Gemmatimonas sp. TET16

Taxonomy: cellular organisms; Bacteria; FCB group; Gemmatimonadetes; Gemmatimonadetes; Gemmatimonadales; Gemmatimonadaceae; Gemmatimonas; unclassified Gemmatimonas

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M4IR02|A0A6M4IR02_9BACT 50S ribosomal protein L2 OS=Gemmatimonas sp. TET16 OX=2732249 GN=rplB PE=3 SV=1
MM1 pKa = 6.9GTYY4 pKa = 8.89TAVIEE9 pKa = 4.31RR10 pKa = 11.84DD11 pKa = 3.42QSTGLYY17 pKa = 9.09VGYY20 pKa = 10.7VPGWAGAHH28 pKa = 5.3SQGEE32 pKa = 4.42SLDD35 pKa = 3.68EE36 pKa = 3.99LRR38 pKa = 11.84ANLYY42 pKa = 10.64EE43 pKa = 4.23VMLMLLEE50 pKa = 5.08DD51 pKa = 4.56GEE53 pKa = 4.75PALSTEE59 pKa = 4.41FVGTQTLEE67 pKa = 4.06VAA69 pKa = 4.42

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M4IN66|A0A6M4IN66_9BACT Carotenoid biosynthesis protein OS=Gemmatimonas sp. TET16 OX=2732249 GN=HKW67_02455 PE=4 SV=1
MM1 pKa = 6.91AQSRR5 pKa = 11.84FRR7 pKa = 11.84KK8 pKa = 9.86RR9 pKa = 11.84PTRR12 pKa = 11.84TSSVLLVALGAVAGVALGMLVADD35 pKa = 4.3RR36 pKa = 11.84VGGVDD41 pKa = 4.04GLLLPRR47 pKa = 11.84RR48 pKa = 11.84SRR50 pKa = 11.84QPPAARR56 pKa = 11.84ARR58 pKa = 11.84GLARR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84TPHH67 pKa = 6.16RR68 pKa = 11.84LVRR71 pKa = 4.01

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4350

0

4350

1566969

29

2800

360.2

38.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.998 ± 0.052

0.676 ± 0.01

5.593 ± 0.026

4.88 ± 0.035

3.336 ± 0.021

8.409 ± 0.036

1.943 ± 0.021

4.325 ± 0.023

2.456 ± 0.027

9.874 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.013

2.477 ± 0.026

5.298 ± 0.025

3.172 ± 0.021

7.934 ± 0.039

6.058 ± 0.027

6.339 ± 0.032

8.378 ± 0.028

1.426 ± 0.017

2.149 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski