Amino acid dipepetide frequency for Synechococcus sp. (strain JA-2-3B a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.168AlaAla: 11.168 ± 0.156	1.072AlaCys: 1.072 ± 0.034	4.67AlaAsp: 4.67 ± 0.077	7.007AlaGlu: 7.007 ± 0.104	3.244AlaPhe: 3.244 ± 0.064	7.807AlaGly: 7.807 ± 0.106	2.001AlaHis: 2.001 ± 0.053	5.013AlaIle: 5.013 ± 0.085	2.961AlaLys: 2.961 ± 0.079	12.298AlaLeu: 12.298 ± 0.143	1.873AlaMet: 1.873 ± 0.056	2.425AlaAsn: 2.425 ± 0.068	4.091AlaPro: 4.091 ± 0.084	6.233AlaGln: 6.233 ± 0.1	6.106AlaArg: 6.106 ± 0.097	4.612AlaSer: 4.612 ± 0.082	4.393AlaThr: 4.393 ± 0.079	6.968AlaVal: 6.968 ± 0.098	1.618AlaTrp: 1.618 ± 0.049	2.611AlaTyr: 2.611 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.736CysAla: 0.736 ± 0.031	0.201CysCys: 0.201 ± 0.017	0.464CysAsp: 0.464 ± 0.026	0.488CysGlu: 0.488 ± 0.024	0.41CysPhe: 0.41 ± 0.026	1.031CysGly: 1.031 ± 0.039	0.33CysHis: 0.33 ± 0.019	0.467CysIle: 0.467 ± 0.023	0.276CysLys: 0.276 ± 0.017	1.271CysLeu: 1.271 ± 0.038	0.148CysMet: 0.148 ± 0.015	0.284CysAsn: 0.284 ± 0.017	0.757CysPro: 0.757 ± 0.037	0.601CysGln: 0.601 ± 0.024	0.979CysArg: 0.979 ± 0.034	0.721CysSer: 0.721 ± 0.028	0.489CysThr: 0.489 ± 0.023	0.635CysVal: 0.635 ± 0.03	0.217CysTrp: 0.217 ± 0.015	0.344CysTyr: 0.344 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	3.348AspAla: 3.348 ± 0.063	0.507AspCys: 0.507 ± 0.028	1.24AspAsp: 1.24 ± 0.038	2.373AspGlu: 2.373 ± 0.064	1.795AspPhe: 1.795 ± 0.049	3.17AspGly: 3.17 ± 0.06	0.907AspHis: 0.907 ± 0.034	2.04AspIle: 2.04 ± 0.055	1.057AspLys: 1.057 ± 0.039	6.252AspLeu: 6.252 ± 0.1	0.685AspMet: 0.685 ± 0.03	1.008AspAsn: 1.008 ± 0.033	5.429AspPro: 5.429 ± 0.085	1.847AspGln: 1.847 ± 0.048	3.277AspArg: 3.277 ± 0.069	2.036AspSer: 2.036 ± 0.05	1.682AspThr: 1.682 ± 0.045	2.517AspVal: 2.517 ± 0.047	1.068AspTrp: 1.068 ± 0.041	1.458AspTyr: 1.458 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	6.944GluAla: 6.944 ± 0.103	0.458GluCys: 0.458 ± 0.022	2.603GluAsp: 2.603 ± 0.065	4.361GluGlu: 4.361 ± 0.102	1.922GluPhe: 1.922 ± 0.048	4.452GluGly: 4.452 ± 0.081	1.213GluHis: 1.213 ± 0.041	3.311GluIle: 3.311 ± 0.072	2.379GluLys: 2.379 ± 0.051	7.341GluLeu: 7.341 ± 0.108	1.333GluMet: 1.333 ± 0.041	1.473GluAsn: 1.473 ± 0.043	2.977GluPro: 2.977 ± 0.071	3.907GluGln: 3.907 ± 0.087	5.582GluArg: 5.582 ± 0.096	2.701GluSer: 2.701 ± 0.056	2.847GluThr: 2.847 ± 0.065	4.596GluVal: 4.596 ± 0.08	0.995GluTrp: 0.995 ± 0.036	1.37GluTyr: 1.37 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.284PheAla: 3.284 ± 0.067	0.555PheCys: 0.555 ± 0.024	1.802PheAsp: 1.802 ± 0.043	1.83PheGlu: 1.83 ± 0.042	1.473PhePhe: 1.473 ± 0.048	2.959PheGly: 2.959 ± 0.068	0.658PheHis: 0.658 ± 0.027	1.366PheIle: 1.366 ± 0.044	0.951PheLys: 0.951 ± 0.033	3.823PheLeu: 3.823 ± 0.076	0.516PheMet: 0.516 ± 0.025	1.045PheAsn: 1.045 ± 0.039	2.001PhePro: 2.001 ± 0.051	1.685PheGln: 1.685 ± 0.041	2.305PheArg: 2.305 ± 0.059	2.201PheSer: 2.201 ± 0.055	1.662PheThr: 1.662 ± 0.046	2.336PheVal: 2.336 ± 0.058	0.818PheTrp: 0.818 ± 0.036	1.218PheTyr: 1.218 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.771GlyAla: 5.771 ± 0.11	0.974GlyCys: 0.974 ± 0.033	3.431GlyAsp: 3.431 ± 0.062	4.448GlyGlu: 4.448 ± 0.073	2.939GlyPhe: 2.939 ± 0.06	5.498GlyGly: 5.498 ± 0.098	1.526GlyHis: 1.526 ± 0.049	5.885GlyIle: 5.885 ± 0.09	2.824GlyLys: 2.824 ± 0.062	9.155GlyLeu: 9.155 ± 0.143	1.697GlyMet: 1.697 ± 0.045	1.959GlyAsn: 1.959 ± 0.06	2.796GlyPro: 2.796 ± 0.058	4.075GlyGln: 4.075 ± 0.073	5.147GlyArg: 5.147 ± 0.067	6.92GlySer: 6.92 ± 0.099	3.306GlyThr: 3.306 ± 0.068	5.163GlyVal: 5.163 ± 0.082	1.799GlyTrp: 1.799 ± 0.049	2.351GlyTyr: 2.351 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	1.326HisAla: 1.326 ± 0.043	0.381HisCys: 0.381 ± 0.02	0.637HisAsp: 0.637 ± 0.027	0.779HisGlu: 0.779 ± 0.035	0.894HisPhe: 0.894 ± 0.03	1.317HisGly: 1.317 ± 0.043	0.692HisHis: 0.692 ± 0.033	0.984HisIle: 0.984 ± 0.038	0.507HisLys: 0.507 ± 0.025	3.189HisLeu: 3.189 ± 0.067	0.251HisMet: 0.251 ± 0.022	0.527HisAsn: 0.527 ± 0.026	1.787HisPro: 1.787 ± 0.049	1.032HisGln: 1.032 ± 0.035	1.673HisArg: 1.673 ± 0.045	1.14HisSer: 1.14 ± 0.036	0.953HisThr: 0.953 ± 0.033	0.954HisVal: 0.954 ± 0.032	0.492HisTrp: 0.492 ± 0.023	0.675HisTyr: 0.675 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	4.924IleAla: 4.924 ± 0.08	0.612IleCys: 0.612 ± 0.023	2.247IleAsp: 2.247 ± 0.059	2.788IleGlu: 2.788 ± 0.066	1.6IlePhe: 1.6 ± 0.047	3.805IleGly: 3.805 ± 0.08	1.213IleHis: 1.213 ± 0.037	1.499IleIle: 1.499 ± 0.05	1.067IleLys: 1.067 ± 0.044	6.024IleLeu: 6.024 ± 0.09	0.472IleMet: 0.472 ± 0.025	1.227IleAsn: 1.227 ± 0.041	4.343IlePro: 4.343 ± 0.072	2.726IleGln: 2.726 ± 0.072	3.819IleArg: 3.819 ± 0.066	2.591IleSer: 2.591 ± 0.059	2.323IleThr: 2.323 ± 0.057	2.911IleVal: 2.911 ± 0.063	0.709IleTrp: 0.709 ± 0.026	1.177IleTyr: 1.177 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	3.361LysAla: 3.361 ± 0.074	0.272LysCys: 0.272 ± 0.019	1.267LysAsp: 1.267 ± 0.041	1.667LysGlu: 1.667 ± 0.054	0.67LysPhe: 0.67 ± 0.029	2.373LysGly: 2.373 ± 0.053	0.48LysHis: 0.48 ± 0.025	1.324LysIle: 1.324 ± 0.049	1.02LysLys: 1.02 ± 0.04	3.619LysLeu: 3.619 ± 0.077	0.539LysMet: 0.539 ± 0.025	0.717LysAsn: 0.717 ± 0.032	1.768LysPro: 1.768 ± 0.048	1.499LysGln: 1.499 ± 0.043	2.045LysArg: 2.045 ± 0.049	1.569LysSer: 1.569 ± 0.048	1.656LysThr: 1.656 ± 0.051	2.282LysVal: 2.282 ± 0.06	0.296LysTrp: 0.296 ± 0.019	0.644LysTyr: 0.644 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	13.484LeuAla: 13.484 ± 0.159	1.222LeuCys: 1.222 ± 0.043	5.543LeuAsp: 5.543 ± 0.091	8.19LeuGlu: 8.19 ± 0.116	3.694LeuPhe: 3.694 ± 0.08	9.957LeuGly: 9.957 ± 0.143	2.359LeuHis: 2.359 ± 0.057	5.315LeuIle: 5.315 ± 0.091	3.857LeuLys: 3.857 ± 0.075	14.448LeuLeu: 14.448 ± 0.211	2.117LeuMet: 2.117 ± 0.05	3.42LeuAsn: 3.42 ± 0.063	7.402LeuPro: 7.402 ± 0.117	7.492LeuGln: 7.492 ± 0.124	8.695LeuArg: 8.695 ± 0.11	7.667LeuSer: 7.667 ± 0.098	6.152LeuThr: 6.152 ± 0.085	8.052LeuVal: 8.052 ± 0.109	2.055LeuTrp: 2.055 ± 0.062	2.758LeuTyr: 2.758 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.202MetAla: 2.202 ± 0.061	0.115MetCys: 0.115 ± 0.014	0.787MetAsp: 0.787 ± 0.031	1.009MetGlu: 1.009 ± 0.033	0.426MetPhe: 0.426 ± 0.026	1.624MetGly: 1.624 ± 0.046	0.267MetHis: 0.267 ± 0.018	0.722MetIle: 0.722 ± 0.03	0.529MetLys: 0.529 ± 0.025	1.821MetLeu: 1.821 ± 0.052	0.307MetMet: 0.307 ± 0.02	0.464MetAsn: 0.464 ± 0.022	1.046MetPro: 1.046 ± 0.031	0.901MetGln: 0.901 ± 0.034	1.211MetArg: 1.211 ± 0.04	1.104MetSer: 1.104 ± 0.036	1.053MetThr: 1.053 ± 0.039	1.365MetVal: 1.365 ± 0.044	0.158MetTrp: 0.158 ± 0.014	0.25MetTyr: 0.25 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.261AsnAla: 2.261 ± 0.056	0.324AsnCys: 0.324 ± 0.016	0.774AsnAsp: 0.774 ± 0.031	0.911AsnGlu: 0.911 ± 0.038	1.015AsnPhe: 1.015 ± 0.04	1.938AsnGly: 1.938 ± 0.054	0.577AsnHis: 0.577 ± 0.025	1.134AsnIle: 1.134 ± 0.032	0.613AsnLys: 0.613 ± 0.03	3.467AsnLeu: 3.467 ± 0.079	0.36AsnMet: 0.36 ± 0.018	0.637AsnAsn: 0.637 ± 0.034	2.389AsnPro: 2.389 ± 0.058	1.309AsnGln: 1.309 ± 0.044	2.16AsnArg: 2.16 ± 0.053	1.426AsnSer: 1.426 ± 0.047	1.264AsnThr: 1.264 ± 0.045	1.411AsnVal: 1.411 ± 0.045	0.632AsnTrp: 0.632 ± 0.027	0.729AsnTyr: 0.729 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	5.752ProAla: 5.752 ± 0.089	0.542ProCys: 0.542 ± 0.023	3.27ProAsp: 3.27 ± 0.063	5.516ProGlu: 5.516 ± 0.083	2.153ProPhe: 2.153 ± 0.046	4.434ProGly: 4.434 ± 0.079	1.316ProHis: 1.316 ± 0.038	2.676ProIle: 2.676 ± 0.063	1.758ProLys: 1.758 ± 0.047	7.141ProLeu: 7.141 ± 0.107	1.011ProMet: 1.011 ± 0.035	1.667ProAsn: 1.667 ± 0.056	4.002ProPro: 4.002 ± 0.093	3.711ProGln: 3.711 ± 0.076	3.269ProArg: 3.269 ± 0.063	3.674ProSer: 3.674 ± 0.084	3.327ProThr: 3.327 ± 0.093	4.3ProVal: 4.3 ± 0.066	1.021ProTrp: 1.021 ± 0.037	1.51ProTyr: 1.51 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	6.967GlnAla: 6.967 ± 0.12	0.289GlnCys: 0.289 ± 0.019	2.051GlnAsp: 2.051 ± 0.05	3.998GlnGlu: 3.998 ± 0.086	1.624GlnPhe: 1.624 ± 0.044	4.453GlnGly: 4.453 ± 0.084	1.027GlnHis: 1.027 ± 0.037	3.009GlnIle: 3.009 ± 0.071	1.786GlnLys: 1.786 ± 0.047	6.523GlnLeu: 6.523 ± 0.101	1.125GlnMet: 1.125 ± 0.038	1.256GlnAsn: 1.256 ± 0.038	3.518GlnPro: 3.518 ± 0.071	3.928GlnGln: 3.928 ± 0.114	4.371GlnArg: 4.371 ± 0.074	2.447GlnSer: 2.447 ± 0.057	2.612GlnThr: 2.612 ± 0.06	5.17GlnVal: 5.17 ± 0.079	0.753GlnTrp: 0.753 ± 0.033	0.946GlnTyr: 0.946 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	6.104ArgAla: 6.104 ± 0.09	0.863ArgCys: 0.863 ± 0.035	3.354ArgAsp: 3.354 ± 0.068	4.514ArgGlu: 4.514 ± 0.084	2.753ArgPhe: 2.753 ± 0.061	4.545ArgGly: 4.545 ± 0.084	1.415ArgHis: 1.415 ± 0.048	3.767ArgIle: 3.767 ± 0.069	2.019ArgLys: 2.019 ± 0.052	9.192ArgLeu: 9.192 ± 0.121	1.239ArgMet: 1.239 ± 0.038	1.553ArgAsn: 1.553 ± 0.044	3.692ArgPro: 3.692 ± 0.069	5.201ArgGln: 5.201 ± 0.08	5.743ArgArg: 5.743 ± 0.098	4.106ArgSer: 4.106 ± 0.074	2.801ArgThr: 2.801 ± 0.056	4.962ArgVal: 4.962 ± 0.089	1.573ArgTrp: 1.573 ± 0.053	2.295ArgTyr: 2.295 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.929SerAla: 4.929 ± 0.09	0.636SerCys: 0.636 ± 0.025	2.124SerAsp: 2.124 ± 0.051	2.974SerGlu: 2.974 ± 0.056	2.221SerPhe: 2.221 ± 0.056	4.939SerGly: 4.939 ± 0.088	1.211SerHis: 1.211 ± 0.036	2.208SerIle: 2.208 ± 0.059	1.545SerLys: 1.545 ± 0.052	7.964SerLeu: 7.964 ± 0.11	1.073SerMet: 1.073 ± 0.039	1.446SerAsn: 1.446 ± 0.042	4.454SerPro: 4.454 ± 0.094	3.137SerGln: 3.137 ± 0.061	4.411SerArg: 4.411 ± 0.08	3.788SerSer: 3.788 ± 0.073	2.589SerThr: 2.589 ± 0.057	3.29SerVal: 3.29 ± 0.069	1.039SerTrp: 1.039 ± 0.032	1.344SerTyr: 1.344 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.691ThrAla: 4.691 ± 0.079	0.557ThrCys: 0.557 ± 0.026	1.967ThrAsp: 1.967 ± 0.043	2.529ThrGlu: 2.529 ± 0.05	1.733ThrPhe: 1.733 ± 0.045	4.04ThrGly: 4.04 ± 0.075	0.922ThrHis: 0.922 ± 0.03	1.805ThrIle: 1.805 ± 0.049	0.866ThrLys: 0.866 ± 0.035	6.6ThrLeu: 6.6 ± 0.095	0.601ThrMet: 0.601 ± 0.03	0.971ThrAsn: 0.971 ± 0.036	3.664ThrPro: 3.664 ± 0.113	2.258ThrGln: 2.258 ± 0.063	2.672ThrArg: 2.672 ± 0.053	2.52ThrSer: 2.52 ± 0.057	2.321ThrThr: 2.321 ± 0.058	3.521ThrVal: 3.521 ± 0.068	0.813ThrTrp: 0.813 ± 0.032	1.446ThrTyr: 1.446 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	7.273ValAla: 7.273 ± 0.096	0.756ValCys: 0.756 ± 0.027	3.351ValAsp: 3.351 ± 0.067	4.879ValGlu: 4.879 ± 0.079	2.346ValPhe: 2.346 ± 0.055	5.737ValGly: 5.737 ± 0.096	1.152ValHis: 1.152 ± 0.037	3.58ValIle: 3.58 ± 0.073	2.019ValLys: 2.019 ± 0.052	7.946ValLeu: 7.946 ± 0.108	1.361ValMet: 1.361 ± 0.046	2.112ValAsn: 2.112 ± 0.065	3.351ValPro: 3.351 ± 0.072	3.41ValGln: 3.41 ± 0.067	4.461ValArg: 4.461 ± 0.071	3.828ValSer: 3.828 ± 0.072	3.189ValThr: 3.189 ± 0.068	5.698ValVal: 5.698 ± 0.086	1.053ValTrp: 1.053 ± 0.034	1.682ValTyr: 1.682 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	1.552TrpAla: 1.552 ± 0.047	0.143TrpCys: 0.143 ± 0.013	0.888TrpAsp: 0.888 ± 0.033	1.027TrpGlu: 1.027 ± 0.033	0.565TrpPhe: 0.565 ± 0.025	1.453TrpGly: 1.453 ± 0.045	0.321TrpHis: 0.321 ± 0.018	0.981TrpIle: 0.981 ± 0.033	0.441TrpLys: 0.441 ± 0.024	2.495TrpLeu: 2.495 ± 0.064	0.343TrpMet: 0.343 ± 0.023	0.47TrpAsn: 0.47 ± 0.023	0.934TrpPro: 0.934 ± 0.039	1.5TrpGln: 1.5 ± 0.044	1.215TrpArg: 1.215 ± 0.043	0.773TrpSer: 0.773 ± 0.031	0.617TrpThr: 0.617 ± 0.027	1.559TrpVal: 1.559 ± 0.042	0.307TrpTrp: 0.307 ± 0.018	0.322TrpTyr: 0.322 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.093TyrAla: 2.093 ± 0.053	0.371TyrCys: 0.371 ± 0.023	1.227TyrAsp: 1.227 ± 0.04	1.474TyrGlu: 1.474 ± 0.046	1.013TyrPhe: 1.013 ± 0.037	2.133TyrGly: 2.133 ± 0.05	0.606TyrHis: 0.606 ± 0.032	0.991TyrIle: 0.991 ± 0.034	0.595TyrLys: 0.595 ± 0.026	3.312TyrLeu: 3.312 ± 0.067	0.307TyrMet: 0.307 ± 0.02	0.672TyrAsn: 0.672 ± 0.028	1.65TyrPro: 1.65 ± 0.047	1.403TyrGln: 1.403 ± 0.045	2.551TyrArg: 2.551 ± 0.057	1.495TyrSer: 1.495 ± 0.042	1.259TyrThr: 1.259 ± 0.041	1.494TyrVal: 1.494 ± 0.044	0.486TyrTrp: 0.486 ± 0.026	0.822TyrTyr: 0.822 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2835 proteins (860160 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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