Amino acid dipepetide frequency for Firmicutes bacterium CAG:272

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.981AlaAla: 9.981 ± 0.193	1.242AlaCys: 1.242 ± 0.049	6.018AlaAsp: 6.018 ± 0.1	7.001AlaGlu: 7.001 ± 0.112	3.386AlaPhe: 3.386 ± 0.071	7.11AlaGly: 7.11 ± 0.122	1.208AlaHis: 1.208 ± 0.043	4.375AlaIle: 4.375 ± 0.08	4.768AlaLys: 4.768 ± 0.099	7.529AlaLeu: 7.529 ± 0.122	2.094AlaMet: 2.094 ± 0.065	2.317AlaAsn: 2.317 ± 0.064	2.692AlaPro: 2.692 ± 0.072	1.704AlaGln: 1.704 ± 0.054	3.276AlaArg: 3.276 ± 0.071	4.468AlaSer: 4.468 ± 0.092	3.111AlaThr: 3.111 ± 0.069	8.754AlaVal: 8.754 ± 0.148	0.554AlaTrp: 0.554 ± 0.032	2.862AlaTyr: 2.862 ± 0.072	0.003AlaXaa: 0.003 ± 0.002
Cys	1.555CysAla: 1.555 ± 0.053	0.312CysCys: 0.312 ± 0.023	1.272CysAsp: 1.272 ± 0.051	1.019CysGlu: 1.019 ± 0.042	0.792CysPhe: 0.792 ± 0.035	2.213CysGly: 2.213 ± 0.067	0.34CysHis: 0.34 ± 0.025	0.951CysIle: 0.951 ± 0.033	0.584CysLys: 0.584 ± 0.032	1.384CysLeu: 1.384 ± 0.047	0.361CysMet: 0.361 ± 0.02	0.509CysAsn: 0.509 ± 0.03	0.816CysPro: 0.816 ± 0.04	0.291CysGln: 0.291 ± 0.019	1.472CysArg: 1.472 ± 0.054	1.068CysSer: 1.068 ± 0.039	0.895CysThr: 0.895 ± 0.036	1.142CysVal: 1.142 ± 0.042	0.113CysTrp: 0.113 ± 0.011	0.581CysTyr: 0.581 ± 0.036	0.0CysXaa: 0.0 ± 0.0
Asp	4.845AspAla: 4.845 ± 0.094	1.082AspCys: 1.082 ± 0.042	3.613AspAsp: 3.613 ± 0.089	4.339AspGlu: 4.339 ± 0.083	2.925AspPhe: 2.925 ± 0.061	6.669AspGly: 6.669 ± 0.124	0.823AspHis: 0.823 ± 0.038	5.007AspIle: 5.007 ± 0.084	2.905AspLys: 2.905 ± 0.08	4.241AspLeu: 4.241 ± 0.079	1.711AspMet: 1.711 ± 0.051	2.366AspAsn: 2.366 ± 0.055	2.426AspPro: 2.426 ± 0.069	0.774AspGln: 0.774 ± 0.037	4.116AspArg: 4.116 ± 0.074	3.98AspSer: 3.98 ± 0.079	3.553AspThr: 3.553 ± 0.08	3.853AspVal: 3.853 ± 0.082	0.59AspTrp: 0.59 ± 0.032	2.755AspTyr: 2.755 ± 0.073	0.0AspXaa: 0.0 ± 0.0
Glu	5.25GluAla: 5.25 ± 0.096	0.972GluCys: 0.972 ± 0.042	3.255GluAsp: 3.255 ± 0.081	4.547GluGlu: 4.547 ± 0.092	2.461GluPhe: 2.461 ± 0.061	4.02GluGly: 4.02 ± 0.079	1.124GluHis: 1.124 ± 0.047	4.69GluIle: 4.69 ± 0.094	5.887GluLys: 5.887 ± 0.11	5.796GluLeu: 5.796 ± 0.101	1.974GluMet: 1.974 ± 0.052	4.019GluAsn: 4.019 ± 0.081	2.141GluPro: 2.141 ± 0.062	1.716GluGln: 1.716 ± 0.059	3.557GluArg: 3.557 ± 0.078	3.545GluSer: 3.545 ± 0.077	3.711GluThr: 3.711 ± 0.078	3.503GluVal: 3.503 ± 0.084	0.659GluTrp: 0.659 ± 0.038	3.279GluTyr: 3.279 ± 0.079	0.0GluXaa: 0.0 ± 0.0
Phe	3.67PheAla: 3.67 ± 0.07	0.967PheCys: 0.967 ± 0.041	3.11PheAsp: 3.11 ± 0.072	2.453PheGlu: 2.453 ± 0.056	1.989PhePhe: 1.989 ± 0.058	3.559PheGly: 3.559 ± 0.078	0.598PheHis: 0.598 ± 0.034	2.679PheIle: 2.679 ± 0.072	1.283PheLys: 1.283 ± 0.053	3.251PheLeu: 3.251 ± 0.071	0.99PheMet: 0.99 ± 0.041	1.42PheAsn: 1.42 ± 0.045	1.701PhePro: 1.701 ± 0.053	0.592PheGln: 0.592 ± 0.026	2.854PheArg: 2.854 ± 0.075	3.666PheSer: 3.666 ± 0.076	2.642PheThr: 2.642 ± 0.062	2.984PheVal: 2.984 ± 0.071	0.367PheTrp: 0.367 ± 0.028	1.598PheTyr: 1.598 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	5.788GlyAla: 5.788 ± 0.095	1.508GlyCys: 1.508 ± 0.054	4.615GlyAsp: 4.615 ± 0.098	5.39GlyGlu: 5.39 ± 0.101	3.657GlyPhe: 3.657 ± 0.07	6.192GlyGly: 6.192 ± 0.132	1.289GlyHis: 1.289 ± 0.044	6.533GlyIle: 6.533 ± 0.106	6.021GlyLys: 6.021 ± 0.086	5.842GlyLeu: 5.842 ± 0.099	2.503GlyMet: 2.503 ± 0.061	3.53GlyAsn: 3.53 ± 0.087	1.038GlyPro: 1.038 ± 0.041	1.702GlyGln: 1.702 ± 0.05	4.068GlyArg: 4.068 ± 0.085	4.825GlySer: 4.825 ± 0.092	5.138GlyThr: 5.138 ± 0.104	5.237GlyVal: 5.237 ± 0.101	0.784GlyTrp: 0.784 ± 0.032	3.717GlyTyr: 3.717 ± 0.075	0.002GlyXaa: 0.002 ± 0.002
His	1.106HisAla: 1.106 ± 0.045	0.376HisCys: 0.376 ± 0.025	0.969HisAsp: 0.969 ± 0.045	0.879HisGlu: 0.879 ± 0.039	0.75HisPhe: 0.75 ± 0.039	1.543HisGly: 1.543 ± 0.054	0.382HisHis: 0.382 ± 0.054	1.317HisIle: 1.317 ± 0.047	0.661HisLys: 0.661 ± 0.034	1.193HisLeu: 1.193 ± 0.044	0.404HisMet: 0.404 ± 0.027	0.598HisAsn: 0.598 ± 0.027	0.931HisPro: 0.931 ± 0.042	0.315HisGln: 0.315 ± 0.02	1.029HisArg: 1.029 ± 0.047	1.001HisSer: 1.001 ± 0.036	0.925HisThr: 0.925 ± 0.039	0.927HisVal: 0.927 ± 0.041	0.156HisTrp: 0.156 ± 0.015	0.663HisTyr: 0.663 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.859IleAla: 5.859 ± 0.099	1.29IleCys: 1.29 ± 0.045	4.131IleAsp: 4.131 ± 0.087	3.715IleGlu: 3.715 ± 0.08	2.617IlePhe: 2.617 ± 0.069	4.849IleGly: 4.849 ± 0.099	0.967IleHis: 0.967 ± 0.037	4.586IleIle: 4.586 ± 0.093	3.747IleLys: 3.747 ± 0.073	5.348IleLeu: 5.348 ± 0.103	1.63IleMet: 1.63 ± 0.054	2.724IleAsn: 2.724 ± 0.07	3.353IlePro: 3.353 ± 0.072	1.104IleGln: 1.104 ± 0.047	3.551IleArg: 3.551 ± 0.077	5.375IleSer: 5.375 ± 0.091	4.489IleThr: 4.489 ± 0.087	4.415IleVal: 4.415 ± 0.088	0.5IleTrp: 0.5 ± 0.029	2.255IleTyr: 2.255 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.683LysAla: 4.683 ± 0.098	0.805LysCys: 0.805 ± 0.037	3.033LysAsp: 3.033 ± 0.085	4.019LysGlu: 4.019 ± 0.091	1.773LysPhe: 1.773 ± 0.053	3.186LysGly: 3.186 ± 0.07	0.985LysHis: 0.985 ± 0.038	3.961LysIle: 3.961 ± 0.087	5.201LysLys: 5.201 ± 0.097	5.183LysLeu: 5.183 ± 0.089	1.803LysMet: 1.803 ± 0.054	3.224LysAsn: 3.224 ± 0.073	2.434LysPro: 2.434 ± 0.062	1.519LysGln: 1.519 ± 0.053	3.173LysArg: 3.173 ± 0.07	3.352LysSer: 3.352 ± 0.07	3.807LysThr: 3.807 ± 0.078	3.161LysVal: 3.161 ± 0.073	0.506LysTrp: 0.506 ± 0.028	2.761LysTyr: 2.761 ± 0.065	0.002LysXaa: 0.002 ± 0.001
Leu	6.918LeuAla: 6.918 ± 0.114	1.879LeuCys: 1.879 ± 0.062	5.102LeuAsp: 5.102 ± 0.086	4.779LeuGlu: 4.779 ± 0.098	3.966LeuPhe: 3.966 ± 0.09	5.492LeuGly: 5.492 ± 0.114	1.441LeuHis: 1.441 ± 0.053	5.549LeuIle: 5.549 ± 0.118	4.83LeuLys: 4.83 ± 0.081	7.191LeuLeu: 7.191 ± 0.129	2.217LeuMet: 2.217 ± 0.062	3.009LeuAsn: 3.009 ± 0.075	4.089LeuPro: 4.089 ± 0.079	1.454LeuGln: 1.454 ± 0.048	4.6LeuArg: 4.6 ± 0.088	7.122LeuSer: 7.122 ± 0.1	5.305LeuThr: 5.305 ± 0.102	5.046LeuVal: 5.046 ± 0.093	0.736LeuTrp: 0.736 ± 0.038	3.285LeuTyr: 3.285 ± 0.067	0.003LeuXaa: 0.003 ± 0.002
Met	1.896MetAla: 1.896 ± 0.052	0.34MetCys: 0.34 ± 0.024	1.181MetAsp: 1.181 ± 0.039	1.271MetGlu: 1.271 ± 0.042	0.984MetPhe: 0.984 ± 0.035	1.543MetGly: 1.543 ± 0.049	0.367MetHis: 0.367 ± 0.025	1.836MetIle: 1.836 ± 0.056	2.614MetLys: 2.614 ± 0.061	2.797MetLeu: 2.797 ± 0.073	0.688MetMet: 0.688 ± 0.033	1.457MetAsn: 1.457 ± 0.05	1.175MetPro: 1.175 ± 0.039	0.604MetGln: 0.604 ± 0.03	1.373MetArg: 1.373 ± 0.047	1.785MetSer: 1.785 ± 0.05	2.073MetThr: 2.073 ± 0.059	1.217MetVal: 1.217 ± 0.043	0.251MetTrp: 0.251 ± 0.019	0.852MetTyr: 0.852 ± 0.035	0.002MetXaa: 0.002 ± 0.002
Asn	3.278AsnAla: 3.278 ± 0.083	0.661AsnCys: 0.661 ± 0.042	2.342AsnAsp: 2.342 ± 0.068	2.294AsnGlu: 2.294 ± 0.059	1.46AsnPhe: 1.46 ± 0.046	4.152AsnGly: 4.152 ± 0.084	0.605AsnHis: 0.605 ± 0.032	2.937AsnIle: 2.937 ± 0.068	1.719AsnLys: 1.719 ± 0.051	2.976AsnLeu: 2.976 ± 0.072	1.095AsnMet: 1.095 ± 0.04	1.622AsnAsn: 1.622 ± 0.056	1.905AsnPro: 1.905 ± 0.054	0.823AsnGln: 0.823 ± 0.041	2.513AsnArg: 2.513 ± 0.067	2.485AsnSer: 2.485 ± 0.068	2.326AsnThr: 2.326 ± 0.064	2.546AsnVal: 2.546 ± 0.061	0.306AsnTrp: 0.306 ± 0.021	1.595AsnTyr: 1.595 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	3.427ProAla: 3.427 ± 0.071	0.59ProCys: 0.59 ± 0.03	3.608ProAsp: 3.608 ± 0.074	4.131ProGlu: 4.131 ± 0.082	1.582ProPhe: 1.582 ± 0.045	2.979ProGly: 2.979 ± 0.075	0.706ProHis: 0.706 ± 0.032	1.795ProIle: 1.795 ± 0.054	1.783ProLys: 1.783 ± 0.057	2.853ProLeu: 2.853 ± 0.078	0.814ProMet: 0.814 ± 0.033	1.031ProAsn: 1.031 ± 0.047	1.272ProPro: 1.272 ± 0.051	0.995ProGln: 0.995 ± 0.038	1.274ProArg: 1.274 ± 0.04	2.289ProSer: 2.289 ± 0.059	1.962ProThr: 1.962 ± 0.054	3.605ProVal: 3.605 ± 0.079	0.317ProTrp: 0.317 ± 0.022	1.544ProTyr: 1.544 ± 0.048	0.005ProXaa: 0.005 ± 0.003
Gln	1.552GlnAla: 1.552 ± 0.045	0.276GlnCys: 0.276 ± 0.02	0.876GlnAsp: 0.876 ± 0.038	1.077GlnGlu: 1.077 ± 0.047	0.852GlnPhe: 0.852 ± 0.036	1.124GlnGly: 1.124 ± 0.042	0.359GlnHis: 0.359 ± 0.022	1.543GlnIle: 1.543 ± 0.05	1.463GlnLys: 1.463 ± 0.058	1.96GlnLeu: 1.96 ± 0.052	0.631GlnMet: 0.631 ± 0.031	1.062GlnAsn: 1.062 ± 0.047	0.831GlnPro: 0.831 ± 0.034	0.535GlnGln: 0.535 ± 0.035	1.104GlnArg: 1.104 ± 0.041	1.308GlnSer: 1.308 ± 0.048	1.233GlnThr: 1.233 ± 0.045	1.196GlnVal: 1.196 ± 0.043	0.209GlnTrp: 0.209 ± 0.02	0.973GlnTyr: 0.973 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	4.001ArgAla: 4.001 ± 0.087	0.873ArgCys: 0.873 ± 0.036	3.174ArgAsp: 3.174 ± 0.072	4.008ArgGlu: 4.008 ± 0.075	2.447ArgPhe: 2.447 ± 0.065	3.398ArgGly: 3.398 ± 0.081	1.064ArgHis: 1.064 ± 0.041	4.032ArgIle: 4.032 ± 0.088	3.367ArgLys: 3.367 ± 0.071	5.359ArgLeu: 5.359 ± 0.109	1.54ArgMet: 1.54 ± 0.049	2.371ArgAsn: 2.371 ± 0.07	1.652ArgPro: 1.652 ± 0.054	1.393ArgGln: 1.393 ± 0.047	3.403ArgArg: 3.403 ± 0.087	2.847ArgSer: 2.847 ± 0.067	2.8ArgThr: 2.8 ± 0.064	3.377ArgVal: 3.377 ± 0.07	0.442ArgTrp: 0.442 ± 0.025	2.339ArgTyr: 2.339 ± 0.057	0.002ArgXaa: 0.002 ± 0.002
Ser	6.286SerAla: 6.286 ± 0.111	1.164SerCys: 1.164 ± 0.043	4.906SerAsp: 4.906 ± 0.098	4.573SerGlu: 4.573 ± 0.09	2.931SerPhe: 2.931 ± 0.074	6.868SerGly: 6.868 ± 0.122	0.988SerHis: 0.988 ± 0.04	3.27SerIle: 3.27 ± 0.075	2.776SerLys: 2.776 ± 0.067	5.637SerLeu: 5.637 ± 0.101	1.498SerMet: 1.498 ± 0.045	1.767SerAsn: 1.767 ± 0.056	2.467SerPro: 2.467 ± 0.059	1.328SerGln: 1.328 ± 0.047	3.284SerArg: 3.284 ± 0.08	3.856SerSer: 3.856 ± 0.101	2.787SerThr: 2.787 ± 0.064	5.755SerVal: 5.755 ± 0.11	0.545SerTrp: 0.545 ± 0.027	2.486SerTyr: 2.486 ± 0.069	0.003SerXaa: 0.003 ± 0.002
Thr	5.902ThrAla: 5.902 ± 0.093	0.69ThrCys: 0.69 ± 0.03	4.33ThrAsp: 4.33 ± 0.09	4.355ThrGlu: 4.355 ± 0.086	2.153ThrPhe: 2.153 ± 0.055	5.644ThrGly: 5.644 ± 0.096	0.933ThrHis: 0.933 ± 0.036	2.509ThrIle: 2.509 ± 0.069	2.588ThrLys: 2.588 ± 0.072	4.807ThrLeu: 4.807 ± 0.092	1.133ThrMet: 1.133 ± 0.043	1.652ThrAsn: 1.652 ± 0.049	2.606ThrPro: 2.606 ± 0.067	1.008ThrGln: 1.008 ± 0.036	2.037ThrArg: 2.037 ± 0.053	3.017ThrSer: 3.017 ± 0.076	2.707ThrThr: 2.707 ± 0.083	7.092ThrVal: 7.092 ± 0.127	0.425ThrTrp: 0.425 ± 0.025	2.115ThrTyr: 2.115 ± 0.065	0.0ThrXaa: 0.0 ± 0.0
Val	4.875ValAla: 4.875 ± 0.1	1.648ValCys: 1.648 ± 0.054	3.566ValAsp: 3.566 ± 0.069	3.278ValGlu: 3.278 ± 0.072	3.508ValPhe: 3.508 ± 0.079	4.903ValGly: 4.903 ± 0.099	1.005ValHis: 1.005 ± 0.043	5.605ValIle: 5.605 ± 0.101	4.193ValLys: 4.193 ± 0.084	6.311ValLeu: 6.311 ± 0.11	2.097ValMet: 2.097 ± 0.061	2.854ValAsn: 2.854 ± 0.061	3.165ValPro: 3.165 ± 0.067	1.172ValGln: 1.172 ± 0.041	4.028ValArg: 4.028 ± 0.079	5.889ValSer: 5.889 ± 0.113	5.214ValThr: 5.214 ± 0.093	4.504ValVal: 4.504 ± 0.114	0.669ValTrp: 0.669 ± 0.031	2.835ValTyr: 2.835 ± 0.074	0.0ValXaa: 0.0 ± 0.0
Trp	0.517TrpAla: 0.517 ± 0.031	0.218TrpCys: 0.218 ± 0.018	0.569TrpAsp: 0.569 ± 0.027	0.544TrpGlu: 0.544 ± 0.03	0.428TrpPhe: 0.428 ± 0.027	0.61TrpGly: 0.61 ± 0.031	0.198TrpHis: 0.198 ± 0.017	0.485TrpIle: 0.485 ± 0.026	0.497TrpLys: 0.497 ± 0.029	0.859TrpLeu: 0.859 ± 0.036	0.222TrpMet: 0.222 ± 0.021	0.464TrpAsn: 0.464 ± 0.033	0.161TrpPro: 0.161 ± 0.017	0.334TrpGln: 0.334 ± 0.023	0.448TrpArg: 0.448 ± 0.026	0.527TrpSer: 0.527 ± 0.027	0.475TrpThr: 0.475 ± 0.03	0.479TrpVal: 0.479 ± 0.028	0.156TrpTrp: 0.156 ± 0.015	0.448TrpTyr: 0.448 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.066TyrAla: 3.066 ± 0.062	0.775TyrCys: 0.775 ± 0.041	2.899TyrAsp: 2.899 ± 0.074	2.437TyrGlu: 2.437 ± 0.064	1.711TyrPhe: 1.711 ± 0.043	3.323TyrGly: 3.323 ± 0.067	0.741TyrHis: 0.741 ± 0.038	2.841TyrIle: 2.841 ± 0.074	1.681TyrLys: 1.681 ± 0.051	3.497TyrLeu: 3.497 ± 0.079	0.897TyrMet: 0.897 ± 0.036	1.646TyrAsn: 1.646 ± 0.052	1.705TyrPro: 1.705 ± 0.05	0.802TyrGln: 0.802 ± 0.036	2.724TyrArg: 2.724 ± 0.057	2.77TyrSer: 2.77 ± 0.063	2.549TyrThr: 2.549 ± 0.086	2.524TyrVal: 2.524 ± 0.067	0.353TyrTrp: 0.353 ± 0.024	1.84TyrTyr: 1.84 ± 0.075	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.002XaaPhe: 0.002 ± 0.001	0.002XaaGly: 0.002 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.005XaaIle: 0.005 ± 0.003	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.002XaaArg: 0.002 ± 0.002	0.002XaaSer: 0.002 ± 0.002	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.026XaaXaa: 0.026 ± 0.009

Statistics based on 2248 proteins (665660 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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