High Plains wheat mosaic emaravirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076VAA0|A0A076VAA0_9VIRU Uncharacterized protein OS=High Plains wheat mosaic emaravirus OX=1980428 PE=4 SV=1
MM1 pKa = 6.95NTIKK5 pKa = 9.95TVIISEE11 pKa = 4.26LEE13 pKa = 4.05KK14 pKa = 11.28NVDD17 pKa = 3.44EE18 pKa = 4.81FLNSYY23 pKa = 10.75LEE25 pKa = 3.89YY26 pKa = 10.78LKK28 pKa = 11.01YY29 pKa = 10.64DD30 pKa = 5.11DD31 pKa = 4.41YY32 pKa = 11.98DD33 pKa = 3.6QYY35 pKa = 11.53CTMIGLYY42 pKa = 10.45DD43 pKa = 3.99EE44 pKa = 5.57LTDD47 pKa = 4.29QEE49 pKa = 5.2SISQIPTKK57 pKa = 10.91YY58 pKa = 10.7SIDD61 pKa = 4.19PINFQKK67 pKa = 9.6FTRR70 pKa = 11.84VLTVAIYY77 pKa = 10.3NYY79 pKa = 10.2DD80 pKa = 3.18VNYY83 pKa = 10.37ILAEE87 pKa = 4.05KK88 pKa = 10.29YY89 pKa = 9.96KK90 pKa = 10.77EE91 pKa = 3.98LFEE94 pKa = 4.22FTNMDD99 pKa = 3.76PDD101 pKa = 4.35FSPKK105 pKa = 9.74YY106 pKa = 10.07RR107 pKa = 11.84FYY109 pKa = 11.59SPIATCSYY117 pKa = 10.28LSQYY121 pKa = 11.46DD122 pKa = 4.44LISEE126 pKa = 4.56SFQQDD131 pKa = 3.18VTKK134 pKa = 10.95LFDD137 pKa = 4.21RR138 pKa = 11.84MHH140 pKa = 6.21KK141 pKa = 9.04QQPGCMLMNQIMVSNLIKK159 pKa = 10.84NLLKK163 pKa = 10.55NVQTIGQDD171 pKa = 3.5DD172 pKa = 4.0SSHH175 pKa = 5.3NN176 pKa = 3.64

Molecular weight: 20.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076V8I4|A0A076V8I4_9VIRU P8 RNA silencing suppressor OS=High Plains wheat mosaic emaravirus OX=1980428 PE=4 SV=1
MM1 pKa = 7.55ALSFKK6 pKa = 10.64NSSGVIKK13 pKa = 10.73AKK15 pKa = 9.53TIKK18 pKa = 10.66DD19 pKa = 3.67GFVTSSDD26 pKa = 3.19IEE28 pKa = 4.55TTVHH32 pKa = 6.36DD33 pKa = 4.54FSYY36 pKa = 10.61EE37 pKa = 4.03KK38 pKa = 10.22PDD40 pKa = 3.64LSSVDD45 pKa = 3.59GFSLKK50 pKa = 10.72SLLSSDD56 pKa = 2.8GWHH59 pKa = 6.18IVVAYY64 pKa = 9.71QSVTNSEE71 pKa = 3.96RR72 pKa = 11.84LNNNKK77 pKa = 9.56KK78 pKa = 10.03NNKK81 pKa = 4.97TQRR84 pKa = 11.84FKK86 pKa = 11.55LFTFDD91 pKa = 3.99IIVIPGLKK99 pKa = 9.55PNKK102 pKa = 9.3SKK104 pKa = 11.29NVVSYY109 pKa = 10.84NRR111 pKa = 11.84FMALCIGMICYY122 pKa = 9.17HH123 pKa = 6.42KK124 pKa = 10.13KK125 pKa = 9.06WKK127 pKa = 9.24VFNWSNKK134 pKa = 9.0RR135 pKa = 11.84YY136 pKa = 9.46EE137 pKa = 4.32DD138 pKa = 3.44NKK140 pKa = 9.82NTINFNEE147 pKa = 5.23DD148 pKa = 3.16DD149 pKa = 4.3DD150 pKa = 4.96FMNKK154 pKa = 9.44LAMSAGFSKK163 pKa = 9.4EE164 pKa = 4.14HH165 pKa = 6.67KK166 pKa = 9.25YY167 pKa = 10.28HH168 pKa = 6.59WFYY171 pKa = 11.25STGFEE176 pKa = 4.17YY177 pKa = 10.72TFDD180 pKa = 3.43IFPAEE185 pKa = 4.39VIAMSLFRR193 pKa = 11.84WSHH196 pKa = 4.55RR197 pKa = 11.84VEE199 pKa = 4.7LKK201 pKa = 10.52VKK203 pKa = 10.36YY204 pKa = 8.33EE205 pKa = 4.08HH206 pKa = 7.03EE207 pKa = 4.63SDD209 pKa = 4.05LVAPMVRR216 pKa = 11.84QVTKK220 pKa = 10.22RR221 pKa = 11.84GNISDD226 pKa = 3.62VMDD229 pKa = 3.61IVGKK233 pKa = 10.24DD234 pKa = 3.43IIAKK238 pKa = 9.76KK239 pKa = 10.32YY240 pKa = 9.96EE241 pKa = 4.43EE242 pKa = 4.13IVKK245 pKa = 10.14DD246 pKa = 3.85RR247 pKa = 11.84SSIGIGTKK255 pKa = 10.68YY256 pKa = 10.99NDD258 pKa = 3.58ILDD261 pKa = 3.82EE262 pKa = 4.8FKK264 pKa = 11.11DD265 pKa = 3.48IFNKK269 pKa = 9.76IDD271 pKa = 3.57SSSLDD276 pKa = 3.53STIKK280 pKa = 10.79NCFNKK285 pKa = 9.66IDD287 pKa = 3.92GEE289 pKa = 4.34

Molecular weight: 33.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

5043

176

2272

630.4

73.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.637 ± 0.242

1.765 ± 0.325

7.079 ± 0.349

6.246 ± 0.451

5.354 ± 0.35

3.193 ± 0.411

2.181 ± 0.193

8.467 ± 0.282

9.478 ± 0.375

8.745 ± 0.77

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.192

7.476 ± 0.26

2.677 ± 0.246

2.796 ± 0.416

3.411 ± 0.333

8.031 ± 0.434

5.513 ± 0.28

5.731 ± 0.531

0.555 ± 0.198

5.79 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski