Amino acid dipepetide frequency for Microbacterium sp. ZXX196

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.67AlaAla: 21.67 ± 0.292
0.757AlaCys: 0.757 ± 0.035
9.402AlaAsp: 9.402 ± 0.146
9.147AlaGlu: 9.147 ± 0.133
4.284AlaPhe: 4.284 ± 0.076
13.397AlaGly: 13.397 ± 0.178
2.757AlaHis: 2.757 ± 0.07
6.255AlaIle: 6.255 ± 0.099
2.466AlaLys: 2.466 ± 0.069
15.066AlaLeu: 15.066 ± 0.185
2.856AlaMet: 2.856 ± 0.057
2.161AlaAsn: 2.161 ± 0.061
7.131AlaPro: 7.131 ± 0.132
3.787AlaGln: 3.787 ± 0.078
11.162AlaArg: 11.162 ± 0.167
7.038AlaSer: 7.038 ± 0.106
7.426AlaThr: 7.426 ± 0.117
11.425AlaVal: 11.425 ± 0.143
2.282AlaTrp: 2.282 ± 0.064
2.69AlaTyr: 2.69 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.719CysAla: 0.719 ± 0.033
0.063CysCys: 0.063 ± 0.009
0.334CysAsp: 0.334 ± 0.022
0.261CysGlu: 0.261 ± 0.018
0.156CysPhe: 0.156 ± 0.015
0.594CysGly: 0.594 ± 0.029
0.122CysHis: 0.122 ± 0.012
0.192CysIle: 0.192 ± 0.016
0.068CysLys: 0.068 ± 0.01
0.4CysLeu: 0.4 ± 0.021
0.067CysMet: 0.067 ± 0.01
0.092CysAsn: 0.092 ± 0.011
0.301CysPro: 0.301 ± 0.021
0.106CysGln: 0.106 ± 0.012
0.334CysArg: 0.334 ± 0.021
0.289CysSer: 0.289 ± 0.019
0.303CysThr: 0.303 ± 0.02
0.463CysVal: 0.463 ± 0.023
0.064CysTrp: 0.064 ± 0.01
0.106CysTyr: 0.106 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
9.737AspAla: 9.737 ± 0.148
0.231AspCys: 0.231 ± 0.016
4.21AspAsp: 4.21 ± 0.086
4.72AspGlu: 4.72 ± 0.086
1.705AspPhe: 1.705 ± 0.045
6.236AspGly: 6.236 ± 0.102
1.132AspHis: 1.132 ± 0.044
2.494AspIle: 2.494 ± 0.065
1.082AspLys: 1.082 ± 0.045
6.167AspLeu: 6.167 ± 0.096
1.013AspMet: 1.013 ± 0.039
0.985AspAsn: 0.985 ± 0.041
4.628AspPro: 4.628 ± 0.086
1.629AspGln: 1.629 ± 0.048
4.432AspArg: 4.432 ± 0.076
2.125AspSer: 2.125 ± 0.057
2.976AspThr: 2.976 ± 0.062
5.7AspVal: 5.7 ± 0.102
0.812AspTrp: 0.812 ± 0.033
1.251AspTyr: 1.251 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
7.986GluAla: 7.986 ± 0.107
0.243GluCys: 0.243 ± 0.02
3.335GluAsp: 3.335 ± 0.073
3.831GluGlu: 3.831 ± 0.09
1.794GluPhe: 1.794 ± 0.046
4.565GluGly: 4.565 ± 0.074
1.403GluHis: 1.403 ± 0.045
3.208GluIle: 3.208 ± 0.077
1.604GluLys: 1.604 ± 0.05
6.289GluLeu: 6.289 ± 0.102
1.113GluMet: 1.113 ± 0.038
1.32GluAsn: 1.32 ± 0.045
2.91GluPro: 2.91 ± 0.07
2.055GluGln: 2.055 ± 0.062
5.944GluArg: 5.944 ± 0.105
2.634GluSer: 2.634 ± 0.065
3.321GluThr: 3.321 ± 0.071
5.02GluVal: 5.02 ± 0.094
0.905GluTrp: 0.905 ± 0.036
1.145GluTyr: 1.145 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
4.535PheAla: 4.535 ± 0.088
0.19PheCys: 0.19 ± 0.016
2.28PheAsp: 2.28 ± 0.054
1.731PheGlu: 1.731 ± 0.053
1.134PhePhe: 1.134 ± 0.044
3.342PheGly: 3.342 ± 0.075
0.579PheHis: 0.579 ± 0.029
1.069PheIle: 1.069 ± 0.036
0.416PheLys: 0.416 ± 0.023
2.924PheLeu: 2.924 ± 0.066
0.47PheMet: 0.47 ± 0.023
0.637PheAsn: 0.637 ± 0.029
1.41PhePro: 1.41 ± 0.044
0.86PheGln: 0.86 ± 0.036
2.016PheArg: 2.016 ± 0.059
1.813PheSer: 1.813 ± 0.048
1.953PheThr: 1.953 ± 0.06
2.881PheVal: 2.881 ± 0.068
0.46PheTrp: 0.46 ± 0.03
0.659PheTyr: 0.659 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
12.07GlyAla: 12.07 ± 0.185
0.561GlyCys: 0.561 ± 0.03
5.43GlyAsp: 5.43 ± 0.091
5.682GlyGlu: 5.682 ± 0.095
3.193GlyPhe: 3.193 ± 0.07
7.819GlyGly: 7.819 ± 0.129
1.781GlyHis: 1.781 ± 0.05
4.562GlyIle: 4.562 ± 0.088
2.006GlyLys: 2.006 ± 0.056
8.566GlyLeu: 8.566 ± 0.115
1.951GlyMet: 1.951 ± 0.053
1.559GlyAsn: 1.559 ± 0.05
3.7GlyPro: 3.7 ± 0.07
2.468GlyGln: 2.468 ± 0.059
6.846GlyArg: 6.846 ± 0.119
5.741GlySer: 5.741 ± 0.105
5.168GlyThr: 5.168 ± 0.082
8.146GlyVal: 8.146 ± 0.113
1.644GlyTrp: 1.644 ± 0.043
2.144GlyTyr: 2.144 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.593HisAla: 2.593 ± 0.064
0.093HisCys: 0.093 ± 0.012
1.285HisAsp: 1.285 ± 0.04
1.139HisGlu: 1.139 ± 0.034
0.575HisPhe: 0.575 ± 0.024
1.878HisGly: 1.878 ± 0.049
0.543HisHis: 0.543 ± 0.033
0.736HisIle: 0.736 ± 0.032
0.295HisLys: 0.295 ± 0.021
1.963HisLeu: 1.963 ± 0.051
0.304HisMet: 0.304 ± 0.018
0.348HisAsn: 0.348 ± 0.02
1.382HisPro: 1.382 ± 0.036
0.482HisGln: 0.482 ± 0.025
1.49HisArg: 1.49 ± 0.047
0.818HisSer: 0.818 ± 0.03
0.989HisThr: 0.989 ± 0.04
1.695HisVal: 1.695 ± 0.048
0.245HisTrp: 0.245 ± 0.018
0.422HisTyr: 0.422 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
7.27IleAla: 7.27 ± 0.103
0.217IleCys: 0.217 ± 0.017
3.387IleAsp: 3.387 ± 0.068
2.851IleGlu: 2.851 ± 0.073
1.144IlePhe: 1.144 ± 0.044
4.309IleGly: 4.309 ± 0.092
0.711IleHis: 0.711 ± 0.029
1.622IleIle: 1.622 ± 0.051
0.786IleLys: 0.786 ± 0.038
3.739IleLeu: 3.739 ± 0.084
0.671IleMet: 0.671 ± 0.033
0.878IleAsn: 0.878 ± 0.035
2.387IlePro: 2.387 ± 0.062
1.045IleGln: 1.045 ± 0.038
2.961IleArg: 2.961 ± 0.065
2.163IleSer: 2.163 ± 0.058
2.402IleThr: 2.402 ± 0.071
4.585IleVal: 4.585 ± 0.088
0.465IleTrp: 0.465 ± 0.025
0.757IleTyr: 0.757 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
2.281LysAla: 2.281 ± 0.065
0.078LysCys: 0.078 ± 0.011
1.088LysAsp: 1.088 ± 0.041
1.015LysGlu: 1.015 ± 0.048
0.512LysPhe: 0.512 ± 0.028
1.525LysGly: 1.525 ± 0.063
0.398LysHis: 0.398 ± 0.023
0.944LysIle: 0.944 ± 0.038
0.953LysLys: 0.953 ± 0.042
1.762LysLeu: 1.762 ± 0.054
0.412LysMet: 0.412 ± 0.023
0.584LysAsn: 0.584 ± 0.029
1.037LysPro: 1.037 ± 0.036
0.686LysGln: 0.686 ± 0.032
1.525LysArg: 1.525 ± 0.046
1.071LysSer: 1.071 ± 0.041
1.289LysThr: 1.289 ± 0.044
1.702LysVal: 1.702 ± 0.051
0.217LysTrp: 0.217 ± 0.016
0.417LysTyr: 0.417 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
15.914LeuAla: 15.914 ± 0.194
0.475LeuCys: 0.475 ± 0.022
6.363LeuAsp: 6.363 ± 0.104
5.479LeuGlu: 5.479 ± 0.098
2.928LeuPhe: 2.928 ± 0.075
9.039LeuGly: 9.039 ± 0.127
1.606LeuHis: 1.606 ± 0.046
3.898LeuIle: 3.898 ± 0.089
1.666LeuLys: 1.666 ± 0.053
9.007LeuLeu: 9.007 ± 0.147
1.551LeuMet: 1.551 ± 0.058
1.681LeuAsn: 1.681 ± 0.05
5.188LeuPro: 5.188 ± 0.094
2.281LeuGln: 2.281 ± 0.051
6.811LeuArg: 6.811 ± 0.109
5.141LeuSer: 5.141 ± 0.087
5.734LeuThr: 5.734 ± 0.095
9.533LeuVal: 9.533 ± 0.144
1.229LeuTrp: 1.229 ± 0.045
1.555LeuTyr: 1.555 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.199MetAla: 2.199 ± 0.057
0.11MetCys: 0.11 ± 0.011
0.799MetAsp: 0.799 ± 0.035
0.667MetGlu: 0.667 ± 0.03
0.589MetPhe: 0.589 ± 0.031
1.479MetGly: 1.479 ± 0.047
0.327MetHis: 0.327 ± 0.021
0.884MetIle: 0.884 ± 0.034
0.48MetLys: 0.48 ± 0.025
1.791MetLeu: 1.791 ± 0.051
0.361MetMet: 0.361 ± 0.024
0.472MetAsn: 0.472 ± 0.025
1.057MetPro: 1.057 ± 0.037
0.514MetGln: 0.514 ± 0.027
1.527MetArg: 1.527 ± 0.045
1.521MetSer: 1.521 ± 0.04
1.762MetThr: 1.762 ± 0.051
1.245MetVal: 1.245 ± 0.043
0.228MetTrp: 0.228 ± 0.017
0.311MetTyr: 0.311 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.314AsnAla: 2.314 ± 0.062
0.105AsnCys: 0.105 ± 0.009
1.153AsnAsp: 1.153 ± 0.046
1.001AsnGlu: 1.001 ± 0.041
0.569AsnPhe: 0.569 ± 0.029
1.758AsnGly: 1.758 ± 0.054
0.33AsnHis: 0.33 ± 0.021
0.802AsnIle: 0.802 ± 0.036
0.436AsnLys: 0.436 ± 0.027
1.752AsnLeu: 1.752 ± 0.048
0.362AsnMet: 0.362 ± 0.026
0.465AsnAsn: 0.465 ± 0.028
1.482AsnPro: 1.482 ± 0.046
0.541AsnGln: 0.541 ± 0.028
1.204AsnArg: 1.204 ± 0.043
0.845AsnSer: 0.845 ± 0.036
1.005AsnThr: 1.005 ± 0.035
1.637AsnVal: 1.637 ± 0.049
0.264AsnTrp: 0.264 ± 0.017
0.445AsnTyr: 0.445 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.586ProAla: 7.586 ± 0.124
0.208ProCys: 0.208 ± 0.019
3.76ProAsp: 3.76 ± 0.074
4.113ProGlu: 4.113 ± 0.073
1.677ProPhe: 1.677 ± 0.045
5.086ProGly: 5.086 ± 0.086
1.134ProHis: 1.134 ± 0.046
1.946ProIle: 1.946 ± 0.048
0.914ProLys: 0.914 ± 0.034
4.787ProLeu: 4.787 ± 0.088
0.863ProMet: 0.863 ± 0.034
0.952ProAsn: 0.952 ± 0.042
2.095ProPro: 2.095 ± 0.061
1.435ProGln: 1.435 ± 0.044
3.936ProArg: 3.936 ± 0.084
2.775ProSer: 2.775 ± 0.067
3.093ProThr: 3.093 ± 0.059
4.565ProVal: 4.565 ± 0.069
0.865ProTrp: 0.865 ± 0.035
1.034ProTyr: 1.034 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
3.811GlnAla: 3.811 ± 0.076
0.112GlnCys: 0.112 ± 0.013
1.282GlnAsp: 1.282 ± 0.039
1.265GlnGlu: 1.265 ± 0.045
0.837GlnPhe: 0.837 ± 0.031
2.047GlnGly: 2.047 ± 0.058
0.562GlnHis: 0.562 ± 0.028
1.393GlnIle: 1.393 ± 0.044
0.748GlnLys: 0.748 ± 0.036
2.865GlnLeu: 2.865 ± 0.07
0.549GlnMet: 0.549 ± 0.027
0.685GlnAsn: 0.685 ± 0.038
1.3GlnPro: 1.3 ± 0.039
1.058GlnGln: 1.058 ± 0.042
2.246GlnArg: 2.246 ± 0.062
1.277GlnSer: 1.277 ± 0.042
1.47GlnThr: 1.47 ± 0.043
2.35GlnVal: 2.35 ± 0.058
0.387GlnTrp: 0.387 ± 0.025
0.526GlnTyr: 0.526 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
11.04ArgAla: 11.04 ± 0.151
0.3ArgCys: 0.3 ± 0.022
4.637ArgAsp: 4.637 ± 0.081
4.985ArgGlu: 4.985 ± 0.089
2.454ArgPhe: 2.454 ± 0.052
6.313ArgGly: 6.313 ± 0.098
1.571ArgHis: 1.571 ± 0.043
3.881ArgIle: 3.881 ± 0.071
1.332ArgLys: 1.332 ± 0.047
7.185ArgLeu: 7.185 ± 0.115
1.628ArgMet: 1.628 ± 0.036
1.182ArgAsn: 1.182 ± 0.044
3.809ArgPro: 3.809 ± 0.081
2.139ArgGln: 2.139 ± 0.054
7.14ArgArg: 7.14 ± 0.132
3.604ArgSer: 3.604 ± 0.066
4.101ArgThr: 4.101 ± 0.073
6.727ArgVal: 6.727 ± 0.105
1.064ArgTrp: 1.064 ± 0.037
1.527ArgTyr: 1.527 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.964SerAla: 6.964 ± 0.097
0.257SerCys: 0.257 ± 0.016
3.226SerAsp: 3.226 ± 0.066
2.902SerGlu: 2.902 ± 0.061
1.811SerPhe: 1.811 ± 0.044
5.599SerGly: 5.599 ± 0.095
0.979SerHis: 0.979 ± 0.032
2.276SerIle: 2.276 ± 0.057
0.97SerLys: 0.97 ± 0.041
4.835SerLeu: 4.835 ± 0.076
1.053SerMet: 1.053 ± 0.04
1.001SerAsn: 1.001 ± 0.035
2.878SerPro: 2.878 ± 0.057
1.272SerGln: 1.272 ± 0.044
3.603SerArg: 3.603 ± 0.068
3.001SerSer: 3.001 ± 0.077
3.034SerThr: 3.034 ± 0.066
4.314SerVal: 4.314 ± 0.085
0.845SerTrp: 0.845 ± 0.033
1.111SerTyr: 1.111 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
7.292ThrAla: 7.292 ± 0.099
0.281ThrCys: 0.281 ± 0.022
3.449ThrAsp: 3.449 ± 0.077
2.909ThrGlu: 2.909 ± 0.064
1.833ThrPhe: 1.833 ± 0.054
5.625ThrGly: 5.625 ± 0.111
1.199ThrHis: 1.199 ± 0.044
2.816ThrIle: 2.816 ± 0.073
1.11ThrLys: 1.11 ± 0.043
5.587ThrLeu: 5.587 ± 0.094
1.019ThrMet: 1.019 ± 0.04
1.084ThrAsn: 1.084 ± 0.042
3.804ThrPro: 3.804 ± 0.07
1.364ThrGln: 1.364 ± 0.042
3.912ThrArg: 3.912 ± 0.084
3.178ThrSer: 3.178 ± 0.063
3.474ThrThr: 3.474 ± 0.074
5.072ThrVal: 5.072 ± 0.096
0.865ThrTrp: 0.865 ± 0.039
1.242ThrTyr: 1.242 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
12.846ValAla: 12.846 ± 0.148
0.528ValCys: 0.528 ± 0.029
5.648ValAsp: 5.648 ± 0.099
5.039ValGlu: 5.039 ± 0.099
2.894ValPhe: 2.894 ± 0.07
7.392ValGly: 7.392 ± 0.109
1.561ValHis: 1.561 ± 0.048
3.962ValIle: 3.962 ± 0.07
1.528ValLys: 1.528 ± 0.05
8.768ValLeu: 8.768 ± 0.123
1.498ValMet: 1.498 ± 0.047
1.569ValAsn: 1.569 ± 0.051
4.645ValPro: 4.645 ± 0.081
1.978ValGln: 1.978 ± 0.056
6.524ValArg: 6.524 ± 0.102
4.98ValSer: 4.98 ± 0.082
5.671ValThr: 5.671 ± 0.096
9.118ValVal: 9.118 ± 0.14
1.279ValTrp: 1.279 ± 0.046
1.541ValTyr: 1.541 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
1.745TrpAla: 1.745 ± 0.055
0.106TrpCys: 0.106 ± 0.01
0.789TrpAsp: 0.789 ± 0.034
0.7TrpGlu: 0.7 ± 0.03
0.562TrpPhe: 0.562 ± 0.028
1.144TrpGly: 1.144 ± 0.045
0.308TrpHis: 0.308 ± 0.021
0.76TrpIle: 0.76 ± 0.035
0.288TrpLys: 0.288 ± 0.02
1.605TrpLeu: 1.605 ± 0.057
0.284TrpMet: 0.284 ± 0.02
0.353TrpAsn: 0.353 ± 0.025
0.697TrpPro: 0.697 ± 0.029
0.52TrpGln: 0.52 ± 0.025
1.369TrpArg: 1.369 ± 0.045
0.869TrpSer: 0.869 ± 0.036
0.85TrpThr: 0.85 ± 0.033
1.166TrpVal: 1.166 ± 0.042
0.343TrpTrp: 0.343 ± 0.022
0.264TrpTyr: 0.264 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.586TyrAla: 2.586 ± 0.058
0.117TyrCys: 0.117 ± 0.012
1.407TyrAsp: 1.407 ± 0.042
1.192TyrGlu: 1.192 ± 0.041
0.71TyrPhe: 0.71 ± 0.032
1.893TyrGly: 1.893 ± 0.05
0.271TyrHis: 0.271 ± 0.018
0.647TyrIle: 0.647 ± 0.028
0.348TyrLys: 0.348 ± 0.025
2.021TyrLeu: 2.021 ± 0.048
0.298TyrMet: 0.298 ± 0.018
0.462TyrAsn: 0.462 ± 0.027
0.982TyrPro: 0.982 ± 0.036
0.55TyrGln: 0.55 ± 0.028
1.636TyrArg: 1.636 ± 0.05
1.047TyrSer: 1.047 ± 0.036
1.097TyrThr: 1.097 ± 0.045
1.627TyrVal: 1.627 ± 0.044
0.257TyrTrp: 0.257 ± 0.018
0.436TyrTyr: 0.436 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2672 proteins (793030 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski