Amino acid dipepetide frequency for Streptomyces tsukubensis (strain DSM 42081 / NBRC 108919 / NRRL 18488 / 9993)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.111AlaAla: 23.111 ± 0.159	1.033AlaCys: 1.033 ± 0.023	8.42AlaAsp: 8.42 ± 0.07	9.026AlaGlu: 9.026 ± 0.089	3.524AlaPhe: 3.524 ± 0.049	14.219AlaGly: 14.219 ± 0.124	2.709AlaHis: 2.709 ± 0.042	3.199AlaIle: 3.199 ± 0.044	2.555AlaLys: 2.555 ± 0.048	14.947AlaLeu: 14.947 ± 0.111	2.255AlaMet: 2.255 ± 0.033	1.787AlaAsn: 1.787 ± 0.036	8.083AlaPro: 8.083 ± 0.093	3.337AlaGln: 3.337 ± 0.04	10.879AlaArg: 10.879 ± 0.094	5.467AlaSer: 5.467 ± 0.053	6.716AlaThr: 6.716 ± 0.067	13.109AlaVal: 13.109 ± 0.107	1.744AlaTrp: 1.744 ± 0.03	2.678AlaTyr: 2.678 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.04CysAla: 1.04 ± 0.023	0.095CysCys: 0.095 ± 0.007	0.47CysAsp: 0.47 ± 0.015	0.409CysGlu: 0.409 ± 0.014	0.203CysPhe: 0.203 ± 0.01	0.94CysGly: 0.94 ± 0.023	0.187CysHis: 0.187 ± 0.008	0.18CysIle: 0.18 ± 0.009	0.115CysLys: 0.115 ± 0.007	0.673CysLeu: 0.673 ± 0.018	0.113CysMet: 0.113 ± 0.008	0.123CysAsn: 0.123 ± 0.007	0.52CysPro: 0.52 ± 0.018	0.142CysGln: 0.142 ± 0.008	0.65CysArg: 0.65 ± 0.019	0.429CysSer: 0.429 ± 0.015	0.506CysThr: 0.506 ± 0.017	0.569CysVal: 0.569 ± 0.019	0.122CysTrp: 0.122 ± 0.007	0.139CysTyr: 0.139 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.447AspAla: 7.447 ± 0.075	0.429AspCys: 0.429 ± 0.016	3.545AspAsp: 3.545 ± 0.052	3.785AspGlu: 3.785 ± 0.046	1.526AspPhe: 1.526 ± 0.032	6.828AspGly: 6.828 ± 0.07	1.388AspHis: 1.388 ± 0.024	1.848AspIle: 1.848 ± 0.029	0.932AspLys: 0.932 ± 0.026	5.923AspLeu: 5.923 ± 0.062	0.71AspMet: 0.71 ± 0.021	0.845AspAsn: 0.845 ± 0.023	4.99AspPro: 4.99 ± 0.049	1.389AspGln: 1.389 ± 0.026	5.534AspArg: 5.534 ± 0.054	2.457AspSer: 2.457 ± 0.032	3.348AspThr: 3.348 ± 0.044	4.217AspVal: 4.217 ± 0.046	1.029AspTrp: 1.029 ± 0.026	1.079AspTyr: 1.079 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.269GluAla: 7.269 ± 0.087	0.381GluCys: 0.381 ± 0.015	2.635GluAsp: 2.635 ± 0.037	3.45GluGlu: 3.45 ± 0.045	1.376GluPhe: 1.376 ± 0.025	4.413GluGly: 4.413 ± 0.048	1.441GluHis: 1.441 ± 0.025	2.269GluIle: 2.269 ± 0.035	1.403GluLys: 1.403 ± 0.031	7.006GluLeu: 7.006 ± 0.063	0.801GluMet: 0.801 ± 0.02	1.025GluAsn: 1.025 ± 0.023	3.618GluPro: 3.618 ± 0.043	2.14GluGln: 2.14 ± 0.031	5.601GluArg: 5.601 ± 0.06	2.637GluSer: 2.637 ± 0.039	3.079GluThr: 3.079 ± 0.043	4.201GluVal: 4.201 ± 0.053	0.784GluTrp: 0.784 ± 0.019	1.104GluTyr: 1.104 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.564PheAla: 3.564 ± 0.042	0.254PheCys: 0.254 ± 0.012	1.941PheAsp: 1.941 ± 0.032	1.333PheGlu: 1.333 ± 0.026	0.85PhePhe: 0.85 ± 0.02	2.881PheGly: 2.881 ± 0.042	0.591PheHis: 0.591 ± 0.015	0.666PheIle: 0.666 ± 0.017	0.425PheLys: 0.425 ± 0.016	2.454PheLeu: 2.454 ± 0.037	0.353PheMet: 0.353 ± 0.014	0.511PheAsn: 0.511 ± 0.016	1.377PhePro: 1.377 ± 0.026	0.602PheGln: 0.602 ± 0.017	1.928PheArg: 1.928 ± 0.038	1.403PheSer: 1.403 ± 0.029	2.028PheThr: 2.028 ± 0.033	2.106PheVal: 2.106 ± 0.037	0.393PheTrp: 0.393 ± 0.015	0.524PheTyr: 0.524 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	12.033GlyAla: 12.033 ± 0.101	0.827GlyCys: 0.827 ± 0.022	5.414GlyAsp: 5.414 ± 0.051	5.34GlyGlu: 5.34 ± 0.063	2.853GlyPhe: 2.853 ± 0.036	9.749GlyGly: 9.749 ± 0.116	2.359GlyHis: 2.359 ± 0.036	3.552GlyIle: 3.552 ± 0.043	2.269GlyLys: 2.269 ± 0.045	9.81GlyLeu: 9.81 ± 0.083	1.888GlyMet: 1.888 ± 0.032	1.774GlyAsn: 1.774 ± 0.039	6.45GlyPro: 6.45 ± 0.073	2.451GlyGln: 2.451 ± 0.036	8.623GlyArg: 8.623 ± 0.07	5.597GlySer: 5.597 ± 0.056	7.197GlyThr: 7.197 ± 0.082	7.367GlyVal: 7.367 ± 0.093	1.624GlyTrp: 1.624 ± 0.027	2.248GlyTyr: 2.248 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.482HisAla: 2.482 ± 0.038	0.206HisCys: 0.206 ± 0.011	1.267HisAsp: 1.267 ± 0.029	1.122HisGlu: 1.122 ± 0.022	0.596HisPhe: 0.596 ± 0.017	2.51HisGly: 2.51 ± 0.038	0.752HisHis: 0.752 ± 0.025	0.682HisIle: 0.682 ± 0.02	0.286HisLys: 0.286 ± 0.014	2.303HisLeu: 2.303 ± 0.032	0.309HisMet: 0.309 ± 0.012	0.327HisAsn: 0.327 ± 0.012	1.899HisPro: 1.899 ± 0.031	0.652HisGln: 0.652 ± 0.019	2.305HisArg: 2.305 ± 0.038	1.033HisSer: 1.033 ± 0.025	1.358HisThr: 1.358 ± 0.032	1.386HisVal: 1.386 ± 0.03	0.365HisTrp: 0.365 ± 0.013	0.461HisTyr: 0.461 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.896IleAla: 4.896 ± 0.053	0.276IleCys: 0.276 ± 0.012	2.146IleAsp: 2.146 ± 0.031	1.64IleGlu: 1.64 ± 0.03	0.617IlePhe: 0.617 ± 0.019	3.582IleGly: 3.582 ± 0.046	0.599IleHis: 0.599 ± 0.018	0.793IleIle: 0.793 ± 0.023	0.596IleLys: 0.596 ± 0.019	2.224IleLeu: 2.224 ± 0.033	0.411IleMet: 0.411 ± 0.016	0.669IleAsn: 0.669 ± 0.02	1.785IlePro: 1.785 ± 0.03	0.613IleGln: 0.613 ± 0.016	2.504IleArg: 2.504 ± 0.032	1.647IleSer: 1.647 ± 0.03	2.269IleThr: 2.269 ± 0.035	2.692IleVal: 2.692 ± 0.044	0.362IleTrp: 0.362 ± 0.013	0.464IleTyr: 0.464 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.562LysAla: 2.562 ± 0.045	0.098LysCys: 0.098 ± 0.007	1.173LysAsp: 1.173 ± 0.027	1.135LysGlu: 1.135 ± 0.028	0.418LysPhe: 0.418 ± 0.016	1.727LysGly: 1.727 ± 0.039	0.36LysHis: 0.36 ± 0.013	0.796LysIle: 0.796 ± 0.024	0.792LysLys: 0.792 ± 0.028	1.965LysLeu: 1.965 ± 0.038	0.329LysMet: 0.329 ± 0.012	0.491LysAsn: 0.491 ± 0.02	1.281LysPro: 1.281 ± 0.031	0.582LysGln: 0.582 ± 0.019	1.282LysArg: 1.282 ± 0.029	1.014LysSer: 1.014 ± 0.025	1.261LysThr: 1.261 ± 0.032	1.625LysVal: 1.625 ± 0.035	0.23LysTrp: 0.23 ± 0.009	0.389LysTyr: 0.389 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.044LeuAla: 15.044 ± 0.108	0.787LeuCys: 0.787 ± 0.023	6.646LeuAsp: 6.646 ± 0.065	4.619LeuGlu: 4.619 ± 0.056	2.494LeuPhe: 2.494 ± 0.035	9.166LeuGly: 9.166 ± 0.073	2.305LeuHis: 2.305 ± 0.035	3.201LeuIle: 3.201 ± 0.048	1.928LeuLys: 1.928 ± 0.037	11.388LeuLeu: 11.388 ± 0.101	1.558LeuMet: 1.558 ± 0.026	1.617LeuAsn: 1.617 ± 0.028	6.845LeuPro: 6.845 ± 0.076	1.979LeuGln: 1.979 ± 0.035	8.899LeuArg: 8.899 ± 0.074	5.22LeuSer: 5.22 ± 0.05	6.982LeuThr: 6.982 ± 0.059	8.626LeuVal: 8.626 ± 0.074	1.32LeuTrp: 1.32 ± 0.029	1.799LeuTyr: 1.799 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.093MetAla: 2.093 ± 0.027	0.116MetCys: 0.116 ± 0.007	0.842MetAsp: 0.842 ± 0.02	0.698MetGlu: 0.698 ± 0.018	0.423MetPhe: 0.423 ± 0.015	1.209MetGly: 1.209 ± 0.025	0.31MetHis: 0.31 ± 0.012	0.628MetIle: 0.628 ± 0.016	0.393MetLys: 0.393 ± 0.015	1.576MetLeu: 1.576 ± 0.03	0.251MetMet: 0.251 ± 0.011	0.409MetAsn: 0.409 ± 0.014	1.043MetPro: 1.043 ± 0.022	0.383MetGln: 0.383 ± 0.015	1.342MetArg: 1.342 ± 0.023	1.16MetSer: 1.16 ± 0.026	1.435MetThr: 1.435 ± 0.029	1.206MetVal: 1.206 ± 0.027	0.188MetTrp: 0.188 ± 0.011	0.315MetTyr: 0.315 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.095AsnAla: 2.095 ± 0.038	0.147AsnCys: 0.147 ± 0.008	0.883AsnAsp: 0.883 ± 0.021	0.791AsnGlu: 0.791 ± 0.018	0.413AsnPhe: 0.413 ± 0.015	1.914AsnGly: 1.914 ± 0.044	0.361AsnHis: 0.361 ± 0.012	0.611AsnIle: 0.611 ± 0.017	0.393AsnLys: 0.393 ± 0.015	1.466AsnLeu: 1.466 ± 0.03	0.263AsnMet: 0.263 ± 0.01	0.414AsnAsn: 0.414 ± 0.017	1.344AsnPro: 1.344 ± 0.03	0.473AsnGln: 0.473 ± 0.015	1.319AsnArg: 1.319 ± 0.031	0.864AsnSer: 0.864 ± 0.024	1.164AsnThr: 1.164 ± 0.03	1.235AsnVal: 1.235 ± 0.028	0.274AsnTrp: 0.274 ± 0.013	0.361AsnTyr: 0.361 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	10.06ProAla: 10.06 ± 0.095	0.349ProCys: 0.349 ± 0.015	4.835ProAsp: 4.835 ± 0.057	4.774ProGlu: 4.774 ± 0.052	1.507ProPhe: 1.507 ± 0.029	8.328ProGly: 8.328 ± 0.088	1.395ProHis: 1.395 ± 0.029	1.158ProIle: 1.158 ± 0.025	1.134ProLys: 1.134 ± 0.027	5.613ProLeu: 5.613 ± 0.067	0.926ProMet: 0.926 ± 0.022	0.827ProAsn: 0.827 ± 0.022	4.314ProPro: 4.314 ± 0.085	1.649ProGln: 1.649 ± 0.036	4.346ProArg: 4.346 ± 0.051	3.157ProSer: 3.157 ± 0.039	3.02ProThr: 3.02 ± 0.044	6.136ProVal: 6.136 ± 0.059	0.923ProTrp: 0.923 ± 0.024	1.45ProTyr: 1.45 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.223GlnAla: 3.223 ± 0.041	0.165GlnCys: 0.165 ± 0.008	1.202GlnAsp: 1.202 ± 0.028	1.323GlnGlu: 1.323 ± 0.028	0.617GlnPhe: 0.617 ± 0.017	2.142GlnGly: 2.142 ± 0.051	0.591GlnHis: 0.591 ± 0.017	1.003GlnIle: 1.003 ± 0.023	0.548GlnLys: 0.548 ± 0.018	2.808GlnLeu: 2.808 ± 0.037	0.428GlnMet: 0.428 ± 0.016	0.475GlnAsn: 0.475 ± 0.013	1.589GlnPro: 1.589 ± 0.035	1.14GlnGln: 1.14 ± 0.032	2.226GlnArg: 2.226 ± 0.032	1.192GlnSer: 1.192 ± 0.025	1.27GlnThr: 1.27 ± 0.027	2.04GlnVal: 2.04 ± 0.038	0.423GlnTrp: 0.423 ± 0.016	0.568GlnTyr: 0.568 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.534ArgAla: 10.534 ± 0.09	0.621ArgCys: 0.621 ± 0.019	4.553ArgAsp: 4.553 ± 0.051	4.84ArgGlu: 4.84 ± 0.05	2.316ArgPhe: 2.316 ± 0.034	6.256ArgGly: 6.256 ± 0.067	2.087ArgHis: 2.087 ± 0.031	3.789ArgIle: 3.789 ± 0.04	1.578ArgLys: 1.578 ± 0.035	8.844ArgLeu: 8.844 ± 0.077	1.773ArgMet: 1.773 ± 0.031	1.462ArgAsn: 1.462 ± 0.022	5.626ArgPro: 5.626 ± 0.064	2.19ArgGln: 2.19 ± 0.036	8.102ArgArg: 8.102 ± 0.104	4.378ArgSer: 4.378 ± 0.046	6.088ArgThr: 6.088 ± 0.067	5.657ArgVal: 5.657 ± 0.055	1.331ArgTrp: 1.331 ± 0.026	1.822ArgTyr: 1.822 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.756SerAla: 6.756 ± 0.06	0.403SerCys: 0.403 ± 0.013	2.494SerAsp: 2.494 ± 0.036	2.159SerGlu: 2.159 ± 0.032	1.484SerPhe: 1.484 ± 0.027	6.385SerGly: 6.385 ± 0.074	0.977SerHis: 0.977 ± 0.023	1.251SerIle: 1.251 ± 0.027	0.85SerLys: 0.85 ± 0.022	4.636SerLeu: 4.636 ± 0.057	0.963SerMet: 0.963 ± 0.023	0.781SerAsn: 0.781 ± 0.022	3.269SerPro: 3.269 ± 0.043	1.083SerGln: 1.083 ± 0.025	3.728SerArg: 3.728 ± 0.041	2.62SerSer: 2.62 ± 0.043	2.948SerThr: 2.948 ± 0.04	4.276SerVal: 4.276 ± 0.047	0.877SerTrp: 0.877 ± 0.023	1.164SerTyr: 1.164 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.937ThrAla: 9.937 ± 0.075	0.38ThrCys: 0.38 ± 0.014	3.665ThrAsp: 3.665 ± 0.042	3.464ThrGlu: 3.464 ± 0.047	1.503ThrPhe: 1.503 ± 0.029	7.672ThrGly: 7.672 ± 0.078	1.16ThrHis: 1.16 ± 0.029	1.6ThrIle: 1.6 ± 0.029	1.021ThrLys: 1.021 ± 0.026	5.564ThrLeu: 5.564 ± 0.05	0.872ThrMet: 0.872 ± 0.017	0.932ThrAsn: 0.932 ± 0.024	4.298ThrPro: 4.298 ± 0.058	1.11ThrGln: 1.11 ± 0.027	3.91ThrArg: 3.91 ± 0.052	2.877ThrSer: 2.877 ± 0.038	4.016ThrThr: 4.016 ± 0.061	6.449ThrVal: 6.449 ± 0.064	0.824ThrTrp: 0.824 ± 0.021	1.283ThrTyr: 1.283 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.346ValAla: 10.346 ± 0.087	0.732ValCys: 0.732 ± 0.024	4.871ValAsp: 4.871 ± 0.051	4.628ValGlu: 4.628 ± 0.059	2.428ValPhe: 2.428 ± 0.038	6.173ValGly: 6.173 ± 0.066	1.986ValHis: 1.986 ± 0.034	2.605ValIle: 2.605 ± 0.039	1.637ValLys: 1.637 ± 0.034	9.56ValLeu: 9.56 ± 0.085	1.378ValMet: 1.378 ± 0.024	1.553ValAsn: 1.553 ± 0.03	5.706ValPro: 5.706 ± 0.068	1.942ValGln: 1.942 ± 0.033	7.462ValArg: 7.462 ± 0.066	4.143ValSer: 4.143 ± 0.048	5.55ValThr: 5.55 ± 0.051	7.814ValVal: 7.814 ± 0.078	1.103ValTrp: 1.103 ± 0.026	1.538ValTyr: 1.538 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.575TrpAla: 1.575 ± 0.031	0.147TrpCys: 0.147 ± 0.009	0.829TrpAsp: 0.829 ± 0.021	0.807TrpGlu: 0.807 ± 0.023	0.464TrpPhe: 0.464 ± 0.015	1.024TrpGly: 1.024 ± 0.029	0.352TrpHis: 0.352 ± 0.013	0.494TrpIle: 0.494 ± 0.017	0.332TrpLys: 0.332 ± 0.013	1.681TrpLeu: 1.681 ± 0.034	0.232TrpMet: 0.232 ± 0.011	0.388TrpAsn: 0.388 ± 0.012	0.785TrpPro: 0.785 ± 0.02	0.582TrpGln: 0.582 ± 0.017	1.324TrpArg: 1.324 ± 0.025	0.92TrpSer: 0.92 ± 0.026	1.067TrpThr: 1.067 ± 0.021	0.929TrpVal: 0.929 ± 0.023	0.336TrpTrp: 0.336 ± 0.017	0.343TrpTyr: 0.343 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.731TyrAla: 2.731 ± 0.034	0.178TyrCys: 0.178 ± 0.01	1.419TyrAsp: 1.419 ± 0.037	1.292TyrGlu: 1.292 ± 0.025	0.572TyrPhe: 0.572 ± 0.017	2.311TyrGly: 2.311 ± 0.037	0.393TyrHis: 0.393 ± 0.014	0.461TyrIle: 0.461 ± 0.015	0.325TyrLys: 0.325 ± 0.014	1.981TyrLeu: 1.981 ± 0.034	0.205TyrMet: 0.205 ± 0.01	0.373TyrAsn: 0.373 ± 0.016	1.078TyrPro: 1.078 ± 0.023	0.535TyrGln: 0.535 ± 0.016	1.883TyrArg: 1.883 ± 0.037	0.888TyrSer: 0.888 ± 0.022	1.235TyrThr: 1.235 ± 0.026	1.515TyrVal: 1.515 ± 0.033	0.358TyrTrp: 0.358 ± 0.014	0.405TyrTyr: 0.405 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6304 proteins (2206834 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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