Tomato yellow leaf curl betasatellite
Average proteome isoelectric point is 4.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9NJ54|A9NJ54_9VIRU C1 protein OS=Tomato yellow leaf curl betasatellite OX=391090 GN=BC1 PE=4 SV=1
MM1 pKa = 7.23 TIKK4 pKa = 10.74 YY5 pKa = 9.04 NNKK8 pKa = 9.14 RR9 pKa = 11.84 GMEE12 pKa = 3.75 FTIDD16 pKa = 3.48 VKK18 pKa = 11.01 INEE21 pKa = 4.5 DD22 pKa = 3.29 NSILVHH28 pKa = 6.89 IEE30 pKa = 3.83 LFSTQSPALVKK41 pKa = 10.49 KK42 pKa = 10.69 NFMIPYY48 pKa = 8.43 GHH50 pKa = 7.47 DD51 pKa = 3.85 GIIPPFDD58 pKa = 3.98 FNALEE63 pKa = 4.1 EE64 pKa = 5.12 GIQNLLQIMYY74 pKa = 9.39 KK75 pKa = 10.55 DD76 pKa = 3.29 SDD78 pKa = 3.2 IGEE81 pKa = 4.18 FRR83 pKa = 11.84 QEE85 pKa = 4.42 DD86 pKa = 3.94 MVEE89 pKa = 4.28 TIDD92 pKa = 4.35 LLMMEE97 pKa = 5.26 EE98 pKa = 4.57 APLVDD103 pKa = 3.64 IRR105 pKa = 11.84 IGDD108 pKa = 3.88 EE109 pKa = 3.97 YY110 pKa = 11.54 DD111 pKa = 3.26 VCTKK115 pKa = 10.67 VCVV118 pKa = 3.39
Molecular weight: 13.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.355
IPC2_protein 4.304
IPC_protein 4.228
Toseland 4.05
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.088
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.139
Nozaki 4.304
DTASelect 4.482
Thurlkill 4.075
EMBOSS 4.101
Sillero 4.342
Patrickios 3.643
IPC_peptide 4.19
IPC2_peptide 4.329
IPC2.peptide.svr19 4.264
Protein with the highest isoelectric point:
>tr|A9NJ54|A9NJ54_9VIRU C1 protein OS=Tomato yellow leaf curl betasatellite OX=391090 GN=BC1 PE=4 SV=1
MM1 pKa = 7.23 TIKK4 pKa = 10.74 YY5 pKa = 9.04 NNKK8 pKa = 9.14 RR9 pKa = 11.84 GMEE12 pKa = 3.75 FTIDD16 pKa = 3.48 VKK18 pKa = 11.01 INEE21 pKa = 4.5 DD22 pKa = 3.29 NSILVHH28 pKa = 6.89 IEE30 pKa = 3.83 LFSTQSPALVKK41 pKa = 10.49 KK42 pKa = 10.69 NFMIPYY48 pKa = 8.43 GHH50 pKa = 7.47 DD51 pKa = 3.85 GIIPPFDD58 pKa = 3.98 FNALEE63 pKa = 4.1 EE64 pKa = 5.12 GIQNLLQIMYY74 pKa = 9.39 KK75 pKa = 10.55 DD76 pKa = 3.29 SDD78 pKa = 3.2 IGEE81 pKa = 4.18 FRR83 pKa = 11.84 QEE85 pKa = 4.42 DD86 pKa = 3.94 MVEE89 pKa = 4.28 TIDD92 pKa = 4.35 LLMMEE97 pKa = 5.26 EE98 pKa = 4.57 APLVDD103 pKa = 3.64 IRR105 pKa = 11.84 IGDD108 pKa = 3.88 EE109 pKa = 3.97 YY110 pKa = 11.54 DD111 pKa = 3.26 VCTKK115 pKa = 10.67 VCVV118 pKa = 3.39
Molecular weight: 13.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.355
IPC2_protein 4.304
IPC_protein 4.228
Toseland 4.05
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.088
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.139
Nozaki 4.304
DTASelect 4.482
Thurlkill 4.075
EMBOSS 4.101
Sillero 4.342
Patrickios 3.643
IPC_peptide 4.19
IPC2_peptide 4.329
IPC2.peptide.svr19 4.264
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.542 ± 0.0
1.695 ± 0.0
9.322 ± 0.0
9.322 ± 0.0
5.085 ± 0.0
5.085 ± 0.0
1.695 ± 0.0
11.864 ± 0.0
5.932 ± 0.0
7.627 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.932 ± 0.0
5.932 ± 0.0
4.237 ± 0.0
3.39 ± 0.0
2.542 ± 0.0
3.39 ± 0.0
4.237 ± 0.0
6.78 ± 0.0
0.0 ± 0.0
3.39 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here