Amino acid dipepetide frequency for Caenorhabditis elegans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.796AlaAla: 5.796 ± 0.047	1.113AlaCys: 1.113 ± 0.013	3.294AlaAsp: 3.294 ± 0.025	4.298AlaGlu: 4.298 ± 0.038	2.587AlaPhe: 2.587 ± 0.02	3.454AlaGly: 3.454 ± 0.027	1.462AlaHis: 1.462 ± 0.012	3.793AlaIle: 3.793 ± 0.021	3.831AlaLys: 3.831 ± 0.032	5.252AlaLeu: 5.252 ± 0.033	1.637AlaMet: 1.637 ± 0.012	2.764AlaAsn: 2.764 ± 0.017	3.948AlaPro: 3.948 ± 0.04	2.888AlaGln: 2.888 ± 0.025	3.195AlaArg: 3.195 ± 0.022	5.249AlaSer: 5.249 ± 0.031	4.042AlaThr: 4.042 ± 0.026	4.32AlaVal: 4.32 ± 0.027	0.573AlaTrp: 0.573 ± 0.008	1.693AlaTyr: 1.693 ± 0.014	0.0AlaXaa: 0.0 ± 0.0
Cys	1.201CysAla: 1.201 ± 0.015	0.534CysCys: 0.534 ± 0.012	1.109CysAsp: 1.109 ± 0.014	1.211CysGlu: 1.211 ± 0.014	0.885CysPhe: 0.885 ± 0.011	1.224CysGly: 1.224 ± 0.014	0.477CysHis: 0.477 ± 0.007	1.123CysIle: 1.123 ± 0.011	1.047CysLys: 1.047 ± 0.012	1.619CysLeu: 1.619 ± 0.014	0.463CysMet: 0.463 ± 0.006	0.856CysAsn: 0.856 ± 0.012	1.034CysPro: 1.034 ± 0.019	0.874CysGln: 0.874 ± 0.017	1.052CysArg: 1.052 ± 0.012	1.687CysSer: 1.687 ± 0.02	1.088CysThr: 1.088 ± 0.012	1.237CysVal: 1.237 ± 0.015	0.212CysTrp: 0.212 ± 0.005	0.581CysTyr: 0.581 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	3.509AspAla: 3.509 ± 0.034	0.962AspCys: 0.962 ± 0.013	4.244AspAsp: 4.244 ± 0.033	4.746AspGlu: 4.746 ± 0.033	2.44AspPhe: 2.44 ± 0.014	3.603AspGly: 3.603 ± 0.052	1.19AspHis: 1.19 ± 0.012	2.989AspIle: 2.989 ± 0.019	2.909AspLys: 2.909 ± 0.03	4.372AspLeu: 4.372 ± 0.026	1.3AspMet: 1.3 ± 0.011	2.252AspAsn: 2.252 ± 0.014	2.434AspPro: 2.434 ± 0.018	2.056AspGln: 2.056 ± 0.017	2.691AspArg: 2.691 ± 0.023	4.112AspSer: 4.112 ± 0.026	2.597AspThr: 2.597 ± 0.014	3.683AspVal: 3.683 ± 0.026	0.619AspTrp: 0.619 ± 0.007	1.728AspTyr: 1.728 ± 0.014	0.0AspXaa: 0.0 ± 0.0
Glu	4.239GluAla: 4.239 ± 0.04	1.158GluCys: 1.158 ± 0.016	4.045GluAsp: 4.045 ± 0.028	6.365GluGlu: 6.365 ± 0.053	2.453GluPhe: 2.453 ± 0.017	2.773GluGly: 2.773 ± 0.024	1.621GluHis: 1.621 ± 0.013	4.076GluIle: 4.076 ± 0.025	5.937GluLys: 5.937 ± 0.049	5.364GluLeu: 5.364 ± 0.033	1.965GluMet: 1.965 ± 0.015	3.706GluAsn: 3.706 ± 0.017	2.827GluPro: 2.827 ± 0.034	2.962GluGln: 2.962 ± 0.024	3.559GluArg: 3.559 ± 0.03	4.528GluSer: 4.528 ± 0.033	3.9GluThr: 3.9 ± 0.038	3.709GluVal: 3.709 ± 0.031	0.722GluTrp: 0.722 ± 0.008	1.928GluTyr: 1.928 ± 0.013	0.0GluXaa: 0.0 ± 0.0
Phe	2.691PheAla: 2.691 ± 0.019	0.987PheCys: 0.987 ± 0.011	2.605PheAsp: 2.605 ± 0.016	2.756PheGlu: 2.756 ± 0.019	2.261PhePhe: 2.261 ± 0.019	2.869PheGly: 2.869 ± 0.021	1.055PheHis: 1.055 ± 0.009	2.58PheIle: 2.58 ± 0.018	2.156PheLys: 2.156 ± 0.016	4.084PheLeu: 4.084 ± 0.023	1.118PheMet: 1.118 ± 0.01	2.053PheAsn: 2.053 ± 0.014	1.804PhePro: 1.804 ± 0.015	1.701PheGln: 1.701 ± 0.015	2.086PheArg: 2.086 ± 0.014	3.496PheSer: 3.496 ± 0.021	2.335PheThr: 2.335 ± 0.015	2.984PheVal: 2.984 ± 0.018	0.531PheTrp: 0.531 ± 0.007	1.576PheTyr: 1.576 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	3.603GlyAla: 3.603 ± 0.028	1.056GlyCys: 1.056 ± 0.013	2.907GlyAsp: 2.907 ± 0.021	3.34GlyGlu: 3.34 ± 0.031	2.492GlyPhe: 2.492 ± 0.02	4.459GlyGly: 4.459 ± 0.049	1.239GlyHis: 1.239 ± 0.011	3.04GlyIle: 3.04 ± 0.017	3.32GlyLys: 3.32 ± 0.026	3.909GlyLeu: 3.909 ± 0.024	1.314GlyMet: 1.314 ± 0.013	2.715GlyAsn: 2.715 ± 0.025	2.352GlyPro: 2.352 ± 0.039	2.151GlyGln: 2.151 ± 0.023	2.744GlyArg: 2.744 ± 0.02	4.665GlySer: 4.665 ± 0.033	3.148GlyThr: 3.148 ± 0.025	3.278GlyVal: 3.278 ± 0.019	0.637GlyTrp: 0.637 ± 0.01	1.948GlyTyr: 1.948 ± 0.018	0.0GlyXaa: 0.0 ± 0.0
His	1.253HisAla: 1.253 ± 0.011	0.5HisCys: 0.5 ± 0.007	1.139HisAsp: 1.139 ± 0.01	1.345HisGlu: 1.345 ± 0.011	1.194HisPhe: 1.194 ± 0.011	1.308HisGly: 1.308 ± 0.014	0.99HisHis: 0.99 ± 0.016	1.283HisIle: 1.283 ± 0.012	1.135HisLys: 1.135 ± 0.009	2.135HisLeu: 2.135 ± 0.017	0.569HisMet: 0.569 ± 0.007	1.046HisAsn: 1.046 ± 0.01	1.221HisPro: 1.221 ± 0.012	1.184HisGln: 1.184 ± 0.014	1.397HisArg: 1.397 ± 0.012	1.816HisSer: 1.816 ± 0.012	1.143HisThr: 1.143 ± 0.01	1.571HisVal: 1.571 ± 0.012	0.277HisTrp: 0.277 ± 0.005	0.797HisTyr: 0.797 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.862IleAla: 3.862 ± 0.022	1.265IleCys: 1.265 ± 0.013	3.495IleAsp: 3.495 ± 0.023	3.898IleGlu: 3.898 ± 0.02	2.899IlePhe: 2.899 ± 0.023	3.251IleGly: 3.251 ± 0.022	1.352IleHis: 1.352 ± 0.01	3.465IleIle: 3.465 ± 0.021	2.966IleLys: 2.966 ± 0.019	5.157IleLeu: 5.157 ± 0.027	1.329IleMet: 1.329 ± 0.013	2.565IleAsn: 2.565 ± 0.016	3.159IlePro: 3.159 ± 0.022	2.315IleGln: 2.315 ± 0.013	3.143IleArg: 3.143 ± 0.019	4.786IleSer: 4.786 ± 0.024	3.209IleThr: 3.209 ± 0.018	3.974IleVal: 3.974 ± 0.022	0.632IleTrp: 0.632 ± 0.008	1.818IleTyr: 1.818 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	3.454LysAla: 3.454 ± 0.026	1.286LysCys: 1.286 ± 0.016	3.234LysAsp: 3.234 ± 0.039	4.538LysGlu: 4.538 ± 0.044	2.438LysPhe: 2.438 ± 0.016	2.414LysGly: 2.414 ± 0.019	1.328LysHis: 1.328 ± 0.012	3.772LysIle: 3.772 ± 0.021	5.813LysLys: 5.813 ± 0.063	5.417LysLeu: 5.417 ± 0.051	1.87LysMet: 1.87 ± 0.013	3.306LysAsn: 3.306 ± 0.018	2.844LysPro: 2.844 ± 0.031	2.42LysGln: 2.42 ± 0.029	3.519LysArg: 3.519 ± 0.02	4.722LysSer: 4.722 ± 0.032	3.674LysThr: 3.674 ± 0.022	3.432LysVal: 3.432 ± 0.021	0.748LysTrp: 0.748 ± 0.009	1.934LysTyr: 1.934 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	5.595LeuAla: 5.595 ± 0.028	1.585LeuCys: 1.585 ± 0.014	4.366LeuAsp: 4.366 ± 0.026	5.647LeuGlu: 5.647 ± 0.034	3.868LeuPhe: 3.868 ± 0.025	3.885LeuGly: 3.885 ± 0.02	2.02LeuHis: 2.02 ± 0.015	5.05LeuIle: 5.05 ± 0.029	5.563LeuLys: 5.563 ± 0.039	7.919LeuLeu: 7.919 ± 0.043	2.147LeuMet: 2.147 ± 0.017	4.044LeuAsn: 4.044 ± 0.024	4.355LeuPro: 4.355 ± 0.038	3.615LeuGln: 3.615 ± 0.026	4.598LeuArg: 4.598 ± 0.027	6.54LeuSer: 6.54 ± 0.028	4.74LeuThr: 4.74 ± 0.021	4.999LeuVal: 4.999 ± 0.02	0.807LeuTrp: 0.807 ± 0.009	2.299LeuTyr: 2.299 ± 0.017	0.0LeuXaa: 0.0 ± 0.0
Met	1.765MetAla: 1.765 ± 0.013	0.507MetCys: 0.507 ± 0.007	1.421MetAsp: 1.421 ± 0.011	1.769MetGlu: 1.769 ± 0.013	1.15MetPhe: 1.15 ± 0.011	1.183MetGly: 1.183 ± 0.011	0.556MetHis: 0.556 ± 0.006	1.551MetIle: 1.551 ± 0.012	1.744MetLys: 1.744 ± 0.013	2.154MetLeu: 2.154 ± 0.017	0.883MetMet: 0.883 ± 0.01	1.355MetAsn: 1.355 ± 0.011	1.197MetPro: 1.197 ± 0.012	1.046MetGln: 1.046 ± 0.012	1.41MetArg: 1.41 ± 0.011	2.268MetSer: 2.268 ± 0.016	1.553MetThr: 1.553 ± 0.012	1.424MetVal: 1.424 ± 0.011	0.256MetTrp: 0.256 ± 0.004	0.741MetTyr: 0.741 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.928AsnAla: 2.928 ± 0.016	0.958AsnCys: 0.958 ± 0.011	2.616AsnAsp: 2.616 ± 0.015	3.147AsnGlu: 3.147 ± 0.016	2.129AsnPhe: 2.129 ± 0.015	3.454AsnGly: 3.454 ± 0.025	1.097AsnHis: 1.097 ± 0.01	2.697AsnIle: 2.697 ± 0.016	2.422AsnLys: 2.422 ± 0.018	3.993AsnLeu: 3.993 ± 0.025	1.247AsnMet: 1.247 ± 0.011	2.511AsnAsn: 2.511 ± 0.019	2.25AsnPro: 2.25 ± 0.015	2.104AsnGln: 2.104 ± 0.017	2.499AsnArg: 2.499 ± 0.015	3.989AsnSer: 3.989 ± 0.022	2.446AsnThr: 2.446 ± 0.016	3.152AsnVal: 3.152 ± 0.017	0.523AsnTrp: 0.523 ± 0.007	1.621AsnTyr: 1.621 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	3.588ProAla: 3.588 ± 0.034	0.713ProCys: 0.713 ± 0.01	2.399ProAsp: 2.399 ± 0.029	3.304ProGlu: 3.304 ± 0.028	1.932ProPhe: 1.932 ± 0.014	2.796ProGly: 2.796 ± 0.045	1.01ProHis: 1.01 ± 0.01	2.962ProIle: 2.962 ± 0.024	2.917ProLys: 2.917 ± 0.024	3.776ProLeu: 3.776 ± 0.033	1.266ProMet: 1.266 ± 0.013	2.29ProAsn: 2.29 ± 0.017	4.442ProPro: 4.442 ± 0.045	2.266ProGln: 2.266 ± 0.021	2.404ProArg: 2.404 ± 0.017	4.734ProSer: 4.734 ± 0.034	3.73ProThr: 3.73 ± 0.048	3.359ProVal: 3.359 ± 0.033	0.394ProTrp: 0.394 ± 0.006	1.359ProTyr: 1.359 ± 0.014	0.0ProXaa: 0.0 ± 0.0
Gln	2.568GlnAla: 2.568 ± 0.025	0.899GlnCys: 0.899 ± 0.018	1.664GlnAsp: 1.664 ± 0.013	2.508GlnGlu: 2.508 ± 0.028	1.866GlnPhe: 1.866 ± 0.014	1.766GlnGly: 1.766 ± 0.018	1.149GlnHis: 1.149 ± 0.013	2.456GlnIle: 2.456 ± 0.015	2.99GlnLys: 2.99 ± 0.028	4.045GlnLeu: 4.045 ± 0.027	1.392GlnMet: 1.392 ± 0.014	2.297GlnAsn: 2.297 ± 0.021	2.286GlnPro: 2.286 ± 0.024	3.561GlnGln: 3.561 ± 0.058	2.314GlnArg: 2.314 ± 0.016	3.009GlnSer: 3.009 ± 0.018	2.266GlnThr: 2.266 ± 0.016	2.309GlnVal: 2.309 ± 0.015	0.5GlnTrp: 0.5 ± 0.007	1.346GlnTyr: 1.346 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	3.106ArgAla: 3.106 ± 0.018	0.972ArgCys: 0.972 ± 0.012	2.715ArgAsp: 2.715 ± 0.018	3.424ArgGlu: 3.424 ± 0.029	2.201ArgPhe: 2.201 ± 0.016	2.585ArgGly: 2.585 ± 0.022	1.364ArgHis: 1.364 ± 0.013	3.11ArgIle: 3.11 ± 0.019	3.873ArgLys: 3.873 ± 0.023	4.433ArgLeu: 4.433 ± 0.026	1.372ArgMet: 1.372 ± 0.01	2.744ArgAsn: 2.744 ± 0.017	2.417ArgPro: 2.417 ± 0.02	2.371ArgGln: 2.371 ± 0.018	4.146ArgArg: 4.146 ± 0.033	4.121ArgSer: 4.121 ± 0.027	2.729ArgThr: 2.729 ± 0.015	3.052ArgVal: 3.052 ± 0.018	0.554ArgTrp: 0.554 ± 0.007	1.531ArgTyr: 1.531 ± 0.014	0.0ArgXaa: 0.0 ± 0.0
Ser	5.467SerAla: 5.467 ± 0.025	1.523SerCys: 1.523 ± 0.02	4.361SerAsp: 4.361 ± 0.025	5.048SerGlu: 5.048 ± 0.035	3.396SerPhe: 3.396 ± 0.018	4.7SerGly: 4.7 ± 0.048	1.731SerHis: 1.731 ± 0.013	4.711SerIle: 4.711 ± 0.024	4.433SerLys: 4.433 ± 0.028	6.398SerLeu: 6.398 ± 0.029	2.019SerMet: 2.019 ± 0.015	3.83SerAsn: 3.83 ± 0.022	4.369SerPro: 4.369 ± 0.033	3.251SerGln: 3.251 ± 0.02	4.155SerArg: 4.155 ± 0.026	9.568SerSer: 9.568 ± 0.073	6.032SerThr: 6.032 ± 0.046	4.862SerVal: 4.862 ± 0.026	0.778SerTrp: 0.778 ± 0.008	2.182SerTyr: 2.182 ± 0.017	0.0SerXaa: 0.0 ± 0.0
Thr	3.983ThrAla: 3.983 ± 0.025	1.285ThrCys: 1.285 ± 0.018	2.946ThrAsp: 2.946 ± 0.046	3.613ThrGlu: 3.613 ± 0.036	2.491ThrPhe: 2.491 ± 0.018	3.163ThrGly: 3.163 ± 0.022	1.15ThrHis: 1.15 ± 0.01	3.74ThrIle: 3.74 ± 0.023	3.065ThrLys: 3.065 ± 0.019	4.676ThrLeu: 4.676 ± 0.022	1.409ThrMet: 1.409 ± 0.012	2.583ThrAsn: 2.583 ± 0.016	3.665ThrPro: 3.665 ± 0.031	2.062ThrGln: 2.062 ± 0.016	2.629ThrArg: 2.629 ± 0.015	5.679ThrSer: 5.679 ± 0.04	5.329ThrThr: 5.329 ± 0.084	4.265ThrVal: 4.265 ± 0.033	0.633ThrTrp: 0.633 ± 0.007	1.682ThrTyr: 1.682 ± 0.015	0.0ThrXaa: 0.0 ± 0.0
Val	4.274ValAla: 4.274 ± 0.017	1.278ValCys: 1.278 ± 0.014	3.577ValAsp: 3.577 ± 0.018	4.458ValGlu: 4.458 ± 0.054	2.933ValPhe: 2.933 ± 0.017	3.083ValGly: 3.083 ± 0.018	1.471ValHis: 1.471 ± 0.012	3.743ValIle: 3.743 ± 0.024	3.654ValLys: 3.654 ± 0.023	5.343ValLeu: 5.343 ± 0.025	1.49ValMet: 1.49 ± 0.013	2.768ValAsn: 2.768 ± 0.017	3.266ValPro: 3.266 ± 0.028	2.587ValGln: 2.587 ± 0.016	3.001ValArg: 3.001 ± 0.019	4.687ValSer: 4.687 ± 0.02	3.768ValThr: 3.768 ± 0.03	4.347ValVal: 4.347 ± 0.025	0.647ValTrp: 0.647 ± 0.007	1.883ValTyr: 1.883 ± 0.013	0.0ValXaa: 0.0 ± 0.0
Trp	0.593TrpAla: 0.593 ± 0.008	0.216TrpCys: 0.216 ± 0.005	0.543TrpAsp: 0.543 ± 0.008	0.558TrpGlu: 0.558 ± 0.007	0.53TrpPhe: 0.53 ± 0.007	0.443TrpGly: 0.443 ± 0.008	0.245TrpHis: 0.245 ± 0.004	0.729TrpIle: 0.729 ± 0.009	0.837TrpLys: 0.837 ± 0.009	0.978TrpLeu: 0.978 ± 0.009	0.356TrpMet: 0.356 ± 0.005	0.634TrpAsn: 0.634 ± 0.009	0.393TrpPro: 0.393 ± 0.007	0.427TrpGln: 0.427 ± 0.007	0.59TrpArg: 0.59 ± 0.007	0.793TrpSer: 0.793 ± 0.009	0.677TrpThr: 0.677 ± 0.009	0.535TrpVal: 0.535 ± 0.008	0.161TrpTrp: 0.161 ± 0.004	0.377TrpTyr: 0.377 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.714TyrAla: 1.714 ± 0.015	0.719TyrCys: 0.719 ± 0.009	1.754TyrAsp: 1.754 ± 0.013	1.866TyrGlu: 1.866 ± 0.013	1.582TyrPhe: 1.582 ± 0.015	1.902TyrGly: 1.902 ± 0.016	0.793TyrHis: 0.793 ± 0.008	1.672TyrIle: 1.672 ± 0.016	1.553TyrLys: 1.553 ± 0.014	2.619TyrLeu: 2.619 ± 0.018	0.786TyrMet: 0.786 ± 0.008	1.461TyrAsn: 1.461 ± 0.013	1.377TyrPro: 1.377 ± 0.021	1.351TyrGln: 1.351 ± 0.015	1.687TyrArg: 1.687 ± 0.013	2.363TyrSer: 2.363 ± 0.016	1.645TyrThr: 1.645 ± 0.012	1.767TyrVal: 1.767 ± 0.014	0.412TyrTrp: 0.412 ± 0.006	1.198TyrTyr: 1.198 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 28314 proteins (13466555 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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