Citrus vein enation virus
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S0EQ55|S0EQ55_9LUTE Serine protease OS=Citrus vein enation virus OX=1301220 PE=4 SV=1
MM1 pKa = 7.64 PCYY4 pKa = 10.06 HH5 pKa = 7.28 VIPYY9 pKa = 10.42 DD10 pKa = 3.63 YY11 pKa = 9.78 FQPIHH16 pKa = 6.42 SSNWLGASLLALINYY31 pKa = 9.33 VGMGCPTQEE40 pKa = 3.97 GTPCEE45 pKa = 4.32 EE46 pKa = 4.44 PTCVALPYY54 pKa = 10.84 VLLCALALLWGHH66 pKa = 6.48 PRR68 pKa = 11.84 SCGRR72 pKa = 11.84 RR73 pKa = 11.84 GGVSLEE79 pKa = 3.86 ALTLPRR85 pKa = 11.84 GFNIVGPPDD94 pKa = 3.6 FLWYY98 pKa = 10.28 FRR100 pKa = 11.84 ICAARR105 pKa = 11.84 IGLIIPQSYY114 pKa = 8.36 WRR116 pKa = 11.84 DD117 pKa = 3.49 DD118 pKa = 3.31 GRR120 pKa = 11.84 RR121 pKa = 11.84 AIPLTHH127 pKa = 6.72 CALFTSLPCWLMAASGKK144 pKa = 10.4 AEE146 pKa = 4.05 YY147 pKa = 10.02 HH148 pKa = 5.56 CQGYY152 pKa = 8.25 VAPEE156 pKa = 3.83 LQLPGDD162 pKa = 4.34 GPEE165 pKa = 3.82 HH166 pKa = 5.32 TVWVEE171 pKa = 3.85 EE172 pKa = 5.09 KK173 pKa = 8.74 WACWHH178 pKa = 5.74 EE179 pKa = 4.59 LIVGLLGTHH188 pKa = 6.11 TNVASILRR196 pKa = 11.84 VLPDD200 pKa = 3.5 GGVTPHH206 pKa = 6.04 SQVLGKK212 pKa = 10.62 RR213 pKa = 11.84 NLIVDD218 pKa = 4.55 AFTSISTMAVGTDD231 pKa = 3.31 QPPPNRR237 pKa = 11.84 YY238 pKa = 8.58 MDD240 pKa = 4.31 DD241 pKa = 3.74 CSLTRR246 pKa = 11.84 LFCLRR251 pKa = 11.84 EE252 pKa = 4.17 PYY254 pKa = 10.7 LSGYY258 pKa = 8.88 DD259 pKa = 3.37 TDD261 pKa = 4.28 NFSPGLHH268 pKa = 5.96 ITLPWPEE275 pKa = 3.84 IPEE278 pKa = 4.3 GGEE281 pKa = 3.85 YY282 pKa = 10.11 LAHH285 pKa = 6.77 SFAEE289 pKa = 4.14 LDD291 pKa = 3.69 QEE293 pKa = 4.62 FADD296 pKa = 4.69 LLAHH300 pKa = 6.38 LHH302 pKa = 5.15 VHH304 pKa = 7.21 DD305 pKa = 5.74 DD306 pKa = 4.04 EE307 pKa = 6.17 EE308 pKa = 5.35 DD309 pKa = 3.72 LGWFSDD315 pKa = 3.98 DD316 pKa = 4.29 GEE318 pKa = 5.01 GDD320 pKa = 3.42 IEE322 pKa = 4.92 NPPAPHH328 pKa = 6.1 NTDD331 pKa = 3.38 EE332 pKa = 4.52 EE333 pKa = 4.77 DD334 pKa = 4.93 FGDD337 pKa = 4.94 SDD339 pKa = 5.54 DD340 pKa = 5.18 DD341 pKa = 5.28 DD342 pKa = 6.75 SIGGNAGSGPEE353 pKa = 3.76 AA354 pKa = 4.5
Molecular weight: 38.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.255
IPC2_protein 4.393
IPC_protein 4.368
Toseland 4.202
ProMoST 4.495
Dawson 4.342
Bjellqvist 4.482
Wikipedia 4.228
Rodwell 4.215
Grimsley 4.113
Solomon 4.329
Lehninger 4.291
Nozaki 4.444
DTASelect 4.647
Thurlkill 4.215
EMBOSS 4.253
Sillero 4.495
Patrickios 0.617
IPC_peptide 4.342
IPC2_peptide 4.482
IPC2.peptide.svr19 4.378
Protein with the highest isoelectric point:
>tr|S0ERJ8|S0ERJ8_9LUTE CP OS=Citrus vein enation virus OX=1301220 PE=4 SV=1
MM1 pKa = 7.36 VSRR4 pKa = 11.84 NQRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 NRR11 pKa = 11.84 PRR13 pKa = 11.84 RR14 pKa = 11.84 MRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 QAPNRR23 pKa = 11.84 VVVMGNNPTRR33 pKa = 11.84 SRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 NAPARR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 AVNRR49 pKa = 11.84 STGEE53 pKa = 3.5 MRR55 pKa = 11.84 PYY57 pKa = 10.38 HH58 pKa = 7.16 LYY60 pKa = 10.83 GLKK63 pKa = 10.92 CNDD66 pKa = 3.19 KK67 pKa = 11.03 GYY69 pKa = 8.96 LTFGPSGQTPSLGGGILKK87 pKa = 10.48 AFSEE91 pKa = 4.48 YY92 pKa = 10.58 KK93 pKa = 8.8 ITQLRR98 pKa = 11.84 VQWKK102 pKa = 8.54 AQASSTAAGSMAIQIGLGTSLTALDD127 pKa = 3.68 NRR129 pKa = 11.84 AISFKK134 pKa = 9.98 LTSSGQRR141 pKa = 11.84 VFTARR146 pKa = 11.84 DD147 pKa = 3.21 LGGDD151 pKa = 2.87 GRR153 pKa = 11.84 MYY155 pKa = 10.93 NSSNEE160 pKa = 3.99 DD161 pKa = 3.32 QFRR164 pKa = 11.84 LAYY167 pKa = 9.74 QGNGDD172 pKa = 3.82 STLGGDD178 pKa = 4.17 LLCFFRR184 pKa = 11.84 LEE186 pKa = 4.05 TRR188 pKa = 11.84 LPKK191 pKa = 10.53
Molecular weight: 21.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.158
IPC2_protein 10.262
IPC_protein 11.564
Toseland 11.681
ProMoST 12.164
Dawson 11.696
Bjellqvist 11.667
Wikipedia 12.149
Rodwell 11.418
Grimsley 11.74
Solomon 12.164
Lehninger 12.076
Nozaki 11.681
DTASelect 11.667
Thurlkill 11.681
EMBOSS 12.179
Sillero 11.681
Patrickios 11.125
IPC_peptide 12.164
IPC2_peptide 11.155
IPC2.peptide.svr19 9.329
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3264
191
1323
652.8
72.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.261 ± 0.106
2.145 ± 0.307
4.504 ± 0.537
5.637 ± 0.384
3.37 ± 0.224
7.629 ± 0.443
1.777 ± 0.39
4.289 ± 0.199
4.626 ± 0.594
10.968 ± 1.007
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.604 ± 0.347
3.401 ± 0.385
5.974 ± 0.584
3.676 ± 0.238
7.414 ± 1.002
8.027 ± 0.52
5.392 ± 0.208
6.342 ± 0.509
2.022 ± 0.237
2.911 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here