Janibacter indicus
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3139 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3MH18|A0A1L3MH18_9MICO Amino acid/amide ABC transporter substrate-binding protein HAAT family OS=Janibacter indicus OX=857417 GN=ASJ30_08340 PE=4 SV=1
MM1 pKa = 8.07 DD2 pKa = 4.72 LTRR5 pKa = 11.84 PTAPAPYY12 pKa = 10.22 DD13 pKa = 4.02 LLPPTATFEE22 pKa = 4.33 VTSEE26 pKa = 4.18 SFTDD30 pKa = 4.63 GDD32 pKa = 4.86 DD33 pKa = 3.73 LPEE36 pKa = 4.27 AQVFDD41 pKa = 3.75 DD42 pKa = 3.87 WGFSGGNLSPQLSWSGAPEE61 pKa = 4.05 GTKK64 pKa = 10.49 GYY66 pKa = 10.58 AVSCFDD72 pKa = 4.78 PDD74 pKa = 3.49 APTPAGFWHH83 pKa = 5.87 WTVVGLPADD92 pKa = 3.97 TTSLPEE98 pKa = 3.98 GAGVEE103 pKa = 4.65 GGAQLPEE110 pKa = 4.36 GAFMTATDD118 pKa = 4.05 YY119 pKa = 8.59 GTKK122 pKa = 10.38 AFGGAAPPQGDD133 pKa = 3.19 IAHH136 pKa = 6.77 RR137 pKa = 11.84 YY138 pKa = 7.81 VFAVHH143 pKa = 7.0 ALDD146 pKa = 4.37 TDD148 pKa = 3.85 DD149 pKa = 6.35 LGIDD153 pKa = 3.98 DD154 pKa = 5.19 SVSCTVAAFNYY165 pKa = 8.8 LQHH168 pKa = 6.57 TIGRR172 pKa = 11.84 AVITGMYY179 pKa = 9.99 RR180 pKa = 11.84 SS181 pKa = 3.72
Molecular weight: 18.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.872
IPC_protein 3.872
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.253
Thurlkill 3.706
EMBOSS 3.834
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|A0A1L3MKP9|A0A1L3MKP9_9MICO Uncharacterized protein OS=Janibacter indicus OX=857417 GN=ASJ30_16145 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSNRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.45 GRR40 pKa = 11.84 SKK42 pKa = 10.62 ISAA45 pKa = 3.55
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3139
0
3139
1004716
32
1594
320.1
34.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.905 ± 0.065
0.676 ± 0.011
6.605 ± 0.036
6.018 ± 0.046
2.598 ± 0.026
9.331 ± 0.041
2.344 ± 0.024
3.669 ± 0.029
2.033 ± 0.034
10.109 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.913 ± 0.019
1.562 ± 0.023
5.492 ± 0.034
2.842 ± 0.025
7.641 ± 0.049
5.28 ± 0.032
6.319 ± 0.032
9.338 ± 0.043
1.543 ± 0.02
1.783 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here