Proteome-pI 2.0 - Proteome Isoelectric Point Database

Streptococcus phage Javan580

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A4D6BB12|A0A4D6BB12_9CAUD Uncharacterized protein OS=Streptococcus phage Javan580 OX=2548272 GN=Javan580_0021 PE=4 SV=1

MM1 pKa = 8.01IEE3 pKa = 4.23VITLNFLTEE12 pKa = 4.12HH13 pKa = 6.72LSVPVYY19 pKa = 9.97TEE21 pKa = 4.37HH22 pKa = 6.95EE23 pKa = 4.31EE24 pKa = 4.05EE25 pKa = 4.38MPDD28 pKa = 3.33SFVVFEE34 pKa = 4.48KK35 pKa = 10.54TSGGKK40 pKa = 9.62KK41 pKa = 9.16NHH43 pKa = 6.6LNQATLAIQSYY54 pKa = 8.75GQSLEE59 pKa = 3.77EE60 pKa = 3.49AAFLNEE66 pKa = 4.0EE67 pKa = 4.09VKK69 pKa = 10.64QAVEE73 pKa = 4.28KK74 pKa = 10.3MVEE77 pKa = 4.05LPSISRR83 pKa = 11.84VEE85 pKa = 4.16LNSDD89 pKa = 3.77YY90 pKa = 11.4NFTDD94 pKa = 3.57TEE96 pKa = 4.47TKK98 pKa = 9.97RR99 pKa = 11.84YY100 pKa = 9.56RR101 pKa = 11.84YY102 pKa = 9.11QAVVDD107 pKa = 5.34FIYY110 pKa = 10.56FF111 pKa = 3.62

Molecular weight: 12.86 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A4D6BCJ6|A0A4D6BCJ6_9CAUD Endolysin OS=Streptococcus phage Javan580 OX=2548272 GN=Javan580_0020 PE=4 SV=1

MM1 pKa = 7.49ARR3 pKa = 11.84MKK5 pKa = 10.7FKK7 pKa = 11.02LNRR10 pKa = 11.84AGVRR14 pKa = 11.84DD15 pKa = 3.89LLKK18 pKa = 10.65SPEE21 pKa = 4.05MQAVLTDD28 pKa = 3.37KK29 pKa = 11.39ANAIRR34 pKa = 11.84NRR36 pKa = 11.84AGDD39 pKa = 4.0GYY41 pKa = 11.09EE42 pKa = 3.67SDD44 pKa = 3.49IYY46 pKa = 10.89VGKK49 pKa = 8.48TRR51 pKa = 11.84ANAMVYY57 pKa = 10.69ADD59 pKa = 4.22SIKK62 pKa = 10.85AKK64 pKa = 10.25RR65 pKa = 11.84DD66 pKa = 3.58NKK68 pKa = 10.31KK69 pKa = 10.32HH70 pKa = 4.82NTLLKK75 pKa = 10.33AVKK78 pKa = 10.03SS79 pKa = 3.89

Molecular weight: 8.85 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
24	0	24	6349	38	1768	264.5	29.19

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.411 ± 0.668	0.473 ± 0.144	5.371 ± 0.279	6.725 ± 0.701	3.497 ± 0.276	7.308 ± 0.886	1.418 ± 0.278	6.851 ± 0.349	6.757 ± 0.485	8.332 ± 0.512

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.252 ± 0.394	5.056 ± 0.234	2.63 ± 0.373	3.985 ± 0.335	4.473 ± 0.545	7.607 ± 0.685	7.639 ± 0.791	6.836 ± 0.369	0.929 ± 0.168	3.449 ± 0.344

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski