Gordonia phage BetterKatz
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142KC16|A0A142KC16_9CAUD Head-to-tail connector protein OS=Gordonia phage BetterKatz OX=1821551 GN=14 PE=4 SV=1
MM1 pKa = 7.74 LGRR4 pKa = 11.84 EE5 pKa = 4.37 TVRR8 pKa = 11.84 LRR10 pKa = 11.84 LPAGTTPDD18 pKa = 4.15 GDD20 pKa = 4.26 PLPIAVPVEE29 pKa = 4.15 VEE31 pKa = 4.01 SCLVEE36 pKa = 4.22 VLGGADD42 pKa = 3.64 LVEE45 pKa = 4.74 LGRR48 pKa = 11.84 DD49 pKa = 3.46 GATGGVRR56 pKa = 11.84 VYY58 pKa = 10.31 MPITSGVTAQHH69 pKa = 6.13 DD70 pKa = 4.09 VEE72 pKa = 5.07 VRR74 pKa = 11.84 GRR76 pKa = 11.84 WYY78 pKa = 10.13 EE79 pKa = 3.78 IVGDD83 pKa = 4.31 PEE85 pKa = 4.78 PNLNDD90 pKa = 3.75 DD91 pKa = 4.8 PEE93 pKa = 5.31 LSGYY97 pKa = 10.67 VLTCTRR103 pKa = 11.84 GEE105 pKa = 4.3 GG106 pKa = 3.39
Molecular weight: 11.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.34
IPC2_protein 4.279
IPC_protein 4.177
Toseland 3.999
ProMoST 4.304
Dawson 4.126
Bjellqvist 4.291
Wikipedia 4.024
Rodwell 4.012
Grimsley 3.91
Solomon 4.126
Lehninger 4.075
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.037
Sillero 4.291
Patrickios 3.63
IPC_peptide 4.126
IPC2_peptide 4.279
IPC2.peptide.svr19 4.21
Protein with the highest isoelectric point:
>tr|A0A142KC17|A0A142KC17_9CAUD Head-to-tail connector protein OS=Gordonia phage BetterKatz OX=1821551 GN=15 PE=4 SV=1
MM1 pKa = 6.99 VRR3 pKa = 11.84 VLADD7 pKa = 3.15 PMPRR11 pKa = 11.84 VRR13 pKa = 11.84 AWLTDD18 pKa = 3.4 NLVEE22 pKa = 4.24 AEE24 pKa = 4.26 VRR26 pKa = 11.84 ADD28 pKa = 3.96 VPPNWTLAAATRR40 pKa = 11.84 PLVVVADD47 pKa = 4.35 DD48 pKa = 4.71 GGPVDD53 pKa = 3.35 WPVRR57 pKa = 11.84 SEE59 pKa = 3.64 HH60 pKa = 6.27 TIRR63 pKa = 11.84 LVARR67 pKa = 11.84 AKK69 pKa = 10.62 SRR71 pKa = 11.84 TEE73 pKa = 3.19 ARR75 pKa = 11.84 TTVRR79 pKa = 11.84 LAAGRR84 pKa = 11.84 LHH86 pKa = 5.6 TAKK89 pKa = 9.79 LTGIVVRR96 pKa = 11.84 RR97 pKa = 11.84 AGGSVIEE104 pKa = 4.56 STDD107 pKa = 3.37 KK108 pKa = 11.05 ATGAYY113 pKa = 8.52 LASVLVPIQARR124 pKa = 11.84 TVEE127 pKa = 4.18 LL128 pKa = 3.82
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.443
IPC_protein 10.57
Toseland 10.833
ProMoST 10.847
Dawson 10.877
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 10.833
Grimsley 10.906
Solomon 11.155
Lehninger 11.096
Nozaki 10.804
DTASelect 10.687
Thurlkill 10.818
EMBOSS 11.272
Sillero 10.833
Patrickios 10.628
IPC_peptide 11.155
IPC2_peptide 9.78
IPC2.peptide.svr19 8.967
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
16056
37
1596
214.1
23.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.961 ± 0.445
0.878 ± 0.122
7.15 ± 0.228
5.313 ± 0.27
2.566 ± 0.176
9.274 ± 0.472
2.018 ± 0.192
4.03 ± 0.183
2.803 ± 0.278
8.041 ± 0.252
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.75 ± 0.114
2.74 ± 0.148
5.749 ± 0.328
3.002 ± 0.194
7.598 ± 0.426
5.306 ± 0.309
6.932 ± 0.296
7.947 ± 0.288
2.037 ± 0.1
1.906 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here