Bitter gourd leaf curl betasatellite
Average proteome isoelectric point is 5.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2VU50|Q2VU50_9VIRU Truncated beta C1 OS=Bitter gourd leaf curl betasatellite OX=316106 GN=beta C1 PE=4 SV=1
MM1 pKa = 7.09 TRR3 pKa = 11.84 SGTNKK8 pKa = 9.62 QGVKK12 pKa = 8.4 FTVDD16 pKa = 3.1 VRR18 pKa = 11.84 IMEE21 pKa = 3.99 NMKK24 pKa = 10.08 IFIHH28 pKa = 6.0 IRR30 pKa = 11.84 IVSTMSPALIRR41 pKa = 11.84 YY42 pKa = 7.63 EE43 pKa = 4.69 GIVKK47 pKa = 8.09 YY48 pKa = 9.04 TYY50 pKa = 11.5 GDD52 pKa = 3.18 MHH54 pKa = 8.1 VPFDD58 pKa = 4.19 FNGFEE63 pKa = 4.42 GNIIANFLFANNGAKK78 pKa = 9.48 IEE80 pKa = 4.34 EE81 pKa = 4.35 IEE83 pKa = 5.08 IEE85 pKa = 5.24 DD86 pKa = 3.55 IVQQ89 pKa = 3.14
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.471
IPC2_protein 5.537
IPC_protein 5.41
Toseland 5.639
ProMoST 5.614
Dawson 5.512
Bjellqvist 5.575
Wikipedia 5.448
Rodwell 5.474
Grimsley 5.715
Solomon 5.512
Lehninger 5.486
Nozaki 5.703
DTASelect 5.855
Thurlkill 5.728
EMBOSS 5.664
Sillero 5.792
Patrickios 4.342
IPC_peptide 5.537
IPC2_peptide 5.792
IPC2.peptide.svr19 5.936
Protein with the highest isoelectric point:
>tr|Q2VU50|Q2VU50_9VIRU Truncated beta C1 OS=Bitter gourd leaf curl betasatellite OX=316106 GN=beta C1 PE=4 SV=1
MM1 pKa = 7.09 TRR3 pKa = 11.84 SGTNKK8 pKa = 9.62 QGVKK12 pKa = 8.4 FTVDD16 pKa = 3.1 VRR18 pKa = 11.84 IMEE21 pKa = 3.99 NMKK24 pKa = 10.08 IFIHH28 pKa = 6.0 IRR30 pKa = 11.84 IVSTMSPALIRR41 pKa = 11.84 YY42 pKa = 7.63 EE43 pKa = 4.69 GIVKK47 pKa = 8.09 YY48 pKa = 9.04 TYY50 pKa = 11.5 GDD52 pKa = 3.18 MHH54 pKa = 8.1 VPFDD58 pKa = 4.19 FNGFEE63 pKa = 4.42 GNIIANFLFANNGAKK78 pKa = 9.48 IEE80 pKa = 4.34 EE81 pKa = 4.35 IEE83 pKa = 5.08 IEE85 pKa = 5.24 DD86 pKa = 3.55 IVQQ89 pKa = 3.14
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.471
IPC2_protein 5.537
IPC_protein 5.41
Toseland 5.639
ProMoST 5.614
Dawson 5.512
Bjellqvist 5.575
Wikipedia 5.448
Rodwell 5.474
Grimsley 5.715
Solomon 5.512
Lehninger 5.486
Nozaki 5.703
DTASelect 5.855
Thurlkill 5.728
EMBOSS 5.664
Sillero 5.792
Patrickios 4.342
IPC_peptide 5.537
IPC2_peptide 5.792
IPC2.peptide.svr19 5.936
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
89
89
89
89.0
10.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.494 ± 0.0
0.0 ± 0.0
4.494 ± 0.0
7.865 ± 0.0
7.865 ± 0.0
7.865 ± 0.0
2.247 ± 0.0
14.607 ± 0.0
5.618 ± 0.0
2.247 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.618 ± 0.0
7.865 ± 0.0
2.247 ± 0.0
2.247 ± 0.0
4.494 ± 0.0
3.371 ± 0.0
5.618 ± 0.0
7.865 ± 0.0
0.0 ± 0.0
3.371 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here