Paenibacillus uliginis N3/975

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus uliginis

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5718 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7H1Y2|A0A1X7H1Y2_9BACL Carbohydrate ABC transporter membrane protein 1 CUT1 family OS=Paenibacillus uliginis N3/975 OX=1313296 GN=SAMN05661091_1452 PE=3 SV=1
MM1 pKa = 6.73TTDD4 pKa = 3.78HH5 pKa = 6.88SSSGQALGYY14 pKa = 10.21VYY16 pKa = 10.55QFDD19 pKa = 3.81RR20 pKa = 11.84ATYY23 pKa = 10.11RR24 pKa = 11.84LFQSGVDD31 pKa = 3.51VVEE34 pKa = 4.88IGVEE38 pKa = 4.41DD39 pKa = 3.73IDD41 pKa = 4.65EE42 pKa = 4.21EE43 pKa = 4.43

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7HUD2|A0A1X7HUD2_9BACL Uncharacterized protein OS=Paenibacillus uliginis N3/975 OX=1313296 GN=SAMN05661091_6134 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 9.45QTFKK6 pKa = 11.13PNVSKK11 pKa = 10.88RR12 pKa = 11.84KK13 pKa = 8.93KK14 pKa = 8.63NHH16 pKa = 4.91GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.7VLASRR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.58VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5718

0

5718

1773498

26

4537

310.2

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.301 ± 0.039

0.802 ± 0.01

5.246 ± 0.027

6.956 ± 0.042

4.207 ± 0.026

7.201 ± 0.033

2.102 ± 0.018

7.147 ± 0.034

5.808 ± 0.034

9.977 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.006 ± 0.017

4.092 ± 0.026

3.863 ± 0.02

3.818 ± 0.023

4.743 ± 0.029

6.553 ± 0.026

5.361 ± 0.024

7.01 ± 0.026

1.218 ± 0.012

3.589 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski