Citrobacter phage SH4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Citrobacter virus SH4

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172JGD9|A0A172JGD9_9CAUD Tail fibers protein OS=Citrobacter phage SH4 OX=1805467 GN=sh4_0040 PE=4 SV=1
MM1 pKa = 6.77VQGSIDD7 pKa = 3.7IGPLRR12 pKa = 11.84ANLGDD17 pKa = 3.74QEE19 pKa = 4.19IDD21 pKa = 3.48SVKK24 pKa = 10.44YY25 pKa = 10.65QITQRR30 pKa = 11.84LDD32 pKa = 3.04QMGLVGFKK40 pKa = 11.08LEE42 pKa = 4.23GDD44 pKa = 3.85VLSYY48 pKa = 11.34YY49 pKa = 10.15PFEE52 pKa = 4.65EE53 pKa = 4.29YY54 pKa = 10.52QDD56 pKa = 3.49VKK58 pKa = 10.94EE59 pKa = 4.45VVRR62 pKa = 11.84EE63 pKa = 4.03VPVQVSPFAGEE74 pKa = 4.23VVSPRR79 pKa = 11.84SYY81 pKa = 10.98SVEE84 pKa = 3.75QHH86 pKa = 6.59PEE88 pKa = 4.05GPAVPVSSMEE98 pKa = 3.54QSYY101 pKa = 11.01PEE103 pKa = 3.94VRR105 pKa = 11.84VSSGEE110 pKa = 4.21SPVVEE115 pKa = 4.16PMDD118 pKa = 4.21TIEE121 pKa = 4.53PLVPDD126 pKa = 4.06MTPPKK131 pKa = 10.56AA132 pKa = 3.46

Molecular weight: 14.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172JGF7|A0A172JGF7_9CAUD Tail tubular protein B OS=Citrobacter phage SH4 OX=1805467 GN=sh4_0035 PE=4 SV=1
MM1 pKa = 7.5SLALLIAIGYY11 pKa = 8.61GLIAYY16 pKa = 8.18VLVRR20 pKa = 11.84DD21 pKa = 3.6INKK24 pKa = 9.28ARR26 pKa = 11.84KK27 pKa = 8.51VYY29 pKa = 9.84KK30 pKa = 10.31FNYY33 pKa = 8.37VRR35 pKa = 11.84LGRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FMRR50 pKa = 11.84NLANVWDD57 pKa = 4.02IATLGSKK64 pKa = 10.07LL65 pKa = 3.58

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12332

41

1298

251.7

27.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.66 ± 0.433

0.933 ± 0.154

6.333 ± 0.22

6.682 ± 0.321

3.649 ± 0.184

8.052 ± 0.342

1.606 ± 0.167

4.817 ± 0.152

6.536 ± 0.393

8.352 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.124

4.16 ± 0.245

3.884 ± 0.227

4.127 ± 0.397

5.417 ± 0.19

6.147 ± 0.255

5.765 ± 0.298

7.071 ± 0.312

1.565 ± 0.182

3.365 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski