495 ± 0.027	0.113TrpTrp: 0.113 ± 0.012	0.367TrpTyr: 0.367 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.872TyrAla: 2.872 ± 0.073	0.586TyrCys: 0.586 ± 0.024	2.416TyrAsp: 2.416 ± 0.052	2.571TyrGlu: 2.571 ± 0.067	1.557TyrPhe: 1.557 ± 0.045	3.065TyrGly: 3.065 ± 0.068	0.94TyrHis: 0.94 ± 0.037	2.31TyrIle: 2.31 ± 0.052	1.791TyrLys: 1.791 ± 0.047	3.647TyrLeu: 3.647 ± 0.077	0.948TyrMet: 0.948 ± 0.033	1.459TyrAsn: 1.459 ± 0.044	1.562TyrPro: 1.562 ± 0.05	1.583TyrGln: 1.583 ± 0.047	2.52TyrArg: 2.52 ± 0.06	2.26TyrSer: 2.26 ± 0.064	2.27TyrThr: 2.27 ± 0.067	2.343TyrVal: 2.343 ± 0.053	0.332TyrTrp: 0.332 ± 0.02	1.681TyrTyr: 1.681 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2601 proteins (830445 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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