Eubacterium sp. CAG:86

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5DVU0|R5DVU0_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:86 OX=1262895 GN=BN798_00451 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 4.68IVTSDD7 pKa = 3.63LLVIEE12 pKa = 4.84FADD15 pKa = 3.68NEE17 pKa = 4.3EE18 pKa = 3.99KK19 pKa = 11.05AKK21 pKa = 10.47IYY23 pKa = 9.92MADD26 pKa = 3.84DD27 pKa = 3.97GQTYY31 pKa = 10.48YY32 pKa = 10.19IGDD35 pKa = 3.83YY36 pKa = 8.3ATPDD40 pKa = 3.44EE41 pKa = 4.99LFIAMMKK48 pKa = 9.79EE49 pKa = 3.39IEE51 pKa = 4.38YY52 pKa = 10.35EE53 pKa = 4.18LKK55 pKa = 10.23TSS57 pKa = 3.36

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5E9D2|R5E9D2_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:86 OX=1262895 GN=BN798_00042 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.78TRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.97VHH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2189

0

2189

714217

29

2119

326.3

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.185 ± 0.049

1.489 ± 0.019

6.347 ± 0.038

6.894 ± 0.058

4.092 ± 0.037

6.763 ± 0.056

1.525 ± 0.02

8.331 ± 0.046

7.643 ± 0.038

7.96 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.131 ± 0.025

5.421 ± 0.044

2.842 ± 0.026

2.708 ± 0.029

3.71 ± 0.04

6.335 ± 0.046

5.391 ± 0.05

7.129 ± 0.04

0.707 ± 0.014

4.394 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski