Vitreoscilla sp. (strain C1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Vitreoscilla; unclassified Vitreoscilla

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2051 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0PP59|A0A2L0PP59_VITS1 Uncharacterized protein OS=Vitreoscilla sp. (strain C1) OX=96942 GN=ADP71_27020 PE=4 SV=2
MM1 pKa = 6.87VWCLVSGLVLSACADD16 pKa = 3.57SSEE19 pKa = 4.34VTAQAPSHH27 pKa = 6.31SSQVTASASTPLSQVSEE44 pKa = 4.4PNATQVSAEE53 pKa = 4.24ILSSSAVANIQTEE66 pKa = 4.02EE67 pKa = 3.97SRR69 pKa = 11.84NVSVRR74 pKa = 11.84EE75 pKa = 3.76FNPADD80 pKa = 4.44FPDD83 pKa = 3.71ACNDD87 pKa = 3.76YY88 pKa = 10.63IDD90 pKa = 5.69EE91 pKa = 4.16VLQCIKK97 pKa = 11.06VMDD100 pKa = 3.81QAGMEE105 pKa = 4.59GTASMQMQLSEE116 pKa = 4.41MQASWLNTSDD126 pKa = 5.04SNALNQTCKK135 pKa = 10.49LALLDD140 pKa = 4.18VEE142 pKa = 5.66PVLQDD147 pKa = 3.65LGCC150 pKa = 4.44

Molecular weight: 15.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0PNG1|A0A2L0PNG1_VITS1 Cytochrome P450 OS=Vitreoscilla sp. (strain C1) OX=96942 GN=ADP71_22770 PE=3 SV=2
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTKK11 pKa = 10.59RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2051

0

2051

609036

39

2078

296.9

32.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.924 ± 0.057

1.022 ± 0.017

5.275 ± 0.042

5.608 ± 0.056

3.849 ± 0.04

6.925 ± 0.06

2.617 ± 0.03

5.963 ± 0.045

4.785 ± 0.042

10.378 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.028

4.122 ± 0.051

3.966 ± 0.032

5.594 ± 0.064

4.513 ± 0.04

5.692 ± 0.039

5.241 ± 0.042

7.406 ± 0.054

1.398 ± 0.026

2.898 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski