Clostridiales bacterium COT073_COT-073

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P2AL81|A0A3P2AL81_9FIRM ATP-dependent sacrificial sulfur transferase LarE OS=Clostridiales bacterium COT073_COT-073 OX=2491044 GN=larE PE=4 SV=1
MM1 pKa = 7.22LTLDD5 pKa = 3.5EE6 pKa = 4.7FFRR9 pKa = 11.84DD10 pKa = 3.61NVVEE14 pKa = 5.49DD15 pKa = 4.85SIAPNQWLYY24 pKa = 11.28GRR26 pKa = 11.84PSILDD31 pKa = 2.81IWGMLNEE38 pKa = 4.54LEE40 pKa = 4.52KK41 pKa = 10.97SNKK44 pKa = 8.26IAWVRR49 pKa = 11.84VSLHH53 pKa = 7.49DD54 pKa = 3.73DD55 pKa = 3.61TEE57 pKa = 4.95IEE59 pKa = 3.91VDD61 pKa = 3.85DD62 pKa = 4.77NGEE65 pKa = 4.06EE66 pKa = 4.15EE67 pKa = 5.35LFLLGDD73 pKa = 4.23TIVICTSMNEE83 pKa = 5.45DD84 pKa = 3.54EE85 pKa = 4.96LQDD88 pKa = 4.38NINTQWLCADD98 pKa = 3.83GVIPAEE104 pKa = 3.92MEE106 pKa = 4.54GYY108 pKa = 10.91SNIPQIPEE116 pKa = 3.53QCSCYY121 pKa = 10.3EE122 pKa = 4.2IVWDD126 pKa = 4.13

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P2AH32|A0A3P2AH32_9FIRM Uncharacterized protein OS=Clostridiales bacterium COT073_COT-073 OX=2491044 GN=EII17_05625 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 10.63VEE4 pKa = 4.04VQSPYY9 pKa = 10.84NRR11 pKa = 11.84ITISYY16 pKa = 8.19NRR18 pKa = 11.84KK19 pKa = 6.28GHH21 pKa = 6.12RR22 pKa = 11.84AFAVFLLSIFVGKK35 pKa = 9.85CRR37 pKa = 11.84RR38 pKa = 11.84AVSDD42 pKa = 3.5MRR44 pKa = 11.84CRR46 pKa = 11.84SPPLHH51 pKa = 7.2LDD53 pKa = 3.35FLHH56 pKa = 6.84FKK58 pKa = 10.75NSDD61 pKa = 2.88GRR63 pKa = 11.84YY64 pKa = 8.71LWW66 pKa = 3.74

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2881

0

2881

986005

17

3658

342.2

38.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.318 ± 0.049

1.018 ± 0.019

5.107 ± 0.034

7.592 ± 0.046

4.463 ± 0.039

6.572 ± 0.042

1.721 ± 0.019

8.072 ± 0.046

7.416 ± 0.043

9.63 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.024

4.661 ± 0.035

3.373 ± 0.026

3.946 ± 0.036

4.06 ± 0.032

5.73 ± 0.031

4.95 ± 0.04

6.229 ± 0.034

1.021 ± 0.017

4.222 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski