Amino acid dipepetide frequency for Clostridium sp. CAG:590

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.175AlaAla: 6.175 ± 0.121	1.052AlaCys: 1.052 ± 0.04	4.547AlaAsp: 4.547 ± 0.083	4.798AlaGlu: 4.798 ± 0.094	2.938AlaPhe: 2.938 ± 0.068	5.445AlaGly: 5.445 ± 0.103	0.982AlaHis: 0.982 ± 0.041	5.384AlaIle: 5.384 ± 0.105	5.424AlaLys: 5.424 ± 0.092	6.278AlaLeu: 6.278 ± 0.096	2.557AlaMet: 2.557 ± 0.062	2.848AlaAsn: 2.848 ± 0.061	1.795AlaPro: 1.795 ± 0.061	2.328AlaGln: 2.328 ± 0.057	2.699AlaArg: 2.699 ± 0.069	3.708AlaSer: 3.708 ± 0.077	3.893AlaThr: 3.893 ± 0.09	5.647AlaVal: 5.647 ± 0.104	0.515AlaTrp: 0.515 ± 0.024	2.933AlaTyr: 2.933 ± 0.067	0.0AlaXaa: 0.0 ± 0.0
Cys	0.914CysAla: 0.914 ± 0.038	0.237CysCys: 0.237 ± 0.017	0.859CysAsp: 0.859 ± 0.03	0.851CysGlu: 0.851 ± 0.037	0.583CysPhe: 0.583 ± 0.026	1.384CysGly: 1.384 ± 0.042	0.276CysHis: 0.276 ± 0.021	1.12CysIle: 1.12 ± 0.042	0.952CysLys: 0.952 ± 0.035	1.146CysLeu: 1.146 ± 0.043	0.492CysMet: 0.492 ± 0.028	0.672CysAsn: 0.672 ± 0.031	0.544CysPro: 0.544 ± 0.033	0.357CysGln: 0.357 ± 0.018	0.587CysArg: 0.587 ± 0.03	0.843CysSer: 0.843 ± 0.033	0.63CysThr: 0.63 ± 0.03	1.101CysVal: 1.101 ± 0.031	0.087CysTrp: 0.087 ± 0.011	0.693CysTyr: 0.693 ± 0.035	0.001CysXaa: 0.001 ± 0.001
Asp	4.492AspAla: 4.492 ± 0.091	0.794AspCys: 0.794 ± 0.035	3.289AspAsp: 3.289 ± 0.083	4.712AspGlu: 4.712 ± 0.08	2.467AspPhe: 2.467 ± 0.065	4.429AspGly: 4.429 ± 0.076	1.021AspHis: 1.021 ± 0.035	5.033AspIle: 5.033 ± 0.088	4.175AspLys: 4.175 ± 0.087	4.582AspLeu: 4.582 ± 0.09	2.158AspMet: 2.158 ± 0.052	2.695AspAsn: 2.695 ± 0.064	1.614AspPro: 1.614 ± 0.047	1.685AspGln: 1.685 ± 0.051	2.459AspArg: 2.459 ± 0.058	3.261AspSer: 3.261 ± 0.082	3.478AspThr: 3.478 ± 0.07	4.414AspVal: 4.414 ± 0.082	0.542AspTrp: 0.542 ± 0.027	3.133AspTyr: 3.133 ± 0.072	0.001AspXaa: 0.001 ± 0.001
Glu	4.963GluAla: 4.963 ± 0.083	0.861GluCys: 0.861 ± 0.033	4.933GluAsp: 4.933 ± 0.084	7.266GluGlu: 7.266 ± 0.151	2.4GluPhe: 2.4 ± 0.061	4.123GluGly: 4.123 ± 0.079	1.496GluHis: 1.496 ± 0.053	5.434GluIle: 5.434 ± 0.085	6.06GluLys: 6.06 ± 0.097	6.368GluLeu: 6.368 ± 0.106	2.454GluMet: 2.454 ± 0.057	4.031GluAsn: 4.031 ± 0.072	1.845GluPro: 1.845 ± 0.059	3.73GluGln: 3.73 ± 0.08	3.219GluArg: 3.219 ± 0.071	3.369GluSer: 3.369 ± 0.08	3.687GluThr: 3.687 ± 0.071	4.602GluVal: 4.602 ± 0.089	0.632GluTrp: 0.632 ± 0.032	3.324GluTyr: 3.324 ± 0.077	0.003GluXaa: 0.003 ± 0.002
Phe	2.906PheAla: 2.906 ± 0.06	0.681PheCys: 0.681 ± 0.034	2.566PheAsp: 2.566 ± 0.06	2.549PheGlu: 2.549 ± 0.059	1.662PhePhe: 1.662 ± 0.058	2.841PheGly: 2.841 ± 0.062	0.837PheHis: 0.837 ± 0.036	2.621PheIle: 2.621 ± 0.072	1.889PheLys: 1.889 ± 0.058	3.535PheLeu: 3.535 ± 0.084	1.139PheMet: 1.139 ± 0.037	1.442PheAsn: 1.442 ± 0.048	1.22PhePro: 1.22 ± 0.037	1.172PheGln: 1.172 ± 0.036	1.542PheArg: 1.542 ± 0.051	2.556PheSer: 2.556 ± 0.062	2.188PheThr: 2.188 ± 0.056	3.085PheVal: 3.085 ± 0.074	0.353PheTrp: 0.353 ± 0.023	1.669PheTyr: 1.669 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.536GlyAla: 4.536 ± 0.091	1.128GlyCys: 1.128 ± 0.04	3.475GlyAsp: 3.475 ± 0.071	4.316GlyGlu: 4.316 ± 0.087	2.746GlyPhe: 2.746 ± 0.057	4.141GlyGly: 4.141 ± 0.092	1.16GlyHis: 1.16 ± 0.039	6.142GlyIle: 6.142 ± 0.106	5.588GlyLys: 5.588 ± 0.092	5.346GlyLeu: 5.346 ± 0.086	2.476GlyMet: 2.476 ± 0.055	3.228GlyAsn: 3.228 ± 0.074	1.083GlyPro: 1.083 ± 0.042	2.138GlyGln: 2.138 ± 0.057	2.622GlyArg: 2.622 ± 0.068	3.794GlySer: 3.794 ± 0.067	4.274GlyThr: 4.274 ± 0.089	4.971GlyVal: 4.971 ± 0.084	0.59GlyTrp: 0.59 ± 0.029	3.45GlyTyr: 3.45 ± 0.064	0.004GlyXaa: 0.004 ± 0.002
His	1.116HisAla: 1.116 ± 0.037	0.273HisCys: 0.273 ± 0.019	0.911HisAsp: 0.911 ± 0.037	0.947HisGlu: 0.947 ± 0.042	0.931HisPhe: 0.931 ± 0.04	1.212HisGly: 1.212 ± 0.04	0.36HisHis: 0.36 ± 0.03	1.513HisIle: 1.513 ± 0.046	1.048HisLys: 1.048 ± 0.038	1.512HisLeu: 1.512 ± 0.049	0.562HisMet: 0.562 ± 0.029	0.881HisAsn: 0.881 ± 0.032	0.792HisPro: 0.792 ± 0.035	0.521HisGln: 0.521 ± 0.024	0.638HisArg: 0.638 ± 0.029	0.968HisSer: 0.968 ± 0.033	0.96HisThr: 0.96 ± 0.037	1.251HisVal: 1.251 ± 0.045	0.148HisTrp: 0.148 ± 0.014	0.836HisTyr: 0.836 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.265IleAla: 6.265 ± 0.1	1.388IleCys: 1.388 ± 0.04	4.439IleAsp: 4.439 ± 0.082	5.217IleGlu: 5.217 ± 0.094	2.762IlePhe: 2.762 ± 0.066	5.392IleGly: 5.392 ± 0.089	1.454IleHis: 1.454 ± 0.044	5.38IleIle: 5.38 ± 0.113	4.151IleLys: 4.151 ± 0.083	6.997IleLeu: 6.997 ± 0.116	2.156IleMet: 2.156 ± 0.057	2.832IleAsn: 2.832 ± 0.064	3.043IlePro: 3.043 ± 0.06	2.505IleGln: 2.505 ± 0.063	3.655IleArg: 3.655 ± 0.071	4.678IleSer: 4.678 ± 0.086	4.742IleThr: 4.742 ± 0.087	5.655IleVal: 5.655 ± 0.091	0.639IleTrp: 0.639 ± 0.03	3.23IleTyr: 3.23 ± 0.067	0.001IleXaa: 0.001 ± 0.001
Lys	5.138LysAla: 5.138 ± 0.102	0.712LysCys: 0.712 ± 0.032	4.764LysAsp: 4.764 ± 0.095	7.093LysGlu: 7.093 ± 0.114	1.708LysPhe: 1.708 ± 0.049	4.342LysGly: 4.342 ± 0.084	1.048LysHis: 1.048 ± 0.039	4.82LysIle: 4.82 ± 0.077	6.667LysLys: 6.667 ± 0.127	5.182LysLeu: 5.182 ± 0.087	2.416LysMet: 2.416 ± 0.056	3.764LysAsn: 3.764 ± 0.069	1.852LysPro: 1.852 ± 0.061	2.943LysGln: 2.943 ± 0.062	3.048LysArg: 3.048 ± 0.066	3.237LysSer: 3.237 ± 0.076	3.9LysThr: 3.9 ± 0.092	4.945LysVal: 4.945 ± 0.1	0.583LysTrp: 0.583 ± 0.029	2.914LysTyr: 2.914 ± 0.066	0.0LysXaa: 0.0 ± 0.0
Leu	6.165LeuAla: 6.165 ± 0.085	1.422LeuCys: 1.422 ± 0.046	5.121LeuAsp: 5.121 ± 0.093	6.02LeuGlu: 6.02 ± 0.107	3.617LeuPhe: 3.617 ± 0.088	5.332LeuGly: 5.332 ± 0.094	1.628LeuHis: 1.628 ± 0.044	5.883LeuIle: 5.883 ± 0.103	5.683LeuLys: 5.683 ± 0.087	8.011LeuLeu: 8.011 ± 0.128	2.546LeuMet: 2.546 ± 0.066	3.843LeuAsn: 3.843 ± 0.071	3.047LeuPro: 3.047 ± 0.066	3.281LeuGln: 3.281 ± 0.066	3.298LeuArg: 3.298 ± 0.076	5.949LeuSer: 5.949 ± 0.1	4.925LeuThr: 4.925 ± 0.088	5.551LeuVal: 5.551 ± 0.084	0.594LeuTrp: 0.594 ± 0.03	3.475LeuTyr: 3.475 ± 0.074	0.004LeuXaa: 0.004 ± 0.002
Met	2.372MetAla: 2.372 ± 0.061	0.392MetCys: 0.392 ± 0.024	2.167MetAsp: 2.167 ± 0.05	2.576MetGlu: 2.576 ± 0.062	1.06MetPhe: 1.06 ± 0.041	1.994MetGly: 1.994 ± 0.053	0.532MetHis: 0.532 ± 0.031	2.527MetIle: 2.527 ± 0.065	2.654MetLys: 2.654 ± 0.061	2.844MetLeu: 2.844 ± 0.061	0.991MetMet: 0.991 ± 0.044	1.765MetAsn: 1.765 ± 0.051	1.12MetPro: 1.12 ± 0.04	1.196MetGln: 1.196 ± 0.043	1.274MetArg: 1.274 ± 0.042	1.714MetSer: 1.714 ± 0.051	1.761MetThr: 1.761 ± 0.05	2.167MetVal: 2.167 ± 0.069	0.205MetTrp: 0.205 ± 0.017	1.069MetTyr: 1.069 ± 0.036	0.003MetXaa: 0.003 ± 0.002
Asn	3.351AsnAla: 3.351 ± 0.083	0.647AsnCys: 0.647 ± 0.03	2.566AsnAsp: 2.566 ± 0.067	3.069AsnGlu: 3.069 ± 0.067	1.419AsnPhe: 1.419 ± 0.045	3.656AsnGly: 3.656 ± 0.083	0.828AsnHis: 0.828 ± 0.033	3.652AsnIle: 3.652 ± 0.071	3.009AsnLys: 3.009 ± 0.078	3.723AsnLeu: 3.723 ± 0.072	1.524AsnMet: 1.524 ± 0.039	2.169AsnAsn: 2.169 ± 0.069	1.835AsnPro: 1.835 ± 0.051	1.658AsnGln: 1.658 ± 0.048	2.093AsnArg: 2.093 ± 0.055	2.287AsnSer: 2.287 ± 0.058	2.589AsnThr: 2.589 ± 0.064	3.547AsnVal: 3.547 ± 0.083	0.392AsnTrp: 0.392 ± 0.026	2.021AsnTyr: 2.021 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.113ProAla: 2.113 ± 0.061	0.403ProCys: 0.403 ± 0.023	2.293ProAsp: 2.293 ± 0.061	2.933ProGlu: 2.933 ± 0.06	1.359ProPhe: 1.359 ± 0.045	1.96ProGly: 1.96 ± 0.054	0.497ProHis: 0.497 ± 0.027	2.154ProIle: 2.154 ± 0.06	1.788ProLys: 1.788 ± 0.058	2.42ProLeu: 2.42 ± 0.056	0.833ProMet: 0.833 ± 0.037	1.225ProAsn: 1.225 ± 0.041	0.594ProPro: 0.594 ± 0.03	0.956ProGln: 0.956 ± 0.032	0.78ProArg: 0.78 ± 0.033	1.581ProSer: 1.581 ± 0.049	1.504ProThr: 1.504 ± 0.047	2.793ProVal: 2.793 ± 0.067	0.237ProTrp: 0.237 ± 0.019	1.446ProTyr: 1.446 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.558GlnAla: 2.558 ± 0.068	0.414GlnCys: 0.414 ± 0.025	1.828GlnAsp: 1.828 ± 0.048	2.73GlnGlu: 2.73 ± 0.062	1.312GlnPhe: 1.312 ± 0.037	1.926GlnGly: 1.926 ± 0.049	0.56GlnHis: 0.56 ± 0.028	2.955GlnIle: 2.955 ± 0.057	2.717GlnLys: 2.717 ± 0.062	3.191GlnLeu: 3.191 ± 0.077	1.368GlnMet: 1.368 ± 0.047	1.75GlnAsn: 1.75 ± 0.05	0.917GlnPro: 0.917 ± 0.033	1.484GlnGln: 1.484 ± 0.055	1.308GlnArg: 1.308 ± 0.04	1.864GlnSer: 1.864 ± 0.047	1.984GlnThr: 1.984 ± 0.049	2.271GlnVal: 2.271 ± 0.062	0.308GlnTrp: 0.308 ± 0.021	1.811GlnTyr: 1.811 ± 0.054	0.0GlnXaa: 0.0 ± 0.0
Arg	2.275ArgAla: 2.275 ± 0.06	0.515ArgCys: 0.515 ± 0.025	2.078ArgAsp: 2.078 ± 0.048	3.162ArgGlu: 3.162 ± 0.077	1.631ArgPhe: 1.631 ± 0.051	2.042ArgGly: 2.042 ± 0.057	0.691ArgHis: 0.691 ± 0.03	3.535ArgIle: 3.535 ± 0.073	3.462ArgLys: 3.462 ± 0.066	3.54ArgLeu: 3.54 ± 0.065	1.558ArgMet: 1.558 ± 0.048	2.128ArgAsn: 2.128 ± 0.051	1.064ArgPro: 1.064 ± 0.036	1.688ArgGln: 1.688 ± 0.053	1.902ArgArg: 1.902 ± 0.056	2.036ArgSer: 2.036 ± 0.055	2.087ArgThr: 2.087 ± 0.054	2.598ArgVal: 2.598 ± 0.061	0.344ArgTrp: 0.344 ± 0.021	1.918ArgTyr: 1.918 ± 0.058	0.001ArgXaa: 0.001 ± 0.001
Ser	3.875SerAla: 3.875 ± 0.073	0.738SerCys: 0.738 ± 0.04	3.511SerAsp: 3.511 ± 0.078	3.663SerGlu: 3.663 ± 0.086	2.498SerPhe: 2.498 ± 0.057	4.466SerGly: 4.466 ± 0.095	0.931SerHis: 0.931 ± 0.035	4.073SerIle: 4.073 ± 0.079	3.84SerLys: 3.84 ± 0.077	4.793SerLeu: 4.793 ± 0.088	1.814SerMet: 1.814 ± 0.048	2.648SerAsn: 2.648 ± 0.064	1.49SerPro: 1.49 ± 0.045	1.717SerGln: 1.717 ± 0.049	2.13SerArg: 2.13 ± 0.054	3.302SerSer: 3.302 ± 0.081	3.021SerThr: 3.021 ± 0.065	4.552SerVal: 4.552 ± 0.088	0.411SerTrp: 0.411 ± 0.022	2.566SerTyr: 2.566 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	4.057ThrAla: 4.057 ± 0.076	0.667ThrCys: 0.667 ± 0.03	3.506ThrAsp: 3.506 ± 0.081	4.085ThrGlu: 4.085 ± 0.109	2.316ThrPhe: 2.316 ± 0.057	4.451ThrGly: 4.451 ± 0.085	0.884ThrHis: 0.884 ± 0.034	4.738ThrIle: 4.738 ± 0.086	3.728ThrLys: 3.728 ± 0.082	4.828ThrLeu: 4.828 ± 0.071	1.568ThrMet: 1.568 ± 0.041	2.274ThrAsn: 2.274 ± 0.062	2.021ThrPro: 2.021 ± 0.053	1.701ThrGln: 1.701 ± 0.053	1.925ThrArg: 1.925 ± 0.052	3.134ThrSer: 3.134 ± 0.072	3.51ThrThr: 3.51 ± 0.099	4.596ThrVal: 4.596 ± 0.09	0.429ThrTrp: 0.429 ± 0.027	2.519ThrTyr: 2.519 ± 0.086	0.001ThrXaa: 0.001 ± 0.001
Val	5.351ValAla: 5.351 ± 0.102	1.191ValCys: 1.191 ± 0.046	4.285ValAsp: 4.285 ± 0.078	5.067ValGlu: 5.067 ± 0.093	2.845ValPhe: 2.845 ± 0.066	4.461ValGly: 4.461 ± 0.073	1.116ValHis: 1.116 ± 0.035	5.723ValIle: 5.723 ± 0.099	4.977ValLys: 4.977 ± 0.098	6.552ValLeu: 6.552 ± 0.104	2.142ValMet: 2.142 ± 0.048	3.197ValAsn: 3.197 ± 0.065	2.352ValPro: 2.352 ± 0.06	2.087ValGln: 2.087 ± 0.052	2.793ValArg: 2.793 ± 0.067	4.725ValSer: 4.725 ± 0.089	4.905ValThr: 4.905 ± 0.103	5.502ValVal: 5.502 ± 0.107	0.56ValTrp: 0.56 ± 0.031	3.012ValTyr: 3.012 ± 0.061	0.001ValXaa: 0.001 ± 0.001
Trp	0.454TrpAla: 0.454 ± 0.027	0.122TrpCys: 0.122 ± 0.012	0.443TrpAsp: 0.443 ± 0.026	0.499TrpGlu: 0.499 ± 0.025	0.374TrpPhe: 0.374 ± 0.023	0.508TrpGly: 0.508 ± 0.028	0.143TrpHis: 0.143 ± 0.013	0.705TrpIle: 0.705 ± 0.033	0.704TrpLys: 0.704 ± 0.031	0.749TrpLeu: 0.749 ± 0.032	0.319TrpMet: 0.319 ± 0.02	0.496TrpAsn: 0.496 ± 0.028	0.155TrpPro: 0.155 ± 0.015	0.304TrpGln: 0.304 ± 0.021	0.261TrpArg: 0.261 ± 0.019	0.442TrpSer: 0.442 ± 0.026	0.369TrpThr: 0.369 ± 0.026	0.446TrpVal: 0.446 ± 0.022	0.085TrpTrp: 0.085 ± 0.012	0.393TrpTyr: 0.393 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.868TyrAla: 2.868 ± 0.064	0.693TyrCys: 0.693 ± 0.031	2.853TyrAsp: 2.853 ± 0.071	3.246TyrGlu: 3.246 ± 0.066	1.824TyrPhe: 1.824 ± 0.053	3.103TyrGly: 3.103 ± 0.07	0.938TyrHis: 0.938 ± 0.034	3.188TyrIle: 3.188 ± 0.07	2.557TyrLys: 2.557 ± 0.073	3.798TyrLeu: 3.798 ± 0.076	1.306TyrMet: 1.306 ± 0.039	2.147TyrAsn: 2.147 ± 0.055	1.483TyrPro: 1.483 ± 0.052	1.804TyrGln: 1.804 ± 0.054	2.025TyrArg: 2.025 ± 0.053	2.591TyrSer: 2.591 ± 0.07	2.528TyrThr: 2.528 ± 0.068	3.144TyrVal: 3.144 ± 0.064	0.315TyrTrp: 0.315 ± 0.022	2.351TyrTyr: 2.351 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.005XaaVal: 0.005 ± 0.003	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.022XaaXaa: 0.022 ± 0.007

Statistics based on 2354 proteins (755942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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