Amino acid dipepetide frequency for Roseovarius sp. TE539

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.762AlaAla: 16.762 ± 0.195	1.175AlaCys: 1.175 ± 0.039	7.092AlaAsp: 7.092 ± 0.084	8.22AlaGlu: 8.22 ± 0.12	4.084AlaPhe: 4.084 ± 0.067	11.538AlaGly: 11.538 ± 0.125	2.587AlaHis: 2.587 ± 0.052	5.51AlaIle: 5.51 ± 0.076	2.874AlaLys: 2.874 ± 0.056	13.259AlaLeu: 13.259 ± 0.131	3.785AlaMet: 3.785 ± 0.067	2.52AlaAsn: 2.52 ± 0.052	5.984AlaPro: 5.984 ± 0.09	3.943AlaGln: 3.943 ± 0.065	10.325AlaArg: 10.325 ± 0.138	4.979AlaSer: 4.979 ± 0.065	5.724AlaThr: 5.724 ± 0.08	8.362AlaVal: 8.362 ± 0.106	1.477AlaTrp: 1.477 ± 0.041	2.319AlaTyr: 2.319 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	1.149CysAla: 1.149 ± 0.039	0.118CysCys: 0.118 ± 0.012	0.702CysAsp: 0.702 ± 0.027	0.525CysGlu: 0.525 ± 0.022	0.357CysPhe: 0.357 ± 0.016	1.061CysGly: 1.061 ± 0.032	0.282CysHis: 0.282 ± 0.017	0.415CysIle: 0.415 ± 0.019	0.19CysLys: 0.19 ± 0.014	0.918CysLeu: 0.918 ± 0.029	0.2CysMet: 0.2 ± 0.014	0.222CysAsn: 0.222 ± 0.013	0.565CysPro: 0.565 ± 0.025	0.21CysGln: 0.21 ± 0.013	0.728CysArg: 0.728 ± 0.027	0.421CysSer: 0.421 ± 0.023	0.452CysThr: 0.452 ± 0.024	0.645CysVal: 0.645 ± 0.026	0.128CysTrp: 0.128 ± 0.012	0.213CysTyr: 0.213 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	7.375AspAla: 7.375 ± 0.089	0.584AspCys: 0.584 ± 0.023	3.584AspAsp: 3.584 ± 0.08	3.718AspGlu: 3.718 ± 0.059	2.294AspPhe: 2.294 ± 0.045	5.969AspGly: 5.969 ± 0.086	1.53AspHis: 1.53 ± 0.043	3.171AspIle: 3.171 ± 0.054	1.498AspLys: 1.498 ± 0.038	6.505AspLeu: 6.505 ± 0.088	1.769AspMet: 1.769 ± 0.041	1.278AspAsn: 1.278 ± 0.038	4.104AspPro: 4.104 ± 0.068	1.629AspGln: 1.629 ± 0.035	5.043AspArg: 5.043 ± 0.078	2.378AspSer: 2.378 ± 0.046	3.241AspThr: 3.241 ± 0.063	4.227AspVal: 4.227 ± 0.061	1.232AspTrp: 1.232 ± 0.034	1.468AspTyr: 1.468 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	8.38GluAla: 8.38 ± 0.114	0.399GluCys: 0.399 ± 0.021	3.733GluAsp: 3.733 ± 0.056	3.897GluGlu: 3.897 ± 0.079	1.952GluPhe: 1.952 ± 0.04	5.066GluGly: 5.066 ± 0.082	1.227GluHis: 1.227 ± 0.037	3.744GluIle: 3.744 ± 0.067	2.188GluLys: 2.188 ± 0.051	5.084GluLeu: 5.084 ± 0.082	1.999GluMet: 1.999 ± 0.046	1.782GluAsn: 1.782 ± 0.039	2.617GluPro: 2.617 ± 0.051	1.8GluGln: 1.8 ± 0.041	4.778GluArg: 4.778 ± 0.072	2.501GluSer: 2.501 ± 0.053	4.272GluThr: 4.272 ± 0.068	4.352GluVal: 4.352 ± 0.07	0.786GluTrp: 0.786 ± 0.023	1.1GluTyr: 1.1 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.179PheAla: 4.179 ± 0.077	0.425PheCys: 0.425 ± 0.02	2.794PheAsp: 2.794 ± 0.049	2.284PheGlu: 2.284 ± 0.045	1.389PhePhe: 1.389 ± 0.042	3.557PheGly: 3.557 ± 0.066	0.834PheHis: 0.834 ± 0.03	1.514PheIle: 1.514 ± 0.035	0.743PheLys: 0.743 ± 0.026	3.382PheLeu: 3.382 ± 0.061	0.872PheMet: 0.872 ± 0.025	0.994PheAsn: 0.994 ± 0.034	1.482PhePro: 1.482 ± 0.037	0.933PheGln: 0.933 ± 0.032	2.425PheArg: 2.425 ± 0.051	2.072PheSer: 2.072 ± 0.043	2.045PheThr: 2.045 ± 0.044	2.578PheVal: 2.578 ± 0.047	0.581PheTrp: 0.581 ± 0.022	0.857PheTyr: 0.857 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.336GlyAla: 10.336 ± 0.114	0.959GlyCys: 0.959 ± 0.03	5.102GlyAsp: 5.102 ± 0.084	4.992GlyGlu: 4.992 ± 0.073	3.699GlyPhe: 3.699 ± 0.063	7.874GlyGly: 7.874 ± 0.131	2.246GlyHis: 2.246 ± 0.055	4.458GlyIle: 4.458 ± 0.063	2.937GlyLys: 2.937 ± 0.058	9.53GlyLeu: 9.53 ± 0.106	2.778GlyMet: 2.778 ± 0.061	2.214GlyAsn: 2.214 ± 0.057	4.146GlyPro: 4.146 ± 0.059	2.901GlyGln: 2.901 ± 0.049	6.858GlyArg: 6.858 ± 0.08	4.189GlySer: 4.189 ± 0.065	5.029GlyThr: 5.029 ± 0.067	6.342GlyVal: 6.342 ± 0.082	1.523GlyTrp: 1.523 ± 0.037	2.328GlyTyr: 2.328 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.636HisAla: 2.636 ± 0.051	0.272HisCys: 0.272 ± 0.015	1.598HisAsp: 1.598 ± 0.038	1.318HisGlu: 1.318 ± 0.033	0.872HisPhe: 0.872 ± 0.027	2.181HisGly: 2.181 ± 0.05	0.633HisHis: 0.633 ± 0.028	0.856HisIle: 0.856 ± 0.028	0.485HisLys: 0.485 ± 0.023	2.184HisLeu: 2.184 ± 0.044	0.555HisMet: 0.555 ± 0.025	0.477HisAsn: 0.477 ± 0.02	1.506HisPro: 1.506 ± 0.035	0.549HisGln: 0.549 ± 0.023	1.532HisArg: 1.532 ± 0.038	0.955HisSer: 0.955 ± 0.031	0.827HisThr: 0.827 ± 0.027	1.775HisVal: 1.775 ± 0.043	0.373HisTrp: 0.373 ± 0.017	0.61HisTyr: 0.61 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.453IleAla: 6.453 ± 0.08	0.641IleCys: 0.641 ± 0.026	3.264IleAsp: 3.264 ± 0.058	3.501IleGlu: 3.501 ± 0.062	1.685IlePhe: 1.685 ± 0.038	4.681IleGly: 4.681 ± 0.069	0.949IleHis: 0.949 ± 0.031	2.08IleIle: 2.08 ± 0.051	1.184IleLys: 1.184 ± 0.039	4.677IleLeu: 4.677 ± 0.066	1.088IleMet: 1.088 ± 0.037	1.272IleAsn: 1.272 ± 0.036	2.255IlePro: 2.255 ± 0.047	0.99IleGln: 0.99 ± 0.033	3.416IleArg: 3.416 ± 0.06	2.957IleSer: 2.957 ± 0.054	2.869IleThr: 2.869 ± 0.051	3.605IleVal: 3.605 ± 0.062	0.667IleTrp: 0.667 ± 0.026	1.104IleTyr: 1.104 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.346LysAla: 3.346 ± 0.066	0.195LysCys: 0.195 ± 0.016	1.502LysAsp: 1.502 ± 0.043	1.421LysGlu: 1.421 ± 0.044	0.797LysPhe: 0.797 ± 0.027	2.411LysGly: 2.411 ± 0.046	0.523LysHis: 0.523 ± 0.023	1.383LysIle: 1.383 ± 0.035	1.056LysLys: 1.056 ± 0.032	2.658LysLeu: 2.658 ± 0.052	0.72LysMet: 0.72 ± 0.03	0.647LysAsn: 0.647 ± 0.027	1.689LysPro: 1.689 ± 0.039	0.807LysGln: 0.807 ± 0.025	2.112LysArg: 2.112 ± 0.049	1.659LysSer: 1.659 ± 0.033	1.71LysThr: 1.71 ± 0.044	1.959LysVal: 1.959 ± 0.045	0.356LysTrp: 0.356 ± 0.017	0.554LysTyr: 0.554 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	12.41LeuAla: 12.41 ± 0.139	1.006LeuCys: 1.006 ± 0.03	6.384LeuAsp: 6.384 ± 0.081	5.997LeuGlu: 5.997 ± 0.086	3.281LeuPhe: 3.281 ± 0.061	8.367LeuGly: 8.367 ± 0.09	2.087LeuHis: 2.087 ± 0.05	4.71LeuIle: 4.71 ± 0.071	2.833LeuLys: 2.833 ± 0.048	8.742LeuLeu: 8.742 ± 0.134	2.573LeuMet: 2.573 ± 0.055	2.537LeuAsn: 2.537 ± 0.059	5.362LeuPro: 5.362 ± 0.071	2.623LeuGln: 2.623 ± 0.048	7.814LeuArg: 7.814 ± 0.095	6.394LeuSer: 6.394 ± 0.083	5.541LeuThr: 5.541 ± 0.069	6.875LeuVal: 6.875 ± 0.101	1.267LeuTrp: 1.267 ± 0.03	1.898LeuTyr: 1.898 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	3.548MetAla: 3.548 ± 0.058	0.212MetCys: 0.212 ± 0.014	1.498MetAsp: 1.498 ± 0.039	1.41MetGlu: 1.41 ± 0.037	0.775MetPhe: 0.775 ± 0.031	2.28MetGly: 2.28 ± 0.051	0.512MetHis: 0.512 ± 0.022	1.588MetIle: 1.588 ± 0.037	1.01MetLys: 1.01 ± 0.03	2.572MetLeu: 2.572 ± 0.06	0.777MetMet: 0.777 ± 0.027	0.822MetAsn: 0.822 ± 0.03	1.595MetPro: 1.595 ± 0.042	0.91MetGln: 0.91 ± 0.032	2.144MetArg: 2.144 ± 0.052	1.671MetSer: 1.671 ± 0.039	2.161MetThr: 2.161 ± 0.043	1.816MetVal: 1.816 ± 0.044	0.249MetTrp: 0.249 ± 0.017	0.316MetTyr: 0.316 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.061AsnAla: 3.061 ± 0.049	0.231AsnCys: 0.231 ± 0.014	1.428AsnAsp: 1.428 ± 0.041	1.217AsnGlu: 1.217 ± 0.036	0.857AsnPhe: 0.857 ± 0.032	2.233AsnGly: 2.233 ± 0.053	0.504AsnHis: 0.504 ± 0.02	1.254AsnIle: 1.254 ± 0.036	0.573AsnLys: 0.573 ± 0.028	2.429AsnLeu: 2.429 ± 0.051	0.629AsnMet: 0.629 ± 0.027	0.6AsnAsn: 0.6 ± 0.026	1.748AsnPro: 1.748 ± 0.042	0.617AsnGln: 0.617 ± 0.026	1.823AsnArg: 1.823 ± 0.04	1.041AsnSer: 1.041 ± 0.036	1.298AsnThr: 1.298 ± 0.038	1.743AsnVal: 1.743 ± 0.042	0.396AsnTrp: 0.396 ± 0.02	0.579AsnTyr: 0.579 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.976ProAla: 5.976 ± 0.101	0.418ProCys: 0.418 ± 0.019	4.364ProAsp: 4.364 ± 0.072	4.492ProGlu: 4.492 ± 0.071	1.97ProPhe: 1.97 ± 0.045	5.355ProGly: 5.355 ± 0.082	1.132ProHis: 1.132 ± 0.035	1.938ProIle: 1.938 ± 0.045	1.29ProLys: 1.29 ± 0.038	4.562ProLeu: 4.562 ± 0.065	1.255ProMet: 1.255 ± 0.032	1.12ProAsn: 1.12 ± 0.032	2.553ProPro: 2.553 ± 0.058	1.471ProGln: 1.471 ± 0.036	3.392ProArg: 3.392 ± 0.064	2.333ProSer: 2.333 ± 0.049	2.066ProThr: 2.066 ± 0.042	4.645ProVal: 4.645 ± 0.064	0.66ProTrp: 0.66 ± 0.024	1.142ProTyr: 1.142 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.778GlnAla: 3.778 ± 0.061	0.223GlnCys: 0.223 ± 0.015	1.636GlnAsp: 1.636 ± 0.036	1.607GlnGlu: 1.607 ± 0.042	0.968GlnPhe: 0.968 ± 0.035	2.434GlnGly: 2.434 ± 0.053	0.556GlnHis: 0.556 ± 0.022	1.658GlnIle: 1.658 ± 0.043	0.934GlnLys: 0.934 ± 0.034	2.472GlnLeu: 2.472 ± 0.053	0.922GlnMet: 0.922 ± 0.029	0.746GlnAsn: 0.746 ± 0.026	1.41GlnPro: 1.41 ± 0.035	0.94GlnGln: 0.94 ± 0.03	2.065GlnArg: 2.065 ± 0.042	1.564GlnSer: 1.564 ± 0.037	1.554GlnThr: 1.554 ± 0.039	2.157GlnVal: 2.157 ± 0.047	0.398GlnTrp: 0.398 ± 0.02	0.52GlnTyr: 0.52 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.116ArgAla: 9.116 ± 0.11	0.585ArgCys: 0.585 ± 0.023	5.011ArgAsp: 5.011 ± 0.075	4.652ArgGlu: 4.652 ± 0.069	2.869ArgPhe: 2.869 ± 0.049	5.52ArgGly: 5.52 ± 0.07	1.97ArgHis: 1.97 ± 0.052	4.373ArgIle: 4.373 ± 0.058	2.395ArgLys: 2.395 ± 0.051	8.173ArgLeu: 8.173 ± 0.097	2.269ArgMet: 2.269 ± 0.048	1.887ArgAsn: 1.887 ± 0.044	3.854ArgPro: 3.854 ± 0.066	2.404ArgGln: 2.404 ± 0.051	6.415ArgArg: 6.415 ± 0.098	3.512ArgSer: 3.512 ± 0.062	3.413ArgThr: 3.413 ± 0.057	5.141ArgVal: 5.141 ± 0.077	0.986ArgTrp: 0.986 ± 0.031	1.643ArgTyr: 1.643 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	5.615SerAla: 5.615 ± 0.067	0.459SerCys: 0.459 ± 0.021	3.334SerAsp: 3.334 ± 0.047	2.974SerGlu: 2.974 ± 0.057	2.039SerPhe: 2.039 ± 0.042	5.697SerGly: 5.697 ± 0.078	1.083SerHis: 1.083 ± 0.033	2.236SerIle: 2.236 ± 0.047	1.194SerLys: 1.194 ± 0.036	4.627SerLeu: 4.627 ± 0.062	1.345SerMet: 1.345 ± 0.041	1.176SerAsn: 1.176 ± 0.035	2.492SerPro: 2.492 ± 0.046	1.316SerGln: 1.316 ± 0.035	3.671SerArg: 3.671 ± 0.064	2.272SerSer: 2.272 ± 0.059	2.343SerThr: 2.343 ± 0.042	3.7SerVal: 3.7 ± 0.059	0.683SerTrp: 0.683 ± 0.025	1.164SerTyr: 1.164 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	6.238ThrAla: 6.238 ± 0.098	0.516ThrCys: 0.516 ± 0.022	3.226ThrAsp: 3.226 ± 0.063	3.018ThrGlu: 3.018 ± 0.058	1.801ThrPhe: 1.801 ± 0.047	5.98ThrGly: 5.98 ± 0.078	1.163ThrHis: 1.163 ± 0.031	2.551ThrIle: 2.551 ± 0.049	1.146ThrLys: 1.146 ± 0.03	5.851ThrLeu: 5.851 ± 0.076	1.285ThrMet: 1.285 ± 0.036	1.135ThrAsn: 1.135 ± 0.033	3.269ThrPro: 3.269 ± 0.059	1.4ThrGln: 1.4 ± 0.037	3.972ThrArg: 3.972 ± 0.054	2.495ThrSer: 2.495 ± 0.047	2.736ThrThr: 2.736 ± 0.062	4.352ThrVal: 4.352 ± 0.06	0.694ThrTrp: 0.694 ± 0.024	1.18ThrTyr: 1.18 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	8.492ValAla: 8.492 ± 0.097	0.671ValCys: 0.671 ± 0.027	4.103ValAsp: 4.103 ± 0.057	4.455ValGlu: 4.455 ± 0.071	2.807ValPhe: 2.807 ± 0.057	5.239ValGly: 5.239 ± 0.079	1.429ValHis: 1.429 ± 0.039	4.243ValIle: 4.243 ± 0.059	1.975ValLys: 1.975 ± 0.041	7.323ValLeu: 7.323 ± 0.092	2.144ValMet: 2.144 ± 0.047	1.865ValAsn: 1.865 ± 0.039	3.704ValPro: 3.704 ± 0.067	1.924ValGln: 1.924 ± 0.042	4.77ValArg: 4.77 ± 0.07	4.167ValSer: 4.167 ± 0.064	4.851ValThr: 4.851 ± 0.063	5.355ValVal: 5.355 ± 0.081	0.935ValTrp: 0.935 ± 0.031	1.451ValTyr: 1.451 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.329TrpAla: 1.329 ± 0.037	0.159TrpCys: 0.159 ± 0.013	0.731TrpAsp: 0.731 ± 0.03	0.692TrpGlu: 0.692 ± 0.027	0.559TrpPhe: 0.559 ± 0.024	0.99TrpGly: 0.99 ± 0.032	0.388TrpHis: 0.388 ± 0.019	0.747TrpIle: 0.747 ± 0.028	0.479TrpLys: 0.479 ± 0.023	1.624TrpLeu: 1.624 ± 0.042	0.391TrpMet: 0.391 ± 0.018	0.423TrpAsn: 0.423 ± 0.021	0.756TrpPro: 0.756 ± 0.024	0.579TrpGln: 0.579 ± 0.022	1.195TrpArg: 1.195 ± 0.035	0.788TrpSer: 0.788 ± 0.031	0.805TrpThr: 0.805 ± 0.027	0.816TrpVal: 0.816 ± 0.026	0.231TrpTrp: 0.231 ± 0.014	0.298TrpTyr: 0.298 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.339TyrAla: 2.339 ± 0.046	0.252TyrCys: 0.252 ± 0.016	1.513TyrAsp: 1.513 ± 0.038	1.267TyrGlu: 1.267 ± 0.042	0.883TyrPhe: 0.883 ± 0.031	2.009TyrGly: 2.009 ± 0.05	0.559TyrHis: 0.559 ± 0.021	0.908TyrIle: 0.908 ± 0.027	0.521TyrLys: 0.521 ± 0.024	2.128TyrLeu: 2.128 ± 0.047	0.512TyrMet: 0.512 ± 0.026	0.544TyrAsn: 0.544 ± 0.023	1.041TyrPro: 1.041 ± 0.035	0.596TyrGln: 0.596 ± 0.025	1.709TyrArg: 1.709 ± 0.044	1.073TyrSer: 1.073 ± 0.033	1.068TyrThr: 1.068 ± 0.033	1.46TyrVal: 1.46 ± 0.038	0.361TyrTrp: 0.361 ± 0.021	0.569TyrTyr: 0.569 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3470 proteins (1097512 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---