Amino acid dipepetide frequency for Pseudocohnilembus persalinus (Ciliate)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.146AlaAla: 1.146 ± 0.016	0.464AlaCys: 0.464 ± 0.013	1.389AlaAsp: 1.389 ± 0.017	1.885AlaGlu: 1.885 ± 0.02	1.492AlaPhe: 1.492 ± 0.016	1.302AlaGly: 1.302 ± 0.02	0.501AlaHis: 0.501 ± 0.009	2.292AlaIle: 2.292 ± 0.023	2.696AlaLys: 2.696 ± 0.024	2.843AlaLeu: 2.843 ± 0.025	0.577AlaMet: 0.577 ± 0.011	2.055AlaAsn: 2.055 ± 0.017	0.75AlaPro: 0.75 ± 0.011	2.486AlaGln: 2.486 ± 0.021	0.908AlaArg: 0.908 ± 0.013	1.851AlaSer: 1.851 ± 0.021	1.186AlaThr: 1.186 ± 0.015	1.363AlaVal: 1.363 ± 0.019	0.206AlaTrp: 0.206 ± 0.006	1.273AlaTyr: 1.273 ± 0.015	0.0AlaXaa: 0.0 ± 0.0
Cys	0.44CysAla: 0.44 ± 0.014	0.228CysCys: 0.228 ± 0.01	0.78CysAsp: 0.78 ± 0.018	0.892CysGlu: 0.892 ± 0.019	0.661CysPhe: 0.661 ± 0.01	0.645CysGly: 0.645 ± 0.015	0.228CysHis: 0.228 ± 0.006	0.998CysIle: 0.998 ± 0.016	1.065CysLys: 1.065 ± 0.018	1.294CysLeu: 1.294 ± 0.025	0.24CysMet: 0.24 ± 0.006	0.808CysAsn: 0.808 ± 0.014	0.516CysPro: 0.516 ± 0.017	1.175CysGln: 1.175 ± 0.022	0.34CysArg: 0.34 ± 0.007	0.962CysSer: 0.962 ± 0.028	0.618CysThr: 0.618 ± 0.019	0.604CysVal: 0.604 ± 0.015	0.093CysTrp: 0.093 ± 0.004	0.617CysTyr: 0.617 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	1.292AspAla: 1.292 ± 0.015	0.697AspCys: 0.697 ± 0.016	3.239AspAsp: 3.239 ± 0.034	4.404AspGlu: 4.404 ± 0.031	3.301AspPhe: 3.301 ± 0.026	1.957AspGly: 1.957 ± 0.027	0.706AspHis: 0.706 ± 0.01	4.71AspIle: 4.71 ± 0.027	4.973AspLys: 4.973 ± 0.033	5.112AspLeu: 5.112 ± 0.029	0.957AspMet: 0.957 ± 0.01	4.182AspAsn: 4.182 ± 0.029	1.489AspPro: 1.489 ± 0.017	4.976AspGln: 4.976 ± 0.039	1.282AspArg: 1.282 ± 0.016	3.468AspSer: 3.468 ± 0.03	1.824AspThr: 1.824 ± 0.021	1.833AspVal: 1.833 ± 0.019	0.378AspTrp: 0.378 ± 0.008	2.502AspTyr: 2.502 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	2.011GluAla: 2.011 ± 0.023	0.813GluCys: 0.813 ± 0.02	4.168GluAsp: 4.168 ± 0.034	7.556GluGlu: 7.556 ± 0.071	3.314GluPhe: 3.314 ± 0.024	2.385GluGly: 2.385 ± 0.027	0.9GluHis: 0.9 ± 0.012	6.724GluIle: 6.724 ± 0.039	9.271GluLys: 9.271 ± 0.066	5.549GluLeu: 5.549 ± 0.037	1.429GluMet: 1.429 ± 0.017	8.565GluAsn: 8.565 ± 0.051	1.072GluPro: 1.072 ± 0.014	7.774GluGln: 7.774 ± 0.059	2.045GluArg: 2.045 ± 0.021	3.74GluSer: 3.74 ± 0.026	2.447GluThr: 2.447 ± 0.02	2.549GluVal: 2.549 ± 0.027	0.416GluTrp: 0.416 ± 0.009	2.77GluTyr: 2.77 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	1.503PheAla: 1.503 ± 0.014	0.725PheCys: 0.725 ± 0.013	2.957PheAsp: 2.957 ± 0.021	3.327PheGlu: 3.327 ± 0.023	2.463PhePhe: 2.463 ± 0.023	2.21PheGly: 2.21 ± 0.023	0.704PheHis: 0.704 ± 0.011	3.491PheIle: 3.491 ± 0.029	4.191PheLys: 4.191 ± 0.029	4.391PheLeu: 4.391 ± 0.031	1.009PheMet: 1.009 ± 0.013	3.901PheAsn: 3.901 ± 0.027	1.23PhePro: 1.23 ± 0.013	4.779PheGln: 4.779 ± 0.026	1.26PheArg: 1.26 ± 0.013	3.725PheSer: 3.725 ± 0.023	1.975PheThr: 1.975 ± 0.018	1.935PheVal: 1.935 ± 0.016	0.376PheTrp: 0.376 ± 0.009	2.37PheTyr: 2.37 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	1.365GlyAla: 1.365 ± 0.018	0.577GlyCys: 0.577 ± 0.012	1.915GlyAsp: 1.915 ± 0.018	2.544GlyGlu: 2.544 ± 0.024	2.018GlyPhe: 2.018 ± 0.022	2.145GlyGly: 2.145 ± 0.039	0.672GlyHis: 0.672 ± 0.01	3.023GlyIle: 3.023 ± 0.023	3.369GlyLys: 3.369 ± 0.029	2.95GlyLeu: 2.95 ± 0.025	0.691GlyMet: 0.691 ± 0.011	2.863GlyAsn: 2.863 ± 0.024	0.776GlyPro: 0.776 ± 0.014	2.909GlyGln: 2.909 ± 0.023	1.025GlyArg: 1.025 ± 0.015	2.423GlySer: 2.423 ± 0.024	1.545GlyThr: 1.545 ± 0.019	1.776GlyVal: 1.776 ± 0.021	0.287GlyTrp: 0.287 ± 0.007	1.809GlyTyr: 1.809 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	0.402HisAla: 0.402 ± 0.008	0.208HisCys: 0.208 ± 0.005	0.638HisAsp: 0.638 ± 0.01	0.864HisGlu: 0.864 ± 0.012	0.859HisPhe: 0.859 ± 0.012	0.559HisGly: 0.559 ± 0.01	0.313HisHis: 0.313 ± 0.008	1.197HisIle: 1.197 ± 0.012	1.335HisLys: 1.335 ± 0.015	1.403HisLeu: 1.403 ± 0.013	0.272HisMet: 0.272 ± 0.007	1.171HisAsn: 1.171 ± 0.013	0.524HisPro: 0.524 ± 0.009	1.38HisGln: 1.38 ± 0.015	0.481HisArg: 0.481 ± 0.008	1.008HisSer: 1.008 ± 0.012	0.562HisThr: 0.562 ± 0.01	0.51HisVal: 0.51 ± 0.009	0.098HisTrp: 0.098 ± 0.004	0.697HisTyr: 0.697 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	2.276IleAla: 2.276 ± 0.02	1.156IleCys: 1.156 ± 0.015	4.526IleAsp: 4.526 ± 0.028	5.723IleGlu: 5.723 ± 0.032	4.01IlePhe: 4.01 ± 0.028	2.69IleGly: 2.69 ± 0.022	1.124IleHis: 1.124 ± 0.013	5.884IleIle: 5.884 ± 0.036	7.741IleLys: 7.741 ± 0.039	6.932IleLeu: 6.932 ± 0.04	1.482IleMet: 1.482 ± 0.015	7.281IleAsn: 7.281 ± 0.04	2.14IlePro: 2.14 ± 0.02	8.467IleGln: 8.467 ± 0.047	2.088IleArg: 2.088 ± 0.017	5.628IleSer: 5.628 ± 0.031	2.917IleThr: 2.917 ± 0.024	2.988IleVal: 2.988 ± 0.028	0.546IleTrp: 0.546 ± 0.011	3.444IleTyr: 3.444 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	2.642LysAla: 2.642 ± 0.025	1.184LysCys: 1.184 ± 0.021	4.825LysAsp: 4.825 ± 0.032	7.844LysGlu: 7.844 ± 0.053	4.229LysPhe: 4.229 ± 0.027	2.984LysGly: 2.984 ± 0.025	1.28LysHis: 1.28 ± 0.015	8.941LysIle: 8.941 ± 0.045	11.678LysLys: 11.678 ± 0.064	8.05LysLeu: 8.05 ± 0.047	2.044LysMet: 2.044 ± 0.019	11.083LysAsn: 11.083 ± 0.065	2.029LysPro: 2.029 ± 0.021	11.148LysGln: 11.148 ± 0.065	2.693LysArg: 2.693 ± 0.023	6.206LysSer: 6.206 ± 0.037	3.666LysThr: 3.666 ± 0.022	3.445LysVal: 3.445 ± 0.024	0.505LysTrp: 0.505 ± 0.009	4.137LysTyr: 4.137 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	2.873LeuAla: 2.873 ± 0.026	1.035LeuCys: 1.035 ± 0.013	4.738LeuAsp: 4.738 ± 0.029	6.961LeuGlu: 6.961 ± 0.043	3.72LeuPhe: 3.72 ± 0.03	3.313LeuGly: 3.313 ± 0.026	1.187LeuHis: 1.187 ± 0.013	6.094LeuIle: 6.094 ± 0.036	9.126LeuLys: 9.126 ± 0.049	6.51LeuLeu: 6.51 ± 0.039	1.581LeuMet: 1.581 ± 0.015	7.805LeuAsn: 7.805 ± 0.036	2.135LeuPro: 2.135 ± 0.019	8.414LeuGln: 8.414 ± 0.046	2.498LeuArg: 2.498 ± 0.022	5.574LeuSer: 5.574 ± 0.028	3.373LeuThr: 3.373 ± 0.024	3.382LeuVal: 3.382 ± 0.024	0.473LeuTrp: 0.473 ± 0.008	3.172LeuTyr: 3.172 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	0.792MetAla: 0.792 ± 0.012	0.215MetCys: 0.215 ± 0.005	1.05MetAsp: 1.05 ± 0.013	1.679MetGlu: 1.679 ± 0.017	0.723MetPhe: 0.723 ± 0.012	0.912MetGly: 0.912 ± 0.012	0.318MetHis: 0.318 ± 0.007	1.371MetIle: 1.371 ± 0.014	1.839MetLys: 1.839 ± 0.017	1.417MetLeu: 1.417 ± 0.016	0.444MetMet: 0.444 ± 0.01	1.542MetAsn: 1.542 ± 0.016	0.513MetPro: 0.513 ± 0.01	1.56MetGln: 1.56 ± 0.017	0.571MetArg: 0.571 ± 0.009	1.287MetSer: 1.287 ± 0.012	0.761MetThr: 0.761 ± 0.012	0.856MetVal: 0.856 ± 0.012	0.11MetTrp: 0.11 ± 0.004	0.651MetTyr: 0.651 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	1.887AsnAla: 1.887 ± 0.018	1.271AsnCys: 1.271 ± 0.026	4.577AsnAsp: 4.577 ± 0.035	6.181AsnGlu: 6.181 ± 0.034	5.005AsnPhe: 5.005 ± 0.033	2.818AsnGly: 2.818 ± 0.026	1.255AsnHis: 1.255 ± 0.014	7.402AsnIle: 7.402 ± 0.04	8.997AsnLys: 8.997 ± 0.053	8.999AsnLeu: 8.999 ± 0.05	1.642AsnMet: 1.642 ± 0.016	11.719AsnAsn: 11.719 ± 0.107	2.33AsnPro: 2.33 ± 0.02	13.029AsnGln: 13.029 ± 0.105	2.093AsnArg: 2.093 ± 0.016	7.292AsnSer: 7.292 ± 0.053	3.389AsnThr: 3.389 ± 0.024	2.681AsnVal: 2.681 ± 0.02	0.507AsnTrp: 0.507 ± 0.01	4.485AsnTyr: 4.485 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	0.727ProAla: 0.727 ± 0.012	0.297ProCys: 0.297 ± 0.01	1.247ProAsp: 1.247 ± 0.016	1.905ProGlu: 1.905 ± 0.017	1.232ProPhe: 1.232 ± 0.016	0.952ProGly: 0.952 ± 0.015	0.403ProHis: 0.403 ± 0.007	1.828ProIle: 1.828 ± 0.018	2.286ProLys: 2.286 ± 0.021	2.053ProLeu: 2.053 ± 0.02	0.371ProMet: 0.371 ± 0.008	2.036ProAsn: 2.036 ± 0.019	0.828ProPro: 0.828 ± 0.016	2.463ProGln: 2.463 ± 0.021	0.657ProArg: 0.657 ± 0.01	1.688ProSer: 1.688 ± 0.016	0.999ProThr: 0.999 ± 0.014	1.196ProVal: 1.196 ± 0.017	0.182ProTrp: 0.182 ± 0.005	1.154ProTyr: 1.154 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	2.473GlnAla: 2.473 ± 0.021	0.951GlnCys: 0.951 ± 0.021	5.086GlnAsp: 5.086 ± 0.045	8.612GlnGlu: 8.612 ± 0.064	4.464GlnPhe: 4.464 ± 0.029	2.99GlnGly: 2.99 ± 0.023	1.313GlnHis: 1.313 ± 0.014	8.88GlnIle: 8.88 ± 0.052	12.14GlnLys: 12.14 ± 0.072	7.602GlnLeu: 7.602 ± 0.043	1.98GlnMet: 1.98 ± 0.02	14.134GlnAsn: 14.134 ± 0.112	1.87GlnPro: 1.87 ± 0.019	16.562GlnGln: 16.562 ± 0.146	2.406GlnArg: 2.406 ± 0.02	6.254GlnSer: 6.254 ± 0.046	3.569GlnThr: 3.569 ± 0.025	3.345GlnVal: 3.345 ± 0.023	0.409GlnTrp: 0.409 ± 0.008	3.882GlnTyr: 3.882 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	0.948ArgAla: 0.948 ± 0.014	0.325ArgCys: 0.325 ± 0.007	1.433ArgAsp: 1.433 ± 0.016	2.095ArgGlu: 2.095 ± 0.022	1.21ArgPhe: 1.21 ± 0.014	1.034ArgGly: 1.034 ± 0.015	0.434ArgHis: 0.434 ± 0.007	2.101ArgIle: 2.101 ± 0.019	2.997ArgLys: 2.997 ± 0.025	2.019ArgLeu: 2.019 ± 0.019	0.507ArgMet: 0.507 ± 0.008	2.261ArgAsn: 2.261 ± 0.021	0.725ArgPro: 0.725 ± 0.011	2.312ArgGln: 2.312 ± 0.019	0.995ArgArg: 0.995 ± 0.014	1.67ArgSer: 1.67 ± 0.019	0.974ArgThr: 0.974 ± 0.013	1.164ArgVal: 1.164 ± 0.013	0.171ArgTrp: 0.171 ± 0.006	1.083ArgTyr: 1.083 ± 0.013	0.0ArgXaa: 0.0 ± 0.0
Ser	1.871SerAla: 1.871 ± 0.021	0.956SerCys: 0.956 ± 0.028	3.615SerAsp: 3.615 ± 0.027	4.587SerGlu: 4.587 ± 0.027	3.264SerPhe: 3.264 ± 0.026	2.571SerGly: 2.571 ± 0.026	1.116SerHis: 1.116 ± 0.015	4.73SerIle: 4.73 ± 0.027	5.898SerLys: 5.898 ± 0.032	5.706SerLeu: 5.706 ± 0.032	1.029SerMet: 1.029 ± 0.013	5.814SerAsn: 5.814 ± 0.045	1.96SerPro: 1.96 ± 0.019	7.921SerGln: 7.921 ± 0.054	1.76SerArg: 1.76 ± 0.016	5.352SerSer: 5.352 ± 0.044	2.575SerThr: 2.575 ± 0.024	2.597SerVal: 2.597 ± 0.022	0.368SerTrp: 0.368 ± 0.009	2.765SerTyr: 2.765 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	1.256ThrAla: 1.256 ± 0.017	0.751ThrCys: 0.751 ± 0.034	1.958ThrAsp: 1.958 ± 0.019	2.456ThrGlu: 2.456 ± 0.019	1.893ThrPhe: 1.893 ± 0.022	1.661ThrGly: 1.661 ± 0.018	0.645ThrHis: 0.645 ± 0.01	2.92ThrIle: 2.92 ± 0.024	3.111ThrLys: 3.111 ± 0.023	3.31ThrLeu: 3.31 ± 0.027	0.605ThrMet: 0.605 ± 0.009	3.235ThrAsn: 3.235 ± 0.024	1.27ThrPro: 1.27 ± 0.016	3.578ThrGln: 3.578 ± 0.03	0.935ThrArg: 0.935 ± 0.011	2.54ThrSer: 2.54 ± 0.023	1.574ThrThr: 1.574 ± 0.018	1.704ThrVal: 1.704 ± 0.019	0.263ThrTrp: 0.263 ± 0.008	1.67ThrTyr: 1.67 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	1.303ValAla: 1.303 ± 0.016	0.596ValCys: 0.596 ± 0.012	2.111ValAsp: 2.111 ± 0.02	2.637ValGlu: 2.637 ± 0.03	2.027ValPhe: 2.027 ± 0.019	1.559ValGly: 1.559 ± 0.018	0.597ValHis: 0.597 ± 0.009	2.837ValIle: 2.837 ± 0.023	3.555ValLys: 3.555 ± 0.028	3.318ValLeu: 3.318 ± 0.023	0.719ValMet: 0.719 ± 0.011	2.844ValAsn: 2.844 ± 0.022	1.121ValPro: 1.121 ± 0.017	3.446ValGln: 3.446 ± 0.025	1.071ValArg: 1.071 ± 0.014	2.519ValSer: 2.519 ± 0.02	1.478ValThr: 1.478 ± 0.019	1.701ValVal: 1.701 ± 0.018	0.304ValTrp: 0.304 ± 0.008	1.756ValTyr: 1.756 ± 0.018	0.0ValXaa: 0.0 ± 0.0
Trp	0.266TrpAla: 0.266 ± 0.006	0.107TrpCys: 0.107 ± 0.007	0.414TrpAsp: 0.414 ± 0.009	0.469TrpGlu: 0.469 ± 0.009	0.269TrpPhe: 0.269 ± 0.006	0.329TrpGly: 0.329 ± 0.009	0.101TrpHis: 0.101 ± 0.004	0.481TrpIle: 0.481 ± 0.011	0.584TrpLys: 0.584 ± 0.01	0.461TrpLeu: 0.461 ± 0.009	0.134TrpMet: 0.134 ± 0.004	0.459TrpAsn: 0.459 ± 0.009	0.155TrpPro: 0.155 ± 0.004	0.343TrpGln: 0.343 ± 0.008	0.206TrpArg: 0.206 ± 0.005	0.394TrpSer: 0.394 ± 0.008	0.26TrpThr: 0.26 ± 0.007	0.341TrpVal: 0.341 ± 0.008	0.06TrpTrp: 0.06 ± 0.003	0.23TrpTyr: 0.23 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.195TyrAla: 1.195 ± 0.015	0.646TyrCys: 0.646 ± 0.013	2.615TyrAsp: 2.615 ± 0.023	2.879TyrGlu: 2.879 ± 0.022	2.37TyrPhe: 2.37 ± 0.021	1.665TyrGly: 1.665 ± 0.018	0.685TyrHis: 0.685 ± 0.01	3.138TyrIle: 3.138 ± 0.029	3.759TyrLys: 3.759 ± 0.025	4.047TyrLeu: 4.047 ± 0.029	0.897TyrMet: 0.897 ± 0.012	3.8TyrAsn: 3.8 ± 0.029	1.071TyrPro: 1.071 ± 0.015	4.202TyrGln: 4.202 ± 0.03	1.145TyrArg: 1.145 ± 0.012	2.872TyrSer: 2.872 ± 0.025	1.642TyrThr: 1.642 ± 0.017	1.531TyrVal: 1.531 ± 0.018	0.31TyrTrp: 0.31 ± 0.006	2.165TyrTyr: 2.165 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 13175 proteins (7254786 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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