Mycobacterium sp. THAF192

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5641 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8ZZS6|A0A5P8ZZS6_9MYCO Subtilisin DY OS=Mycobacterium sp. THAF192 OX=2587868 GN=apr2 PE=4 SV=1
MM1 pKa = 7.15ATSTHH6 pKa = 6.37RR7 pKa = 11.84AALLTCASALLLTACSFSFSSGGLDD32 pKa = 4.09HH33 pKa = 7.41EE34 pKa = 4.76KK35 pKa = 10.46LQNAITDD42 pKa = 3.97EE43 pKa = 4.69LNTSYY48 pKa = 9.49EE49 pKa = 4.34TISQQVSSVDD59 pKa = 3.63CPQDD63 pKa = 3.46QNPGPGEE70 pKa = 4.54KK71 pKa = 9.24ILCTAQVGDD80 pKa = 3.24QSVRR84 pKa = 11.84VEE86 pKa = 4.16STVTDD91 pKa = 3.02EE92 pKa = 4.78DD93 pKa = 5.17YY94 pKa = 11.5NVNFEE99 pKa = 4.5TLDD102 pKa = 3.45TLYY105 pKa = 10.8DD106 pKa = 3.91LPSVGTTLSDD116 pKa = 3.31EE117 pKa = 4.51LTNQLGFPVTVTCGEE132 pKa = 3.98GLKK135 pKa = 10.53AVEE138 pKa = 4.0IGQTFDD144 pKa = 3.29CTAADD149 pKa = 3.61EE150 pKa = 5.39DD151 pKa = 4.38GDD153 pKa = 3.9EE154 pKa = 4.15RR155 pKa = 11.84TVRR158 pKa = 11.84LTAAPIGEE166 pKa = 3.99NDD168 pKa = 3.03QWEE171 pKa = 4.33LLEE174 pKa = 4.23

Molecular weight: 18.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8ZKX8|A0A5P8ZKX8_9MYCO 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Mycobacterium sp. THAF192 OX=2587868 GN=menA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5641

0

5641

1818666

30

3682

322.4

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.217 ± 0.046

0.795 ± 0.009

6.59 ± 0.027

5.315 ± 0.031

2.998 ± 0.018

8.83 ± 0.03

2.189 ± 0.016

4.061 ± 0.02

1.975 ± 0.022

9.737 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.016

2.115 ± 0.015

5.836 ± 0.03

3.001 ± 0.016

7.3 ± 0.031

5.422 ± 0.022

6.171 ± 0.026

8.797 ± 0.031

1.513 ± 0.012

2.074 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski