Corynebacterium ureicelerivorans
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1837 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077HJ26|A0A077HJ26_9CORY HisKA_3 domain-containing protein OS=Corynebacterium ureicelerivorans OX=401472 GN=CUREI_01290 PE=4 SV=1
MM1 pKa = 7.54 NYY3 pKa = 10.6 DD4 pKa = 4.44 DD5 pKa = 5.78 MMPLDD10 pKa = 5.01 PFADD14 pKa = 4.24 DD15 pKa = 4.78 PNDD18 pKa = 3.74 PASFIDD24 pKa = 4.21 DD25 pKa = 3.93 EE26 pKa = 4.5 EE27 pKa = 4.67 HH28 pKa = 7.52 AEE30 pKa = 3.95 PLSPEE35 pKa = 3.66 EE36 pKa = 3.83 RR37 pKa = 11.84 VAVIQDD43 pKa = 3.88 LSHH46 pKa = 7.09 VRR48 pKa = 11.84 DD49 pKa = 3.83 AQRR52 pKa = 11.84 MLKK55 pKa = 9.96 PRR57 pKa = 11.84 GILGVSFLCEE67 pKa = 4.15 DD68 pKa = 4.5 CDD70 pKa = 3.92 QVHH73 pKa = 6.07 YY74 pKa = 10.52 YY75 pKa = 10.3 DD76 pKa = 4.05 WEE78 pKa = 3.95 IMEE81 pKa = 4.48 QNMIASLNNQLPPVHH96 pKa = 6.58 EE97 pKa = 4.56 PSANPNVEE105 pKa = 5.44 AYY107 pKa = 10.61 VPWDD111 pKa = 3.39 YY112 pKa = 12.19 AMGYY116 pKa = 10.59 LDD118 pKa = 4.04 GLEE121 pKa = 4.1 GRR123 pKa = 4.8
Molecular weight: 14.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A077HG51|A0A077HG51_9CORY DNA replication and repair protein RecF OS=Corynebacterium ureicelerivorans OX=401472 GN=recF PE=3 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.93 RR4 pKa = 11.84 TYY6 pKa = 10.0 QPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.24 HH17 pKa = 4.6 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MQTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVSARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.67 KK38 pKa = 10.5 GRR40 pKa = 11.84 ANLTAA45 pKa = 4.8
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1837
0
1837
572859
40
2375
311.8
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.436 ± 0.076
0.658 ± 0.015
6.42 ± 0.05
6.579 ± 0.048
3.175 ± 0.039
8.586 ± 0.048
2.069 ± 0.028
4.531 ± 0.045
3.053 ± 0.052
9.302 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.026
2.704 ± 0.034
5.003 ± 0.036
3.284 ± 0.031
6.508 ± 0.062
5.17 ± 0.035
5.983 ± 0.041
8.646 ± 0.053
1.352 ± 0.027
2.258 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here