Citrus vein enation virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Enamovirus

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S0EQ55|S0EQ55_9LUTE Serine protease OS=Citrus vein enation virus OX=1301220 PE=4 SV=1
MM1 pKa = 7.64PCYY4 pKa = 10.06HH5 pKa = 7.28VIPYY9 pKa = 10.42DD10 pKa = 3.63YY11 pKa = 9.78FQPIHH16 pKa = 6.42SSNWLGASLLALINYY31 pKa = 9.33VGMGCPTQEE40 pKa = 3.97GTPCEE45 pKa = 4.32EE46 pKa = 4.44PTCVALPYY54 pKa = 10.84VLLCALALLWGHH66 pKa = 6.48PRR68 pKa = 11.84SCGRR72 pKa = 11.84RR73 pKa = 11.84GGVSLEE79 pKa = 3.86ALTLPRR85 pKa = 11.84GFNIVGPPDD94 pKa = 3.6FLWYY98 pKa = 10.28FRR100 pKa = 11.84ICAARR105 pKa = 11.84IGLIIPQSYY114 pKa = 8.36WRR116 pKa = 11.84DD117 pKa = 3.49DD118 pKa = 3.31GRR120 pKa = 11.84RR121 pKa = 11.84AIPLTHH127 pKa = 6.72CALFTSLPCWLMAASGKK144 pKa = 10.4AEE146 pKa = 4.05YY147 pKa = 10.02HH148 pKa = 5.56CQGYY152 pKa = 8.25VAPEE156 pKa = 3.83LQLPGDD162 pKa = 4.34GPEE165 pKa = 3.82HH166 pKa = 5.32TVWVEE171 pKa = 3.85EE172 pKa = 5.09KK173 pKa = 8.74WACWHH178 pKa = 5.74EE179 pKa = 4.59LIVGLLGTHH188 pKa = 6.11TNVASILRR196 pKa = 11.84VLPDD200 pKa = 3.5GGVTPHH206 pKa = 6.04SQVLGKK212 pKa = 10.62RR213 pKa = 11.84NLIVDD218 pKa = 4.55AFTSISTMAVGTDD231 pKa = 3.31QPPPNRR237 pKa = 11.84YY238 pKa = 8.58MDD240 pKa = 4.31DD241 pKa = 3.74CSLTRR246 pKa = 11.84LFCLRR251 pKa = 11.84EE252 pKa = 4.17PYY254 pKa = 10.7LSGYY258 pKa = 8.88DD259 pKa = 3.37TDD261 pKa = 4.28NFSPGLHH268 pKa = 5.96ITLPWPEE275 pKa = 3.84IPEE278 pKa = 4.3GGEE281 pKa = 3.85YY282 pKa = 10.11LAHH285 pKa = 6.77SFAEE289 pKa = 4.14LDD291 pKa = 3.69QEE293 pKa = 4.62FADD296 pKa = 4.69LLAHH300 pKa = 6.38LHH302 pKa = 5.15VHH304 pKa = 7.21DD305 pKa = 5.74DD306 pKa = 4.04EE307 pKa = 6.17EE308 pKa = 5.35DD309 pKa = 3.72LGWFSDD315 pKa = 3.98DD316 pKa = 4.29GEE318 pKa = 5.01GDD320 pKa = 3.42IEE322 pKa = 4.92NPPAPHH328 pKa = 6.1NTDD331 pKa = 3.38EE332 pKa = 4.52EE333 pKa = 4.77DD334 pKa = 4.93FGDD337 pKa = 4.94SDD339 pKa = 5.54DD340 pKa = 5.18DD341 pKa = 5.28DD342 pKa = 6.75SIGGNAGSGPEE353 pKa = 3.76AA354 pKa = 4.5

Molecular weight:
38.96 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S0ERJ8|S0ERJ8_9LUTE CP OS=Citrus vein enation virus OX=1301220 PE=4 SV=1
MM1 pKa = 7.36VSRR4 pKa = 11.84NQRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84NRR11 pKa = 11.84PRR13 pKa = 11.84RR14 pKa = 11.84MRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84QAPNRR23 pKa = 11.84VVVMGNNPTRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84NAPARR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84AVNRR49 pKa = 11.84STGEE53 pKa = 3.5MRR55 pKa = 11.84PYY57 pKa = 10.38HH58 pKa = 7.16LYY60 pKa = 10.83GLKK63 pKa = 10.92CNDD66 pKa = 3.19KK67 pKa = 11.03GYY69 pKa = 8.96LTFGPSGQTPSLGGGILKK87 pKa = 10.48AFSEE91 pKa = 4.48YY92 pKa = 10.58KK93 pKa = 8.8ITQLRR98 pKa = 11.84VQWKK102 pKa = 8.54AQASSTAAGSMAIQIGLGTSLTALDD127 pKa = 3.68NRR129 pKa = 11.84AISFKK134 pKa = 9.98LTSSGQRR141 pKa = 11.84VFTARR146 pKa = 11.84DD147 pKa = 3.21LGGDD151 pKa = 2.87GRR153 pKa = 11.84MYY155 pKa = 10.93NSSNEE160 pKa = 3.99DD161 pKa = 3.32QFRR164 pKa = 11.84LAYY167 pKa = 9.74QGNGDD172 pKa = 3.82STLGGDD178 pKa = 4.17LLCFFRR184 pKa = 11.84LEE186 pKa = 4.05TRR188 pKa = 11.84LPKK191 pKa = 10.53

Molecular weight:
21.45 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3264

191

1323

652.8

72.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.261 ± 0.106

2.145 ± 0.307

4.504 ± 0.537

5.637 ± 0.384

3.37 ± 0.224

7.629 ± 0.443

1.777 ± 0.39

4.289 ± 0.199

4.626 ± 0.594

10.968 ± 1.007

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.347

3.401 ± 0.385

5.974 ± 0.584

3.676 ± 0.238

7.414 ± 1.002

8.027 ± 0.52

5.392 ± 0.208

6.342 ± 0.509

2.022 ± 0.237

2.911 ± 0.247

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski