Amino acid dipepetide frequency for Eubacterium sp. CAG:86

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.138AlaAla: 7.138 ± 0.131	1.099AlaCys: 1.099 ± 0.037	5.192AlaAsp: 5.192 ± 0.091	4.318AlaGlu: 4.318 ± 0.084	2.736AlaPhe: 2.736 ± 0.069	6.065AlaGly: 6.065 ± 0.114	0.937AlaHis: 0.937 ± 0.037	5.105AlaIle: 5.105 ± 0.103	5.059AlaLys: 5.059 ± 0.099	6.028AlaLeu: 6.028 ± 0.097	2.17AlaMet: 2.17 ± 0.058	2.692AlaAsn: 2.692 ± 0.07	1.627AlaPro: 1.627 ± 0.05	2.2AlaGln: 2.2 ± 0.065	2.425AlaArg: 2.425 ± 0.065	4.248AlaSer: 4.248 ± 0.083	2.951AlaThr: 2.951 ± 0.091	6.635AlaVal: 6.635 ± 0.11	0.456AlaTrp: 0.456 ± 0.029	2.764AlaTyr: 2.764 ± 0.064	0.001AlaXaa: 0.001 ± 0.001
Cys	1.0CysAla: 1.0 ± 0.043	0.245CysCys: 0.245 ± 0.019	0.973CysAsp: 0.973 ± 0.032	0.923CysGlu: 0.923 ± 0.036	0.564CysPhe: 0.564 ± 0.025	1.379CysGly: 1.379 ± 0.053	0.287CysHis: 0.287 ± 0.021	1.406CysIle: 1.406 ± 0.053	0.893CysLys: 0.893 ± 0.032	1.009CysLeu: 1.009 ± 0.042	0.463CysMet: 0.463 ± 0.029	0.749CysAsn: 0.749 ± 0.03	0.539CysPro: 0.539 ± 0.031	0.319CysGln: 0.319 ± 0.02	0.62CysArg: 0.62 ± 0.033	0.918CysSer: 0.918 ± 0.039	0.759CysThr: 0.759 ± 0.039	1.164CysVal: 1.164 ± 0.04	0.112CysTrp: 0.112 ± 0.014	0.57CysTyr: 0.57 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	4.426AspAla: 4.426 ± 0.076	0.837AspCys: 0.837 ± 0.04	4.143AspAsp: 4.143 ± 0.076	5.76AspGlu: 5.76 ± 0.09	2.65AspPhe: 2.65 ± 0.068	4.51AspGly: 4.51 ± 0.113	0.617AspHis: 0.617 ± 0.03	6.005AspIle: 6.005 ± 0.084	5.137AspLys: 5.137 ± 0.099	3.793AspLeu: 3.793 ± 0.071	2.268AspMet: 2.268 ± 0.058	3.712AspAsn: 3.712 ± 0.09	1.185AspPro: 1.185 ± 0.045	0.731AspGln: 0.731 ± 0.032	2.145AspArg: 2.145 ± 0.068	3.92AspSer: 3.92 ± 0.09	3.46AspThr: 3.46 ± 0.068	4.464AspVal: 4.464 ± 0.081	0.441AspTrp: 0.441 ± 0.03	3.264AspTyr: 3.264 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	4.647GluAla: 4.647 ± 0.086	0.953GluCys: 0.953 ± 0.036	3.873GluAsp: 3.873 ± 0.077	5.354GluGlu: 5.354 ± 0.121	2.583GluPhe: 2.583 ± 0.055	3.46GluGly: 3.46 ± 0.079	1.267GluHis: 1.267 ± 0.046	5.44GluIle: 5.44 ± 0.107	6.715GluLys: 6.715 ± 0.114	6.288GluLeu: 6.288 ± 0.105	2.004GluMet: 2.004 ± 0.063	4.818GluAsn: 4.818 ± 0.091	1.584GluPro: 1.584 ± 0.049	2.37GluGln: 2.37 ± 0.066	2.659GluArg: 2.659 ± 0.064	3.654GluSer: 3.654 ± 0.082	3.317GluThr: 3.317 ± 0.062	4.013GluVal: 4.013 ± 0.091	0.542GluTrp: 0.542 ± 0.026	3.397GluTyr: 3.397 ± 0.078	0.0GluXaa: 0.0 ± 0.0
Phe	3.079PheAla: 3.079 ± 0.07	0.671PheCys: 0.671 ± 0.03	2.908PheAsp: 2.908 ± 0.068	2.669PheGlu: 2.669 ± 0.062	1.633PhePhe: 1.633 ± 0.059	2.996PheGly: 2.996 ± 0.069	0.603PheHis: 0.603 ± 0.029	3.475PheIle: 3.475 ± 0.081	2.691PheLys: 2.691 ± 0.061	3.253PheLeu: 3.253 ± 0.073	1.29PheMet: 1.29 ± 0.045	2.102PheAsn: 2.102 ± 0.065	1.131PhePro: 1.131 ± 0.044	0.774PheGln: 0.774 ± 0.031	1.334PheArg: 1.334 ± 0.047	2.774PheSer: 2.774 ± 0.062	2.461PheThr: 2.461 ± 0.062	3.041PheVal: 3.041 ± 0.081	0.283PheTrp: 0.283 ± 0.02	1.757PheTyr: 1.757 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	4.506GlyAla: 4.506 ± 0.094	1.229GlyCys: 1.229 ± 0.046	3.654GlyAsp: 3.654 ± 0.079	4.065GlyGlu: 4.065 ± 0.081	3.145GlyPhe: 3.145 ± 0.072	4.417GlyGly: 4.417 ± 0.106	1.22GlyHis: 1.22 ± 0.041	6.574GlyIle: 6.574 ± 0.101	5.273GlyLys: 5.273 ± 0.086	5.039GlyLeu: 5.039 ± 0.091	2.38GlyMet: 2.38 ± 0.061	3.661GlyAsn: 3.661 ± 0.082	1.15GlyPro: 1.15 ± 0.042	1.763GlyGln: 1.763 ± 0.05	2.795GlyArg: 2.795 ± 0.075	4.135GlySer: 4.135 ± 0.084	3.962GlyThr: 3.962 ± 0.091	4.861GlyVal: 4.861 ± 0.079	0.553GlyTrp: 0.553 ± 0.03	3.247GlyTyr: 3.247 ± 0.072	0.001GlyXaa: 0.001 ± 0.002
His	0.895HisAla: 0.895 ± 0.033	0.269HisCys: 0.269 ± 0.02	0.976HisAsp: 0.976 ± 0.038	1.031HisGlu: 1.031 ± 0.044	0.743HisPhe: 0.743 ± 0.034	1.08HisGly: 1.08 ± 0.04	0.298HisHis: 0.298 ± 0.031	1.371HisIle: 1.371 ± 0.045	0.981HisLys: 0.981 ± 0.039	1.183HisLeu: 1.183 ± 0.043	0.465HisMet: 0.465 ± 0.025	0.854HisAsn: 0.854 ± 0.038	0.64HisPro: 0.64 ± 0.033	0.386HisGln: 0.386 ± 0.023	0.641HisArg: 0.641 ± 0.028	0.92HisSer: 0.92 ± 0.037	0.749HisThr: 0.749 ± 0.033	1.089HisVal: 1.089 ± 0.043	0.097HisTrp: 0.097 ± 0.011	0.58HisTyr: 0.58 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.177IleAla: 6.177 ± 0.098	1.428IleCys: 1.428 ± 0.052	5.2IleAsp: 5.2 ± 0.089	5.35IleGlu: 5.35 ± 0.097	3.444IlePhe: 3.444 ± 0.081	5.214IleGly: 5.214 ± 0.093	1.218IleHis: 1.218 ± 0.043	7.253IleIle: 7.253 ± 0.138	6.105IleLys: 6.105 ± 0.099	6.687IleLeu: 6.687 ± 0.125	2.482IleMet: 2.482 ± 0.06	4.492IleAsn: 4.492 ± 0.081	2.902IlePro: 2.902 ± 0.058	1.992IleGln: 1.992 ± 0.056	3.135IleArg: 3.135 ± 0.074	5.839IleSer: 5.839 ± 0.086	4.878IleThr: 4.878 ± 0.098	5.591IleVal: 5.591 ± 0.104	0.526IleTrp: 0.526 ± 0.028	3.395IleTyr: 3.395 ± 0.072	0.0IleXaa: 0.0 ± 0.0
Lys	5.186LysAla: 5.186 ± 0.09	0.949LysCys: 0.949 ± 0.042	4.857LysAsp: 4.857 ± 0.09	6.422LysGlu: 6.422 ± 0.107	2.39LysPhe: 2.39 ± 0.058	4.198LysGly: 4.198 ± 0.081	1.115LysHis: 1.115 ± 0.039	6.002LysIle: 6.002 ± 0.101	7.389LysLys: 7.389 ± 0.111	5.82LysLeu: 5.82 ± 0.095	2.481LysMet: 2.481 ± 0.057	5.071LysAsn: 5.071 ± 0.093	2.089LysPro: 2.089 ± 0.064	2.341LysGln: 2.341 ± 0.064	2.925LysArg: 2.925 ± 0.072	4.262LysSer: 4.262 ± 0.085	4.128LysThr: 4.128 ± 0.09	4.564LysVal: 4.564 ± 0.095	0.578LysTrp: 0.578 ± 0.029	3.666LysTyr: 3.666 ± 0.06	0.001LysXaa: 0.001 ± 0.001
Leu	5.997LeuAla: 5.997 ± 0.082	1.287LeuCys: 1.287 ± 0.039	5.036LeuAsp: 5.036 ± 0.081	5.092LeuGlu: 5.092 ± 0.087	3.387LeuPhe: 3.387 ± 0.077	5.168LeuGly: 5.168 ± 0.089	1.283LeuHis: 1.283 ± 0.043	6.327LeuIle: 6.327 ± 0.118	6.282LeuLys: 6.282 ± 0.104	6.38LeuLeu: 6.38 ± 0.112	2.418LeuMet: 2.418 ± 0.058	4.261LeuAsn: 4.261 ± 0.074	2.72LeuPro: 2.72 ± 0.065	1.858LeuGln: 1.858 ± 0.054	2.937LeuArg: 2.937 ± 0.058	5.977LeuSer: 5.977 ± 0.112	4.409LeuThr: 4.409 ± 0.081	5.165LeuVal: 5.165 ± 0.096	0.525LeuTrp: 0.525 ± 0.025	3.09LeuTyr: 3.09 ± 0.069	0.001LeuXaa: 0.001 ± 0.001
Met	2.429MetAla: 2.429 ± 0.06	0.421MetCys: 0.421 ± 0.023	1.745MetAsp: 1.745 ± 0.056	2.041MetGlu: 2.041 ± 0.065	1.197MetPhe: 1.197 ± 0.042	1.99MetGly: 1.99 ± 0.056	0.475MetHis: 0.475 ± 0.025	2.324MetIle: 2.324 ± 0.064	2.533MetLys: 2.533 ± 0.068	2.704MetLeu: 2.704 ± 0.062	1.0MetMet: 1.0 ± 0.043	1.749MetAsn: 1.749 ± 0.045	1.175MetPro: 1.175 ± 0.039	1.071MetGln: 1.071 ± 0.039	1.204MetArg: 1.204 ± 0.043	2.156MetSer: 2.156 ± 0.053	1.815MetThr: 1.815 ± 0.051	1.89MetVal: 1.89 ± 0.05	0.213MetTrp: 0.213 ± 0.018	1.176MetTyr: 1.176 ± 0.04	0.003MetXaa: 0.003 ± 0.002
Asn	3.761AsnAla: 3.761 ± 0.069	0.731AsnCys: 0.731 ± 0.037	3.138AsnAsp: 3.138 ± 0.077	3.615AsnGlu: 3.615 ± 0.075	1.845AsnPhe: 1.845 ± 0.056	3.948AsnGly: 3.948 ± 0.094	0.84AsnHis: 0.84 ± 0.035	5.098AsnIle: 5.098 ± 0.083	3.785AsnLys: 3.785 ± 0.083	3.926AsnLeu: 3.926 ± 0.077	1.868AsnMet: 1.868 ± 0.047	3.411AsnAsn: 3.411 ± 0.109	2.198AsnPro: 2.198 ± 0.05	1.346AsnGln: 1.346 ± 0.047	1.935AsnArg: 1.935 ± 0.062	3.316AsnSer: 3.316 ± 0.068	2.914AsnThr: 2.914 ± 0.074	4.014AsnVal: 4.014 ± 0.086	0.322AsnTrp: 0.322 ± 0.023	2.195AsnTyr: 2.195 ± 0.06	0.001AsnXaa: 0.001 ± 0.001
Pro	1.985ProAla: 1.985 ± 0.057	0.41ProCys: 0.41 ± 0.022	2.432ProAsp: 2.432 ± 0.069	2.555ProGlu: 2.555 ± 0.06	1.309ProPhe: 1.309 ± 0.05	1.987ProGly: 1.987 ± 0.052	0.461ProHis: 0.461 ± 0.028	1.684ProIle: 1.684 ± 0.053	1.704ProLys: 1.704 ± 0.055	2.348ProLeu: 2.348 ± 0.051	0.741ProMet: 0.741 ± 0.035	1.011ProAsn: 1.011 ± 0.042	0.542ProPro: 0.542 ± 0.028	0.973ProGln: 0.973 ± 0.037	0.777ProArg: 0.777 ± 0.037	1.68ProSer: 1.68 ± 0.046	1.312ProThr: 1.312 ± 0.048	3.003ProVal: 3.003 ± 0.054	0.231ProTrp: 0.231 ± 0.02	1.278ProTyr: 1.278 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	1.936GlnAla: 1.936 ± 0.066	0.321GlnCys: 0.321 ± 0.02	1.341GlnAsp: 1.341 ± 0.042	1.864GlnGlu: 1.864 ± 0.062	1.106GlnPhe: 1.106 ± 0.039	1.465GlnGly: 1.465 ± 0.05	0.41GlnHis: 0.41 ± 0.024	2.509GlnIle: 2.509 ± 0.063	2.218GlnLys: 2.218 ± 0.054	2.488GlnLeu: 2.488 ± 0.063	1.039GlnMet: 1.039 ± 0.042	1.476GlnAsn: 1.476 ± 0.053	0.773GlnPro: 0.773 ± 0.036	0.98GlnGln: 0.98 ± 0.043	1.091GlnArg: 1.091 ± 0.045	1.556GlnSer: 1.556 ± 0.047	1.49GlnThr: 1.49 ± 0.056	1.591GlnVal: 1.591 ± 0.043	0.196GlnTrp: 0.196 ± 0.018	1.231GlnTyr: 1.231 ± 0.047	0.0GlnXaa: 0.0 ± 0.0
Arg	2.135ArgAla: 2.135 ± 0.054	0.51ArgCys: 0.51 ± 0.033	1.955ArgAsp: 1.955 ± 0.052	2.66ArgGlu: 2.66 ± 0.075	1.743ArgPhe: 1.743 ± 0.054	2.096ArgGly: 2.096 ± 0.062	0.644ArgHis: 0.644 ± 0.033	3.345ArgIle: 3.345 ± 0.079	3.129ArgLys: 3.129 ± 0.077	3.211ArgLeu: 3.211 ± 0.074	1.354ArgMet: 1.354 ± 0.049	1.983ArgAsn: 1.983 ± 0.055	1.067ArgPro: 1.067 ± 0.04	1.437ArgGln: 1.437 ± 0.048	1.661ArgArg: 1.661 ± 0.057	1.796ArgSer: 1.796 ± 0.062	1.942ArgThr: 1.942 ± 0.058	2.436ArgVal: 2.436 ± 0.061	0.287ArgTrp: 0.287 ± 0.019	1.707ArgTyr: 1.707 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	4.423SerAla: 4.423 ± 0.078	0.833SerCys: 0.833 ± 0.033	4.583SerAsp: 4.583 ± 0.088	4.203SerGlu: 4.203 ± 0.083	2.88SerPhe: 2.88 ± 0.07	5.342SerGly: 5.342 ± 0.108	0.952SerHis: 0.952 ± 0.036	4.606SerIle: 4.606 ± 0.077	4.077SerLys: 4.077 ± 0.081	5.066SerLeu: 5.066 ± 0.086	1.798SerMet: 1.798 ± 0.052	2.733SerAsn: 2.733 ± 0.071	1.49SerPro: 1.49 ± 0.043	2.062SerGln: 2.062 ± 0.055	2.478SerArg: 2.478 ± 0.064	4.445SerSer: 4.445 ± 0.107	3.09SerThr: 3.09 ± 0.077	4.966SerVal: 4.966 ± 0.097	0.473SerTrp: 0.473 ± 0.028	2.849SerTyr: 2.849 ± 0.065	0.003SerXaa: 0.003 ± 0.002
Thr	4.123ThrAla: 4.123 ± 0.089	0.687ThrCys: 0.687 ± 0.032	3.839ThrAsp: 3.839 ± 0.08	3.237ThrGlu: 3.237 ± 0.083	2.31ThrPhe: 2.31 ± 0.063	4.641ThrGly: 4.641 ± 0.087	0.851ThrHis: 0.851 ± 0.041	4.179ThrIle: 4.179 ± 0.078	3.45ThrLys: 3.45 ± 0.082	4.31ThrLeu: 4.31 ± 0.074	1.343ThrMet: 1.343 ± 0.046	2.268ThrAsn: 2.268 ± 0.058	1.932ThrPro: 1.932 ± 0.063	1.652ThrGln: 1.652 ± 0.055	1.717ThrArg: 1.717 ± 0.046	3.355ThrSer: 3.355 ± 0.074	2.902ThrThr: 2.902 ± 0.093	4.451ThrVal: 4.451 ± 0.093	0.377ThrTrp: 0.377 ± 0.024	2.282ThrTyr: 2.282 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	4.829ValAla: 4.829 ± 0.101	1.263ValCys: 1.263 ± 0.046	4.137ValAsp: 4.137 ± 0.078	4.249ValGlu: 4.249 ± 0.084	3.007ValPhe: 3.007 ± 0.07	4.154ValGly: 4.154 ± 0.087	0.994ValHis: 0.994 ± 0.039	6.375ValIle: 6.375 ± 0.12	5.33ValLys: 5.33 ± 0.108	6.208ValLeu: 6.208 ± 0.101	2.167ValMet: 2.167 ± 0.052	3.864ValAsn: 3.864 ± 0.075	2.258ValPro: 2.258 ± 0.058	1.551ValGln: 1.551 ± 0.053	2.593ValArg: 2.593 ± 0.064	5.179ValSer: 5.179 ± 0.095	4.542ValThr: 4.542 ± 0.097	5.043ValVal: 5.043 ± 0.097	0.473ValTrp: 0.473 ± 0.028	3.069ValTyr: 3.069 ± 0.066	0.001ValXaa: 0.001 ± 0.001
Trp	0.391TrpAla: 0.391 ± 0.024	0.122TrpCys: 0.122 ± 0.015	0.448TrpAsp: 0.448 ± 0.029	0.483TrpGlu: 0.483 ± 0.028	0.346TrpPhe: 0.346 ± 0.023	0.511TrpGly: 0.511 ± 0.026	0.151TrpHis: 0.151 ± 0.015	0.56TrpIle: 0.56 ± 0.026	0.535TrpLys: 0.535 ± 0.03	0.679TrpLeu: 0.679 ± 0.034	0.207TrpMet: 0.207 ± 0.016	0.482TrpAsn: 0.482 ± 0.03	0.127TrpPro: 0.127 ± 0.013	0.249TrpGln: 0.249 ± 0.02	0.258TrpArg: 0.258 ± 0.019	0.372TrpSer: 0.372 ± 0.024	0.291TrpThr: 0.291 ± 0.023	0.427TrpVal: 0.427 ± 0.027	0.06TrpTrp: 0.06 ± 0.011	0.365TrpTyr: 0.365 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.781TyrAla: 2.781 ± 0.064	0.627TyrCys: 0.627 ± 0.031	3.038TyrAsp: 3.038 ± 0.072	3.045TyrGlu: 3.045 ± 0.067	1.904TyrPhe: 1.904 ± 0.056	3.01TyrGly: 3.01 ± 0.069	0.624TyrHis: 0.624 ± 0.03	3.671TyrIle: 3.671 ± 0.073	3.146TyrLys: 3.146 ± 0.071	3.18TyrLeu: 3.18 ± 0.072	1.371TyrMet: 1.371 ± 0.043	2.818TyrAsn: 2.818 ± 0.076	1.295TyrPro: 1.295 ± 0.045	1.019TyrGln: 1.019 ± 0.037	1.767TyrArg: 1.767 ± 0.053	2.851TyrSer: 2.851 ± 0.065	2.534TyrThr: 2.534 ± 0.067	2.88TyrVal: 2.88 ± 0.068	0.319TyrTrp: 0.319 ± 0.021	2.06TyrTyr: 2.06 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.002	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.003XaaGln: 0.003 ± 0.002	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.018XaaXaa: 0.018 ± 0.006

Statistics based on 2189 proteins (714217 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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