Bacillus phage vB_BtS_BMBtp14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skryabinvirinae; Bembunaquatrovirus; Bacillus virus BMBtp14

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P781|A0A1B1P781_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_BMBtp14 OX=1868826 GN=BMBtpLA2_14 PE=4 SV=1
MM1 pKa = 7.81SIEE4 pKa = 3.84AHH6 pKa = 6.07KK7 pKa = 11.26CNVTGCNGLVVFEE20 pKa = 4.74NADD23 pKa = 3.53FDD25 pKa = 4.27LQKK28 pKa = 10.52PDD30 pKa = 3.91TIKK33 pKa = 10.91GVYY36 pKa = 10.37ALDD39 pKa = 3.84NPACNVCGKK48 pKa = 9.77EE49 pKa = 3.86FLVVPSYY56 pKa = 11.52SVIDD60 pKa = 3.95FDD62 pKa = 5.42EE63 pKa = 4.49DD64 pKa = 3.6TQEE67 pKa = 4.11FEE69 pKa = 4.86EE70 pKa = 5.09IEE72 pKa = 4.16PACITEE78 pKa = 4.04WQKK81 pKa = 11.45QKK83 pKa = 10.72II84 pKa = 3.96

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P7B7|A0A1B1P7B7_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_BMBtp14 OX=1868826 GN=BMBtpLA2_28 PE=4 SV=1
MM1 pKa = 7.24NRR3 pKa = 11.84LITVVTKK10 pKa = 10.92GIGSIRR16 pKa = 11.84KK17 pKa = 9.48AIVNYY22 pKa = 8.79MSKK25 pKa = 10.43PNEE28 pKa = 3.96VQVAASAVTVGGTDD42 pKa = 3.48EE43 pKa = 4.28EE44 pKa = 4.52KK45 pKa = 10.58KK46 pKa = 10.4IPGRR50 pKa = 11.84ISWFTDD56 pKa = 3.11HH57 pKa = 7.18QIEE60 pKa = 4.38EE61 pKa = 4.36SDD63 pKa = 3.71KK64 pKa = 11.42GPINIQTQAHH74 pKa = 6.32IDD76 pKa = 3.72GVKK79 pKa = 10.32LFRR82 pKa = 11.84SFLTSARR89 pKa = 11.84KK90 pKa = 9.37QSINRR95 pKa = 11.84QLSYY99 pKa = 11.11NKK101 pKa = 10.3KK102 pKa = 8.14KK103 pKa = 7.0TQRR106 pKa = 11.84RR107 pKa = 11.84NWSKK111 pKa = 8.92WKK113 pKa = 10.02RR114 pKa = 11.84RR115 pKa = 11.84NRR117 pKa = 3.5

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15145

39

1975

201.9

22.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.494 ± 0.777

0.799 ± 0.135

5.685 ± 0.201

8.161 ± 0.384

3.711 ± 0.159

6.121 ± 0.379

1.638 ± 0.139

7.164 ± 0.281

8.775 ± 0.315

7.844 ± 0.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.195

5.229 ± 0.225

3.004 ± 0.256

4.318 ± 0.244

4.761 ± 0.219

5.883 ± 0.248

5.672 ± 0.276

6.504 ± 0.233

1.162 ± 0.114

3.301 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski